pardaxin  (Alomone Labs)


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    Alomone Labs pardaxin
    Pardaxin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pardaxin/product/Alomone Labs
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pardaxin - by Bioz Stars, 2022-12
    86/100 stars

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    Alomone Labs p kv4 2
    Dysregulations of protein synthesis capacity in aged mice. Western blot performed on hippocampal tissues demonstrated that compared to young mice, old mice exhibited: (A) Increased levels of mTOR phosphorylation (Ser2448); (B) Increased levels of p70S6K phosphorylation (Thr389); (C) Unaltered levels of 4EBP1 phosphorylation; (D) Increased levels of AKT phosphorylation (Ser473); (E) Increased levels of GSK3β (Ser9); (F) Increased levels of eEF2 phosphorylation (Thr56); (G) Increased levels of AMPKα phosphorylation (Thr172); (H) Unaltered levels of <t>Kv4.2</t> phosphorylation; (I) Decreased levels of GluA1 expression. Except for GluA1, no change on levels of total proteins was observed between young and old mice. n = 7 for young mice and n = 6 for old mice (representative bands from three mice per group). ∗ p
    P Kv4 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p kv4 2/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p kv4 2 - by Bioz Stars, 2022-12
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    86
    Alomone Labs rabbit anti aqp5
    Knockdown of p300 but not CBP in primary cells decreases expression of AT1 cell differentiation marker <t>Aqp5</t> . Primary rat AEC were transduced on day 2 in culture with p300 shRNA or pGIPZ non-silencing ( NS ) control virus or Cbp shRNA or pLKO.1 non-silencing (NS) control virus. Cells were harvested for RNA and protein 72 h posttransduction (on day 5 in culture). A, qRT-PCR of p300 and Aqp5 mRNA levels following transduction with p300 shRNA lentivirus compared with pGIPZ NS control virus. n = 4. *, p
    Rabbit Anti Aqp5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Dysregulations of protein synthesis capacity in aged mice. Western blot performed on hippocampal tissues demonstrated that compared to young mice, old mice exhibited: (A) Increased levels of mTOR phosphorylation (Ser2448); (B) Increased levels of p70S6K phosphorylation (Thr389); (C) Unaltered levels of 4EBP1 phosphorylation; (D) Increased levels of AKT phosphorylation (Ser473); (E) Increased levels of GSK3β (Ser9); (F) Increased levels of eEF2 phosphorylation (Thr56); (G) Increased levels of AMPKα phosphorylation (Thr172); (H) Unaltered levels of Kv4.2 phosphorylation; (I) Decreased levels of GluA1 expression. Except for GluA1, no change on levels of total proteins was observed between young and old mice. n = 7 for young mice and n = 6 for old mice (representative bands from three mice per group). ∗ p

    Journal: Frontiers in Aging Neuroscience

    Article Title: Memory Decline and Behavioral Inflexibility in Aged Mice Are Correlated With Dysregulation of Protein Synthesis Capacity

    doi: 10.3389/fnagi.2019.00246

    Figure Lengend Snippet: Dysregulations of protein synthesis capacity in aged mice. Western blot performed on hippocampal tissues demonstrated that compared to young mice, old mice exhibited: (A) Increased levels of mTOR phosphorylation (Ser2448); (B) Increased levels of p70S6K phosphorylation (Thr389); (C) Unaltered levels of 4EBP1 phosphorylation; (D) Increased levels of AKT phosphorylation (Ser473); (E) Increased levels of GSK3β (Ser9); (F) Increased levels of eEF2 phosphorylation (Thr56); (G) Increased levels of AMPKα phosphorylation (Thr172); (H) Unaltered levels of Kv4.2 phosphorylation; (I) Decreased levels of GluA1 expression. Except for GluA1, no change on levels of total proteins was observed between young and old mice. n = 7 for young mice and n = 6 for old mice (representative bands from three mice per group). ∗ p

    Article Snippet: Membranes were probed overnight at 4°C using primary antibodies for the following antibodies: p-Kv4.2 (1:300; Santa Cruz); Kv4.2 (1:500; Alomone Labs); GluA1 (1:1000; Cell Signaling); p-mTOR (Ser2448) (1:1000; Cell Signaling); mTOR (1:1000; Cell Signaling); p-p70S6K (Thr389) (1:1000; Cell Signaling); p70S6K (1:1000; Cell Signaling); p-4EBP1 (Thr37/46) (1:1000; Cell Signaling); 4EBP1 (1:1000; Cell Signaling); p-AKT (Ser473) (1:1000; Cell Signaling); AKT (1:1000; Cell Signaling); p-GSK3β (Ser9) (1:1000; Cell Signaling); GSK3β (1:1000; Cell Signaling); p-eIF2α (Ser51) (1:1000; Cell Signaling); eIF2α (1:1000; Cell Signaling); p-eEF2 (Thr56) (1:1000; Cell Signaling); eEF2 (1:1000; Cell Signaling); p-AMPKα (Thr172) (1:1000; Cell Signaling); AMPKα (1:1000; Cell Signaling); AMPKα1 (1:1000; Cell Signaling); AMPKα2 (1:1000; Cell Signaling); p-ERK (1:1000; Cell Signaling), ERK (1:1000; Cell Signaling), β-actin (1:5000; Sigma-Aldrich).

