iaa  (Worthington Biochemical)


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  • 94
    Name:
    Trypsin Modified Sequencing Grade
    Description:
    Trypsin treated with L tosylamido 2 phenyl ethyl chloromethyl ketone to inhibit contaminating chymotryptic activity chemically modified to promote stability and further purified to remove autolysis fragments resulting in a highly stable trypsin product resistant to autolysis while retaining specificity
    Catalog Number:
    LS02120
    Price:
    78
    Source:
    Bovine Pancreas
    Size:
    4x25 ug
    Cas Number:
    9002.07.7
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    Structured Review

    Worthington Biochemical iaa
    Trypsin treated with L tosylamido 2 phenyl ethyl chloromethyl ketone to inhibit contaminating chymotryptic activity chemically modified to promote stability and further purified to remove autolysis fragments resulting in a highly stable trypsin product resistant to autolysis while retaining specificity
    https://www.bioz.com/result/iaa/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iaa - by Bioz Stars, 2021-10
    94/100 stars

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    Related Articles

    Staining:

    Article Title: SIRT3 consolidates heterochromatin and counteracts senescence
    Article Snippet: .. Protein bands stained by Coomassie blue were cut from the SDS-PAGE gel, subjected to decoloration and dehydration, and then digested with sequencing-grade Trypsin (Worthington). ..

    SDS Page:

    Article Title: SIRT3 consolidates heterochromatin and counteracts senescence
    Article Snippet: .. Protein bands stained by Coomassie blue were cut from the SDS-PAGE gel, subjected to decoloration and dehydration, and then digested with sequencing-grade Trypsin (Worthington). ..

    Sequencing:

    Article Title: SIRT3 consolidates heterochromatin and counteracts senescence
    Article Snippet: .. Protein bands stained by Coomassie blue were cut from the SDS-PAGE gel, subjected to decoloration and dehydration, and then digested with sequencing-grade Trypsin (Worthington). ..

    Article Title: ZKSCAN3 counteracts cellular senescence by stabilizing heterochromatin
    Article Snippet: .. Proteins were then digested into peptides by sequencing grade trypsin (Worthington) overnight at 37°C. ..

    Article Title: Impact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells
    Article Snippet: .. Following pooling of samples, sequencing grade trypsin (Worthington) was added for digestion in a trypsin to protein mass ratio of 1:50 and the sample was incubated for 2 h at 50 °C. ..

    Article Title: A Universal Stress Protein That Controls Bacterial Stress Survival in Micrococcus luteus
    Article Snippet: .. Precipitated protein pellets were resuspended in 80 μl of 25 mM Tris-HCl (pH 7.4), and sequencing-grade trypsin (250 ng; Worthington Biochemicals) was added. ..

    Article Title: Identification of Novel Natural Substrates of Fibroblast Activation Protein-alpha by Differential Degradomics and Proteomics
    Article Snippet: .. Gel pieces were digested with sequencing grade trypsin (Worthington) at pH 7.5, overnight at 37 °C. ..

    Article Title: Partitioning of MLX-Family Transcription Factors to Lipid Droplets Regulates Metabolic Gene Expression
    Article Snippet: .. Protein mixtures were diluted in 1:6 ratio (v/v) using ultrapure water prior to digestion using sequencing grade trypsin (LS02120, Worthington Biochemical) for 16 hours at 37°C. ..

    Article Title: Lipid droplet assembly factor-1 and seipin form a lipid droplet assembly complex
    Article Snippet: .. Alkylated proteins were diluted in 1:6 ratio (v/v) in ultrapure water prior to digestion using sequencing-grade trypsin (Worthington Biochemical Corp) at 37°C for 16 hrs. ..

    Article Title: Comprehensive analysis of protein digestion using six trypsins reveals the origin of trypsin as a significant source of variability in proteomics
    Article Snippet: .. Six proteomics grade trypsins were used in the study (M1: G Biosciences #786-245, mass spectrometry grade; M2: Princeton Separations #EN-151, sequencing grade; M3: Promega #V5111, sequencing grade; M4: G Biosciences #786-245B, mass spectrometry grade; M5: Roche Applied Science #11418025001, sequencing grade; M6: Worthington Biochemical Corp. #LS02120). ..

    Incubation:

    Article Title: Impact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells
    Article Snippet: .. Following pooling of samples, sequencing grade trypsin (Worthington) was added for digestion in a trypsin to protein mass ratio of 1:50 and the sample was incubated for 2 h at 50 °C. ..

    Mass Spectrometry:

    Article Title: Comprehensive analysis of protein digestion using six trypsins reveals the origin of trypsin as a significant source of variability in proteomics
    Article Snippet: .. Six proteomics grade trypsins were used in the study (M1: G Biosciences #786-245, mass spectrometry grade; M2: Princeton Separations #EN-151, sequencing grade; M3: Promega #V5111, sequencing grade; M4: G Biosciences #786-245B, mass spectrometry grade; M5: Roche Applied Science #11418025001, sequencing grade; M6: Worthington Biochemical Corp. #LS02120). ..

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