β1 4 galactosidase s  (New England Biolabs)


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    New England Biolabs β1 4 galactosidase s
    β1 4 Galactosidase S, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    β1 4 galactosidase s  (New England Biolabs)


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    New England Biolabs β1 4 galactosidase s
    β1 4 Galactosidase S, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    β1 4 galactosidase s  (New England Biolabs)


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    New England Biolabs β1 4 galactosidase s
    β1 4 Galactosidase S, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    β1 4 galactosidase s  (New England Biolabs)


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    New England Biolabs β1 4 galactosidase s
    β1 4 Galactosidase S, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p0745s a2 3 6 8 neuraminidase new england biolabs  (New England Biolabs)


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    New England Biolabs p0745s a2 3 6 8 neuraminidase new england biolabs
    P0745s A2 3 6 8 Neuraminidase New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    β1 4 galactosidase s  (New England Biolabs)


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    New England Biolabs β1 4 galactosidase s
    β1 4 Galactosidase S, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    β1 4 galactosidase s  (New England Biolabs)


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    New England Biolabs β1 4 galactosidase s
    S. carnosus-sasG COL (SasG-I) and S. carnosus-sasG MW2 (SasG-II) were tested for adhesion to corneocytes following pre-incubation with ( A ) PNGase F, ( B ) O-Glycosidase, ( C ) α1-2,3,6 Mannosidase, ( D ) α1-3,4 Fucosidase, ( E ) β- N - Acetylglucosaminidase S, ( F ) <t>β1-4</t> Galactosidase S, and ( G ) α2-3,6,8 Neuraminidase. The percent area of adhesion in 10 images from three independent experiments (n = 30) was measured with Fiji ImageJ and analyzed in GraphPad Prism. Statistical significance was analyzed using the unpaired t-test or non-parametric Mann-Whitney test for data with non-normal distribution (**** P <0.0001).
    β1 4 Galactosidase S, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β1 4 galactosidase s/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    β1 4 galactosidase s - by Bioz Stars, 2024-05
    94/100 stars

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    1) Product Images from "Staphylococcus aureus skin colonization is mediated by SasG lectin variation"

    Article Title: Staphylococcus aureus skin colonization is mediated by SasG lectin variation

    Journal: bioRxiv

    doi: 10.1101/2023.11.20.567970

    S. carnosus-sasG COL (SasG-I) and S. carnosus-sasG MW2 (SasG-II) were tested for adhesion to corneocytes following pre-incubation with ( A ) PNGase F, ( B ) O-Glycosidase, ( C ) α1-2,3,6 Mannosidase, ( D ) α1-3,4 Fucosidase, ( E ) β- N - Acetylglucosaminidase S, ( F ) β1-4 Galactosidase S, and ( G ) α2-3,6,8 Neuraminidase. The percent area of adhesion in 10 images from three independent experiments (n = 30) was measured with Fiji ImageJ and analyzed in GraphPad Prism. Statistical significance was analyzed using the unpaired t-test or non-parametric Mann-Whitney test for data with non-normal distribution (**** P <0.0001).
    Figure Legend Snippet: S. carnosus-sasG COL (SasG-I) and S. carnosus-sasG MW2 (SasG-II) were tested for adhesion to corneocytes following pre-incubation with ( A ) PNGase F, ( B ) O-Glycosidase, ( C ) α1-2,3,6 Mannosidase, ( D ) α1-3,4 Fucosidase, ( E ) β- N - Acetylglucosaminidase S, ( F ) β1-4 Galactosidase S, and ( G ) α2-3,6,8 Neuraminidase. The percent area of adhesion in 10 images from three independent experiments (n = 30) was measured with Fiji ImageJ and analyzed in GraphPad Prism. Statistical significance was analyzed using the unpaired t-test or non-parametric Mann-Whitney test for data with non-normal distribution (**** P <0.0001).

