cardiomyocyte isolation kit  (Worthington Biochemical)


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    Name:
    Neonatal Cardiomyocyte Isolation System
    Description:
    Kit for performing five separate tissue dissociations each containing up to twelve hearts Contains single use vials of purified collagenase and trypsin CMF HBSS Leibovitz L 15 media and Falcon cell strainers along with a detailed protocol The kit is use tested by Worthington to assure performance
    Catalog Number:
    lk003300
    Price:
    256
    Size:
    1 kt
    Cas Number:
    see components
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    Structured Review

    Worthington Biochemical cardiomyocyte isolation kit
    Percentage (mean + SD) of ( a ) GM1, ( b ) [ 3 H]OA, and ( c ) [ 14 C]DHA in sucrose-gradient fractions of neonatal <t>cardiomyocytes.</t> Cells were incubated at 4°C in the presence of 1% Triton X-100, homogenized, and placed on a 5–40% sucrose gradient. After ultracentrifugation, fractions were collected in 1-mL increments from the top to bottom of the centrifuge tubes and analyzed (Materials and Methods). Values represent a minimum of three separate experiments.
    Kit for performing five separate tissue dissociations each containing up to twelve hearts Contains single use vials of purified collagenase and trypsin CMF HBSS Leibovitz L 15 media and Falcon cell strainers along with a detailed protocol The kit is use tested by Worthington to assure performance
    https://www.bioz.com/result/cardiomyocyte isolation kit/product/Worthington Biochemical
    Average 99 stars, based on 2351 article reviews
    Price from $9.99 to $1999.99
    cardiomyocyte isolation kit - by Bioz Stars, 2020-10
    99/100 stars

    Images

    1) Product Images from "Oleic and Docosahexaenoic Acid Differentially Phase Separate from Lipid Raft Molecules: A Comparative NMR, DSC, AFM, and Detergent Extraction Study"

    Article Title: Oleic and Docosahexaenoic Acid Differentially Phase Separate from Lipid Raft Molecules: A Comparative NMR, DSC, AFM, and Detergent Extraction Study

    Journal: Biophysical Journal

    doi: 10.1529/biophysj.104.044552

    Percentage (mean + SD) of ( a ) GM1, ( b ) [ 3 H]OA, and ( c ) [ 14 C]DHA in sucrose-gradient fractions of neonatal cardiomyocytes. Cells were incubated at 4°C in the presence of 1% Triton X-100, homogenized, and placed on a 5–40% sucrose gradient. After ultracentrifugation, fractions were collected in 1-mL increments from the top to bottom of the centrifuge tubes and analyzed (Materials and Methods). Values represent a minimum of three separate experiments.
    Figure Legend Snippet: Percentage (mean + SD) of ( a ) GM1, ( b ) [ 3 H]OA, and ( c ) [ 14 C]DHA in sucrose-gradient fractions of neonatal cardiomyocytes. Cells were incubated at 4°C in the presence of 1% Triton X-100, homogenized, and placed on a 5–40% sucrose gradient. After ultracentrifugation, fractions were collected in 1-mL increments from the top to bottom of the centrifuge tubes and analyzed (Materials and Methods). Values represent a minimum of three separate experiments.

    Techniques Used: Incubation

    2) Product Images from "Suppressive Effects of Insulin on Tumor Necrosis Factor–Dependent Early Osteoarthritic Changes Associated With Obesity and Type 2 Diabetes Mellitus"

    Article Title: Suppressive Effects of Insulin on Tumor Necrosis Factor–Dependent Early Osteoarthritic Changes Associated With Obesity and Type 2 Diabetes Mellitus

    Journal: Arthritis & Rheumatology (Hoboken, N.j.)

    doi: 10.1002/art.39561

    Insulin receptors (IRs) are expressed in mouse and human synovium and are functional in human fibroblast‐like synoviocytes (FLS). A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.
    Figure Legend Snippet: Insulin receptors (IRs) are expressed in mouse and human synovium and are functional in human fibroblast‐like synoviocytes (FLS). A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.

