heparinase ii  (New England Biolabs)


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  • 93
    Name:
    Bacteroides Heparinase II
    Description:
    Bacteroides Heparinase II 200 units
    Catalog Number:
    p0736l
    Price:
    470
    Size:
    200 units
    Category:
    Glycosidases
    Buy from Supplier


    Structured Review

    New England Biolabs heparinase ii
    Bacteroides Heparinase II
    Bacteroides Heparinase II 200 units
    https://www.bioz.com/result/heparinase ii/product/New England Biolabs
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    heparinase ii - by Bioz Stars, 2020-05
    93/100 stars

    Images

    1) Product Images from "C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells"

    Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0153723

    Effect of heparin sodium salt, sodium chlorate and heparinase II treatment in binding assay. (a) Western blotting analysis following treatment with heparin sodium salt. Relative intensity of immunofluorescence signal derived from different binding assays (b) heparin sodium salt, (c) sodium chlorate and (d) heparinase II. Mean fluorescence intensity of stained cells was measured with ImageJ. Error bars represent standard deviation. *, p
    Figure Legend Snippet: Effect of heparin sodium salt, sodium chlorate and heparinase II treatment in binding assay. (a) Western blotting analysis following treatment with heparin sodium salt. Relative intensity of immunofluorescence signal derived from different binding assays (b) heparin sodium salt, (c) sodium chlorate and (d) heparinase II. Mean fluorescence intensity of stained cells was measured with ImageJ. Error bars represent standard deviation. *, p

    Techniques Used: Binding Assay, Western Blot, Immunofluorescence, Derivative Assay, Fluorescence, Staining, Standard Deviation

    2) Product Images from "Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis. Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis"

    Article Title: Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis. Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis

    Journal: Physiological Reports

    doi: 10.14814/phy2.13334

    LDL binding to the vessel wall in vitro following treatment with Site B peptide or enzymatic digestion of proteoglycan GAG chains. Tissue sections of carotid arteries from wild‐type mice with intimal hyperplasia were incubated with human LDL . Bound LDL was detected using anti‐apoB antibody. (A) LDL binding to tissue sections pre‐incubated with positively charged SiteB peptide (white circles) or neutrally charged SiteB KE peptide (gray circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). (B) LDL binding to tissue sections pre‐treated with the GAG ‐degrading enzymes chondroitinase (white circles) or heparinase (white dot circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). Data was analyzed using Mann–Whitney rank sum test. Graph bars show median values. P
    Figure Legend Snippet: LDL binding to the vessel wall in vitro following treatment with Site B peptide or enzymatic digestion of proteoglycan GAG chains. Tissue sections of carotid arteries from wild‐type mice with intimal hyperplasia were incubated with human LDL . Bound LDL was detected using anti‐apoB antibody. (A) LDL binding to tissue sections pre‐incubated with positively charged SiteB peptide (white circles) or neutrally charged SiteB KE peptide (gray circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). (B) LDL binding to tissue sections pre‐treated with the GAG ‐degrading enzymes chondroitinase (white circles) or heparinase (white dot circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). Data was analyzed using Mann–Whitney rank sum test. Graph bars show median values. P

    Techniques Used: Binding Assay, In Vitro, Mouse Assay, Incubation, MANN-WHITNEY

    Related Articles

    Concentration Assay:

    Article Title: Role of high endothelial venule-expressed heparan sulfate in chemokine presentation and lymphocyte homing
    Article Snippet: .. Mice were injected intravenously with a mixture of Bacteroides heparinase I, heparinase II and heparinase III (New England BioLabs; heparinase I, heparinase II and heparinase III at a concentration of 75, 75 and 15 mU/g mouse body weight, respectively) in PBS. .. Four hours after the injection, the mice were injected intravenously with biotinylated-rabbit anti-mouse CCL21 (0.2 µg/g mouse body weight; PeproTech).

    Article Title: A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors
    Article Snippet: .. 100 μL Bacteroides Heparinase II (New England Biolabs) diluted in PBS was added to the well at a concentration of 2 U/mL, and cells were incubated at 37°C for 1 h. Cells were then washed twice in PBS and used in the viral fusion assay as described. .. Cells were imaged on a Zeiss LSM 510 laser scanning confocal microscope equipped with a 20× objective and far red and diode (405 nm) lasers using ZEN software (Carl Zeiss).

    Injection:

    Article Title: Role of high endothelial venule-expressed heparan sulfate in chemokine presentation and lymphocyte homing
    Article Snippet: .. Mice were injected intravenously with a mixture of Bacteroides heparinase I, heparinase II and heparinase III (New England BioLabs; heparinase I, heparinase II and heparinase III at a concentration of 75, 75 and 15 mU/g mouse body weight, respectively) in PBS. .. Four hours after the injection, the mice were injected intravenously with biotinylated-rabbit anti-mouse CCL21 (0.2 µg/g mouse body weight; PeproTech).

    Single Vesicle Fusion Assay:

    Article Title: A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors
    Article Snippet: .. 100 μL Bacteroides Heparinase II (New England Biolabs) diluted in PBS was added to the well at a concentration of 2 U/mL, and cells were incubated at 37°C for 1 h. Cells were then washed twice in PBS and used in the viral fusion assay as described. .. Cells were imaged on a Zeiss LSM 510 laser scanning confocal microscope equipped with a 20× objective and far red and diode (405 nm) lasers using ZEN software (Carl Zeiss).

