calpain 1  (Boster Bio)


Bioz Verified Symbol Boster Bio is a verified supplier
Bioz Manufacturer Symbol Boster Bio manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91

    Structured Review

    Boster Bio calpain 1
    Calpain expression and activity. ( a ) The expressions of <t>calpain-1,</t> calpain-2, and the 145 kDa stable breakdown products of spectrin α-II were detected with Western blot. ( b ) Relative density of calpain-1, calpain-2, and stable breakdown products compared with β-actin. Data are shown as the fold changes over the control group. n = 4 for each group. ( c ) Calpain activity measured using a fluorescence substrate N-succinyl-LLVY-AMC. Data are shown as the fold changes over the control group. n = 3 for each group. Statistical analyses were conducted using one-way analysis of variance followed by the Tukey test. * P
    Calpain 1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calpain 1/product/Boster Bio
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calpain 1 - by Bioz Stars, 2022-05
    91/100 stars

    Images

    1) Product Images from "Calpain inhibition improves erectile function in diabetic mice via upregulating endothelial nitric oxide synthase expression and reducing apoptosis"

    Article Title: Calpain inhibition improves erectile function in diabetic mice via upregulating endothelial nitric oxide synthase expression and reducing apoptosis

    Journal: Asian Journal of Andrology

    doi: 10.4103/aja.aja_63_17

    Calpain expression and activity. ( a ) The expressions of calpain-1, calpain-2, and the 145 kDa stable breakdown products of spectrin α-II were detected with Western blot. ( b ) Relative density of calpain-1, calpain-2, and stable breakdown products compared with β-actin. Data are shown as the fold changes over the control group. n = 4 for each group. ( c ) Calpain activity measured using a fluorescence substrate N-succinyl-LLVY-AMC. Data are shown as the fold changes over the control group. n = 3 for each group. Statistical analyses were conducted using one-way analysis of variance followed by the Tukey test. * P
    Figure Legend Snippet: Calpain expression and activity. ( a ) The expressions of calpain-1, calpain-2, and the 145 kDa stable breakdown products of spectrin α-II were detected with Western blot. ( b ) Relative density of calpain-1, calpain-2, and stable breakdown products compared with β-actin. Data are shown as the fold changes over the control group. n = 4 for each group. ( c ) Calpain activity measured using a fluorescence substrate N-succinyl-LLVY-AMC. Data are shown as the fold changes over the control group. n = 3 for each group. Statistical analyses were conducted using one-way analysis of variance followed by the Tukey test. * P

    Techniques Used: Expressing, Activity Assay, Western Blot, Fluorescence

    2) Product Images from "Calpain inhibition improves erectile function in diabetic mice via upregulating endothelial nitric oxide synthase expression and reducing apoptosis"

    Article Title: Calpain inhibition improves erectile function in diabetic mice via upregulating endothelial nitric oxide synthase expression and reducing apoptosis

    Journal: Asian Journal of Andrology

    doi: 10.4103/aja.aja_63_17

    Calpain expression and activity. ( a ) The expressions of calpain-1, calpain-2, and the 145 kDa stable breakdown products of spectrin α-II were detected with Western blot. ( b ) Relative density of calpain-1, calpain-2, and stable breakdown products compared with β-actin. Data are shown as the fold changes over the control group. n = 4 for each group. ( c ) Calpain activity measured using a fluorescence substrate N-succinyl-LLVY-AMC. Data are shown as the fold changes over the control group. n = 3 for each group. Statistical analyses were conducted using one-way analysis of variance followed by the Tukey test. * P
    Figure Legend Snippet: Calpain expression and activity. ( a ) The expressions of calpain-1, calpain-2, and the 145 kDa stable breakdown products of spectrin α-II were detected with Western blot. ( b ) Relative density of calpain-1, calpain-2, and stable breakdown products compared with β-actin. Data are shown as the fold changes over the control group. n = 4 for each group. ( c ) Calpain activity measured using a fluorescence substrate N-succinyl-LLVY-AMC. Data are shown as the fold changes over the control group. n = 3 for each group. Statistical analyses were conducted using one-way analysis of variance followed by the Tukey test. * P

    Techniques Used: Expressing, Activity Assay, Western Blot, Fluorescence

    3) Product Images from "Over-Expression of Calpastatin Inhibits Calpain Activation and Attenuates Post-Infarction Myocardial Remodeling"

