a1 3 4 fucosidase  (New England Biolabs)


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    Name:
    a1 3 4 Fucosidase
    Description:
    a1 3 4 Fucosidase 1 000 units
    Catalog Number:
    P0769L
    Price:
    510
    Category:
    Glycosidases
    Size:
    1 000 units
    Buy from Supplier


    Structured Review

    New England Biolabs a1 3 4 fucosidase
    a1 3 4 Fucosidase
    a1 3 4 Fucosidase 1 000 units
    https://www.bioz.com/result/a1 3 4 fucosidase/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a1 3 4 fucosidase - by Bioz Stars, 2021-06
    93/100 stars

    Images

    1) Product Images from "The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells"

    Article Title: The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0199128

    The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p
    Figure Legend Snippet: The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p

    Techniques Used: Modification, Staining, Flow Cytometry, Cytometry, Inhibition, Quantitation Assay

    2) Product Images from "The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells"

    Article Title: The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0199128

    The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p
    Figure Legend Snippet: The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p

    Techniques Used: Modification, Staining, Flow Cytometry, Cytometry, Inhibition, Quantitation Assay

    Related Articles

    Incubation:

    Article Title: Sialyl Lewisx-P-selectin cascade mediates tumor–mesothelial adhesion in ascitic fluid shear flow
    Article Snippet: .. In some experiments, M-CSCs were incubated with or without neuramidase (100 mU, N2876, Sigma-Aldrich), α1,3/4-fucosidase (150 mU, P0769S, New England BioLabs), OSGE (100 μg mL−1 , CLE100, Cedarlane) or PNGase (10 U mL−1 , P0704S, New England BioLabs) according to the manufacturer’s instructions for 1 h at 37 °C prior staining. .. To inhibit sulfation, M-CSCs were incubated with sodium chlorate (25 mM , 403016, Sigma-Aldrich) in serum-free medium for 48 h. To functionally block sLex , sLea or IGF-1R on M-CSCs, M-CSCs were incubated with CSLEX-1 (10 μg mL−1 , 551344, BD Biosciences), anti-CA19–9(10 μg mL−1 , ab15146, Abcam), anti-IGF-1R (2 μg mL−1 , 24–60, Invitrogen) or isotype antibodies for 1 h at 37 °C, respectively.

    Article Title: The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells
    Article Snippet: Coverslips were fixed in 4% PFA every 15 minutes and images for quantitation and representation were acquired as mentioned. .. After cell dissociation using enzyme free solution, cells were washed three times in 1xPBS, then incubated in PBS with 0.08U/mL fucosidase (New England Biolabs, α-1,2:P0724S, α-1,3/4:P0769S) for 30 minutes at 37°C. ..

    Article Title: The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells
    Article Snippet: Coverslips were fixed in 4% PFA every 15 minutes and images for quantitation and representation were acquired as mentioned. .. Fucosidase treatment After cell dissociation using enzyme free solution, cells were washed three times in 1xPBS, then incubated in PBS with 0.08U/mL fucosidase (New England Biolabs, α-1,2:P0724S, α-1,3/4:P0769S) for 30 minutes at 37°C. ..

    Staining:

    Article Title: Sialyl Lewisx-P-selectin cascade mediates tumor–mesothelial adhesion in ascitic fluid shear flow
    Article Snippet: .. In some experiments, M-CSCs were incubated with or without neuramidase (100 mU, N2876, Sigma-Aldrich), α1,3/4-fucosidase (150 mU, P0769S, New England BioLabs), OSGE (100 μg mL−1 , CLE100, Cedarlane) or PNGase (10 U mL−1 , P0704S, New England BioLabs) according to the manufacturer’s instructions for 1 h at 37 °C prior staining. .. To inhibit sulfation, M-CSCs were incubated with sodium chlorate (25 mM , 403016, Sigma-Aldrich) in serum-free medium for 48 h. To functionally block sLex , sLea or IGF-1R on M-CSCs, M-CSCs were incubated with CSLEX-1 (10 μg mL−1 , 551344, BD Biosciences), anti-CA19–9(10 μg mL−1 , ab15146, Abcam), anti-IGF-1R (2 μg mL−1 , 24–60, Invitrogen) or isotype antibodies for 1 h at 37 °C, respectively.

    other:

    Article Title: A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
    Article Snippet: The exoglycosidases and associated product numbers used in this study are: β1-4 Galactosidase S (P0745S); α1-3,6 Galactosidase (P0731S); α1-3,4 Fucosidase (P0769S); and α1-2 Fucosidase (P0724S); α1-3,4,6 Galactosidase (P0747S); β-N-Acetylglucosaminidase S (P0744S); α2-3 Neuraminidase S (P0743S); and α2-3,6,8 Neuraminidase (P0720S).

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    New England Biolabs a1 3 4 fucosidase
    The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and <t>fucosidase</t> treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of <t>α-1,3/4</t> fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p
    A1 3 4 Fucosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a1 3 4 fucosidase/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a1 3 4 fucosidase - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    Image Search Results


    The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p

    Journal: PLoS ONE

    Article Title: The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells

    doi: 10.1371/journal.pone.0199128

    Figure Lengend Snippet: The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p

    Article Snippet: Fucosidase treatment After cell dissociation using enzyme free solution, cells were washed three times in 1xPBS, then incubated in PBS with 0.08U/mL fucosidase (New England Biolabs, α-1,2:P0724S, α-1,3/4:P0769S) for 30 minutes at 37°C.

    Techniques: Modification, Staining, Flow Cytometry, Cytometry, Inhibition, Quantitation Assay

    The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p

    Journal: PLoS ONE

    Article Title: The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells

    doi: 10.1371/journal.pone.0199128

    Figure Lengend Snippet: The fucose salvage pathway inhibits invadopodia through α1–2 fucosylation. A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p

    Article Snippet: After cell dissociation using enzyme free solution, cells were washed three times in 1xPBS, then incubated in PBS with 0.08U/mL fucosidase (New England Biolabs, α-1,2:P0724S, α-1,3/4:P0769S) for 30 minutes at 37°C.

    Techniques: Modification, Staining, Flow Cytometry, Cytometry, Inhibition, Quantitation Assay