    Techniques Used: Functional Assay, Immunohistochemistry, Staining, Negative Control, Expressing, Isolation, Western Blot

    Related Articles

    Isolation:

    Article Title: Construction of Cardiac Tissue Rings Using a Magnetic Tissue Fabrication Technique
    Article Snippet: .. Primary Culture of Neonatal Rat Cardiomyocytes Primary neonatal rat cardiomyocytes were isolated using a neonatal cardiomyocyte isolation kit (Worthington Biochemical, Lakewood, NJ, USA) according to the published procedure [ ]. .. Briefly, ventricles from 2–4 day-old Sprague-Dawley rats (Japan SLC, Inc., Hamamatsu, Japan) were incubated for 16–20 h at 4 °C in Hank’s balanced salt solution containing trypsin (50–100 μg/mL), followed by digestion with collagenase (75 U/mL) for 40 min at 37 °C.

    Article Title: Mitoregulin: A lncRNA-Encoded Microprotein that Supports Mitochondrial Supercomplexes and Respiratory Efficiency
    Article Snippet: .. Neonatal rat cardiomyocytes (NRCMs) were isolated using the Worthington Neonatal Cardiomyocyte Isolation System (Worthington Biochemical Corporation, Cat. No ). ..

    Article Title: Single Cell Transcriptomics Reconstructs Fate Conversion from Fibroblast to Cardiomyocyte
    Article Snippet: .. Neonatal CMs were isolated using the neonatal cardiomyocytes isolation system (Worthington Biochemical Corporation) except that all enzymes were used at a ¼ of the recommended concentration to increase cell viability. .. After a 1.5 hour pre-plating on uncoated surface to remove attached nonmyocytes, the unattached CMs were collected in TRIzol ( > 80% viability by Trypan blue staining).

    Article Title: ADAP1 limits neonatal cardiomyocyte hypertrophy by reducing integrin cell surface expression
    Article Snippet: .. RNVC were extracted from the hearts of Sprague Dawley rat pups (strain code 001; Charles River) 1–3 days after birth using the Neonatal Cardiomyocyte Isolation System (Worthington Biochemical Corporation). .. The isolated ventricles were incubated in calcium/magnesium-free Hank’s Balanced Salt Solution containing trypsin (50 µg/mL) in vented-cap tubes with slow agitation at 4 °C for 18 h. The trypsin was then inhibited by adding Soybean Trypsin Inhibitor (200 µg/mL), and the ventricles were further digested with collagenase (100 units/mL) at 37 °C for 30 min.

    Article Title: Pro-survival function of MEF2 in cardiomyocytes is enhanced by β-blockers
    Article Snippet: .. Cell culture Primary neonatal rat cardiomyocytes were prepared from 1- to 3-day old Sprague Dawley rats using the Neonatal Cardiomyocyte Isolation System (Worthington Biochemical Corp, Lakewood, NJ, USA). .. Briefly, whole hearts were dissociated with trypsin (Promega, Madison, WI, USA) and collagenase (Worthington Biochemical Corp).

    Article Title: NO triggers RGS4 degradation to coordinate angiogenesis and cardiomyocyte growth
    Article Snippet: .. Neonatal rat cardiomyocytes were isolated from 2-day-old Sprague-Dawley rats using the Neonatal Cardiomyocyte Isolation System (Worthington Biochemical Corp.; ref. ). .. Isolated cardiomyocytes were plated on fibronectin-coated plates and cultured for 24 hours in DMEM supplemented with 10% FBS.

    Article Title: Docosahexaenoic acid inhibits protein kinase C translocation/activation and cardiac hypertrophy in rat cardiomyocytes
    Article Snippet: .. Isolation of cardiomyocytes Neonatal cardiomyocytes were obtained using an isolation system from Worthington Biochemical Corporation. ..

