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Cell Signaling Technology Inc p38
Phosphorylation of <t>p38,</t> ERK1/2, and JNK by BK and DABK in human amnion fibroblasts. (A–C) Time-dependent effects of BK (0.1 μM) on p38, ERK1/2, and JNK phosphorylation. (D–F) Time-dependent effects of DABK (0.1 μM) on p38, ERK1/2, and JNK phosphorylation. n = 3–4. Statistical analysis was performed with one-way ANOVA test followed by Dunnett’s test. Top panels are the representative immunoblots. * p
P38, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "The Bradykinin System Contributes to the Regulation of Prostaglandin-Endoperoxide Synthase 2 Expression in Human Amnion Fibroblasts: Implications for Term and Preterm Birth"

Article Title: The Bradykinin System Contributes to the Regulation of Prostaglandin-Endoperoxide Synthase 2 Expression in Human Amnion Fibroblasts: Implications for Term and Preterm Birth

Journal: Frontiers in Endocrinology

doi: 10.3389/fendo.2022.873727

Phosphorylation of p38, ERK1/2, and JNK by BK and DABK in human amnion fibroblasts. (A–C) Time-dependent effects of BK (0.1 μM) on p38, ERK1/2, and JNK phosphorylation. (D–F) Time-dependent effects of DABK (0.1 μM) on p38, ERK1/2, and JNK phosphorylation. n = 3–4. Statistical analysis was performed with one-way ANOVA test followed by Dunnett’s test. Top panels are the representative immunoblots. * p
Figure Legend Snippet: Phosphorylation of p38, ERK1/2, and JNK by BK and DABK in human amnion fibroblasts. (A–C) Time-dependent effects of BK (0.1 μM) on p38, ERK1/2, and JNK phosphorylation. (D–F) Time-dependent effects of DABK (0.1 μM) on p38, ERK1/2, and JNK phosphorylation. n = 3–4. Statistical analysis was performed with one-way ANOVA test followed by Dunnett’s test. Top panels are the representative immunoblots. * p

Techniques Used: Western Blot

Role of ERK1/2, p38, and JNK in BK- and DABK-induced PTGS2 expression in human amnion fibroblasts. (A, B) BK (0.1 μM, 4 h)-induced PTGS2 expression was blocked by the ERK1/2 inhibitor PD98059 (20 µM) (mRNA: n = 4; protein: n = 3) or the p38MAPK inhibitor SB203580 (10 µM) (mRNA: n = 4; protein: n = 3). (C, D) DABK (0.1 μM, 4 h)-induced PTGS2 expression was blocked by the ERK1/2 inhibitor PD98059 (20 µM) (mRNA: n = 3; protein: n = 4) or the p38MAPK inhibitor SB203580 (10 µM) (mRNA: n = 3; protein: n = 4). (E, F) The JNK inhibitor SP600125 (10 µM) failed to block the induction of PTGS2 expression by BK (0.1 μM, 4 h) (mRNA: n = 4; protein: n = 3) or by DABK (0.1 μM, 4 h) (mRNA: n = 5; protein: n = 3). Statistical analysis was performed with one-way ANOVA test followed by Tukey test. Top panels are the representative immunoblots. * p
Figure Legend Snippet: Role of ERK1/2, p38, and JNK in BK- and DABK-induced PTGS2 expression in human amnion fibroblasts. (A, B) BK (0.1 μM, 4 h)-induced PTGS2 expression was blocked by the ERK1/2 inhibitor PD98059 (20 µM) (mRNA: n = 4; protein: n = 3) or the p38MAPK inhibitor SB203580 (10 µM) (mRNA: n = 4; protein: n = 3). (C, D) DABK (0.1 μM, 4 h)-induced PTGS2 expression was blocked by the ERK1/2 inhibitor PD98059 (20 µM) (mRNA: n = 3; protein: n = 4) or the p38MAPK inhibitor SB203580 (10 µM) (mRNA: n = 3; protein: n = 4). (E, F) The JNK inhibitor SP600125 (10 µM) failed to block the induction of PTGS2 expression by BK (0.1 μM, 4 h) (mRNA: n = 4; protein: n = 3) or by DABK (0.1 μM, 4 h) (mRNA: n = 5; protein: n = 3). Statistical analysis was performed with one-way ANOVA test followed by Tukey test. Top panels are the representative immunoblots. * p

Techniques Used: Expressing, Blocking Assay, Western Blot

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    Cell Signaling Technology Inc p38
    Phosphorylation of <t>p38,</t> ERK1/2, and JNK by BK and DABK in human amnion fibroblasts. (A–C) Time-dependent effects of BK (0.1 μM) on p38, ERK1/2, and JNK phosphorylation. (D–F) Time-dependent effects of DABK (0.1 μM) on p38, ERK1/2, and JNK phosphorylation. n = 3–4. Statistical analysis was performed with one-way ANOVA test followed by Dunnett’s test. Top panels are the representative immunoblots. * p
    P38, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p38/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p38 - by Bioz Stars, 2022-07
    86/100 stars
      Buy from Supplier

    86
    Synaptic Systems ampars
    Phosphorylation of <t>p38,</t> ERK1/2, and JNK by BK and DABK in human amnion fibroblasts. (A–C) Time-dependent effects of BK (0.1 μM) on p38, ERK1/2, and JNK phosphorylation. (D–F) Time-dependent effects of DABK (0.1 μM) on p38, ERK1/2, and JNK phosphorylation. n = 3–4. Statistical analysis was performed with one-way ANOVA test followed by Dunnett’s test. Top panels are the representative immunoblots. * p
    Ampars, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampars/product/Synaptic Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ampars - by Bioz Stars, 2022-07
    86/100 stars
      Buy from Supplier

