cardiomyocyte isolation kit  (Worthington Biochemical)


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    Name:
    Neonatal Cardiomyocyte Isolation System
    Description:
    Kit for performing five separate tissue dissociations each containing up to twelve hearts Contains single use vials of purified collagenase and trypsin CMF HBSS Leibovitz L 15 media and Falcon cell strainers along with a detailed protocol The kit is use tested by Worthington to assure performance
    Catalog Number:
    lk003300
    Price:
    256
    Size:
    1 kt
    Cas Number:
    see components
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    Structured Review

    Worthington Biochemical cardiomyocyte isolation kit
    Percentage (mean + SD) of ( a ) GM1, ( b ) [ 3 H]OA, and ( c ) [ 14 C]DHA in sucrose-gradient fractions of neonatal <t>cardiomyocytes.</t> Cells were incubated at 4°C in the presence of 1% Triton X-100, homogenized, and placed on a 5–40% sucrose gradient. After ultracentrifugation, fractions were collected in 1-mL increments from the top to bottom of the centrifuge tubes and analyzed (Materials and Methods). Values represent a minimum of three separate experiments.
    Kit for performing five separate tissue dissociations each containing up to twelve hearts Contains single use vials of purified collagenase and trypsin CMF HBSS Leibovitz L 15 media and Falcon cell strainers along with a detailed protocol The kit is use tested by Worthington to assure performance
    https://www.bioz.com/result/cardiomyocyte isolation kit/product/Worthington Biochemical
    Average 97 stars, based on 2350 article reviews
    Price from $9.99 to $1999.99
    cardiomyocyte isolation kit - by Bioz Stars, 2021-01
    97/100 stars

    Images

    1) Product Images from "Oleic and Docosahexaenoic Acid Differentially Phase Separate from Lipid Raft Molecules: A Comparative NMR, DSC, AFM, and Detergent Extraction Study"

    Article Title: Oleic and Docosahexaenoic Acid Differentially Phase Separate from Lipid Raft Molecules: A Comparative NMR, DSC, AFM, and Detergent Extraction Study

    Journal: Biophysical Journal

    doi: 10.1529/biophysj.104.044552

    Percentage (mean + SD) of ( a ) GM1, ( b ) [ 3 H]OA, and ( c ) [ 14 C]DHA in sucrose-gradient fractions of neonatal cardiomyocytes. Cells were incubated at 4°C in the presence of 1% Triton X-100, homogenized, and placed on a 5–40% sucrose gradient. After ultracentrifugation, fractions were collected in 1-mL increments from the top to bottom of the centrifuge tubes and analyzed (Materials and Methods). Values represent a minimum of three separate experiments.
    Figure Legend Snippet: Percentage (mean + SD) of ( a ) GM1, ( b ) [ 3 H]OA, and ( c ) [ 14 C]DHA in sucrose-gradient fractions of neonatal cardiomyocytes. Cells were incubated at 4°C in the presence of 1% Triton X-100, homogenized, and placed on a 5–40% sucrose gradient. After ultracentrifugation, fractions were collected in 1-mL increments from the top to bottom of the centrifuge tubes and analyzed (Materials and Methods). Values represent a minimum of three separate experiments.

    Techniques Used: Incubation

    2) Product Images from "Suppressive Effects of Insulin on Tumor Necrosis Factor–Dependent Early Osteoarthritic Changes Associated With Obesity and Type 2 Diabetes Mellitus"

    Article Title: Suppressive Effects of Insulin on Tumor Necrosis Factor–Dependent Early Osteoarthritic Changes Associated With Obesity and Type 2 Diabetes Mellitus

    Journal: Arthritis & Rheumatology (Hoboken, N.j.)

    doi: 10.1002/art.39561

    Insulin receptors (IRs) are expressed in mouse and human synovium and are functional in human fibroblast‐like synoviocytes (FLS). A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.
    Figure Legend Snippet: Insulin receptors (IRs) are expressed in mouse and human synovium and are functional in human fibroblast‐like synoviocytes (FLS). A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.

