dnase ii  (Worthington Biochemical)


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  • 99
    Name:
    Deoxyribonuclease I
    Description:
    Chromatographically purified A lyophilized powder with glycine as a stabilizer
    Catalog Number:
    LS002004
    Price:
    33
    Source:
    Bovine Pancreas
    Size:
    5 mg
    Cas Number:
    9003.98.9
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    Structured Review

    Worthington Biochemical dnase ii
    Chromatographically purified A lyophilized powder with glycine as a stabilizer
    https://www.bioz.com/result/dnase ii/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase ii - by Bioz Stars, 2021-04
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: NuMA Influences Higher Order Chromatin Organization in Human Mammary Epithelium
    Article Snippet: .. DNase I (Worthington Biochemical) was added to a final concentration of 130 μg/ml. .. To aid the removal of cut DNA, (NH4 )2 SO4 was added to a final concentration of 0.25 M and the samples were incubated for 5 min at room temperature.

    other:

    Article Title: DNase I aggravates islet β-cell apoptosis in type 2 diabetes
    Article Snippet: DNase I is ubiquitously expressed in mammalian tissues, particularly in the pancreas ( ).

    Article Title: Rapid and Unambiguous Detection of DNase I Hypersensitive Site in Rare Population of Cells
    Article Snippet: To increase the reproducibility and minimize experimental variations, we chose to digest the nuclei of CD4 T cells with various amounts of DNase I on ice for a relatively long period of time (1 hr).

    End Labeling:

    Article Title: Nucleosomes Are Translationally Positioned on the Active Allele and Rotationally Positioned on the Inactive Allele of the HPRT Promoter
    Article Snippet: The positions of the MNase cleavages within chromatin of the HPRT promoter region of permeabilized cells relative to a downstream Bcl I site in the first intron of the HPRT gene were mapped using a 400-bp hybridization probe located just upstream of the Bcl I site (Fig. ). .. To determine the positions of DNase I-hypersensitive sites relative to MNase cleavage sites, NP-40-permeabilized cells containing the active HPRT allele were treated with increasing concentrations of DNase I and the DNase I-hypersensitive sites in chromatin of the HPRT promoter relative to the same Bcl I site were also mapped by indirect end labeling using the same hybridization probe. .. Figure shows the Southern blot analysis of the DNase I and MNase cleavage patterns on the inactive and active HPRT promoters in permeabilized 8121 and 4.12 cells, respectively.

    Hybridization:

    Article Title: Nucleosomes Are Translationally Positioned on the Active Allele and Rotationally Positioned on the Inactive Allele of the HPRT Promoter
    Article Snippet: The positions of the MNase cleavages within chromatin of the HPRT promoter region of permeabilized cells relative to a downstream Bcl I site in the first intron of the HPRT gene were mapped using a 400-bp hybridization probe located just upstream of the Bcl I site (Fig. ). .. To determine the positions of DNase I-hypersensitive sites relative to MNase cleavage sites, NP-40-permeabilized cells containing the active HPRT allele were treated with increasing concentrations of DNase I and the DNase I-hypersensitive sites in chromatin of the HPRT promoter relative to the same Bcl I site were also mapped by indirect end labeling using the same hybridization probe. .. Figure shows the Southern blot analysis of the DNase I and MNase cleavage patterns on the inactive and active HPRT promoters in permeabilized 8121 and 4.12 cells, respectively.

    Expressing:

    Article Title: DNase I aggravates islet β-cell apoptosis in type 2 diabetes
    Article Snippet: In summary, the present study provided novel insight into the central role of DNase I in high glucose-induced pancreatic β-cell apoptosis. .. It was demonstrated that high glucose was able to increase the expression of DNase I, and that this may aggravate β-cell apoptosis. ..

    Cell Culture:

    Article Title: Evaluation of the Functional Involvement of Human Immunodeficiency Virus Type 1 Integrase in Nuclear Import of Viral cDNA during Acute Infection
    Article Snippet: Pseudotype viruses were prepared by cotransfection of 293T cells with the pNL43lucΔenv vector together with the MuLV envelope expression vector (pJD-1). .. After treatment with DNase I (40 μg/ml; Worthington), each virus (70 ng of p24) was inoculated into HeLa cells and cultured at 37°C for 6 h. The cells were washed with PBS and resuspended in fresh medium (DMEM plus 10% FBS). ..

    Staining:

    Article Title: NuMA Influences Higher Order Chromatin Organization in Human Mammary Epithelium
    Article Snippet: To verify that the DNase I treatment mentioned in the previous paragraph indeed affected chromatin components, we analyzed the staining pattern of chromatin markers acetyl-H4 and H4K20m ( ; ) after DNase I treatment. .. Staining for these proteins was almost totally eliminated in cells treated with DNase I, whereas the staining pattern for SC35, indicative of nonchromatin splicing speckles, seemed unaltered ( , G–J). .. The quantitative analysis of the distribution of NuMA in differentiated S1 cells that have not undergone DNase I treatment reveals that NuMA staining foci are abundant in the midnuclear region ( D; ).

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  • 99
    Worthington Biochemical dnase ii
    Dnase Ii, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase ii/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase ii - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

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