    Techniques Used: Incubation, MANN-WHITNEY

    S. carnosus -pALC2073 EV, SasG-I-expressing S. carnosus-sasG COL and SasG-II-expressing S. carnosus-sasG MW2 , and SasG-II-expressing S. carnosus with the A-domain deleted ( S. carnosus-sasG MW2ΔA ) at an MOI of 5 were tested for adhesion to either differentiated ( A and B ) or undifferentiated ( C and D ) N/TERT keratinocytes. ( A ) Adhesion to terminally differentiated cells as shown by overall percent adhesion. ( B ) Adhesion to terminally differentiated cells as shown by percent cell association to pALC2073- sasG MW2 input inoculum. Both SasG-expressing strains adhered more to differentiated cells than the EV and A domain mutant controls. ( C ) Adhesion to a monolayer of undifferentiated cells as shown by overall percent adhesion. ( D ) Adhesion to a monolayer of undifferentiated cells as shown by percent cell association to pALC2073- sasG MW2 input inoculum. There were no significant differences in adhesion between the EV and A domain mutant controls and the SasG-expressing strains. ( A-D ) The CFU/mL of three independent experiments (n = 3) were calculated and analyzed for statistical significance in GraphPad Prism using ordinary one-way ANOVA. ( E ) Data from panels A and C displaying differences in adhesion between differentiated and undifferentiated N/TERT keratinocytes for SasG-I-expressing S. carnosus-sasG COL and SasG-II-expressing S. carnosus-sasG MW2 . Both strains adhered well to differentiated N/TERT keratinocytes, and did not adhere well to undifferentiated N/TERT keratinocytes. No statistical analyses were performed for Panel E. SasG-I-expressing S. carnosus-sasG COL and SasG-II-expressing S. carnosus-sasG MW2 were tested for overall percent adhesion to differentiated N/TERT keratinocytes following treatment with ( F ) β- N - Acetylglucosaminidase S and ( G ) β1-4 Galactosidase S. β1-4 Galactosidase S reduced adhesion of both strains, while β- N -Acetylglucosaminidase S resulted in a greater reduction in adhesion of S. carnosus-sasG MW2 . The CFU/mL of three independent experiments (n = 3) were calculated and analyzed for statistical significance in GraphPad Prism using an unpaired t-test. * P =0.0500.
    Figure Legend Snippet: S. carnosus -pALC2073 EV, SasG-I-expressing S. carnosus-sasG COL and SasG-II-expressing S. carnosus-sasG MW2 , and SasG-II-expressing S. carnosus with the A-domain deleted ( S. carnosus-sasG MW2ΔA ) at an MOI of 5 were tested for adhesion to either differentiated ( A and B ) or undifferentiated ( C and D ) N/TERT keratinocytes. ( A ) Adhesion to terminally differentiated cells as shown by overall percent adhesion. ( B ) Adhesion to terminally differentiated cells as shown by percent cell association to pALC2073- sasG MW2 input inoculum. Both SasG-expressing strains adhered more to differentiated cells than the EV and A domain mutant controls. ( C ) Adhesion to a monolayer of undifferentiated cells as shown by overall percent adhesion. ( D ) Adhesion to a monolayer of undifferentiated cells as shown by percent cell association to pALC2073- sasG MW2 input inoculum. There were no significant differences in adhesion between the EV and A domain mutant controls and the SasG-expressing strains. ( A-D ) The CFU/mL of three independent experiments (n = 3) were calculated and analyzed for statistical significance in GraphPad Prism using ordinary one-way ANOVA. ( E ) Data from panels A and C displaying differences in adhesion between differentiated and undifferentiated N/TERT keratinocytes for SasG-I-expressing S. carnosus-sasG COL and SasG-II-expressing S. carnosus-sasG MW2 . Both strains adhered well to differentiated N/TERT keratinocytes, and did not adhere well to undifferentiated N/TERT keratinocytes. No statistical analyses were performed for Panel E. SasG-I-expressing S. carnosus-sasG COL and SasG-II-expressing S. carnosus-sasG MW2 were tested for overall percent adhesion to differentiated N/TERT keratinocytes following treatment with ( F ) β- N - Acetylglucosaminidase S and ( G ) β1-4 Galactosidase S. β1-4 Galactosidase S reduced adhesion of both strains, while β- N -Acetylglucosaminidase S resulted in a greater reduction in adhesion of S. carnosus-sasG MW2 . The CFU/mL of three independent experiments (n = 3) were calculated and analyzed for statistical significance in GraphPad Prism using an unpaired t-test. * P =0.0500.

    Techniques Used: Expressing, Mutagenesis

    β1 4 galactosidase s  (New England Biolabs)


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    New England Biolabs β1 4 galactosidase s
    β1 4 Galactosidase S, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    b1 4 galactosidase neb  (New England Biolabs)


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    New England Biolabs b1 4 galactosidase neb
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    b1 4 galactosidase  (New England Biolabs)


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    New England Biolabs b1 4 galactosidase
    B1 4 Galactosidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p0745l b1 3 galactosidase neb  (New England Biolabs)


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    New England Biolabs p0745l b1 3 galactosidase neb
    P0745l B1 3 Galactosidase Neb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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