    Techniques: Mouse Assay, Western Blot, Expressing

    Knockdown of p300 but not CBP in primary cells decreases expression of AT1 cell differentiation marker Aqp5 . Primary rat AEC were transduced on day 2 in culture with p300 shRNA or pGIPZ non-silencing ( NS ) control virus or Cbp shRNA or pLKO.1 non-silencing (NS) control virus. Cells were harvested for RNA and protein 72 h posttransduction (on day 5 in culture). A, qRT-PCR of p300 and Aqp5 mRNA levels following transduction with p300 shRNA lentivirus compared with pGIPZ NS control virus. n = 4. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: p300/β-Catenin Interactions Regulate Adult Progenitor Cell Differentiation Downstream of WNT5a/Protein Kinase C (PKC) *

    doi: 10.1074/jbc.M115.706416

    Figure Lengend Snippet: Knockdown of p300 but not CBP in primary cells decreases expression of AT1 cell differentiation marker Aqp5 . Primary rat AEC were transduced on day 2 in culture with p300 shRNA or pGIPZ non-silencing ( NS ) control virus or Cbp shRNA or pLKO.1 non-silencing (NS) control virus. Cells were harvested for RNA and protein 72 h posttransduction (on day 5 in culture). A, qRT-PCR of p300 and Aqp5 mRNA levels following transduction with p300 shRNA lentivirus compared with pGIPZ NS control virus. n = 4. *, p

    Article Snippet: Primary antibodies (Abs) included rabbit anti-AQP5 (Alomone Labs, Jerusalem, Israel) -p300, -CBP, -Ser(P)-89, -Lamin A/C (Santa Cruz Biotechnology, Santa Cruz, CA), -RAGE, -CAV-1 (Abcam, Cambridge, MA), -pan phospho-PKC (Cell Signaling, Danvers, MA), -T1α (Affinity BioReagents, Golden, CO), -AQP5, pro-SFTPC (Millipore, Temecula, CA), and mouse anti-p300/CBP (NM11) (Santa Cruz Biotechnology), -GAPDH (Life Technologies), and -β-catenin (Sigma).

    Techniques: Expressing, Cell Differentiation, Marker, shRNA, Quantitative RT-PCR, Transduction

    Wnt5a −/− mice exhibit reduced expression of AT1 cell differentiation markers and activated PKC. A, qRT-PCR of AT1 cell markers Aqp5 , Rage , T1 α, and Cav-1 and AT2 cell marker pro-Sftpc at E18.5. WT: n = 3. Wnt5a −/− : n = 4. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: p300/β-Catenin Interactions Regulate Adult Progenitor Cell Differentiation Downstream of WNT5a/Protein Kinase C (PKC) *

    doi: 10.1074/jbc.M115.706416

    Figure Lengend Snippet: Wnt5a −/− mice exhibit reduced expression of AT1 cell differentiation markers and activated PKC. A, qRT-PCR of AT1 cell markers Aqp5 , Rage , T1 α, and Cav-1 and AT2 cell marker pro-Sftpc at E18.5. WT: n = 3. Wnt5a −/− : n = 4. *, p

    Article Snippet: Primary antibodies (Abs) included rabbit anti-AQP5 (Alomone Labs, Jerusalem, Israel) -p300, -CBP, -Ser(P)-89, -Lamin A/C (Santa Cruz Biotechnology, Santa Cruz, CA), -RAGE, -CAV-1 (Abcam, Cambridge, MA), -pan phospho-PKC (Cell Signaling, Danvers, MA), -T1α (Affinity BioReagents, Golden, CO), -AQP5, pro-SFTPC (Millipore, Temecula, CA), and mouse anti-p300/CBP (NM11) (Santa Cruz Biotechnology), -GAPDH (Life Technologies), and -β-catenin (Sigma).

    Techniques: Mouse Assay, Expressing, Cell Differentiation, Quantitative RT-PCR, Marker