    Techniques Used: Functional Assay, Immunohistochemistry, Staining, Negative Control, Expressing, Isolation, Western Blot

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    Patch Clamp:

    Article Title: Pharmacologic TWIK‐Related Acid‐Sensitive K+ Channel (TASK‐1) Potassium Channel Inhibitor A293 Facilitates Acute Cardioversion of Paroxysmal Atrial Fibrillation in a Porcine Large Animal Model
    Article Snippet: .. Cardiomyocyte Isolation and Patch Clamp Measurements After transport in chilled Ca2+ ‐free solution (100 mmol/L NaCl, 10 mmol/L KCl, 1.2 mmol/L KH2 PO4 , 5 mmol/L MgSO4 , 50 mmol/L taurine, 5 mmol/L 3‐(N‐morpholino)propanesulfonic acid, 30 mmol/L 2,3‐butanedione monoxime and 20 mmol/L glucose, pH 7.0 with NaOH), right atrial human or porcine tissue samples were dissected into small chunks and rinsed 3 times for 3 minutes with Ca2+‐ free Tyrode's solution. .. Subsequently, tissue pieces were subjected to digestion with collagenase type I, 288 U/mL (Worthington Biochemical Corporation, Lakewood, NJ) and protease type XXIV, 5 mg/mL (Sigma‐Aldrich) in Ca2+ ‐free Tyrode's solution for 15 minutes.

    Article Title: Stilbene derivative as a photosensitive compound to control the excitability of neonatal rat cardiomyocytes
    Article Snippet: .. Preparation of neonatal rat ventricular cardiomyocyte (NRVM) for patch clamp for optical mapping Hearts were isolated from neonatal 1–2-day-old Wistar rats using a two-day protocol of Neonatal Cardiomyocyte Isolation System of Worthington Biochemical Corporation [ ] with some modifications. ..

    Isolation:

    Article Title: Construction of Cardiac Tissue Rings Using a Magnetic Tissue Fabrication Technique
    Article Snippet: .. Primary Culture of Neonatal Rat Cardiomyocytes Primary neonatal rat cardiomyocytes were isolated using a neonatal cardiomyocyte isolation kit (Worthington Biochemical, Lakewood, NJ, USA) according to the published procedure [ ]. .. Briefly, ventricles from 2–4 day-old Sprague-Dawley rats (Japan SLC, Inc., Hamamatsu, Japan) were incubated for 16–20 h at 4 °C in Hank’s balanced salt solution containing trypsin (50–100 μg/mL), followed by digestion with collagenase (75 U/mL) for 40 min at 37 °C.

    Article Title: Mitoregulin: A lncRNA-Encoded Microprotein that Supports Mitochondrial Supercomplexes and Respiratory Efficiency
    Article Snippet: .. Neonatal rat cardiomyocytes (NRCMs) were isolated using the Worthington Neonatal Cardiomyocyte Isolation System (Worthington Biochemical Corporation, Cat. No ). ..

    Article Title: ADAP1 limits neonatal cardiomyocyte hypertrophy by reducing integrin cell surface expression
    Article Snippet: .. RNVC were extracted from the hearts of Sprague Dawley rat pups (strain code 001; Charles River) 1–3 days after birth using the Neonatal Cardiomyocyte Isolation System (Worthington Biochemical Corporation). .. The isolated ventricles were incubated in calcium/magnesium-free Hank’s Balanced Salt Solution containing trypsin (50 µg/mL) in vented-cap tubes with slow agitation at 4 °C for 18 h. The trypsin was then inhibited by adding Soybean Trypsin Inhibitor (200 µg/mL), and the ventricles were further digested with collagenase (100 units/mL) at 37 °C for 30 min.

    Article Title: Pharmacologic TWIK‐Related Acid‐Sensitive K+ Channel (TASK‐1) Potassium Channel Inhibitor A293 Facilitates Acute Cardioversion of Paroxysmal Atrial Fibrillation in a Porcine Large Animal Model
    Article Snippet: .. Cardiomyocyte Isolation and Patch Clamp Measurements After transport in chilled Ca2+ ‐free solution (100 mmol/L NaCl, 10 mmol/L KCl, 1.2 mmol/L KH2 PO4 , 5 mmol/L MgSO4 , 50 mmol/L taurine, 5 mmol/L 3‐(N‐morpholino)propanesulfonic acid, 30 mmol/L 2,3‐butanedione monoxime and 20 mmol/L glucose, pH 7.0 with NaOH), right atrial human or porcine tissue samples were dissected into small chunks and rinsed 3 times for 3 minutes with Ca2+‐ free Tyrode's solution. .. Subsequently, tissue pieces were subjected to digestion with collagenase type I, 288 U/mL (Worthington Biochemical Corporation, Lakewood, NJ) and protease type XXIV, 5 mg/mL (Sigma‐Aldrich) in Ca2+ ‐free Tyrode's solution for 15 minutes.

