iaa  (Worthington Biochemical)


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  • 99
    Name:
    Trypsin
    Description:
    Supplied as dialyzed and lyophilized powder
    Catalog Number:
    LS003702
    Price:
    23
    Source:
    Bovine Pancreas
    Size:
    100 mg
    Cas Number:
    9002.07.7
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    Structured Review

    Worthington Biochemical iaa
    Supplied as dialyzed and lyophilized powder
    https://www.bioz.com/result/iaa/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iaa - by Bioz Stars, 2021-04
    99/100 stars

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    Related Articles

    Purification:

    Article Title: Differential Induction of Type I and Type III Interferons by Swine and Human Origin H1N1 Influenza A Viruses in Porcine Airway Epithelial Cells
    Article Snippet: The swine influenza viruses; A/Sw/Illinois/2008 (H1N1 IL/08), A/Sw/Iowa/2004 (H1N1 IA/04), and A (H3N2) variant virus (H3N2v), and the human pandemic influenza A/California/04/2009 (pH1N1 CA/09) were obtained from the University of Minnesota Veterinary Diagnostic Laboratory (St Paul, MN). .. Viruses were propagated in MDCK cells in DMEM containing 0.5 μg/ml TPCK-trypsin (Worthington Biochemical Corporation, Lakewood, NJ) and purified from the clarified cell culture supernatants by ultracentrifugation through a 30% (w/v) sucrose cushion and stored in aliquots at -80o C. Culture supernatant from un-infected MDCK cells were processed similarly to use for mock infection. .. Isolation of primary pAECs Primary pAECs were isolated from three healthy neonatal pigs as described previously [ ] with slight modifications.

    Article Title: The analysis of macromolecular interactions by sedimentation equilibrium
    Article Snippet: .. Purified α-chymotrypsin (Cat. No. 1475) and soybean trypsin inhibitor (STI) (Cat. No. 3570) were purchased from Worthington Biochemical Corporation. .. Approximately 21 mg of each were dissolved in 0.2M sodium chloride and 0.05M sodium phosphate (pH = 6.7) and further purified by size exclusion chromatography on a HiPrep 16/60 Sephacryl S-200 HR column (Cat. No. 17-1166-01, GE Healthcare) at 0.5 mL/min in the same buffer.

    Cell Culture:

    Article Title: Differential Induction of Type I and Type III Interferons by Swine and Human Origin H1N1 Influenza A Viruses in Porcine Airway Epithelial Cells
    Article Snippet: The swine influenza viruses; A/Sw/Illinois/2008 (H1N1 IL/08), A/Sw/Iowa/2004 (H1N1 IA/04), and A (H3N2) variant virus (H3N2v), and the human pandemic influenza A/California/04/2009 (pH1N1 CA/09) were obtained from the University of Minnesota Veterinary Diagnostic Laboratory (St Paul, MN). .. Viruses were propagated in MDCK cells in DMEM containing 0.5 μg/ml TPCK-trypsin (Worthington Biochemical Corporation, Lakewood, NJ) and purified from the clarified cell culture supernatants by ultracentrifugation through a 30% (w/v) sucrose cushion and stored in aliquots at -80o C. Culture supernatant from un-infected MDCK cells were processed similarly to use for mock infection. .. Isolation of primary pAECs Primary pAECs were isolated from three healthy neonatal pigs as described previously [ ] with slight modifications.

    Infection:

    Article Title: Differential Induction of Type I and Type III Interferons by Swine and Human Origin H1N1 Influenza A Viruses in Porcine Airway Epithelial Cells
    Article Snippet: The swine influenza viruses; A/Sw/Illinois/2008 (H1N1 IL/08), A/Sw/Iowa/2004 (H1N1 IA/04), and A (H3N2) variant virus (H3N2v), and the human pandemic influenza A/California/04/2009 (pH1N1 CA/09) were obtained from the University of Minnesota Veterinary Diagnostic Laboratory (St Paul, MN). .. Viruses were propagated in MDCK cells in DMEM containing 0.5 μg/ml TPCK-trypsin (Worthington Biochemical Corporation, Lakewood, NJ) and purified from the clarified cell culture supernatants by ultracentrifugation through a 30% (w/v) sucrose cushion and stored in aliquots at -80o C. Culture supernatant from un-infected MDCK cells were processed similarly to use for mock infection. .. Isolation of primary pAECs Primary pAECs were isolated from three healthy neonatal pigs as described previously [ ] with slight modifications.

    Article Title: Unique Type I Interferon Responses Determine the Functional Fate of Migratory Lung Dendritic Cells during Influenza Virus Infection
    Article Snippet: Supporting Information Migratory DCs can transfer infectious virus to uninfected cells in the absence of TPCK-trypsin. .. A. MLN-DCs (gate V, ) from PR8 or WSN infected mice (day 3) were co-cultured with MDCK cells in the presence or absence of TPCK-trypsin for 2 days. .. Virus replication was assessed by HRP-based immunostaining of MDCK cells with a chicken polyclonal antibody to influenza virus.

    other:

    Article Title: Exploring protein interfaces with a general photochemical reagent
    Article Snippet: Complete enzymatic digestion of these carbamidomethylated samples with TPCK trypsin was achieved in NH4 CO3 H (0.1 M at pH 8.0), after 12–24 h at 37°C using a 2% (w/w) enzyme:substrate ratio.

    Article Title: Unique Type I Interferon Responses Determine the Functional Fate of Migratory Lung Dendritic Cells during Influenza Virus Infection
    Article Snippet: Supporting Information Migratory DCs can transfer infectious virus to uninfected cells in the absence of TPCK-trypsin.

    Incubation:

    Article Title: Conformational Changes in the Spike Glycoprotein of Murine Coronavirus Are Induced at 37?C either by Soluble Murine CEACAM1 Receptors or by pH 8
    Article Snippet: Incubation of MHV-A59 virions at pH 6.5 and 37°C with soluble murine receptor glycoproteins CEACAM1a[1-4] (Fig. ) or the corresponding two-domain isoform smCEACAM1a[1,4] (data not shown) followed by trypsin-TPCK digestion at 4°C resulted in degradation of the S2 protein but not of the S1 protein. .. Incubation of MHV-A59 virions with smCEACAM1a[1-4] receptor glycoprotein at 4°C did not make the S2 protein susceptible to degradation by trypsin-TPCK at 4°C (Fig. ). ..

    Mouse Assay:

    Article Title: Unique Type I Interferon Responses Determine the Functional Fate of Migratory Lung Dendritic Cells during Influenza Virus Infection
    Article Snippet: Supporting Information Migratory DCs can transfer infectious virus to uninfected cells in the absence of TPCK-trypsin. .. A. MLN-DCs (gate V, ) from PR8 or WSN infected mice (day 3) were co-cultured with MDCK cells in the presence or absence of TPCK-trypsin for 2 days. .. Virus replication was assessed by HRP-based immunostaining of MDCK cells with a chicken polyclonal antibody to influenza virus.

    Article Title: Unique Type I Interferon Responses Determine the Functional Fate of Migratory Lung Dendritic Cells during Influenza Virus Infection
    Article Snippet: Control wells were stained with chicken normal serum followed by HRP-based immunostaining. .. B. Sorted CD103+ DCs and CD11bhigh DCs from PR8 or WSN-infected mice were co-cultured with MDCK cells in the presence or absence of TPCK-trypsin and the culture supernatants were assayed for infectious virus particles at day 2 by hemmaglutination of RBCs. (PDF) Click here for additional data file. .. Sorting strategy to isolate LN-resident CD8α+ DCs during influenza virus infection.

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