mnase  (Worthington Biochemical)


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    Worthington Biochemical mnase
    Mnase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mnase/product/Worthington Biochemical
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mnase - by Bioz Stars, 2021-07
    95/100 stars

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    Article Title: Flavored e-cigarette liquids and cinnamaldehyde impair respiratory innate immune cell function
    Article Snippet: Chromatin extruded by activated neutrophils was digested with 500 mU/ml micrococcal nuclease (Worthington Biochemical, Lakewood, NJ).

    Article Title: A Compendium of Nucleosome and Transcript Profiles Reveals Determinants of Chromatin Architecture and Transcription
    Article Snippet: Micrococcal nuclease (MNase; Worthington) dissolved in water at 0.1 U/µL stock was added to the tubes at concentrations of 0, 25, 50, 75, 100, and 150 U per sample.

    Article Title: Functional Oligomerization of the Saccharomyces cerevisiae Isoprenylcysteine Carboxyl Methyltransferase, Ste14p
    Article Snippet: Micrococcal nuclease was purchased from Worthington Biochemical Corp. (Lakewood, NJ), and aprotinin was purchased from MP Biomedical (Irvine, CA).

    Article Title: The KRAB zinc finger protein ZFP809 is required to initiate epigenetic silencing of endogenous retroviruses
    Article Snippet: Briefly, ESC and MEF chromatin was digested for 9 or 12 min, respectively, to obtain predominantly mononucleosomal fragments using 150 U/mL micrococcal nuclease (Worthington Biochemicals).

    Article Title: Transcription factor binding dynamics during human ESC differentiation
    Article Snippet: Nuclei were then digested using MNase (Worthington, LS004797) as done in .

    Article Title: The histone variant H2A.Z in yeast is almost exclusively incorporated into the +1 nucleosome in the direction of transcription
    Article Snippet: Catalog # LS004797) at 25, 50, 75, and 100 U/ml for 10 min at 37°C.

    Article Title: Chromatin context and ncRNA highlight targets of MeCP2 in brain
    Article Snippet: Each washed pellet was re-suspended in 400 µL of MNase digestion buffer and rested on ice, before adding 250 units micrococcal nuclease (Worthington) and allowing digestion for 8 min. Digestion was stopped by addition of 16 µL MNase stop solution (125 mM EDTA, 125 mM EGTA).

    Binding Assay:

    Article Title: Gene Looping Is Conferred by Activator-dependent Interaction of Transcription Initiation and Termination Machineries *
    Article Snippet: .. One sample of the cell lysate, grown under induced conditions, was pretreated with 10 units of micrococcal nuclease (Worthington Labs) for 30 min at 37 °C prior to binding. .. A 1-ml aliquot of the micrococcal nuclease-treated cell lysate was checked for complete digestion of chromatin by running on 1.5% agarose gel following deproteinization and decross-linking of samples.

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  • 95
    Worthington Biochemical mnase
    Mnase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mnase/product/Worthington Biochemical
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mnase - by Bioz Stars, 2021-07
    95/100 stars
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