Iberiotoxin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Differential Expression of Large-Conductance Ca2+-Activated K Channels in the Carotid Body between DBA/2J and A/J Strains of Mice"
Article Title: Differential Expression of Large-Conductance Ca2+-Activated K Channels in the Carotid Body between DBA/2J and A/J Strains of Mice
Journal: Frontiers in Cellular Neuroscience
Figure Legend Snippet: For patch clamp experiments, undissociated CBs were used . The setup (A) and the image of the CB (B) are shown. Examples of K current records and mean I – V relationships in the DBA/2J (C) and the A/J mouse (D) are presented. Whole current traces were evoked by voltage steps from −80 to +60 mV with 10 mV increments for 100 ms. To construct a current–voltage curve, the mean current measured between 93 and 97 ms from the start of each voltage step. Mean currents were plotted versus the test pulse. Bars represent SEM. No statistical differences were seen between the strains. (E) The effect of iberiotoxin (a BK channel blocker; 200 nM) on outward current in GCs. The outward current was evoked by a test pulse from −80 to +20 mV for 100 ms. Iberiotoxin significantly and reversibly decreased outward current in GCs of the DBA/2J mice. However, K current in the A/J mice was not significantly affected by iberiotoxin. *, Significantly different from control and recovery. The amplitudes of K current (control and recovery) were significantly larger in GCs of the DBA/2J mice. Cont, control; Ibx, iberiotoxin; Rec, recovery.
Techniques Used: Patch Clamp, Construct, Mouse Assay
2) Product Images from "Epoxyeicosatrienoic and dihydroxyeicosatrienoic acids dilate human coronary arterioles via BKCa channels: implications for soluble epoxide hydrolase inhibition"
Article Title: Epoxyeicosatrienoic and dihydroxyeicosatrienoic acids dilate human coronary arterioles via BKCa channels: implications for soluble epoxide hydrolase inhibition
Figure Legend Snippet: Effect of large-conductance Ca 2+ -activated K + (BK Ca ) channel blockade on EET- and dihydroxyeicosatrienoic acids (DHET)-induced dilation of HCAs. A–C : dilation to 8,9-, 11,12-, and 14,15-EET is inhibited by iberiotoxin (100 nmol/l, n = 6, 7, and
3) Product Images from "Serum-induced changes in the physiology of mammalian retinal glial cells: role of lysophosphatidic acid"
Article Title: Serum-induced changes in the physiology of mammalian retinal glial cells: role of lysophosphatidic acid
Journal: The Journal of Physiology
Figure Legend Snippet: Effects of charybdotoxin, iberiotoxin and margatoxin on Müller cell currents induced by serum A , top panel, I - V relationship under control conditions and in the presence of either 10% bovine serum, 100 nM charybdotoxin (ChTX) or serum plus ChTX. Bottom panel, plot of the difference between the I - V curves of the serum-induced currents in the absence and presence of ChTX. B , similar to A , with 50 nM iberiotoxin (IbTX) in place of ChTX. C , similar to A and B , with 1 nM margatoxin (MTX) in place of ChTX or IbTX. Freshly dissociated bovine Müller cells were monitored with perforated-patch recordings. The sensitivity of the serum-induced outwardly rectifying current to charybdotoxin and margatoxin, but not iberiotoxin, is consistent with the activation of K V 1.3 channels.
Techniques Used: Activation Assay
4) Product Images from "Study of membrane potential in T lymphocytes subpopulations using flow cytometry"
Article Title: Study of membrane potential in T lymphocytes subpopulations using flow cytometry
Journal: BMC Immunology
Figure Legend Snippet: FACS measurement of ψ distinguishes CHO-Kv1.3 from CHO cells . Histograms of oxonol fluorescence in CHO (A, D), CHO-Kv1.3 (B, E) and a combination of CHO and CHO-Kv1.3 (C, F) untreated (gray lines) or treated (black lines) with MgTX (A, B, C) or IbTX (D, E, F). The ψ values in mV are depicted. Data are representative of four experiments.
Techniques Used: FACS, Fluorescence
Figure Legend Snippet: Validation of ψ quantification by FACS . Representative trace of a CHO-Kv1.3 cell treated with different concentrations of [K + ] e recorded by patch-clamp (A) or by FACS (B). (C) Dependence of absolute ψ of CHO-Kv1.3 cells on the [K + ] e determined by FACS (●) and patch-clamp (Δ) techniques. Data of ψ against increasing [K + ] e (5–145 mM) were plotted and fitted with a Boltzman equation. Data represent mean ± SE of four experiments. (D) CHO-Kv1.3 cells were treated with different concentrations (1, 3 and 10 nM) of MgTx (black bars) or IbTx (grey bars) and their ψ was measured by FACS. Data represent mean ± SD of three experiments.
