pbs  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc pbs
    Pbs, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs/product/Gold Biotechnology Inc
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    pbs - by Bioz Stars, 2022-09
    94/100 stars

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    Gold Biotechnology Inc 1x pbs
    Glutamine deprivation promotes autophagy and ammonia inhibits autophagy. (A) Primary murine chondrocytes were cultured in media containing 100% glutamine or 0% glutamine for 24 hours. Cells were then treated with IL-1β (10 ng/mL) in the presence or absence of chloroquine (10 μM) for 24 hours. Protein expression of p62 and LC3-II were measured by immunoblotting. (B) Primary murine chondrocytes were plated on coated cover slips cultured in glutamine containing or glutamine free media for 12 hours. Cells were treated with chloroquine (10 μM) for 6 hours. Cells were fixed with 4% formaldehyde in <t>PBS</t> and immunofluorescence (IF) was performed for LC3B and p62. Cells were mounted on slides and imaged with representative images displayed. (C) Primary chondrocytes were cultured in media containing 4 mM or 0 mM glutamine. Cells were supplemented with ammonium chloride at the indicated concentrations. After 6 hours, cells were treated with IL-1β (10 ng/mL) for 24 hours. Immunoblotting was performed for p62 and LC3B to display autophagosome processing. Image displays representative experiment. (D) Primary chondrocytes were cultured in media containing 4 mM or 0 mM glutamine. Cells were supplemented with glutamate (200 μM). After 6 hours, cells were treated with IL-1β (10 ng/mL) for 24 hours. Immunoblotting was performed for p62 and LC3b. Image displays representative experiment. (E) Primary murine chondrocytes were plated on coated cover slips cultured in glutamine containing or glutamine free media for 12 hours. Cells were supplemented with ammonium chloride (2 mM) or glutamate (200 μM). Cells were fixed with 4% formaldehyde and IF was performed for LC3B and p62. Cells were mounted on slides and imaged with representative images to display autophagosome punctate.
    1x Pbs, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x pbs/product/Gold Biotechnology Inc
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    1x pbs - by Bioz Stars, 2022-09
    94/100 stars
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    Glutamine deprivation promotes autophagy and ammonia inhibits autophagy. (A) Primary murine chondrocytes were cultured in media containing 100% glutamine or 0% glutamine for 24 hours. Cells were then treated with IL-1β (10 ng/mL) in the presence or absence of chloroquine (10 μM) for 24 hours. Protein expression of p62 and LC3-II were measured by immunoblotting. (B) Primary murine chondrocytes were plated on coated cover slips cultured in glutamine containing or glutamine free media for 12 hours. Cells were treated with chloroquine (10 μM) for 6 hours. Cells were fixed with 4% formaldehyde in PBS and immunofluorescence (IF) was performed for LC3B and p62. Cells were mounted on slides and imaged with representative images displayed. (C) Primary chondrocytes were cultured in media containing 4 mM or 0 mM glutamine. Cells were supplemented with ammonium chloride at the indicated concentrations. After 6 hours, cells were treated with IL-1β (10 ng/mL) for 24 hours. Immunoblotting was performed for p62 and LC3B to display autophagosome processing. Image displays representative experiment. (D) Primary chondrocytes were cultured in media containing 4 mM or 0 mM glutamine. Cells were supplemented with glutamate (200 μM). After 6 hours, cells were treated with IL-1β (10 ng/mL) for 24 hours. Immunoblotting was performed for p62 and LC3b. Image displays representative experiment. (E) Primary murine chondrocytes were plated on coated cover slips cultured in glutamine containing or glutamine free media for 12 hours. Cells were supplemented with ammonium chloride (2 mM) or glutamate (200 μM). Cells were fixed with 4% formaldehyde and IF was performed for LC3B and p62. Cells were mounted on slides and imaged with representative images to display autophagosome punctate.

    Journal: bioRxiv

    Article Title: Glutamine metabolism modulates chondrocyte inflammatory response

    doi: 10.1101/2022.06.09.495504

    Figure Lengend Snippet: Glutamine deprivation promotes autophagy and ammonia inhibits autophagy. (A) Primary murine chondrocytes were cultured in media containing 100% glutamine or 0% glutamine for 24 hours. Cells were then treated with IL-1β (10 ng/mL) in the presence or absence of chloroquine (10 μM) for 24 hours. Protein expression of p62 and LC3-II were measured by immunoblotting. (B) Primary murine chondrocytes were plated on coated cover slips cultured in glutamine containing or glutamine free media for 12 hours. Cells were treated with chloroquine (10 μM) for 6 hours. Cells were fixed with 4% formaldehyde in PBS and immunofluorescence (IF) was performed for LC3B and p62. Cells were mounted on slides and imaged with representative images displayed. (C) Primary chondrocytes were cultured in media containing 4 mM or 0 mM glutamine. Cells were supplemented with ammonium chloride at the indicated concentrations. After 6 hours, cells were treated with IL-1β (10 ng/mL) for 24 hours. Immunoblotting was performed for p62 and LC3B to display autophagosome processing. Image displays representative experiment. (D) Primary chondrocytes were cultured in media containing 4 mM or 0 mM glutamine. Cells were supplemented with glutamate (200 μM). After 6 hours, cells were treated with IL-1β (10 ng/mL) for 24 hours. Immunoblotting was performed for p62 and LC3b. Image displays representative experiment. (E) Primary murine chondrocytes were plated on coated cover slips cultured in glutamine containing or glutamine free media for 12 hours. Cells were supplemented with ammonium chloride (2 mM) or glutamate (200 μM). Cells were fixed with 4% formaldehyde and IF was performed for LC3B and p62. Cells were mounted on slides and imaged with representative images to display autophagosome punctate.

    Article Snippet: Cells were washed twice with 1x PBS and lysed using 1x luciferase lysis buffer (L-740, GoldBio, St. Louis, MO USA).

    Techniques: Cell Culture, Expressing, Immunofluorescence