endoproteinase aspn  (New England Biolabs)


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  • 82
    Name:
    Endoproteinase AspN
    Description:
    Endoproteinase AspN 50 ug
    Catalog Number:
    p8104s
    Price:
    76
    Size:
    50 ug
    Category:
    Proteases
    Buy from Supplier


    Structured Review

    New England Biolabs endoproteinase aspn
    Endoproteinase AspN
    Endoproteinase AspN 50 ug
    https://www.bioz.com/result/endoproteinase aspn/product/New England Biolabs
    Average 82 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    endoproteinase aspn - by Bioz Stars, 2020-01
    82/100 stars

    Images

    1) Product Images from "Refinement of Glucagon-like Peptide 1 Docking to Its Intact Receptor Using Mid-region Photolabile Probes and Molecular Modeling"

    Article Title: Refinement of Glucagon-like Peptide 1 Docking to Its Intact Receptor Using Mid-region Photolabile Probes and Molecular Modeling

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.217901

    Endoproteinase Asp-N cleavage of the GLP1 receptor labeled with the Bpa 16 probe. Shown at the left is a diagram of the predicted sites of Asp-N cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph
    Figure Legend Snippet: Endoproteinase Asp-N cleavage of the GLP1 receptor labeled with the Bpa 16 probe. Shown at the left is a diagram of the predicted sites of Asp-N cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph

    Techniques Used: Labeling, Autoradiography

    Endoproteinase Asp-N cleavage of the GLP1 receptor labeled with the Bpa 20 probe. Shown at the left is a diagram of the predicted sites of Asp-N cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph
    Figure Legend Snippet: Endoproteinase Asp-N cleavage of the GLP1 receptor labeled with the Bpa 20 probe. Shown at the left is a diagram of the predicted sites of Asp-N cleavage of the GLP1 receptor along with the masses. Shown at the right is a representative autoradiograph

    Techniques Used: Labeling, Autoradiography

    2) Product Images from "Molecular Basis of Glucagon-like Peptide 1 Docking to Its Intact Receptor Studied with Carboxyl-terminal Photolabile Probes *"

    Article Title: Molecular Basis of Glucagon-like Peptide 1 Docking to Its Intact Receptor Studied with Carboxyl-terminal Photolabile Probes *

    Journal:

    doi: 10.1074/jbc.M109.038109

    Further localization of the domain of Bpa 35 probe labeling. Left shows that endoproteinase Asp-N cleavage of the M r = 15,000 fragment resulting from Lys-C cleavage of the wild-type receptor yielded a band migrating at approximate M r = 4,000. Right shows
    Figure Legend Snippet: Further localization of the domain of Bpa 35 probe labeling. Left shows that endoproteinase Asp-N cleavage of the M r = 15,000 fragment resulting from Lys-C cleavage of the wild-type receptor yielded a band migrating at approximate M r = 4,000. Right shows

    Techniques Used: Labeling

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Macrocyclized Extended Peptides: Inhibiting the Substrate-Recognition Domain of Tankyrase
    Article Snippet: Proteolytic Degradation Endoproteinase AspN (50 ng, New England BioLabs) was added to a mixture of TAMRA-labeled peptide (400 μM), TAMRA (100 μM), and Endoproteinase AspN enzyme buffer (New England BioLabs), and the volume was made up to 50 μL with sterile water. .. At each time point, a 5 μL aliquot was taken and centrifuged at 10,000 rpm at 4 °C, and the supernatant was diluted 4 times with water before being loaded onto an analytical HPLC (Agilent 1260 Infinity, Supelcosil ABZ+PLUS column (150 mm × 4.6 mm, 3 μm)) and eluted with a linear gradient system (solvent A: 0.05% (v/v) TFA in water; solvent B: 0.05% (v/v) TFA in acetonitrile).

    Clone Assay:

    Article Title: High Divergence of the Precursor Peptides in Combinatorial Lanthipeptide Biosynthesis
    Article Snippet: Restriction endonucleases, DNA polymerases, T4 DNA ligase, and endoproteinase Asp-N and Glu-C were purchased from New England Biolabs. .. E. coli DH5α was used as host for cloning and plasmid propagation, and E. coli BL21 (DE3) was used as a host for coexpression.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Improvement of the glycoproteomic toolbox with the discovery of a unique C-terminal cleavage specificity of flavastacin for N-glycosylated asparagine
    Article Snippet: Enzymes used for digestion were trypsin (Trypsin Sequencing Grade Modified; V5111) from Promega and endoproteinase AspN (AspN; P8104S) from New England Biolabs (Flavastacin, purified host cell protein, Uniprot Q47899; see the corresponding SDS-gel for purity of AspN (Flavastacin) in Supplementary Figure ). .. For LC-MS solvents, water was further purified using the LC-Pak Polisher from Merck Millipore.

