papain  (Worthington Biochemical)


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    Structured Review

    Worthington Biochemical papain
    Papain, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/papain/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    papain - by Bioz Stars, 2021-10
    94/100 stars

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    Related Articles

    Stripping Membranes:

    Article Title: Role of PARP1 in oligodendrocyte differentiation during developmental myelination and remyelination after myelin damage
    Article Snippet: .. After stripping the meninges, cerebral cortices were enzymatically digested using papain (20 U/ml, LK003176, Worthington) supplemented with DNase I (250 U/ml; D5025, Sigma) and D-(+)-glucose (0.36%; 0188, AMRESCO), and mechanically triturated to obtain single cell. ..

    Centrifugation:

    Article Title: Aβ1-16 controls synaptic vesicle pools at excitatory synapses via cholinergic modulation of synapsin phosphorylation
    Article Snippet: .. Brains were mechanically dissociated by pipetting in a dissociation solution containing 0.127 U/ml of papain (#LK003176, Worthington Biochemical Corporation, Lakewood, New Jersey, USA), 1 mg/ml dispase II (#04942078001, Roche), 0.1 mg/ml DNase I (#LS002139, Worthington), 12.4 mM MgSO4 in HBSS−/−, for 10 min at 37 °C, followed by centrifugation for 5 min at 120 g. Cell suspension was filtered through nylon cell strainers (70 μm), resuspended in Neurobasal A-mix media (Neurobasal A supplemented with 1% (v:v) GlutaMAX™, 1 mM sodium pyruvate, 1% (v:v) Antibiotic/Antimycotic and 2% (v:v) B27), following two additional cycles of mechanical dissociation and centrifugation. ..

    Staining:

    Article Title: Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes
    Article Snippet: .. Materials The following materials were used in this study: dl -2-amino-5-phosphonopentanoic acid (AP-5; ab120271), 6-cyano-7-nitroquinoxaline2,3-dione (CNQX; ab120044), and SMIFH2 (ab218296) from Abcam; bafilomycin A1 (A8627) from Stratech; Neurobasal (21103-049), l -glutamine (25030-024), B27 supplement (17504-044), penicillin-streptomycin (15140-122), Lipofectamine 2000 (11668-019), NeuroTrace 500/525 green fluorescent Nissl stain (N21480), and ProLong Gold antifade mountant (P36930) from Thermo Fisher Scientific; papain (LK003176) from Worthington; fetal calf serum (S1810-500) from Biosera; FluorSave reagent (345789) from Merck Millipore; poly-d -lysine hydrobromide (P7886), d -laminin (L2020), cytosine β-d-arabinofuranoside (C1768), HRP (P8250), 3,3′-diaminobenzidine (D8001), Durcupan resin (44610), protease inhibitor cocktail (P8849), and picrotoxin (P1675) from Sigma-Aldrich. ..

    Fluorsave:

    Article Title: Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes
    Article Snippet: .. Materials The following materials were used in this study: dl -2-amino-5-phosphonopentanoic acid (AP-5; ab120271), 6-cyano-7-nitroquinoxaline2,3-dione (CNQX; ab120044), and SMIFH2 (ab218296) from Abcam; bafilomycin A1 (A8627) from Stratech; Neurobasal (21103-049), l -glutamine (25030-024), B27 supplement (17504-044), penicillin-streptomycin (15140-122), Lipofectamine 2000 (11668-019), NeuroTrace 500/525 green fluorescent Nissl stain (N21480), and ProLong Gold antifade mountant (P36930) from Thermo Fisher Scientific; papain (LK003176) from Worthington; fetal calf serum (S1810-500) from Biosera; FluorSave reagent (345789) from Merck Millipore; poly-d -lysine hydrobromide (P7886), d -laminin (L2020), cytosine β-d-arabinofuranoside (C1768), HRP (P8250), 3,3′-diaminobenzidine (D8001), Durcupan resin (44610), protease inhibitor cocktail (P8849), and picrotoxin (P1675) from Sigma-Aldrich. ..

    Protease Inhibitor:

    Article Title: Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes
    Article Snippet: .. Materials The following materials were used in this study: dl -2-amino-5-phosphonopentanoic acid (AP-5; ab120271), 6-cyano-7-nitroquinoxaline2,3-dione (CNQX; ab120044), and SMIFH2 (ab218296) from Abcam; bafilomycin A1 (A8627) from Stratech; Neurobasal (21103-049), l -glutamine (25030-024), B27 supplement (17504-044), penicillin-streptomycin (15140-122), Lipofectamine 2000 (11668-019), NeuroTrace 500/525 green fluorescent Nissl stain (N21480), and ProLong Gold antifade mountant (P36930) from Thermo Fisher Scientific; papain (LK003176) from Worthington; fetal calf serum (S1810-500) from Biosera; FluorSave reagent (345789) from Merck Millipore; poly-d -lysine hydrobromide (P7886), d -laminin (L2020), cytosine β-d-arabinofuranoside (C1768), HRP (P8250), 3,3′-diaminobenzidine (D8001), Durcupan resin (44610), protease inhibitor cocktail (P8849), and picrotoxin (P1675) from Sigma-Aldrich. ..

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    • papain  (Worthington Biochemical)
    94
    Worthington Biochemical papain
    Papain, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/papain/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    papain - by Bioz Stars, 2021-10
    94/100 stars
    		  Buy from Supplier

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