    Article Title: Docosahexaenoic acid inhibits protein kinase C translocation/activation and cardiac hypertrophy in rat cardiomyocytes
    Article Snippet: .. Isolation of cardiomyocytes Neonatal cardiomyocytes were obtained using an isolation system from Worthington Biochemical Corporation. ..

    Article Title: Muscle ring finger protein-1 inhibits PKC? activation and prevents cardiomyocyte hypertrophy
    Article Snippet: .. NRVM were isolated using the neonatal cardiomyocyte isolation kit (Worthington) and were plated on laminin. ..

    Article Title: Pivotal role of cardiac lineage protein-1 (CLP-1) in transcriptional elongation factor P-TEFb complex formation in cardiac hypertrophy
    Article Snippet: .. Cardiac cells of 2–4-day-old WKY rats were isolated using a neonatal cardiomyocyte isolation system (Worthington Biochemical Corp, Lakewood, New Jersey, USA). .. After pre-plating to reduce contaminating non-muscle cells, cardiomyocytes were plated on Bioflex collagen 6-well plates (Flexcell, McKeesport, Pennsylvania, USA) and cultured in DMEM/F-12 (1:1) media supplemented with 10% fetal bovine serum for approximately 1.5 days prior to switching to serum-free medium.

    Article Title: Stilbene derivative as a photosensitive compound to control the excitability of neonatal rat cardiomyocytes
    Article Snippet: .. Preparation of neonatal rat ventricular cardiomyocyte (NRVM) for patch clamp for optical mapping Hearts were isolated from neonatal 1–2-day-old Wistar rats using a two-day protocol of Neonatal Cardiomyocyte Isolation System of Worthington Biochemical Corporation [ ] with some modifications. ..

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    Worthington Biochemical human oa fls human synovium
    Insulin receptors (IRs) are expressed in mouse and human <t>synovium</t> and are functional in human fibroblast‐like synoviocytes <t>(FLS).</t> A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.
    Human Oa Fls Human Synovium, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human oa fls human synovium/product/Worthington Biochemical
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Worthington Biochemical oa biopsies
    Insulin receptors (IRs) are expressed in mouse and human <t>synovium</t> and are functional in human fibroblast‐like synoviocytes <t>(FLS).</t> A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.
    Oa Biopsies, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oa biopsies/product/Worthington Biochemical
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    Worthington Biochemical oa mice model
    Insulin receptors (IRs) are expressed in mouse and human <t>synovium</t> and are functional in human fibroblast‐like synoviocytes <t>(FLS).</t> A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.
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    Image Search Results


    Insulin receptors (IRs) are expressed in mouse and human synovium and are functional in human fibroblast‐like synoviocytes (FLS). A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.

    Journal: Arthritis & Rheumatology (Hoboken, N.j.)

    Article Title: Suppressive Effects of Insulin on Tumor Necrosis Factor–Dependent Early Osteoarthritic Changes Associated With Obesity and Type 2 Diabetes Mellitus

    doi: 10.1002/art.39561

    Figure Lengend Snippet: Insulin receptors (IRs) are expressed in mouse and human synovium and are functional in human fibroblast‐like synoviocytes (FLS). A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.

    Article Snippet: Isolation and culture of human OA FLS Human synovium was minced and incubated with 1 mg/ml of type II collagenase (Worthington Biochemical) in serum‐free, standard Dulbecco's modified Eagle's medium (DMEM; Gibco) containing 1 gm/liter glucose, for 2 hours at 37°C.

    Techniques: Functional Assay, Immunohistochemistry, Staining, Negative Control, Expressing, Isolation, Western Blot