onetaq  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    New England Biolabs onetaq
    Onetaq, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaq - by Bioz Stars, 2022-07
    86/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    New England Biolabs onetaq quick load 2x master mix with standard buffer
    PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.
    Onetaq Quick Load 2x Master Mix With Standard Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq quick load 2x master mix with standard buffer/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaq quick load 2x master mix with standard buffer - by Bioz Stars, 2022-07
    96/100 stars
      Buy from Supplier

    97
    New England Biolabs onetaq 2x master mix with standard buffer
    PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.
    Onetaq 2x Master Mix With Standard Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq 2x master mix with standard buffer/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaq 2x master mix with standard buffer - by Bioz Stars, 2022-07
    97/100 stars
      Buy from Supplier

    95
    New England Biolabs neb onetaq
    Macerprepped DNA is good template for PCR. 1-3, PCR product by <t>NEB</t> Q5® HiFi polymerase. 4-6, PCR product by NEB <t>Onetaq</t> polymerase. I, 4, Miraprepped DNA. 2, 5, Macerprepped DNA with Solution 3 process. 3, 6, Macerprepped DNA with Solution N3 process. M, marker.
    Neb Onetaq, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neb onetaq/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neb onetaq - by Bioz Stars, 2022-07
    95/100 stars
      Buy from Supplier

    Image Search Results


    PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.

    Journal: bioRxiv

    Article Title: Akaby - cell-free protein expression system for linear templates

    doi: 10.1101/2021.11.03.467179

    Figure Lengend Snippet: PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.

    Article Snippet: 10 μl total volume of colony PCR was performed with 0.4 μM of locus-(Primer 1 and 4) and insert-(Primer 2 and 3) specific primer pairs, 1X OneTaq® Quick-Load® 2X Master Mix with Standard Buffer (New England BioLabs Inc., M0486L), and 2 μl of water suspended colony.

    Techniques: Polymerase Chain Reaction, Amplification, Construct, Plasmid Preparation, Sequencing

    PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.

    Journal: bioRxiv

    Article Title: Akaby - cell-free protein expression system for linear templates

    doi: 10.1101/2021.11.03.467179

    Figure Lengend Snippet: PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.

    Article Snippet: The oligo was PCR amplified with OneTaq® 2X Master Mix with Standard Buffer (New England BioLabs Inc., M0482L), followed by agarose gel purification with GenCatch Advanced Gel Extraction Kit (Epoch Life Science, Inc., No. 2260050).

    Techniques: Polymerase Chain Reaction, Amplification, Construct, Plasmid Preparation, Sequencing

    Macerprepped DNA is good template for PCR. 1-3, PCR product by NEB Q5® HiFi polymerase. 4-6, PCR product by NEB Onetaq polymerase. I, 4, Miraprepped DNA. 2, 5, Macerprepped DNA with Solution 3 process. 3, 6, Macerprepped DNA with Solution N3 process. M, marker.

    Journal: bioRxiv

    Article Title: The Macerprep: a minimalist kit- and enzyme-free high-yield miniprep utilising alkaline lysis and alkaline hydrolysis principles

    doi: 10.1101/2020.08.13.249607

    Figure Lengend Snippet: Macerprepped DNA is good template for PCR. 1-3, PCR product by NEB Q5® HiFi polymerase. 4-6, PCR product by NEB Onetaq polymerase. I, 4, Miraprepped DNA. 2, 5, Macerprepped DNA with Solution 3 process. 3, 6, Macerprepped DNA with Solution N3 process. M, marker.

    Article Snippet: According to the NEB calculator (NEB, online tool), Tm for NEB Q5 and NEB Onetaq was determined to be 52°C and 61°C.

    Techniques: Polymerase Chain Reaction, Marker

    PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.

    Journal: bioRxiv

    Article Title: Akaby - cell-free protein expression system for linear templates

    doi: 10.1101/2021.11.03.467179

    Figure Lengend Snippet: PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.

    Article Snippet: The quantitative PCR reaction mix was prepared by mixing 2 μl of complementary DNA from the reverse transcription with 2 μl of 10 μM forward and reverse primers, 11.25 μl OneTaq Hot Start 2X Master Mix with Standard Buffer (Catalog No. M0484, New England BioLabs Inc.), 1.25 μl Chai Green Dye 20X (Catalog No. R01200, Chai Bio), and 7.5 μl of nuclease-free water.

    Techniques: Polymerase Chain Reaction, Amplification, Construct, Plasmid Preparation, Sequencing