    Article Title: Over-Expression of Calpastatin Inhibits Calpain Activation and Attenuates Post-Infarction Myocardial Remodeling

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0120178

    Over-expression of CAST suppresses calpain expression and activity. (A) Representative Western blots of calpain-1, calpain-2 and calpastatin (CAST) of the heart tissue from the CAST transgenic (TG) mice and wild-type (WT) littermates subjected to sham and myocardial infarction (MI) surgery. (B) Calpain activity in the myocardial tissue. Data are expressed as means ± SE. **P
    Figure Legend Snippet: Over-expression of CAST suppresses calpain expression and activity. (A) Representative Western blots of calpain-1, calpain-2 and calpastatin (CAST) of the heart tissue from the CAST transgenic (TG) mice and wild-type (WT) littermates subjected to sham and myocardial infarction (MI) surgery. (B) Calpain activity in the myocardial tissue. Data are expressed as means ± SE. **P

    Techniques Used: Over Expression, Expressing, Activity Assay, Western Blot, Transgenic Assay, Mouse Assay

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Boster Bio calpain 1
    Calpain expression and activity. ( a ) The expressions of <t>calpain-1,</t> calpain-2, and the 145 kDa stable breakdown products of spectrin α-II were detected with Western blot. ( b ) Relative density of calpain-1, calpain-2, and stable breakdown products compared with β-actin. Data are shown as the fold changes over the control group. n = 4 for each group. ( c ) Calpain activity measured using a fluorescence substrate N-succinyl-LLVY-AMC. Data are shown as the fold changes over the control group. n = 3 for each group. Statistical analyses were conducted using one-way analysis of variance followed by the Tukey test. * P
    Calpain 1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calpain 1/product/Boster Bio
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calpain 1 - by Bioz Stars, 2022-05
    91/100 stars
      Buy from Supplier

    Image Search Results


    Calpain expression and activity. ( a ) The expressions of calpain-1, calpain-2, and the 145 kDa stable breakdown products of spectrin α-II were detected with Western blot. ( b ) Relative density of calpain-1, calpain-2, and stable breakdown products compared with β-actin. Data are shown as the fold changes over the control group. n = 4 for each group. ( c ) Calpain activity measured using a fluorescence substrate N-succinyl-LLVY-AMC. Data are shown as the fold changes over the control group. n = 3 for each group. Statistical analyses were conducted using one-way analysis of variance followed by the Tukey test. * P

    Journal: Asian Journal of Andrology

    Article Title: Calpain inhibition improves erectile function in diabetic mice via upregulating endothelial nitric oxide synthase expression and reducing apoptosis

    doi: 10.4103/aja.aja_63_17

    Figure Lengend Snippet: Calpain expression and activity. ( a ) The expressions of calpain-1, calpain-2, and the 145 kDa stable breakdown products of spectrin α-II were detected with Western blot. ( b ) Relative density of calpain-1, calpain-2, and stable breakdown products compared with β-actin. Data are shown as the fold changes over the control group. n = 4 for each group. ( c ) Calpain activity measured using a fluorescence substrate N-succinyl-LLVY-AMC. Data are shown as the fold changes over the control group. n = 3 for each group. Statistical analyses were conducted using one-way analysis of variance followed by the Tukey test. * P

    Article Snippet: 40 μg protein lysate of each sample was loaded on 10% sodium dodecyl sulfate/polyacrylamide (SDS-PAGE) gel for electrophoresis at 60 V for 30 min and 110 V for 60 min. Then, the proteins on the gel were transferred to polyvinylidene difluoride membrane at 200 mA for 2 h. After being blocked with 5% bovine serum albumin (BSA) for 2 h, the membranes were incubated with the following antibodies at 4°C overnight: spectrin α-II (1:100, sc-48382, Santa Cruz Biotechnology, Dallas, TX, USA); calpain-1 (1:500, BA0679, Boster, Wuhan, China); calpain-2 (1:500, BA1575, Boster); eNOS (1:500, 610296, BD Biosciences, San Jose, CAUSA); phospho-eNOS (Ser1177; 1:1000, 9570, CST, Danvers, MA, USA); caspase-3 (1:1000, 9665, CST); cleaved caspase-3 (1:1000, 9664, CST); or β-actin (1:500, BM0627, Boster).

