dnase ii (Worthington Biochemical)

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Name:

Deoxyribonuclease I

Description:

Chromatographically purified A lyophilized powder with glycine as a stabilizer

Catalog Number:

ls002004

Price:

Size:

5 mg

Source:

Bovine Pancreas

Cas Number:

9003.98.9

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Structured Review

Worthington Biochemical dnase ii
Chromatographically purified A lyophilized powder with glycine as a stabilizer
https://www.bioz.com/result/dnase ii/product/Worthington Biochemical
Average 99 stars, based on 11 article reviews
Price from $9.99 to $1999.99

dnase ii - by Bioz Stars, 2021-01

99/100 stars

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Images

In Vivo:

Article Title: Nucleosomes Are Translationally Positioned on the Active Allele and Rotationally Positioned on the Inactive Allele of the HPRT Promoter
Article Snippet: .. In vivo DNase I footprint analysis was performed by treating permeabilized 4.12 and 8121 cells with increasing concentrations of DNase I, purifying the DNase I-treated genomic DNA, and examining the DNase I cleavage pattern in the region of interest by LMPCR. ..

Concentration Assay:

Article Title: A Cellular Protein Binds Vaccinia Virus Late Promoters and Activates Transcription In Vitro
Article Snippet: .. DNase I (Worthington Biochemicals) was added to a concentration of 8 ng/ml, and incubation was continued for an additional 2 min, after which the cleavage reaction was terminated by addition of an equal volume of a stop solution (0.2 M NaCl, 30 mM EDTA, 1% sodium dodecyl sulfate, and 100 μg of glycogen/ml). .. DNA cleavage products were extracted with phenol-chloroform, precipitated with ethanol, and analyzed on a 6% polyacrylamide DNA sequencing gel.

Incubation:

other:

Article Title: Structural and functional conservation at the boundaries of the chicken ?-globin domain
Article Snippet: The DNase I HS, 3′HS, was detected by digestion of nuclei with increasing amounts of DNase I as above followed by digestion of extracted DNA with Kpn I and probing with i112 (1000 bp; Kpn I –Pst I at 23.3–24.3 map units).

Article Title: Structural and functional conservation at the boundaries of the chicken ?-globin domain
Article Snippet: [ ] [ ] Stalder J., Larsen,A., Engel,J.D., Dolan,M., Groudine,M. and Weintraub,H. (1980) Tissue-specific DNA cleavages in the globin chromatin domain introduced by DNase I.

Activity Assay:

Article Title: Dynamic BRG1 Recruitment during T Helper Differentiation and Activation Reveals Distal Regulatory Elements ▿Dynamic BRG1 Recruitment during T Helper Differentiation and Activation Reveals Distal Regulatory Elements ▿ §
Article Snippet: .. Newly identified distal BRG1 binding regions in the GATA3 locus were specific with respect to fate and hypersensitive to DNase I and possessed BRG1-dependent enhancer-like activity. .. BRG1 binding to these regions was dependent on the presence of STAT6.

Sequencing:

Article Title: Role of the Promoter in Maintaining Transcriptionally Active Chromatin Structure and DNA Methylation Patterns In Vivo
Article Snippet: .. It is possible that the steeper slope detected by this probe is indicative of an intrinsically higher accessibility of the 5′flanking region to DNase I (or preferential DNase I cleavage of the underlying DNA sequence). ..

Staining:

Article Title: Constitutive Nucleosome Depletion and Ordered Factor Assembly at the GRP78 Promoter Revealed by Single Molecule Footprinting
Article Snippet: .. These were then digested at 37 °C for 15 min using various concentrations of DNase I (Worthington, San Francisco, California, United States) to obtain a suitable range of digestion of genomic DNA as revealed by EtBr staining. .. Digested genomic DNA was purified, redigested by RsaI, resolved on a 1.5% agarose gel, and Southern blotted.