Techniques Used: FACS, Patch Clamp
5) Product Images from "BK channel overexpression on plasma membrane of fibroblasts from Hutchinson-Gilford progeria syndrome"
Article Title: BK channel overexpression on plasma membrane of fibroblasts from Hutchinson-Gilford progeria syndrome
Journal: Aging (Albany NY)
Figure Legend Snippet: Proliferation and adhesion rates without and with the BK Ca inhibitor IbTx. ( A ) Average number of hDF obtained from young healthy donors and patients affected by HGPS estimated at 48, 72 and 96 hours from seeding and normalized at 24 h (fold-change ± SEM). Healthy and HGPS hDF were allowed to proliferate untreated (Young n=54; HGPS n=36) and treated by 100 nM IbTx (Young n=53; HGPS n=36). Young vs. HGPS: **p
Figure Legend Snippet: Outward currents patch-clamp recorded in whole-cell configuration. ( A ) Representative examples of current traces recorded in hDF obtained from a young donor, an elderly, and a patient affected by HGPS. Current traces recorded after 100 nM IbTx application and a graphical representation of the pulse protocol (holding potential at 0 mV) are also shown. ( B ) Average ± SEM of current-voltage relationships (I–V) recorded in hDF obtained from healthy donors (Young, n=83; Elderly, n=16) and patients affected by HGPS (n=80). ( C ) Average ± SEM of current-voltage relationships (I–V) recorded in hDF obtained from young donors and patients affected by HGPS treated by 100 nM IbTx (n=6) and 10 mM TEA (n=4). Young vs. HGPS: *p
Techniques Used: Patch Clamp
6) Product Images from "Characterisation of K+ Channels in Human Fetoplacental Vascular Smooth Muscle Cells"
Article Title: Characterisation of K+ Channels in Human Fetoplacental Vascular Smooth Muscle Cells
Journal: PLoS ONE
Figure Legend Snippet: Characterisation of Ca 2+ -activated K + channel isoforms in CPASMCs. Representative example of the inhibition of outward currents by the BK Ca and IK Ca blocker charybdotoxin (ChTx; n = 4; N = 2; 100 nM; A ), but not the specific IK Ca inhibitor TRAM-34 (n = 3, N = 2; 10µM; B ). The specific BK Ca blocker iberiotoxin (IbTx; 100 nM; C ) inhibited outward currents at depolarised potentials. Mean current-voltage relationships measured at the end of the 500 ms voltage step ranging from -70 mV to +80 mV were obtained in the absence (•) and presence (○) of IbTx ( D ; *P
Techniques Used: Inhibition, Mass Spectrometry
7) Product Images from "Ability of naringenin, a bioflavonoid, to activate M-type potassium current in motor neuron-like cells and to increase BKCa-channel activity in HEK293T cells transfected with α-hSlo subunit"
Article Title: Ability of naringenin, a bioflavonoid, to activate M-type potassium current in motor neuron-like cells and to increase BKCa-channel activity in HEK293T cells transfected with α-hSlo subunit
Journal: BMC Neuroscience
Figure Legend Snippet: Effects of NGEN and other K + current blockers on the amplitude of I K(M) in NSC-34 cells. In these experiments, cells were bathed in Ca 2+ -free Tyrode's solution which contained 200 nM iberiotoxin, 200 nM apamin and 1 μM tetrodotoxin, and each cell was hyperpolarized from -20 to -50 mV with a duration of 2 sec. Current amplitude was measured at the end of voltage pulse. Each bar represents the mean ± SEM (n = 7-13). Flu: flupirtine; Iber: iberiotoxin; Aps: apamin; Lino: linopirdine. * Significantly different from control. ** Significantly different from NGEN (10 μM) alone group. Notably, further application of linopirdine reversed NGEN-stimulated I K(M) , while neither iberiotoxin nor apamin produced any effects on it.
Techniques Used: Produced
Figure Legend Snippet: Effects of NGEN on I K(M) recorded from motor neuron-like NSC-34 cells. In (A) , superimposed current traces obtained in the absence (left) and presence (right) of 10 μM NGEN. In these experiments, cells were bathed Ca 2+ -Tyrode’s solution which contained 200 nM iberiotoxin, 200 nM apamin and 1 μM tetrodotoxin. The I K(M) was elicited from -20 mV to different potentials which ranged from -50 to +10 mV with 10-mV increments. (B) Effect of NGEN on the averaged I-V relations of I K(M) in NSC-34 cells (mean ± SEM; n = 9-13 for each point). ■: control; □: 10 μM NGEN. Current amplitude was measured at end of each voltage pulse. (C) Voltage dependence of I K(M) conductance in the absence (■) and presence (□) of 10 μM NGEN (mean ± SEM; n = 8-12 for each point). Note that there is a leftward shift in the activation curve of I K(M) conductance during cell exposure to NGEN, although the slope factor remains unchanged.