    Techniques: Expressing, Activity Assay, Western Blot, Fluorescence

    Calpain expression and activity. ( a ) The expressions of calpain-1, calpain-2, and the 145 kDa stable breakdown products of spectrin α-II were detected with Western blot. ( b ) Relative density of calpain-1, calpain-2, and stable breakdown products compared with β-actin. Data are shown as the fold changes over the control group. n = 4 for each group. ( c ) Calpain activity measured using a fluorescence substrate N-succinyl-LLVY-AMC. Data are shown as the fold changes over the control group. n = 3 for each group. Statistical analyses were conducted using one-way analysis of variance followed by the Tukey test. * P

    Journal: Asian Journal of Andrology

    Article Title: Calpain inhibition improves erectile function in diabetic mice via upregulating endothelial nitric oxide synthase expression and reducing apoptosis

    doi: 10.4103/aja.aja_63_17

    Figure Lengend Snippet: Calpain expression and activity. ( a ) The expressions of calpain-1, calpain-2, and the 145 kDa stable breakdown products of spectrin α-II were detected with Western blot. ( b ) Relative density of calpain-1, calpain-2, and stable breakdown products compared with β-actin. Data are shown as the fold changes over the control group. n = 4 for each group. ( c ) Calpain activity measured using a fluorescence substrate N-succinyl-LLVY-AMC. Data are shown as the fold changes over the control group. n = 3 for each group. Statistical analyses were conducted using one-way analysis of variance followed by the Tukey test. * P

    Article Snippet: 40 μg protein lysate of each sample was loaded on 10% sodium dodecyl sulfate/polyacrylamide (SDS-PAGE) gel for electrophoresis at 60 V for 30 min and 110 V for 60 min. Then, the proteins on the gel were transferred to polyvinylidene difluoride membrane at 200 mA for 2 h. After being blocked with 5% bovine serum albumin (BSA) for 2 h, the membranes were incubated with the following antibodies at 4°C overnight: spectrin α-II (1:100, sc-48382, Santa Cruz Biotechnology, Dallas, TX, USA); calpain-1 (1:500, BA0679, Boster, Wuhan, China); calpain-2 (1:500, BA1575, Boster); eNOS (1:500, 610296, BD Biosciences, San Jose, CAUSA); phospho-eNOS (Ser1177; 1:1000, 9570, CST, Danvers, MA, USA); caspase-3 (1:1000, 9665, CST); cleaved caspase-3 (1:1000, 9664, CST); or β-actin (1:500, BM0627, Boster).

    Techniques: Expressing, Activity Assay, Western Blot, Fluorescence

    Over-expression of CAST suppresses calpain expression and activity. (A) Representative Western blots of calpain-1, calpain-2 and calpastatin (CAST) of the heart tissue from the CAST transgenic (TG) mice and wild-type (WT) littermates subjected to sham and myocardial infarction (MI) surgery. (B) Calpain activity in the myocardial tissue. Data are expressed as means ± SE. **P

    Journal: PLoS ONE

    Article Title: Over-Expression of Calpastatin Inhibits Calpain Activation and Attenuates Post-Infarction Myocardial Remodeling

    doi: 10.1371/journal.pone.0120178

    Figure Lengend Snippet: Over-expression of CAST suppresses calpain expression and activity. (A) Representative Western blots of calpain-1, calpain-2 and calpastatin (CAST) of the heart tissue from the CAST transgenic (TG) mice and wild-type (WT) littermates subjected to sham and myocardial infarction (MI) surgery. (B) Calpain activity in the myocardial tissue. Data are expressed as means ± SE. **P

    Article Snippet: Membranes were blocked with TBS-T containing 5% skim powdered milk for 1 hour and then incubated with anti-calpain-1 (1:200; BA0679, Boster, Wuhan, China), anti-calpain-2 (1:200; BA1575, Boster, Wuhan, China), anti-CAST (1:200; SC-7561, Santa Cruz Biotechnology, Dallas, TX, USA), anti-matrix metalloproteinase (MMP)-2 (1:200; BA0569, Boster, Wuhan, China), anti-MMP-9 (1:200; BA2202, Boster, Wuhan, China), anti-tissue inhibitor of MMP (TIMP)-1 (1:200; BA3727, Boster, Wuhan, China), anti-TIMP-2 (1:200; BA0576, Boster, Wuhan, China), anti-TGF-β (1: 500; ab66043, Cambridge, MA, USA) and GAPDH (1:200; BA2913, Boster, Wuhan, China) antibodies overnight.

    Techniques: Over Expression, Expressing, Activity Assay, Western Blot, Transgenic Assay, Mouse Assay