    Incubation:

    Article Title: A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors
    Article Snippet: .. 100 μL Bacteroides Heparinase II (New England Biolabs) diluted in PBS was added to the well at a concentration of 2 U/mL, and cells were incubated at 37°C for 1 h. Cells were then washed twice in PBS and used in the viral fusion assay as described. .. Cells were imaged on a Zeiss LSM 510 laser scanning confocal microscope equipped with a 20× objective and far red and diode (405 nm) lasers using ZEN software (Carl Zeiss).

    Mouse Assay:

    Article Title: Role of high endothelial venule-expressed heparan sulfate in chemokine presentation and lymphocyte homing
    Article Snippet: .. Mice were injected intravenously with a mixture of Bacteroides heparinase I, heparinase II and heparinase III (New England BioLabs; heparinase I, heparinase II and heparinase III at a concentration of 75, 75 and 15 mU/g mouse body weight, respectively) in PBS. .. Four hours after the injection, the mice were injected intravenously with biotinylated-rabbit anti-mouse CCL21 (0.2 µg/g mouse body weight; PeproTech).

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  • 93
    New England Biolabs heparinase ii
    Effect of heparin sodium salt, sodium chlorate and <t>heparinase</t> II treatment in binding assay. (a) Western blotting analysis following treatment with heparin sodium salt. Relative intensity of immunofluorescence signal derived from different binding assays (b) heparin sodium salt, (c) sodium chlorate and (d) heparinase II. Mean fluorescence intensity of stained cells was measured with ImageJ. Error bars represent standard deviation. *, p
    Heparinase Ii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heparinase ii/product/New England Biolabs
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    heparinase ii - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    Effect of heparin sodium salt, sodium chlorate and heparinase II treatment in binding assay. (a) Western blotting analysis following treatment with heparin sodium salt. Relative intensity of immunofluorescence signal derived from different binding assays (b) heparin sodium salt, (c) sodium chlorate and (d) heparinase II. Mean fluorescence intensity of stained cells was measured with ImageJ. Error bars represent standard deviation. *, p

    Journal: PLoS ONE

    Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells

    doi: 10.1371/journal.pone.0153723

    Figure Lengend Snippet: Effect of heparin sodium salt, sodium chlorate and heparinase II treatment in binding assay. (a) Western blotting analysis following treatment with heparin sodium salt. Relative intensity of immunofluorescence signal derived from different binding assays (b) heparin sodium salt, (c) sodium chlorate and (d) heparinase II. Mean fluorescence intensity of stained cells was measured with ImageJ. Error bars represent standard deviation. *, p

    Article Snippet: Heparinase II treatment of LMH cells LMH cells were pre-treated in Heparinase Reaction Buffer (20 mM Tris-HCl, 100 mM NaCl and 1.5 mM CaCl2 ) with heparinase II (10U/ml) from Bacteroides (New England Biolabs GmbH, Frankfurt am Main, Germany) or PBS as control for 2h at 37°C in an atmosphere supplied with 5% CO2 .

    Techniques: Binding Assay, Western Blot, Immunofluorescence, Derivative Assay, Fluorescence, Staining, Standard Deviation

    LDL binding to the vessel wall in vitro following treatment with Site B peptide or enzymatic digestion of proteoglycan GAG chains. Tissue sections of carotid arteries from wild‐type mice with intimal hyperplasia were incubated with human LDL . Bound LDL was detected using anti‐apoB antibody. (A) LDL binding to tissue sections pre‐incubated with positively charged SiteB peptide (white circles) or neutrally charged SiteB KE peptide (gray circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). (B) LDL binding to tissue sections pre‐treated with the GAG ‐degrading enzymes chondroitinase (white circles) or heparinase (white dot circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). Data was analyzed using Mann–Whitney rank sum test. Graph bars show median values. P

    Journal: Physiological Reports

    Article Title: Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis. Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis

    doi: 10.14814/phy2.13334

    Figure Lengend Snippet: LDL binding to the vessel wall in vitro following treatment with Site B peptide or enzymatic digestion of proteoglycan GAG chains. Tissue sections of carotid arteries from wild‐type mice with intimal hyperplasia were incubated with human LDL . Bound LDL was detected using anti‐apoB antibody. (A) LDL binding to tissue sections pre‐incubated with positively charged SiteB peptide (white circles) or neutrally charged SiteB KE peptide (gray circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). (B) LDL binding to tissue sections pre‐treated with the GAG ‐degrading enzymes chondroitinase (white circles) or heparinase (white dot circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). Data was analyzed using Mann–Whitney rank sum test. Graph bars show median values. P

    Article Snippet: For GAG enzyme experiments, tissue sections were pre‐incubated 30 min at RT with Heparinase II (New England BioLabs, P0736S) or Chondrotinase ABC (Sigma, C3667) as per manufacturer's recommendations.

    Techniques: Binding Assay, In Vitro, Mouse Assay, Incubation, MANN-WHITNEY