gfap  (Alomone Labs)


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    Alomone Labs gfap
    Upregulation <t>of</t> <t>P75</t> NTR in transgenic retinas after Müller cell ablation. (A-I) Double-label immunostaining for GS (A, D and G) and P75 NTR (B, E and H) in control ( Ctl , A-C ) and transgenic ( TG , D-I ) mice 7d and 14d after tamoxifen treatment. The retina of control mice showed weak immunoreactivity of P75 NTR (A-C) . TG mice showed upregulation of P75 NTR predominantly by surviving Müller cells 7d (D-F) and 14d (G-I) after TMX-induced Müller cell ablation. Small arrows in (D and G) point to regions of Müller cell loss, and large arrows in (G) indicate defects in the outer limiting membrane following Müller cell ablation. GCL = ganglion cell layer, INL = inner nuclear layer, ONL = outer nuclear layer. (J and K) Western blots showed significant upregulation of <t>GFAP</t> concomitantly with overexpression of P75 NTR 7d and 14d after Müller cell ablation in TG mice. † P < 0.05 and * P < 0.01, TG vs. control, N = 4–8 in each group. Scale bars: A-I , 50 μm.
    Gfap, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    gfap - by Bioz Stars, 2023-01
    86/100 stars

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    1) Product Images from "Involvement of NT3 and P75 NTR in photoreceptor degeneration following selective Müller cell ablation"

    Article Title: Involvement of NT3 and P75 NTR in photoreceptor degeneration following selective Müller cell ablation

    Journal: Journal of Neuroinflammation

    doi: 10.1186/1742-2094-10-137

    Upregulation of P75 NTR in transgenic retinas after Müller cell ablation. (A-I) Double-label immunostaining for GS (A, D and G) and P75 NTR (B, E and H) in control ( Ctl , A-C ) and transgenic ( TG , D-I ) mice 7d and 14d after tamoxifen treatment. The retina of control mice showed weak immunoreactivity of P75 NTR (A-C) . TG mice showed upregulation of P75 NTR predominantly by surviving Müller cells 7d (D-F) and 14d (G-I) after TMX-induced Müller cell ablation. Small arrows in (D and G) point to regions of Müller cell loss, and large arrows in (G) indicate defects in the outer limiting membrane following Müller cell ablation. GCL = ganglion cell layer, INL = inner nuclear layer, ONL = outer nuclear layer. (J and K) Western blots showed significant upregulation of GFAP concomitantly with overexpression of P75 NTR 7d and 14d after Müller cell ablation in TG mice. † P < 0.05 and * P < 0.01, TG vs. control, N = 4–8 in each group. Scale bars: A-I , 50 μm.
    Figure Legend Snippet: Upregulation of P75 NTR in transgenic retinas after Müller cell ablation. (A-I) Double-label immunostaining for GS (A, D and G) and P75 NTR (B, E and H) in control ( Ctl , A-C ) and transgenic ( TG , D-I ) mice 7d and 14d after tamoxifen treatment. The retina of control mice showed weak immunoreactivity of P75 NTR (A-C) . TG mice showed upregulation of P75 NTR predominantly by surviving Müller cells 7d (D-F) and 14d (G-I) after TMX-induced Müller cell ablation. Small arrows in (D and G) point to regions of Müller cell loss, and large arrows in (G) indicate defects in the outer limiting membrane following Müller cell ablation. GCL = ganglion cell layer, INL = inner nuclear layer, ONL = outer nuclear layer. (J and K) Western blots showed significant upregulation of GFAP concomitantly with overexpression of P75 NTR 7d and 14d after Müller cell ablation in TG mice. † P < 0.05 and * P < 0.01, TG vs. control, N = 4–8 in each group. Scale bars: A-I , 50 μm.

    Techniques Used: Transgenic Assay, Immunostaining, Western Blot, Over Expression, Mouse Assay

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