Journal: PPAR Research
Article Title: α-Bisabolol Mitigates Colon Inflammation by Stimulating Colon PPAR-γ Transcription Factor: In Vivo and In Vitro Study
Figure Lengend Snippet: Effect of bisabolol on proinflammatory cytokines and mediator protein and mRNA expression. DSS administration significantly elevated proinflammatory cytokine concentrations. α -Bisabolol treatment significantly prevented the increase in proinflammatory cytokines at the protein (IL-6 (a), IL-1 β (b), TNF- α (c), and IL-17A (d)) and mRNA ((IL-6 (e), IL-1 β (f), TNF- α (g), and IL-17A (h)) levels. DSS administration also increased COX-2 and iNOS protein levels. α -Bisabolol treatment significantly downregulated protein expression (i, j). DSS administration significantly increased tissue nitrite levels, and α -bisabolol treatment prevented this increase (k). The density of immunoblots normalized to GAPDH. Eight ( n = 8) animals used for ELISA, six ( n = 6) animals used for mRNA, and six ( n = 6) animals used for Western blot and tissue nitrite level measurements. The data is expressed as mean ± SEM. ∗ p
Article Snippet: Colonic mucosa was homogenized with phosphatase and protease inhibitor cocktail tablet (cat#A32959, Thermo-Scientific, USA) dissolved in RIPA buffer (Cat# 20-188, Millipore, St. Louis, MO, USA) with zirconium beads (2 mm, Cat# 11079124zx, Biospec, Bartlesville, OK, USA) in Precellys 24 tissue homogenizer (Bertin Instruments, Montigny-le-Bretonneux, France).
Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay