cardiomyocyte isolation kit (Worthington Biochemical)


Name:
Neonatal Cardiomyocyte Isolation System
Description:
Kit for performing five separate tissue dissociations each containing up to twelve hearts Contains single use vials of purified collagenase and trypsin CMF HBSS Leibovitz L 15 media and Falcon cell strainers along with a detailed protocol The kit is use tested by Worthington to assure performance
Catalog Number:
lk003300
Price:
256
Size:
1 kt
Cas Number:
see components
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Structured Review
![Percentage (mean + SD) of ( a ) GM1, ( b ) [ 3 H]OA, and ( c ) [ 14 C]DHA in sucrose-gradient fractions of neonatal <t>cardiomyocytes.</t> Cells were incubated at 4°C in the presence of 1% Triton X-100, homogenized, and placed on a 5–40% sucrose gradient. After ultracentrifugation, fractions were collected in 1-mL increments from the top to bottom of the centrifuge tubes and analyzed (Materials and Methods). Values represent a minimum of three separate experiments.](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1304580/bin/biophysj00044552F08_LW.jpg)
Kit for performing five separate tissue dissociations each containing up to twelve hearts Contains single use vials of purified collagenase and trypsin CMF HBSS Leibovitz L 15 media and Falcon cell strainers along with a detailed protocol The kit is use tested by Worthington to assure performance
https://www.bioz.com/result/cardiomyocyte isolation kit/product/Worthington Biochemical
Average 97 stars, based on 2350 article reviews
Price from $9.99 to $1999.99
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Images
1) Product Images from "Oleic and Docosahexaenoic Acid Differentially Phase Separate from Lipid Raft Molecules: A Comparative NMR, DSC, AFM, and Detergent Extraction Study"
Article Title: Oleic and Docosahexaenoic Acid Differentially Phase Separate from Lipid Raft Molecules: A Comparative NMR, DSC, AFM, and Detergent Extraction Study
Journal: Biophysical Journal
doi: 10.1529/biophysj.104.044552
![... [ 14 C]DHA in sucrose-gradient fractions of neonatal cardiomyocytes. Cells were incubated at 4°C in the presence ... Percentage (mean + SD) of ( a ) GM1, ( b ) [ 3 H]OA, and ( c ) [ 14 C]DHA in sucrose-gradient fractions of neonatal cardiomyocytes. Cells were incubated at 4°C in the presence of 1% Triton X-100, homogenized, and placed on a 5–40% sucrose gradient. After ultracentrifugation, fractions were collected in 1-mL increments from the top to bottom of the centrifuge tubes and analyzed (Materials and Methods). Values represent a minimum of three separate experiments.](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1304580/bin/biophysj00044552F08_LW.jpg)
Figure Legend Snippet: Percentage (mean + SD) of ( a ) GM1, ( b ) [ 3 H]OA, and ( c ) [ 14 C]DHA in sucrose-gradient fractions of neonatal cardiomyocytes. Cells were incubated at 4°C in the presence of 1% Triton X-100, homogenized, and placed on a 5–40% sucrose gradient. After ultracentrifugation, fractions were collected in 1-mL increments from the top to bottom of the centrifuge tubes and analyzed (Materials and Methods). Values represent a minimum of three separate experiments.
Techniques Used: Incubation
2) Product Images from "Suppressive Effects of Insulin on Tumor Necrosis Factor–Dependent Early Osteoarthritic Changes Associated With Obesity and Type 2 Diabetes Mellitus"
Article Title: Suppressive Effects of Insulin on Tumor Necrosis Factor–Dependent Early Osteoarthritic Changes Associated With Obesity and Type 2 Diabetes Mellitus
Journal: Arthritis & Rheumatology (Hoboken, N.j.)
doi: 10.1002/art.39561

Figure Legend Snippet: Insulin receptors (IRs) are expressed in mouse and human synovium and are functional in human fibroblast‐like synoviocytes (FLS). A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.
Techniques Used: Functional Assay, Immunohistochemistry, Staining, Negative Control, Expressing, Isolation, Western Blot
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