    Article Title: Assessing in vivo dynamics of multiple quality attributes from a therapeutic IgG4 monoclonal antibody circulating in cynomolgus monkey
    Article Snippet: Reagents Trypsin (sequencing grade, V5111 and V5117) was purchased from Promega (Madison, WI); Lysyl Endopeptidase (Mass Spectrometry Grade, 125–05061) was purchased from Wako Chemicals (Richmond, VA); Pepsin (Porcine stomach mucosa, 02195367) was purchased from MP Biomedicals (Solon, OH); Endoproteinase AspN was purchased from New England BioLabs Inc. (P8104S) and Roche Diagnostics (11054589001). .. Phosphate-buffered saline 10X Solution (PBS, BP399-500), Tris buffer (45-000-236), 1.0 N hydrochloric acid solution (HCl, SA48-500), tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl, PI-20490), Progenta Anionic Acid Labile Surfactant II (AALS II, 5 mg, 50-121-8307), Cynomolgus monkey serum (027-AS-MP-5ML), LC/MS grade water (W6-4), acetonitrile (ACN, A996-4), formic acid (FA, optima LC/MS grade, A117-50) and Pierce Spin Columns (69705) were obtained from Fisher Scientific (Fairlawn, NJ).

    Purification:

    Article Title: Improvement of the glycoproteomic toolbox with the discovery of a unique C-terminal cleavage specificity of flavastacin for N-glycosylated asparagine
    Article Snippet: .. Enzymes used for digestion were trypsin (Trypsin Sequencing Grade Modified; V5111) from Promega and endoproteinase AspN (AspN; P8104S) from New England Biolabs (Flavastacin, purified host cell protein, Uniprot Q47899; see the corresponding SDS-gel for purity of AspN (Flavastacin) in Supplementary Figure ). .. All buffer and solutions were prepared with deionized and purified water (dH2 O) using a Milli-Q water purification system (18.2 MΩ · cm−1 at 25°C, total organic carbon of 3 ppb) from Merck Millipore.

    SDS-Gel:

    Article Title: Improvement of the glycoproteomic toolbox with the discovery of a unique C-terminal cleavage specificity of flavastacin for N-glycosylated asparagine
    Article Snippet: .. Enzymes used for digestion were trypsin (Trypsin Sequencing Grade Modified; V5111) from Promega and endoproteinase AspN (AspN; P8104S) from New England Biolabs (Flavastacin, purified host cell protein, Uniprot Q47899; see the corresponding SDS-gel for purity of AspN (Flavastacin) in Supplementary Figure ). .. All buffer and solutions were prepared with deionized and purified water (dH2 O) using a Milli-Q water purification system (18.2 MΩ · cm−1 at 25°C, total organic carbon of 3 ppb) from Merck Millipore.

    Sequencing:

    Article Title: Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3) *
    Article Snippet: .. We therefore attempted to use endoproteinase AspN in order to further digest trypsin-generated O-glycopeptides derived from monoclonal IgG3 from “SCDTPPPCPR” to “SC” and “DTPPPCPR.” However, while the majority of the nonglycosylated tryptic hinge repeat peptides were cleaved by AspN, the O-glycopeptides with the same peptide sequence remained undigested. ..

    Article Title: Improvement of the glycoproteomic toolbox with the discovery of a unique C-terminal cleavage specificity of flavastacin for N-glycosylated asparagine
    Article Snippet: .. Enzymes used for digestion were trypsin (Trypsin Sequencing Grade Modified; V5111) from Promega and endoproteinase AspN (AspN; P8104S) from New England Biolabs (Flavastacin, purified host cell protein, Uniprot Q47899; see the corresponding SDS-gel for purity of AspN (Flavastacin) in Supplementary Figure ). .. All buffer and solutions were prepared with deionized and purified water (dH2 O) using a Milli-Q water purification system (18.2 MΩ · cm−1 at 25°C, total organic carbon of 3 ppb) from Merck Millipore.

    Article Title: Assessing in vivo dynamics of multiple quality attributes from a therapeutic IgG4 monoclonal antibody circulating in cynomolgus monkey
    Article Snippet: .. Reagents Trypsin (sequencing grade, V5111 and V5117) was purchased from Promega (Madison, WI); Lysyl Endopeptidase (Mass Spectrometry Grade, 125–05061) was purchased from Wako Chemicals (Richmond, VA); Pepsin (Porcine stomach mucosa, 02195367) was purchased from MP Biomedicals (Solon, OH); Endoproteinase AspN was purchased from New England BioLabs Inc. (P8104S) and Roche Diagnostics (11054589001). .. Dithiothreitol (DTT, D5545), iodoacetamide (IAA, I6125) and N-ethylmaleimide (NEM, E3876–5G) were purchased from Sigma-Aldrich (St. Louis, MO).

