cardiomyocyte isolation kit  (Worthington Biochemical)


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    Name:
    Neonatal Cardiomyocyte Isolation System
    Description:
    Kit for performing five separate tissue dissociations each containing up to twelve hearts Contains single use vials of purified collagenase and trypsin CMF HBSS Leibovitz L 15 media and Falcon cell strainers along with a detailed protocol The kit is use tested by Worthington to assure performance
    Catalog Number:
    lk003300
    Price:
    256
    Size:
    1 kt
    Cas Number:
    see components
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    Structured Review

    Worthington Biochemical cardiomyocyte isolation kit
    Percentage (mean + SD) of ( a ) GM1, ( b ) [ 3 H]OA, and ( c ) [ 14 C]DHA in sucrose-gradient fractions of neonatal <t>cardiomyocytes.</t> Cells were incubated at 4°C in the presence of 1% Triton X-100, homogenized, and placed on a 5–40% sucrose gradient. After ultracentrifugation, fractions were collected in 1-mL increments from the top to bottom of the centrifuge tubes and analyzed (Materials and Methods). Values represent a minimum of three separate experiments.
    Kit for performing five separate tissue dissociations each containing up to twelve hearts Contains single use vials of purified collagenase and trypsin CMF HBSS Leibovitz L 15 media and Falcon cell strainers along with a detailed protocol The kit is use tested by Worthington to assure performance
    https://www.bioz.com/result/cardiomyocyte isolation kit/product/Worthington Biochemical
    Average 98 stars, based on 2351 article reviews
    Price from $9.99 to $1999.99
    cardiomyocyte isolation kit - by Bioz Stars, 2020-08
    98/100 stars

    Images

    1) Product Images from "Oleic and Docosahexaenoic Acid Differentially Phase Separate from Lipid Raft Molecules: A Comparative NMR, DSC, AFM, and Detergent Extraction Study"

    Article Title: Oleic and Docosahexaenoic Acid Differentially Phase Separate from Lipid Raft Molecules: A Comparative NMR, DSC, AFM, and Detergent Extraction Study

    Journal: Biophysical Journal

    doi: 10.1529/biophysj.104.044552

    Percentage (mean + SD) of ( a ) GM1, ( b ) [ 3 H]OA, and ( c ) [ 14 C]DHA in sucrose-gradient fractions of neonatal cardiomyocytes. Cells were incubated at 4°C in the presence of 1% Triton X-100, homogenized, and placed on a 5–40% sucrose gradient. After ultracentrifugation, fractions were collected in 1-mL increments from the top to bottom of the centrifuge tubes and analyzed (Materials and Methods). Values represent a minimum of three separate experiments.
    Figure Legend Snippet: Percentage (mean + SD) of ( a ) GM1, ( b ) [ 3 H]OA, and ( c ) [ 14 C]DHA in sucrose-gradient fractions of neonatal cardiomyocytes. Cells were incubated at 4°C in the presence of 1% Triton X-100, homogenized, and placed on a 5–40% sucrose gradient. After ultracentrifugation, fractions were collected in 1-mL increments from the top to bottom of the centrifuge tubes and analyzed (Materials and Methods). Values represent a minimum of three separate experiments.

    Techniques Used: Incubation

    2) Product Images from "Suppressive Effects of Insulin on Tumor Necrosis Factor–Dependent Early Osteoarthritic Changes Associated With Obesity and Type 2 Diabetes Mellitus"

    Article Title: Suppressive Effects of Insulin on Tumor Necrosis Factor–Dependent Early Osteoarthritic Changes Associated With Obesity and Type 2 Diabetes Mellitus

    Journal: Arthritis & Rheumatology (Hoboken, N.j.)

    doi: 10.1002/art.39561

    Insulin receptors (IRs) are expressed in mouse and human synovium and are functional in human fibroblast‐like synoviocytes (FLS). A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.
    Figure Legend Snippet: Insulin receptors (IRs) are expressed in mouse and human synovium and are functional in human fibroblast‐like synoviocytes (FLS). A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.

    Techniques Used: Functional Assay, Immunohistochemistry, Staining, Negative Control, Expressing, Isolation, Western Blot

    Related Articles

    Patch Clamp:

    Article Title: Stilbene derivative as a photosensitive compound to control the excitability of neonatal rat cardiomyocytes
    Article Snippet: .. Preparation of neonatal rat ventricular cardiomyocyte (NRVM) for patch clamp for optical mapping Hearts were isolated from neonatal 1–2-day-old Wistar rats using a two-day protocol of Neonatal Cardiomyocyte Isolation System of Worthington Biochemical Corporation [ ] with some modifications. ..

    Isolation:

    Article Title: Three-Dimensional Culture Alters Primary Cardiac Cell Phenotype
    Article Snippet: .. NRCVCs were isolated using Neonatal Cardiomyocyte Isolation System kits (Worthington Biochemical, Lakewood, NJ) as in previous studies by our group. .. Viable cells were enumerated by Trypan-blue exclusion (Invitrogen, Carlsbad, CA).

    Article Title: Single Cell Transcriptomics Reconstructs Fate Conversion from Fibroblast to Cardiomyocyte
    Article Snippet: .. Neonatal CMs were isolated using the neonatal cardiomyocytes isolation system (Worthington Biochemical Corporation) except that all enzymes were used at a ¼ of the recommended concentration to increase cell viability. .. After a 1.5 hour pre-plating on uncoated surface to remove attached nonmyocytes, the unattached CMs were collected in TRIzol ( > 80% viability by Trypan blue staining).

