iaa  (Worthington Biochemical)


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  • 94
    Name:
    Trypsin Modified Sequencing Grade
    Description:
    Trypsin treated with L tosylamido 2 phenyl ethyl chloromethyl ketone to inhibit contaminating chymotryptic activity chemically modified to promote stability and further purified to remove autolysis fragments resulting in a highly stable trypsin product resistant to autolysis while retaining specificity
    Catalog Number:
    LS02120
    Price:
    78
    Source:
    Bovine Pancreas
    Size:
    4x25 ug
    Cas Number:
    9002.07.7
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    Structured Review

    Worthington Biochemical iaa
    Trypsin treated with L tosylamido 2 phenyl ethyl chloromethyl ketone to inhibit contaminating chymotryptic activity chemically modified to promote stability and further purified to remove autolysis fragments resulting in a highly stable trypsin product resistant to autolysis while retaining specificity
    https://www.bioz.com/result/iaa/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iaa - by Bioz Stars, 2021-07
    94/100 stars

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    Related Articles

    Staining:

    Article Title: SIRT3 consolidates heterochromatin and counteracts senescence
    Article Snippet: Briefly, eluted proteins from the co-IP assay were separated using 12% SDS-PAGE gel and then stained with Coomassie blue. .. Protein bands stained by Coomassie blue were cut from the SDS-PAGE gel, subjected to decoloration and dehydration, and then digested with sequencing-grade Trypsin (Worthington). ..

    SDS Page:

    Article Title: SIRT3 consolidates heterochromatin and counteracts senescence
    Article Snippet: Briefly, eluted proteins from the co-IP assay were separated using 12% SDS-PAGE gel and then stained with Coomassie blue. .. Protein bands stained by Coomassie blue were cut from the SDS-PAGE gel, subjected to decoloration and dehydration, and then digested with sequencing-grade Trypsin (Worthington). ..

    Sequencing:

    Article Title: SIRT3 consolidates heterochromatin and counteracts senescence
    Article Snippet: Briefly, eluted proteins from the co-IP assay were separated using 12% SDS-PAGE gel and then stained with Coomassie blue. .. Protein bands stained by Coomassie blue were cut from the SDS-PAGE gel, subjected to decoloration and dehydration, and then digested with sequencing-grade Trypsin (Worthington). ..

    Article Title: ZKSCAN3 counteracts cellular senescence by stabilizing heterochromatin
    Article Snippet: .. Proteins were then digested into peptides by sequencing grade trypsin (Worthington) overnight at 37°C. .. The resultant peptides were homogenized in 0.1% formic acid and separated by the online Easy-nLC 1000 system (Thermo Fisher Scientific) with a C18 reverse-phase column.

    Article Title: Impact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells
    Article Snippet: Subsequently, DNA was sheared using ultra-sonication for 10 × 30 s. Iodoacetamide (20 mM) was added for alkylation of cysteines and incubated for 30 min at room temperature in the dark. α- and ε-amines were labeled for 18 h at room temperature by adding tandem mass tag (TMT) 11 plex reagents (Thermo), which were resuspended in acetonitrile with a protein to TMT reagents mass ratio of 1:8. .. Following pooling of samples, sequencing grade trypsin (Worthington) was added for digestion in a trypsin to protein mass ratio of 1:50 and the sample was incubated for 2 h at 50 °C. .. Internal peptides were then coupled to an aldehyde-functionalized polymer in the presence of 50 mM NaBH3CN and removed from TMT-blocked N-terminal peptides by ultrafiltration through a 10 kDa MWCO filter (Millipore, Burlington, MA, USA).

    Article Title: A Universal Stress Protein That Controls Bacterial Stress Survival in Micrococcus luteus
    Article Snippet: Samples were reduced by 10 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) at 37°C for 30 min, alkylated by 10 mM iodoacetamide at room temperature in the dark, and stored at −80°C or subjected to quantitative protein chloroform-methanol precipitation as previously described ( , ). (ii) Trypsin digestion. .. Precipitated protein pellets were resuspended in 80 μl of 25 mM Tris-HCl (pH 7.4), and sequencing-grade trypsin (250 ng; Worthington Biochemicals) was added. .. The reaction mixture was incubated for 18 to 24 h at 37°C before quenching by addition of 10 μl of 10% formic acid.

