dnase ii  (Worthington Biochemical)


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  • 94
    Name:
    Deoxyribonuclease II
    Description:
    A dialyzed lyophilized powder
    Catalog Number:
    LS002425
    Price:
    66
    Source:
    Porcine Spleen
    Size:
    80 ku
    Cas Number:
    9025.64.3
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    Structured Review

    Worthington Biochemical dnase ii
    A dialyzed lyophilized powder
    https://www.bioz.com/result/dnase ii/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase ii - by Bioz Stars, 2021-07
    94/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Extracellular Tau Oligomers Induce Invasion of Endogenous Tau into the Somatodendritic Compartment and Axonal Transport Dysfunction
    Article Snippet: Briefly, brains were removed from the embryos and placed in Hank’s balanced salt solution (HBSS; Invitrogen) in a sterile environment, after which the cortical tissue was isolated following removal of the meninges. .. The trypsin protease activity was quenched with heat-inactivated fetal bovine serum (FBS; HyClone/GE Healthcare), and the cells were washed 3 times with warm HBSS and treated with 100 units DNAse II (Worthington) per brain. .. The cells were then mechanically dissociated with Pasteur pipettes, diluted in Neurobasal media containing serum-free neuron supplement B27, l-glutamine, glucose, penicillin/streptomycin (all from Invitrogen) and 5% FBS, and plated at roughly 6250 cells/cm2 .

    Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
    Article Snippet: The background was subtracted prior to the calculation of DNase I activity. .. The percentage of DNase I activity was calculated using Equation 1: DNase\u00a0I\u00a0activity (%) =\u00a0 (mean\u00a0velocity\u00a0of\u00a0a\u00a0compound/mean\u00a0velocity\u00a0of\u00a0DMSO)\u00a0\u00d7\u00a0100 (1) In similar assays, recombinant murine EndoG (produced in-house) was used at a concentration of 0.14 μM in 0.1 mM MgCl2 , 10 mM Tris-HCl, pH 7.4; and DNase II (Worthington, Lakewood, NJ) (3.32 nM) was tested in 100 mM sodium citrate buffer, pH 5.0. .. For evaluation of the quality of the assay, Z’ values were calculated using Equation 2: Z\u2019 =\u00a01\u00a0\u2212\u00a0(3SDC +\u00a03SDB )/(MC \u00a0\u2212\u00a0MB ) (2) where M = mean value; SD = standard deviation; C = control; and B = background.

    Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
    Article Snippet: It should be noted that the k cat / K m value of EndoG is not well defined due to the poor activity on the substrate. .. DNase II had no activity, and its k cat / K m value could not be determined. ..

    Recombinant:

    Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
    Article Snippet: .. To determine the degree to which the HTS DNase I assay is sensitive to different endonucleases, varying concentrations of recombinant DNase I, DNase II, and EndoG were tested by this assay. .. The substrate titration at fixed concentrations of enzymes was performed, and the data are presented in .

    Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
    Article Snippet: The background was subtracted prior to the calculation of DNase I activity. .. The percentage of DNase I activity was calculated using Equation 1: DNase\u00a0I\u00a0activity (%) =\u00a0 (mean\u00a0velocity\u00a0of\u00a0a\u00a0compound/mean\u00a0velocity\u00a0of\u00a0DMSO)\u00a0\u00d7\u00a0100 (1) In similar assays, recombinant murine EndoG (produced in-house) was used at a concentration of 0.14 μM in 0.1 mM MgCl2 , 10 mM Tris-HCl, pH 7.4; and DNase II (Worthington, Lakewood, NJ) (3.32 nM) was tested in 100 mM sodium citrate buffer, pH 5.0. .. For evaluation of the quality of the assay, Z’ values were calculated using Equation 2: Z\u2019 =\u00a01\u00a0\u2212\u00a0(3SDC +\u00a03SDB )/(MC \u00a0\u2212\u00a0MB ) (2) where M = mean value; SD = standard deviation; C = control; and B = background.

    other:

    Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
    Article Snippet: DNase II: Genes Enzymes Function.

