philanthotoxin 74  (Alomone Labs)

Journal: Cells

Article Title: Contribution of the Potassium Channels KV1.3 and KCa3.1 to Smooth Muscle Cell Proliferation in Growing Collateral Arteries

doi: 10.3390/cells9040913

Figure Lengend Snippet: BrdU incorporation and αSM-actin expression in collaterals. ( a , b ) Bar graphs represent the results of quantitative analyses of BrdU + ECs (left panels) and SMCs (right panels) in solvent (control), ( a ) PAP-1 or ( b ) TRAM-34-treated mice at day 7 after induction of arteriogenesis. Data are means ± SEM, n = 3 mice per group. * p

Article Snippet: To block potassium channels, mice were treated either with the selective KV 1.3 channel blocker (5-(4-phenoxybutoxy)psoralen (PAP-1, 40 mg/kg/d, intraperitoneally (i.p), Sigma-Aldrich) [ ], or the selective KCa3.1 channel blocker TRAM-34 (120 mg/kg/d, i.p., Alomone Labs) [ ], dissolved in peanut oil, at doses previously described [ , ].

Techniques: BrdU Incorporation Assay, Expressing, Mouse Assay

Journal: Cells

Article Title: Contribution of the Potassium Channels KV1.3 and KCa3.1 to Smooth Muscle Cell Proliferation in Growing Collateral Arteries

doi: 10.3390/cells9040913

Figure Lengend Snippet: Laser Doppler perfusion measurements. Line plot (left panel) along with corresponding flux images (right panel) of laser Doppler perfusion measurements. Mice were treated with solvent (control), PAP-1, or TRAM-34, respectively, and the perfusion was calculated by right to left (occlusion (occ) to sham) ratio before, immediately after, and at day 3 and 7 after the surgical procedure (left panel). Data are means ± SEM, n = 6 per group. * p

Article Snippet: To block potassium channels, mice were treated either with the selective KV 1.3 channel blocker (5-(4-phenoxybutoxy)psoralen (PAP-1, 40 mg/kg/d, intraperitoneally (i.p), Sigma-Aldrich) [ ], or the selective KCa3.1 channel blocker TRAM-34 (120 mg/kg/d, i.p., Alomone Labs) [ ], dissolved in peanut oil, at doses previously described [ , ].

Techniques: Mouse Assay

Journal: Cells

Article Title: Contribution of the Potassium Channels KV1.3 and KCa3.1 to Smooth Muscle Cell Proliferation in Growing Collateral Arteries

doi: 10.3390/cells9040913

Figure Lengend Snippet: Proliferation assay of mouse primary artery SMCs. Mouse primary artery SMCs were cultured with 10% FCS with or without treatment of different concentrations of the K V 1.3 blocker PAP-1 or the K Ca 3.1 blocker TRAM-34. Cell proliferation was investigated by means of BrdU incorporation. Values are expressed as percentages of the positive control (+), i.e., mouse primary artery SMCs stimulated with 10% FCS. For the negative control (–), mouse primary artery SMCs cultured with 2% FCS. Data are means ± SEM, n > 6 per group. * p

Article Snippet: To block potassium channels, mice were treated either with the selective KV 1.3 channel blocker (5-(4-phenoxybutoxy)psoralen (PAP-1, 40 mg/kg/d, intraperitoneally (i.p), Sigma-Aldrich) [ ], or the selective KCa3.1 channel blocker TRAM-34 (120 mg/kg/d, i.p., Alomone Labs) [ ], dissolved in peanut oil, at doses previously described [ , ].

Techniques: Proliferation Assay, Cell Culture, BrdU Incorporation Assay, Positive Control, Negative Control

Journal: Cells

Article Title: Contribution of the Potassium Channels KV1.3 and KCa3.1 to Smooth Muscle Cell Proliferation in Growing Collateral Arteries

doi: 10.3390/cells9040913

Figure Lengend Snippet: The qRT-PCR results of the expression levels of Fgfr1 , Pdgfrb, and Egr1 in vitro and during arteriogenesis in vivo. ( a , c ) Bar graphs represent the mRNA expression levels of Fgfr1, Pdgfrb, or Egr1 in vitro and ( b , d ) in vivo. In vitro mouse primary artery SMCs were cultured without (control) or with 1 μM PAP-1 or 100 nM TRAM-34, respectively. In vivo the expression level of Fgfr1 , Pdgfrb, and Egr1 were investigated 12 h after induction of arteriogenesis in collateral arteries and are expressed as occlusion (occ) to sham ratio. All qRT-PCR results were normalized to the expression level of the corresponding 18S rRNA. Data are means ± SEM, n = 3 per group. * p

Article Snippet: To block potassium channels, mice were treated either with the selective KV 1.3 channel blocker (5-(4-phenoxybutoxy)psoralen (PAP-1, 40 mg/kg/d, intraperitoneally (i.p), Sigma-Aldrich) [ ], or the selective KCa3.1 channel blocker TRAM-34 (120 mg/kg/d, i.p., Alomone Labs) [ ], dissolved in peanut oil, at doses previously described [ , ].

Techniques: Quantitative RT-PCR, Expressing, In Vitro, In Vivo, Cell Culture

Journal: The European journal of neuroscience

Article Title: Inflammation alters AMPA-stimulated calcium responses in dorsal striatal D2 but not D1 spiny projection neurons

doi: 10.1111/ejn.13711

Figure Lengend Snippet: CP-AMPARs are present in SPNs and LPS selectively decreases AMPA-stimulated [Ca 2+ ] i through CP-AMPARs in D2 SPNs Corticostriatal coronal sections were treated with 125 ng/ml LPS or vehicle for two hours. D2 or D1 SPNs were pretreated via y-tube application with philanthotoxin-74 (300 µM), TTX, and CdCl 2 , before exposure to the AMPA cocktail + philanthotoxin, TTX, and CdCl 2 . A) Peak amplitude of fluorescence response in D2 SPNs (****P

Article Snippet: Studies employed commercially available chemicals as follows: S-AMPA (Abcam, # ), cyclothiazide (Abcam, # ), (R-)CPP (Abcam, # ), TTX (Abcam, # ), philanthotoxin-74 (Alomone Labs, #P-120), and isradipine (Tocris, #2004).

Techniques: Fluorescence