dnase ii  (Worthington Biochemical)


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  • 99
    Name:
    Deoxyribonuclease I
    Description:
    Chromatographically purified A lyophilized powder with glycine as a stabilizer
    Catalog Number:
    ls002004
    Price:
    33
    Size:
    5 mg
    Source:
    Bovine Pancreas
    Cas Number:
    9003.98.9
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    Structured Review

    Worthington Biochemical dnase ii
    Chromatographically purified A lyophilized powder with glycine as a stabilizer
    https://www.bioz.com/result/dnase ii/product/Worthington Biochemical
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    dnase ii - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    In Vitro:

    Article Title: Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis
    Article Snippet: .. Treatment with DNase I eliminated the presence of released DNA, but did not block killing of the larvae by mouse neutrophils and macrophages in vitro. .. This observation suggests that in contrast to human neutrophils and macrophages, mouse cells do not require ET formation in vitro to kill the worms.

    Positive Control:

    Article Title: STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells
    Article Snippet: .. Concentration ranges for DNase I were determined empirically for each lot and cell type by titrating DNase I for its ability to digest CR-C as the positive control but not regions previously found to be resistant to DNase I (e.g., +6.3 region). .. Following purification of the digested DNA, PCR was performed across the Pdcd1 locus using a set of 59 primer pairs ( ).

    Blocking Assay:

    Article Title: Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis
    Article Snippet: .. Treatment with DNase I eliminated the presence of released DNA, but did not block killing of the larvae by mouse neutrophils and macrophages in vitro. .. This observation suggests that in contrast to human neutrophils and macrophages, mouse cells do not require ET formation in vitro to kill the worms.

    Concentration Assay:

    Article Title: Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA
    Article Snippet: .. DNase I (Worthington) diluted in DNase I dilution buffer (2.5 mM MgCl2 , 0.5 mM CaCl2 , 10 mM Tris-HCl, pH 7.6, 0.1 mg/ml BSA) to a final concentration of 0.0625 μg/ml was incubated with reaction mixtures for 1 min. ..

    Article Title: STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells
    Article Snippet: .. Concentration ranges for DNase I were determined empirically for each lot and cell type by titrating DNase I for its ability to digest CR-C as the positive control but not regions previously found to be resistant to DNase I (e.g., +6.3 region). .. Following purification of the digested DNA, PCR was performed across the Pdcd1 locus using a set of 59 primer pairs ( ).

    Incubation:

    Article Title: Epigenetic Control of Cell Cycle-Dependent Histone Gene Expression Is a Principal Component of the Abbreviated Pluripotent Cell Cycle
    Article Snippet: .. Nuclei were then resuspended in RSB buffer supplemented with 1 mM CaCl2 and incubated with increasing concentrations of DNase I (DPRF; Worthington Biochemical Corporation, Lakewood, NJ) for 10 min at room temperature with gentle agitation. ..

    Article Title: Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA
    Article Snippet: .. DNase I (Worthington) diluted in DNase I dilution buffer (2.5 mM MgCl2 , 0.5 mM CaCl2 , 10 mM Tris-HCl, pH 7.6, 0.1 mg/ml BSA) to a final concentration of 0.0625 μg/ml was incubated with reaction mixtures for 1 min. ..

    other:

    Article Title: Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA
    Article Snippet: The region is dA+dT rich, and protection seen from positions +126 to +129 is subtle because DNase I does not cleave DNA efficiently in this region.

    Article Title: Structural and functional conservation at the boundaries of the chicken ?-globin domain
    Article Snippet: The DNase I HS, 3′HS, was detected by digestion of nuclei with increasing amounts of DNase I as above followed by digestion of extracted DNA with Kpn I and probing with i112 (1000 bp; Kpn I –Pst I at 23.3–24.3 map units).

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  • 99
    Worthington Biochemical dnase ii
    Dnase Ii, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase ii/product/Worthington Biochemical
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    dnase ii - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

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