oa fls  (Worthington Biochemical)


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    Worthington Biochemical oa fls
    Oa Fls, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    oa mouse model  (Worthington Biochemical)


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    Worthington Biochemical oa mouse model
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    oa fls  (Worthington Biochemical)


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    Worthington Biochemical oa fls
    a Bone destruction as assessed by microCT analysis and toluidine blue stainings (scale bar: 50 µm) of paraffin sections from hind paws from 12 weeks old wt, Lasp1 −/− , hTNFtg and hTNFtg /Lasp1 −/− female mice ( n ≥ 8 in each group). Osteoclasts were visualised by TRAP stainings ( n ≥ 8 in each group, scale bar: 50 µm). b Body weight and clinical joint scores of wt, Lasp1 −/− , hTNFtg and hTNFtg /Lasp1 −/− mice from week 6 to 14 of age ( n ≥ 5 in each group). c Histomorphometric analysis of synovial pannus formation, length of synovial tissue attachment to joint cartilage, total cartilage area and destained cartilage in tarsal joints of hTNFtg and hTNFtg /Lasp1 −/− female mice ( n ≥ 8 each group). Data presented as mean ± SEM, * P < 0.05, ** P < 0.001 (two-tailed Mann–Whitney U test). d Quantification of TRAP + osteoclasts ( n ≥ 8 in each group). Data presented as mean ± SEM, * P = 0.01 (two-tailed Mann–Whitney U test). e Evaluation of RANKL/OPG ratio in <t>FLS</t> from arthritic and control mice ( n ≥ 5) by ELISA. Data presented as mean ± SEM, * P = 0.01 (two-tailed Mann–Whitney U test). f In vitro osteoclast differentiation assays and the tartrate resistant acid phosphatase (TRAP) stainings (representative images and numerical assessment of n = 3 independent experiments, scale bar: 100 µm). Data presented as mean ± SEM, * P = 0.01 (two-tailed Mann–Whitney U test). g MicroCT quantifications of bone volume/tissue volume (BV/TV) ratios in hTNFtg and hTNFtg /Lasp1 −/− mice ( n = 10 each group). Data presented as mean ± SEM, * P < 0.05, ** P < 0.001 (two-tailed Mann–Whitney U test). h Human TNF levels in the sera of hTNFtg and hTNFtg /Lasp1 −/− female mice ( n = 6 each group). Data presented as mean ± SEM (two-tailed Mann–Whitney U test). i Immunoblotting for Lasp1 in osteoblasts from wt and hTNFtg mice ( n = 3 each group). j Immunofluorescent stainings demonstrating the expression of Osterix in tissue from hTNFtg and hTNFtg /Lasp1 −/− mice. Antibody staining is in green and the nuclei with DAPI in blue (representative images of n = 3 independent experiments, scale bar: 50 µm). k Analysis of macrophages population <t>in</t> <t>collagenase</t> digested hind paws as determined by flow cytometry (representative images of n = 4 independent experiments). Source data are provided as a Source Data file.
    Oa Fls, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Lasp1 regulates adherens junction dynamics and fibroblast transformation in destructive arthritis"

    Article Title: Lasp1 regulates adherens junction dynamics and fibroblast transformation in destructive arthritis