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    Phosphorylation of p38, ERK1/2, and JNK by BK and DABK in human amnion fibroblasts. (A–C) Time-dependent effects of BK (0.1 μM) on p38, ERK1/2, and JNK phosphorylation. (D–F) Time-dependent effects of DABK (0.1 μM) on p38, ERK1/2, and JNK phosphorylation. n = 3–4. Statistical analysis was performed with one-way ANOVA test followed by Dunnett’s test. Top panels are the representative immunoblots. * p

    Journal: Frontiers in Endocrinology

    Article Title: The Bradykinin System Contributes to the Regulation of Prostaglandin-Endoperoxide Synthase 2 Expression in Human Amnion Fibroblasts: Implications for Term and Preterm Birth

    doi: 10.3389/fendo.2022.873727

    Figure Lengend Snippet: Phosphorylation of p38, ERK1/2, and JNK by BK and DABK in human amnion fibroblasts. (A–C) Time-dependent effects of BK (0.1 μM) on p38, ERK1/2, and JNK phosphorylation. (D–F) Time-dependent effects of DABK (0.1 μM) on p38, ERK1/2, and JNK phosphorylation. n = 3–4. Statistical analysis was performed with one-way ANOVA test followed by Dunnett’s test. Top panels are the representative immunoblots. * p

    Article Snippet: The primary antibodies used for Western blotting were as follows: p38 (1:500, Cell Signaling, Danvers, MA, USA, #8690), phosphorylated p38 at Thr180/Tyr182 (1:500, Cell Signaling, #4511), ERK1/2 (1:500, Cell Signaling, #4631), phosphorylated ERK1/2 at Thr202/Tyr204 (1:500, Cell Signaling, #4370), JNK (1:1,000, Cell Signaling, #9252), phosphorylated JNK at Thr183/Tyr185 (1:1,000, Cell Signaling, #9255), BDKRB1 (1:100, Santa Cruz, Santa Cruz, CA, USA, #sc-293196), BDKRB2 (1:500, Alomone, Jerusalem BioPark, Israel, #ABR-012), and PTGS2 (1:1,000, Cell Signaling, #12282).

    Techniques: Western Blot

    Role of ERK1/2, p38, and JNK in BK- and DABK-induced PTGS2 expression in human amnion fibroblasts. (A, B) BK (0.1 μM, 4 h)-induced PTGS2 expression was blocked by the ERK1/2 inhibitor PD98059 (20 µM) (mRNA: n = 4; protein: n = 3) or the p38MAPK inhibitor SB203580 (10 µM) (mRNA: n = 4; protein: n = 3). (C, D) DABK (0.1 μM, 4 h)-induced PTGS2 expression was blocked by the ERK1/2 inhibitor PD98059 (20 µM) (mRNA: n = 3; protein: n = 4) or the p38MAPK inhibitor SB203580 (10 µM) (mRNA: n = 3; protein: n = 4). (E, F) The JNK inhibitor SP600125 (10 µM) failed to block the induction of PTGS2 expression by BK (0.1 μM, 4 h) (mRNA: n = 4; protein: n = 3) or by DABK (0.1 μM, 4 h) (mRNA: n = 5; protein: n = 3). Statistical analysis was performed with one-way ANOVA test followed by Tukey test. Top panels are the representative immunoblots. * p

    Journal: Frontiers in Endocrinology

    Article Title: The Bradykinin System Contributes to the Regulation of Prostaglandin-Endoperoxide Synthase 2 Expression in Human Amnion Fibroblasts: Implications for Term and Preterm Birth

    doi: 10.3389/fendo.2022.873727

    Figure Lengend Snippet: Role of ERK1/2, p38, and JNK in BK- and DABK-induced PTGS2 expression in human amnion fibroblasts. (A, B) BK (0.1 μM, 4 h)-induced PTGS2 expression was blocked by the ERK1/2 inhibitor PD98059 (20 µM) (mRNA: n = 4; protein: n = 3) or the p38MAPK inhibitor SB203580 (10 µM) (mRNA: n = 4; protein: n = 3). (C, D) DABK (0.1 μM, 4 h)-induced PTGS2 expression was blocked by the ERK1/2 inhibitor PD98059 (20 µM) (mRNA: n = 3; protein: n = 4) or the p38MAPK inhibitor SB203580 (10 µM) (mRNA: n = 3; protein: n = 4). (E, F) The JNK inhibitor SP600125 (10 µM) failed to block the induction of PTGS2 expression by BK (0.1 μM, 4 h) (mRNA: n = 4; protein: n = 3) or by DABK (0.1 μM, 4 h) (mRNA: n = 5; protein: n = 3). Statistical analysis was performed with one-way ANOVA test followed by Tukey test. Top panels are the representative immunoblots. * p

    Article Snippet: The primary antibodies used for Western blotting were as follows: p38 (1:500, Cell Signaling, Danvers, MA, USA, #8690), phosphorylated p38 at Thr180/Tyr182 (1:500, Cell Signaling, #4511), ERK1/2 (1:500, Cell Signaling, #4631), phosphorylated ERK1/2 at Thr202/Tyr204 (1:500, Cell Signaling, #4370), JNK (1:1,000, Cell Signaling, #9252), phosphorylated JNK at Thr183/Tyr185 (1:1,000, Cell Signaling, #9255), BDKRB1 (1:100, Santa Cruz, Santa Cruz, CA, USA, #sc-293196), BDKRB2 (1:500, Alomone, Jerusalem BioPark, Israel, #ABR-012), and PTGS2 (1:1,000, Cell Signaling, #12282).

    Techniques: Expressing, Blocking Assay, Western Blot