Techniques Used: Activation Assay
Figure Legend Snippet: Effect of NGEN on whole-cell I K(Ca) in α -hSlo -expressing HEK293T cells. In these experiments, cells were bathed in normal Tyrode’s solution containing 1.8 mM CaCl 2 . (A) Current traces in response to membrane depolarization from 0 to +50 mV. The upper part indicates the voltage protocol used. a: control; b: 3 μM NGEN; c: 10 μM NGEN; and d: 30 μM NGEN. (B) Summary of the data showing effects of NGEN, NGEN plus verruculogen (Verr; 1 μM) and NGEN plus iberiotoxin (Iber; 200 nM) on I K(Ca) amplitude in these cells (mean ± SEM; n = 10-13 for each bar). * Significantly different from control. ** Significantly different from NGEN (30 μM) alone group.
Techniques Used: Expressing
Figure Legend Snippet: Stimulatory effect of NGEN on the activity of K M channels recorded from NSC-34 cells. In (A) , cells were bathed in Ca 2+ -free Tyrode’s solution which contained iberiotoxin (200 nM), apamin (200 nM) and tetrodotoxin (1 μM). Cell-attached configuration was made as the cell attached was held at 0 mV relative to the bath. Channel activity was obtained in the control (left) and after addition of NGEN (10 μM) to the bath. Portion of tracing in the upper part of (A) is amplified in the lower part. Channel openings give a downward deflection in current. (B) Summary of the data showing effects of NGEN and flupirtine (Flu) on K M -channel activity in NSC-34 cells (mean ± SEM; n = 9-12 for each bar). * Significantly different from control.
Techniques Used: Activity Assay, Amplification
8) Product Images from "Inhibition of vascular calcium-gated chloride currents by blockers of KCa1.1, but not by modulators of KCa2.1 or KCa2.3 channels"
Article Title: Inhibition of vascular calcium-gated chloride currents by blockers of KCa1.1, but not by modulators of KCa2.1 or KCa2.3 channels
Journal: British Journal of Pharmacology
Figure Legend Snippet: Modulation of spontaneous transient outward currents (STOCs) by anti-K Ca 1.1 antibody. (A–C) Representative recordings of STOCs at 0 mV under control conditions, after 5 min application of 10 nM iberiotoxin (B) or after 10 min intracellular dialysis
Figure Legend Snippet: Effects of penitrem A and iberiotoxin on I ClCa . (A) The effect of 10 µM penitrem A (Pen) on I ClCa . (A)i shows representative currents recorded in the absence and presence of penitrem A (5 min, 1 µM). (A)ii and (A)iii show the effect of
9) Product Images from "Activation of TRPV4 channels leads to a consistent tocolytic effect on human myometrial tissues"
Article Title: Activation of TRPV4 channels leads to a consistent tocolytic effect on human myometrial tissues
Journal: European Journal of Obstetrics & Gynecology and Reproductive Biology: X
Figure Legend Snippet: Tocolytic effects of 4αPDD and GSK1016790A were reversed by ruthenium red and IbTX. A: Tocolytic effect of 100 and 300 nM 4αPDD on the oxytocin-induced contractile activity. This effect was reversed by 5 μM Ruthenium Red. B: This recording demonstrates that the tocolytic effect of 100 nM GSK1016790A on the uterotonic-induced contractile activity (300 nM PGF 2α and 100 nM oxytocin) was reversed by 50 nM IbTX, a specific inhibitor of BKCa channels. C: This recording illustrates the difference of tocolytic mechanism between 100 nM GSK1016790A and 300 nM Nifedipine. The former results in the full inactivation of contractions, while the latter reduced the amplitude of the contractions, likely by blocking the L-type Ca 2+ channels.
Techniques Used: Activity Assay, Blocking Assay
Figure Legend Snippet: Graphic summary: Involvement of TRPV4 and BKCa activation results in a strong tocolytic effect on human myometrial strips. The TRPV4 agonist (GSK1016790A) may exert a tocolytic effect on human myometrial strips by activating the BKCa channels. This strong inhibitory effect is likely due to a localized Ca 2+ entry, which in turn activates BKCa channels. An increase in open state probability of BKCa channels results in membrane hyperpolarization and concomitant inactivation of Nifedipine-sensitive Ca 2+ channels, while either ruthenium red (a TRPV4 blocker), or iberiotoxin (IbTX) -a high-affinity BKCa blocker- reverses this tocolytic effect [ 17 ].
Techniques Used: Activation Assay