    Article Title: Identification of Novel Natural Substrates of Fibroblast Activation Protein-alpha by Differential Degradomics and Proteomics
    Article Snippet: .. Gel pieces were digested with sequencing grade trypsin (Worthington) at pH 7.5, overnight at 37 °C. .. After digestion, peptides were eluted from the gel with 100% ethanol, samples were then concentrated using a Speedvac concentrator (model 5301, Eppendorf, Hamburg, Germany) and desalted using a reversed phase C18 column (GRACE-VYDAC, Deerfield, IL).

    Article Title: Partitioning of MLX-Family Transcription Factors to Lipid Droplets Regulates Metabolic Gene Expression
    Article Snippet: .. Protein mixtures were diluted in 1:6 ratio (v/v) using ultrapure water prior to digestion using sequencing grade trypsin (LS02120, Worthington Biochemical) for 16 hours at 37°C. ..

    Article Title: Lipid droplet assembly factor-1 and seipin form a lipid droplet assembly complex
    Article Snippet: Resin containing purified immunoprecipitates was incubated in 200 mM HEPES (4-(hydroxyethyl)-1-piperazineethanesulfonic acid), pH 7.5 containing 5 mM dithiothreitol (Sigma-Aldrich) at 37°C for 1 hr, followed by alkylation of cysteine residues using 15 mM iodoacetamide (Sigma-Aldrich) in the dark at room temperature for 1 hour. .. Alkylated proteins were diluted in 1:6 ratio (v/v) in ultrapure water prior to digestion using sequencing-grade trypsin (Worthington Biochemical Corp) at 37°C for 16 hrs. .. SUM159 cells stably expressing seipin WT-EGFP, seipin ΔHH-EGFP, or seipin TMD(FIT2)-EGFP (at a safe-harbor locus) from 3 × 15 cm dishes at 90% confluence were harvested in lysis buffer without detergent (50 mM Tris-HCl/pH 8.0, 150 mM NaCl) supplemented with protease inhibitor cocktail (Roche) and homogenized with a Dounce homogenizer ~ 30 times on ice.

    Article Title: Comprehensive analysis of protein digestion using six trypsins reveals the origin of trypsin as a significant source of variability in proteomics
    Article Snippet: .. Six proteomics grade trypsins were used in the study (M1: G Biosciences #786-245, mass spectrometry grade; M2: Princeton Separations #EN-151, sequencing grade; M3: Promega #V5111, sequencing grade; M4: G Biosciences #786-245B, mass spectrometry grade; M5: Roche Applied Science #11418025001, sequencing grade; M6: Worthington Biochemical Corp. #LS02120). .. These were all TPCK treated to reduce the chymotryptic activity and methylated for resistance to autolysis.

    Incubation:

    Article Title: Impact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells
    Article Snippet: Subsequently, DNA was sheared using ultra-sonication for 10 × 30 s. Iodoacetamide (20 mM) was added for alkylation of cysteines and incubated for 30 min at room temperature in the dark. α- and ε-amines were labeled for 18 h at room temperature by adding tandem mass tag (TMT) 11 plex reagents (Thermo), which were resuspended in acetonitrile with a protein to TMT reagents mass ratio of 1:8. .. Following pooling of samples, sequencing grade trypsin (Worthington) was added for digestion in a trypsin to protein mass ratio of 1:50 and the sample was incubated for 2 h at 50 °C. .. Internal peptides were then coupled to an aldehyde-functionalized polymer in the presence of 50 mM NaBH3CN and removed from TMT-blocked N-terminal peptides by ultrafiltration through a 10 kDa MWCO filter (Millipore, Burlington, MA, USA).

    Mass Spectrometry:

    Article Title: Comprehensive analysis of protein digestion using six trypsins reveals the origin of trypsin as a significant source of variability in proteomics
    Article Snippet: .. Six proteomics grade trypsins were used in the study (M1: G Biosciences #786-245, mass spectrometry grade; M2: Princeton Separations #EN-151, sequencing grade; M3: Promega #V5111, sequencing grade; M4: G Biosciences #786-245B, mass spectrometry grade; M5: Roche Applied Science #11418025001, sequencing grade; M6: Worthington Biochemical Corp. #LS02120). .. These were all TPCK treated to reduce the chymotryptic activity and methylated for resistance to autolysis.

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    Worthington Biochemical oxide remover
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    https://www.bioz.com/result/oxide remover/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
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