    Journal: Nature Communications

    doi: 10.1038/s41467-021-23706-8

    a Bone destruction as assessed by microCT analysis and toluidine blue stainings (scale bar: 50 µm) of paraffin sections from hind paws from 12 weeks old wt, Lasp1 −/− , hTNFtg and hTNFtg /Lasp1 −/− female mice ( n ≥ 8 in each group). Osteoclasts were visualised by TRAP stainings ( n ≥ 8 in each group, scale bar: 50 µm). b Body weight and clinical joint scores of wt, Lasp1 −/− , hTNFtg and hTNFtg /Lasp1 −/− mice from week 6 to 14 of age ( n ≥ 5 in each group). c Histomorphometric analysis of synovial pannus formation, length of synovial tissue attachment to joint cartilage, total cartilage area and destained cartilage in tarsal joints of hTNFtg and hTNFtg /Lasp1 −/− female mice ( n ≥ 8 each group). Data presented as mean ± SEM, * P < 0.05, ** P < 0.001 (two-tailed Mann–Whitney U test). d Quantification of TRAP + osteoclasts ( n ≥ 8 in each group). Data presented as mean ± SEM, * P = 0.01 (two-tailed Mann–Whitney U test). e Evaluation of RANKL/OPG ratio in FLS from arthritic and control mice ( n ≥ 5) by ELISA. Data presented as mean ± SEM, * P = 0.01 (two-tailed Mann–Whitney U test). f In vitro osteoclast differentiation assays and the tartrate resistant acid phosphatase (TRAP) stainings (representative images and numerical assessment of n = 3 independent experiments, scale bar: 100 µm). Data presented as mean ± SEM, * P = 0.01 (two-tailed Mann–Whitney U test). g MicroCT quantifications of bone volume/tissue volume (BV/TV) ratios in hTNFtg and hTNFtg /Lasp1 −/− mice ( n = 10 each group). Data presented as mean ± SEM, * P < 0.05, ** P < 0.001 (two-tailed Mann–Whitney U test). h Human TNF levels in the sera of hTNFtg and hTNFtg /Lasp1 −/− female mice ( n = 6 each group). Data presented as mean ± SEM (two-tailed Mann–Whitney U test). i Immunoblotting for Lasp1 in osteoblasts from wt and hTNFtg mice ( n = 3 each group). j Immunofluorescent stainings demonstrating the expression of Osterix in tissue from hTNFtg and hTNFtg /Lasp1 −/− mice. Antibody staining is in green and the nuclei with DAPI in blue (representative images of n = 3 independent experiments, scale bar: 50 µm). k Analysis of macrophages population in collagenase digested hind paws as determined by flow cytometry (representative images of n = 4 independent experiments). Source data are provided as a Source Data file.
    Figure Legend Snippet: a Bone destruction as assessed by microCT analysis and toluidine blue stainings (scale bar: 50 µm) of paraffin sections from hind paws from 12 weeks old wt, Lasp1 −/− , hTNFtg and hTNFtg /Lasp1 −/− female mice ( n ≥ 8 in each group). Osteoclasts were visualised by TRAP stainings ( n ≥ 8 in each group, scale bar: 50 µm). b Body weight and clinical joint scores of wt, Lasp1 −/− , hTNFtg and hTNFtg /Lasp1 −/− mice from week 6 to 14 of age ( n ≥ 5 in each group). c Histomorphometric analysis of synovial pannus formation, length of synovial tissue attachment to joint cartilage, total cartilage area and destained cartilage in tarsal joints of hTNFtg and hTNFtg /Lasp1 −/− female mice ( n ≥ 8 each group). Data presented as mean ± SEM, * P < 0.05, ** P < 0.001 (two-tailed Mann–Whitney U test). d Quantification of TRAP + osteoclasts ( n ≥ 8 in each group). Data presented as mean ± SEM, * P = 0.01 (two-tailed Mann–Whitney U test). e Evaluation of RANKL/OPG ratio in FLS from arthritic and control mice ( n ≥ 5) by ELISA. Data presented as mean ± SEM, * P = 0.01 (two-tailed Mann–Whitney U test). f In vitro osteoclast differentiation assays and the tartrate resistant acid phosphatase (TRAP) stainings (representative images and numerical assessment of n = 3 independent experiments, scale bar: 100 µm). Data presented as mean ± SEM, * P = 0.01 (two-tailed Mann–Whitney U test). g MicroCT quantifications of bone volume/tissue volume (BV/TV) ratios in hTNFtg and hTNFtg /Lasp1 −/− mice ( n = 10 each group). Data presented as mean ± SEM, * P < 0.05, ** P < 0.001 (two-tailed Mann–Whitney U test). h Human TNF levels in the sera of hTNFtg and hTNFtg /Lasp1 −/− female mice ( n = 6 each group). Data presented as mean ± SEM (two-tailed Mann–Whitney U test). i Immunoblotting for Lasp1 in osteoblasts from wt and hTNFtg mice ( n = 3 each group). j Immunofluorescent stainings demonstrating the expression of Osterix in tissue from hTNFtg and hTNFtg /Lasp1 −/− mice. Antibody staining is in green and the nuclei with DAPI in blue (representative images of n = 3 independent experiments, scale bar: 50 µm). k Analysis of macrophages population in collagenase digested hind paws as determined by flow cytometry (representative images of n = 4 independent experiments). Source data are provided as a Source Data file.

    Techniques Used: Two Tailed Test, MANN-WHITNEY, Enzyme-linked Immunosorbent Assay, In Vitro, Western Blot, Expressing, Staining, Flow Cytometry

    oa biopsies  (Worthington Biochemical)


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    oa mice model  (Worthington Biochemical)


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    oa mice model  (Worthington Biochemical)


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    Worthington Biochemical oa mice model
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    oa joints  (Worthington Biochemical)


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    Worthington Biochemical oa joints
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    oa joints  (Worthington Biochemical)


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    oa joints  (Worthington Biochemical)


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    oa chondrocytes  (Worthington Biochemical)


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