    Article Title: Control of histone H3 phosphorylation by CaMKIIδ in response to haemodynamic cardiac stress
    Article Snippet: .. Primary neonatal rat cardiomyocytes isolated using the Worthington Neonatal CardioMyocytes System (Worthington, USA) were co-transfected with a GFP-tagged plasmid and siControl (siCt) or siRNA against 14–3–3 (si14–3–3) from Dharmacon (Thermo Scientific, USA) using lipofectamine (Invitrogen). .. 24 h post-transfection, total cell lysates were prepared and analysed by western blotting or immunofluorescence.

    Article Title: Human Myocardial Pericytes: Multipotent Mesodermal Precursors Exhibiting Cardiac Specificity
    Article Snippet: .. Rat neonatal cardiomyocytes were isolated from anaesthetized 3-day-old rat pups using the Worthington rat neonatal cardiomyocyte isolation system per the manufacturer’s protocol (Worthington Biochemical Corp). .. Pericyte/cardiomyocyte co-culture protocol was adopted and modified from previous reports ( , ).

    Article Title: Muscle ring finger protein-1 inhibits PKC? activation and prevents cardiomyocyte hypertrophy
    Article Snippet: .. NRVM were isolated using the neonatal cardiomyocyte isolation kit (Worthington) and were plated on laminin. ..

    Article Title: An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses
    Article Snippet: .. Primary cardiomyocytes were isolated from newborn Dahl salt-sensitive rats using the Neonatal Cardiomyocytes Isolation System (Worthington, Lakewood, NJ) according to the manufacturer’s instruction. ..

    Article Title: Pivotal role of cardiac lineage protein-1 (CLP-1) in transcriptional elongation factor P-TEFb complex formation in cardiac hypertrophy
    Article Snippet: .. Cardiac cells of 2–4-day-old WKY rats were isolated using a neonatal cardiomyocyte isolation system (Worthington Biochemical Corp, Lakewood, New Jersey, USA). .. After pre-plating to reduce contaminating non-muscle cells, cardiomyocytes were plated on Bioflex collagen 6-well plates (Flexcell, McKeesport, Pennsylvania, USA) and cultured in DMEM/F-12 (1:1) media supplemented with 10% fetal bovine serum for approximately 1.5 days prior to switching to serum-free medium.

    Article Title: Stilbene derivative as a photosensitive compound to control the excitability of neonatal rat cardiomyocytes
    Article Snippet: .. Preparation of neonatal rat ventricular cardiomyocyte (NRVM) for patch clamp for optical mapping Hearts were isolated from neonatal 1–2-day-old Wistar rats using a two-day protocol of Neonatal Cardiomyocyte Isolation System of Worthington Biochemical Corporation [ ] with some modifications. ..

    Plasmid Preparation:

    Article Title: Control of histone H3 phosphorylation by CaMKIIδ in response to haemodynamic cardiac stress
    Article Snippet: .. Primary neonatal rat cardiomyocytes isolated using the Worthington Neonatal CardioMyocytes System (Worthington, USA) were co-transfected with a GFP-tagged plasmid and siControl (siCt) or siRNA against 14–3–3 (si14–3–3) from Dharmacon (Thermo Scientific, USA) using lipofectamine (Invitrogen). .. 24 h post-transfection, total cell lysates were prepared and analysed by western blotting or immunofluorescence.

    Concentration Assay:

    Article Title: Single Cell Transcriptomics Reconstructs Fate Conversion from Fibroblast to Cardiomyocyte
    Article Snippet: .. Neonatal CMs were isolated using the neonatal cardiomyocytes isolation system (Worthington Biochemical Corporation) except that all enzymes were used at a ¼ of the recommended concentration to increase cell viability. .. After a 1.5 hour pre-plating on uncoated surface to remove attached nonmyocytes, the unattached CMs were collected in TRIzol ( > 80% viability by Trypan blue staining).

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    Worthington Biochemical human oa fls human synovium
    Insulin receptors (IRs) are expressed in mouse and human <t>synovium</t> and are functional in human fibroblast‐like synoviocytes <t>(FLS).</t> A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.
    Human Oa Fls Human Synovium, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human oa fls human synovium/product/Worthington Biochemical
    Average 90 stars, based on 1 article reviews
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    Worthington Biochemical oa biopsies
    Insulin receptors (IRs) are expressed in mouse and human <t>synovium</t> and are functional in human fibroblast‐like synoviocytes <t>(FLS).</t> A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.
    Oa Biopsies, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oa biopsies/product/Worthington Biochemical
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    Worthington Biochemical oa mice model
    Insulin receptors (IRs) are expressed in mouse and human <t>synovium</t> and are functional in human fibroblast‐like synoviocytes <t>(FLS).</t> A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.
    Oa Mice Model, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Insulin receptors (IRs) are expressed in mouse and human synovium and are functional in human fibroblast‐like synoviocytes (FLS). A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.

    Journal: Arthritis & Rheumatology (Hoboken, N.j.)

    Article Title: Suppressive Effects of Insulin on Tumor Necrosis Factor–Dependent Early Osteoarthritic Changes Associated With Obesity and Type 2 Diabetes Mellitus

    doi: 10.1002/art.39561

    Figure Lengend Snippet: Insulin receptors (IRs) are expressed in mouse and human synovium and are functional in human fibroblast‐like synoviocytes (FLS). A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.

    Article Snippet: Isolation and culture of human OA FLS Human synovium was minced and incubated with 1 mg/ml of type II collagenase (Worthington Biochemical) in serum‐free, standard Dulbecco's modified Eagle's medium (DMEM; Gibco) containing 1 gm/liter glucose, for 2 hours at 37°C.

    Techniques: Functional Assay, Immunohistochemistry, Staining, Negative Control, Expressing, Isolation, Western Blot