    Incubation:

    Article Title: Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3) *
    Article Snippet: Alternatively, several IgG3 samples were digested in solution without reduction alkylation: 3 μg of IgG3 was incubated with 0.3 μg of trypsin in a total volume of 25 μl 25 mm ABC at 37 °C overnight. .. Endoproteinase AspN (New England Biolabs, Ipswich, MA) was used to further digest trypsin-generated (glyco)peptides.

    other:

    Article Title: Molecular Basis of Glucagon-like Peptide 1 Docking to Its Intact Receptor Studied with Carboxyl-terminal Photolabile Probes *
    Article Snippet: Endoproteinase Lys-C (Lys-C) was from Roche Applied Science (Indianapolis, IN) and endoproteinase Asp-N (Asp-N) was from New England Biolabs Inc. (Ipswich, MA).

    Article Title: Refinement of Glucagon-like Peptide 1 Docking to Its Intact Receptor Using Mid-region Photolabile Probes and Molecular Modeling
    Article Snippet: Endoproteinase Glu-C (Glu-C) and endoproteinase Asp-N (Asp-N) were from New England Biolabs Inc. (Ipswich, MA).

    Plasmid Preparation:

    Article Title: A thymidylate synthase ternary complex-specific antibody, FTS, permits functional monitoring of fluoropyrimidines dosing
    Article Snippet: Glutathione-sepharose 4B, proteinG-sepharose column and pGEX-2TK plasmid were from GE Life Sciences. .. Calf intestinal phosphatase (CIP) and Endoproteinase AspN were from New England BioLabs.

    Article Title: High Divergence of the Precursor Peptides in Combinatorial Lanthipeptide Biosynthesis
    Article Snippet: Restriction endonucleases, DNA polymerases, T4 DNA ligase, and endoproteinase Asp-N and Glu-C were purchased from New England Biolabs. .. E. coli DH5α was used as host for cloning and plasmid propagation, and E. coli BL21 (DE3) was used as a host for coexpression.

    Mass Spectrometry:

    Article Title: Assessing in vivo dynamics of multiple quality attributes from a therapeutic IgG4 monoclonal antibody circulating in cynomolgus monkey
    Article Snippet: .. Reagents Trypsin (sequencing grade, V5111 and V5117) was purchased from Promega (Madison, WI); Lysyl Endopeptidase (Mass Spectrometry Grade, 125–05061) was purchased from Wako Chemicals (Richmond, VA); Pepsin (Porcine stomach mucosa, 02195367) was purchased from MP Biomedicals (Solon, OH); Endoproteinase AspN was purchased from New England BioLabs Inc. (P8104S) and Roche Diagnostics (11054589001). .. Dithiothreitol (DTT, D5545), iodoacetamide (IAA, I6125) and N-ethylmaleimide (NEM, E3876–5G) were purchased from Sigma-Aldrich (St. Louis, MO).

    Modification:

    Article Title: Improvement of the glycoproteomic toolbox with the discovery of a unique C-terminal cleavage specificity of flavastacin for N-glycosylated asparagine
    Article Snippet: .. Enzymes used for digestion were trypsin (Trypsin Sequencing Grade Modified; V5111) from Promega and endoproteinase AspN (AspN; P8104S) from New England Biolabs (Flavastacin, purified host cell protein, Uniprot Q47899; see the corresponding SDS-gel for purity of AspN (Flavastacin) in Supplementary Figure ). .. All buffer and solutions were prepared with deionized and purified water (dH2 O) using a Milli-Q water purification system (18.2 MΩ · cm−1 at 25°C, total organic carbon of 3 ppb) from Merck Millipore.

    SDS Page:

    Article Title: Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3) *
    Article Snippet: Paragraph title: SDS-PAGE Analysis and Proteolytic Digestion ... Endoproteinase AspN (New England Biolabs, Ipswich, MA) was used to further digest trypsin-generated (glyco)peptides.

    Derivative Assay:

    Article Title: Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3) *
    Article Snippet: .. We therefore attempted to use endoproteinase AspN in order to further digest trypsin-generated O-glycopeptides derived from monoclonal IgG3 from “SCDTPPPCPR” to “SC” and “DTPPPCPR.” However, while the majority of the nonglycosylated tryptic hinge repeat peptides were cleaved by AspN, the O-glycopeptides with the same peptide sequence remained undigested. ..

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  • Team
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  • 82
    New England Biolabs endoproteinase aspn
    Endoproteinase Aspn, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endoproteinase aspn/product/New England Biolabs
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    endoproteinase aspn - by Bioz Stars, 2020-01
    82/100 stars
      Buy from Supplier

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