    Article Title: Muscle ring finger protein-1 inhibits PKC? activation and prevents cardiomyocyte hypertrophy
    Article Snippet: .. NRVM were isolated using the neonatal cardiomyocyte isolation kit (Worthington) and were plated on laminin. ..

    Cell Culture:

    Article Title: Pro-survival function of MEF2 in cardiomyocytes is enhanced by β-blockers
    Article Snippet: .. Cell culture Primary neonatal rat cardiomyocytes were prepared from 1- to 3-day old Sprague Dawley rats using the Neonatal Cardiomyocyte Isolation System (Worthington Biochemical Corp, Lakewood, NJ, USA). .. Briefly, whole hearts were dissociated with trypsin (Promega, Madison, WI, USA) and collagenase (Worthington Biochemical Corp).

    Concentration Assay:

    Article Title: Single Cell Transcriptomics Reconstructs Fate Conversion from Fibroblast to Cardiomyocyte
    Article Snippet: .. Neonatal CMs were isolated using the neonatal cardiomyocytes isolation system (Worthington Biochemical Corporation) except that all enzymes were used at a ¼ of the recommended concentration to increase cell viability. .. After a 1.5 hour pre-plating on uncoated surface to remove attached nonmyocytes, the unattached CMs were collected in TRIzol ( > 80% viability by Trypan blue staining).

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    Worthington Biochemical human oa fls human synovium
    Insulin receptors (IRs) are expressed in mouse and human <t>synovium</t> and are functional in human fibroblast‐like synoviocytes <t>(FLS).</t> A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.
    Human Oa Fls Human Synovium, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human oa fls human synovium/product/Worthington Biochemical
    Average 90 stars, based on 1 article reviews
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    Worthington Biochemical oa biopsies
    Insulin receptors (IRs) are expressed in mouse and human <t>synovium</t> and are functional in human fibroblast‐like synoviocytes <t>(FLS).</t> A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.
    Oa Biopsies, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oa biopsies/product/Worthington Biochemical
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    Worthington Biochemical oa mice model
    Insulin receptors (IRs) are expressed in mouse and human <t>synovium</t> and are functional in human fibroblast‐like synoviocytes <t>(FLS).</t> A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.
    Oa Mice Model, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Insulin receptors (IRs) are expressed in mouse and human synovium and are functional in human fibroblast‐like synoviocytes (FLS). A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.

    Journal: Arthritis & Rheumatology (Hoboken, N.j.)

    Article Title: Suppressive Effects of Insulin on Tumor Necrosis Factor–Dependent Early Osteoarthritic Changes Associated With Obesity and Type 2 Diabetes Mellitus

    doi: 10.1002/art.39561

    Figure Lengend Snippet: Insulin receptors (IRs) are expressed in mouse and human synovium and are functional in human fibroblast‐like synoviocytes (FLS). A and B, Immunohistochemical staining demonstrates abundance of IRs in a representative knee joint section from a C57BL/6 mouse (A) , in contrast to a representative knee joint section used as negative control (no primary antibody) (B) . Boxed areas in left panels are displayed at higher magnification in right panels. Original magnification × 50 in left panels; × 200 in right panels. C, Immunohistochemical staining demonstrates abundant expression of IRs in human synovium obtained from a patient with osteoarthritis (OA) undergoing total knee arthroplasty. Original magnification × 200. D, Isolated human OA FLS were treated without or with insulin at various concentrations for 5 minutes. IRβ autophosphorylation and phosphorylation of Akt (Ser 473 ) were assessed by Western blotting in immunoprecipitates and lysates, respectively. F = femur; T = tibia; M = meniscus; SM = synovial membrane.

    Article Snippet: Isolation and culture of human OA FLS Human synovium was minced and incubated with 1 mg/ml of type II collagenase (Worthington Biochemical) in serum‐free, standard Dulbecco's modified Eagle's medium (DMEM; Gibco) containing 1 gm/liter glucose, for 2 hours at 37°C.

    Techniques: Functional Assay, Immunohistochemistry, Staining, Negative Control, Expressing, Isolation, Western Blot