    Incubation:

    Article Title: Use of rhodizonic acid for rapid detection of root border cell trapping of lead and reversal of trapping with DNaseUse of rhodizonic acid for rapid detection of root border cell trapping of lead and reversal of trapping with DNase
    Article Snippet: .. In addition, border cells incubated in Pb alone for 1 h, then treated with 2 μL of DNase II for 20 min and stained with RA revealed less Pb trapping than border cell samples incubated in Pb alone (Fig. E, F). ..

    Staining:

    Article Title: Use of rhodizonic acid for rapid detection of root border cell trapping of lead and reversal of trapping with DNaseUse of rhodizonic acid for rapid detection of root border cell trapping of lead and reversal of trapping with DNase
    Article Snippet: .. In addition, border cells incubated in Pb alone for 1 h, then treated with 2 μL of DNase II for 20 min and stained with RA revealed less Pb trapping than border cell samples incubated in Pb alone (Fig. E, F). ..

    Produced:

    Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
    Article Snippet: The background was subtracted prior to the calculation of DNase I activity. .. The percentage of DNase I activity was calculated using Equation 1: DNase\u00a0I\u00a0activity (%) =\u00a0 (mean\u00a0velocity\u00a0of\u00a0a\u00a0compound/mean\u00a0velocity\u00a0of\u00a0DMSO)\u00a0\u00d7\u00a0100 (1) In similar assays, recombinant murine EndoG (produced in-house) was used at a concentration of 0.14 μM in 0.1 mM MgCl2 , 10 mM Tris-HCl, pH 7.4; and DNase II (Worthington, Lakewood, NJ) (3.32 nM) was tested in 100 mM sodium citrate buffer, pH 5.0. .. For evaluation of the quality of the assay, Z’ values were calculated using Equation 2: Z\u2019 =\u00a01\u00a0\u2212\u00a0(3SDC +\u00a03SDB )/(MC \u00a0\u2212\u00a0MB ) (2) where M = mean value; SD = standard deviation; C = control; and B = background.

    Concentration Assay:

    Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
    Article Snippet: The background was subtracted prior to the calculation of DNase I activity. .. The percentage of DNase I activity was calculated using Equation 1: DNase\u00a0I\u00a0activity (%) =\u00a0 (mean\u00a0velocity\u00a0of\u00a0a\u00a0compound/mean\u00a0velocity\u00a0of\u00a0DMSO)\u00a0\u00d7\u00a0100 (1) In similar assays, recombinant murine EndoG (produced in-house) was used at a concentration of 0.14 μM in 0.1 mM MgCl2 , 10 mM Tris-HCl, pH 7.4; and DNase II (Worthington, Lakewood, NJ) (3.32 nM) was tested in 100 mM sodium citrate buffer, pH 5.0. .. For evaluation of the quality of the assay, Z’ values were calculated using Equation 2: Z\u2019 =\u00a01\u00a0\u2212\u00a0(3SDC +\u00a03SDB )/(MC \u00a0\u2212\u00a0MB ) (2) where M = mean value; SD = standard deviation; C = control; and B = background.

    In Vitro:

    Article Title: Inhibition of the Complement Alternative Pathway by Chemically Modified DNA Aptamers That Bind with Picomolar Affinity to Factor B
    Article Snippet: DNA aptamers with modified nucleosides have been shown to possess increased resistance to nucleases in serum when compared with native DNA of the same sequence ( , ). .. Consistent with these observations, we found that in vitro stability of SL1100 against 90% (v/v) NHS, 0.2 U/ml human rDNase I, or 14 U/ml porcine DNase II was increased by 14-, 17-, and 6-fold, respectively, when compared with a version of SL1100 in which all seven 2NEdU residues were replaced with natural dT residues ( ). ..

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  • 94
    Worthington Biochemical dnase ii
    Dnase Ii, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase ii/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase ii - by Bioz Stars, 2021-07
    94/100 stars
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