papain  (Worthington Biochemical)


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    Structured Review

    Worthington Biochemical papain
    Papain, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/papain/product/Worthington Biochemical
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    papain - by Bioz Stars, 2021-07
    95/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Comparison of CRISPR/Cas Endonucleases for in vivo Retinal Gene Editing
    Article Snippet: Images of the retina were collected using an Olympus VS120 Slide Scanner or Perkin Elmer Spinning Disk Confocal Microscope (Zeiss spinning disk, Germany). .. Retinal Dissociation and Flow Cytometry Analysis Retinas were rapidly dissected and digested using a papain dissociation kit (catalog no. LK003176; Worthington Biochemical Corporation, Lakewood, NJ, United States) following the manufacturer’s instructions to obtain a homogenous cell suspension. .. After dissociation, retinal cells were resuspended in FACS buffer (1% Bovine Serum Albumin in Phosphate Buffered Saline) and stained with DAPI (5 μg/mL; catalog no. D1306; Life Technologies Australia) to exclude dead cells.

    Concentration Assay:

    Article Title: Enhancer viruses and a transgenic platform for combinatorial cell subclass-specific labeling
    Article Snippet: .. Where papain was used for dissociations, we added 4.17 mL ACSF (with or without trehalose) to a 125 U vial of Papain (Worthington Biochemical PDS Kit CatLK003176) for a final concentration of 30 U/mL. .. After microdissection, samples were digested in the 30 U/mL papain solution for 30 min at 32°C.

    Staining:

    Article Title: Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes
    Article Snippet: In this procedure, mice were administered a lethal dose of sodium pentobarbital and transcardially perfused with cold phosphate buffer (PB), followed by cold paraformaldehyde (PFA; 4% in 0.1 M PB; performed under license Home Office project license to M. Nolan, PC198F2A0). .. Materials The following materials were used in this study: dl -2-amino-5-phosphonopentanoic acid (AP-5; ab120271), 6-cyano-7-nitroquinoxaline2,3-dione (CNQX; ab120044), and SMIFH2 (ab218296) from Abcam; bafilomycin A1 (A8627) from Stratech; Neurobasal (21103-049), l -glutamine (25030-024), B27 supplement (17504-044), penicillin-streptomycin (15140-122), Lipofectamine 2000 (11668-019), NeuroTrace 500/525 green fluorescent Nissl stain (N21480), and ProLong Gold antifade mountant (P36930) from Thermo Fisher Scientific; papain (LK003176) from Worthington; fetal calf serum (S1810-500) from Biosera; FluorSave reagent (345789) from Merck Millipore; poly-d -lysine hydrobromide (P7886), d -laminin (L2020), cytosine β-d-arabinofuranoside (C1768), HRP (P8250), 3,3′-diaminobenzidine (D8001), Durcupan resin (44610), protease inhibitor cocktail (P8849), and picrotoxin (P1675) from Sigma-Aldrich. ..

    Fluorsave:

    Article Title: Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes
    Article Snippet: In this procedure, mice were administered a lethal dose of sodium pentobarbital and transcardially perfused with cold phosphate buffer (PB), followed by cold paraformaldehyde (PFA; 4% in 0.1 M PB; performed under license Home Office project license to M. Nolan, PC198F2A0). .. Materials The following materials were used in this study: dl -2-amino-5-phosphonopentanoic acid (AP-5; ab120271), 6-cyano-7-nitroquinoxaline2,3-dione (CNQX; ab120044), and SMIFH2 (ab218296) from Abcam; bafilomycin A1 (A8627) from Stratech; Neurobasal (21103-049), l -glutamine (25030-024), B27 supplement (17504-044), penicillin-streptomycin (15140-122), Lipofectamine 2000 (11668-019), NeuroTrace 500/525 green fluorescent Nissl stain (N21480), and ProLong Gold antifade mountant (P36930) from Thermo Fisher Scientific; papain (LK003176) from Worthington; fetal calf serum (S1810-500) from Biosera; FluorSave reagent (345789) from Merck Millipore; poly-d -lysine hydrobromide (P7886), d -laminin (L2020), cytosine β-d-arabinofuranoside (C1768), HRP (P8250), 3,3′-diaminobenzidine (D8001), Durcupan resin (44610), protease inhibitor cocktail (P8849), and picrotoxin (P1675) from Sigma-Aldrich. ..

    Protease Inhibitor:

    Article Title: Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes
    Article Snippet: In this procedure, mice were administered a lethal dose of sodium pentobarbital and transcardially perfused with cold phosphate buffer (PB), followed by cold paraformaldehyde (PFA; 4% in 0.1 M PB; performed under license Home Office project license to M. Nolan, PC198F2A0). .. Materials The following materials were used in this study: dl -2-amino-5-phosphonopentanoic acid (AP-5; ab120271), 6-cyano-7-nitroquinoxaline2,3-dione (CNQX; ab120044), and SMIFH2 (ab218296) from Abcam; bafilomycin A1 (A8627) from Stratech; Neurobasal (21103-049), l -glutamine (25030-024), B27 supplement (17504-044), penicillin-streptomycin (15140-122), Lipofectamine 2000 (11668-019), NeuroTrace 500/525 green fluorescent Nissl stain (N21480), and ProLong Gold antifade mountant (P36930) from Thermo Fisher Scientific; papain (LK003176) from Worthington; fetal calf serum (S1810-500) from Biosera; FluorSave reagent (345789) from Merck Millipore; poly-d -lysine hydrobromide (P7886), d -laminin (L2020), cytosine β-d-arabinofuranoside (C1768), HRP (P8250), 3,3′-diaminobenzidine (D8001), Durcupan resin (44610), protease inhibitor cocktail (P8849), and picrotoxin (P1675) from Sigma-Aldrich. ..

    Incubation:

    Article Title: A Method for the Isolation and Culture of Adult Rat Retinal Pigment Epithelial (RPE) Cells to Study Retinal Diseases
    Article Snippet: .. The eyes were then incubated in a 20 U/ml papain solution (Worthington PDS Kit) for up to 1 h at 37°C in a 24-well-plate. ..

    Article Title: Astragalus polysaccharide attenuates metabolic memory-triggered ER stress and apoptosis via regulation of miR-204/SIRT1 axis in retinal pigment epithelial cells
    Article Snippet: .. The eye was then incubated in a 24-well plate containing 20 U/ml papain solution (Worthington PDS Kit, Lakewood, NJ, U.S.A.) for 1 h at 37°C. ..

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  • 95
    Worthington Biochemical papain
    Papain, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/papain/product/Worthington Biochemical
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    papain - by Bioz Stars, 2021-07
    95/100 stars
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