neonatal rat ventricular myocyte monolayers nrvms  (Worthington Biochemical)


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  • 93
    Name:
    HBSS Solution
    Description:
    Sterile calcium and magnesium free Hank s balanced salt solution CMFHBSS pH 7 4 as supplied in the NCIS kit 1 x 500 ml
    Catalog Number:
    lk003210
    Price:
    54
    Size:
    1 ea
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    Structured Review

    Worthington Biochemical neonatal rat ventricular myocyte monolayers nrvms
    Sterile calcium and magnesium free Hank s balanced salt solution CMFHBSS pH 7 4 as supplied in the NCIS kit 1 x 500 ml
    https://www.bioz.com/result/neonatal rat ventricular myocyte monolayers nrvms/product/Worthington Biochemical
    Average 93 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    neonatal rat ventricular myocyte monolayers nrvms - by Bioz Stars, 2020-08
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: Nogo-C regulates post myocardial infarction fibrosis through the interaction with ER Ca2+ leakage channel Sec61α in mouse hearts
    Article Snippet: .. Isolation and culture of rat neonatal cardiac fibroblasts Ventricles of Sprague–Dawley rats postnatal 1–2 days were cut off and digested in HBSS solution (KCl 0.4 g/L, KH2 PO4 0.06 g/L, NaHCO3 0.35 g/L, NaCl 8 g/L, Na2 HPO4 12H2 O 0.12 g/L, glucose 1 g/L, pH 7.4) containing 0.05% type II collagenase (Worthington, USA) and 0.1% trypsin (Invitrogen, USA). ..

    Incubation:

    Article Title: Impaired intestinal afferent nerve satiety signalling and vagal afferent excitability in diet induced obesity in the mouse
    Article Snippet: .. This was followed by a 10 min incubation in a HBSS solution containing 1 mg ml−1 collagenase type II (Worthington) and 4 mg ml−1 dispase II (Boehringer-Mannheim, Gaithersburg, MD, USA). ..

    Article Title: PKC θ activity maintains normal quantal size in chromaffin cells
    Article Snippet: .. After several washes with HBSS, the tissue was incubated with Ca2+ -free collagenase IA (250 units/mL, Worthington Biochemical Corp., Lakewood, NJ, USA) in HBSS for 30 min at 30°C while stirring. .. The tissue was rinsed three times and triturated gently in HBSS containing 1% heat-inactivated bovine serum albumin and 0.02% Dnase I. Dissociated cells were collected at 1000 g for 3 min and resuspended in culture medium (Dulbecco’s modified Eagle’s medium, 10% fetal bovine serum, 2 mM glutamine, 50 units/mL penicillin, and 50 μg/mL streptomycin).

    Article Title: Brain-Derived Neurotrophic Factor Differentially Regulates Excitatory and Inhibitory Synaptic Transmission in Hippocampal Cultures
    Article Snippet: .. Each hippocampus was diced and incubated at 37°C for 45 min in HBSS containing 20 U/ml papain (Worthington, Freehold, NJ), 0.5 m m EDTA, 1.5 m m CaCl2 , and 10 m m HEPES (Sigma, St. Louis, MO). ..

    Article Title: T-bet Expression by Foxp3+ T Regulatory Cells is Not Essential for Their Suppressive Function in CNS Autoimmune Disease or Colitis
    Article Snippet: .. Briefly, the intestinal epithelial layer was removed by incubation in HBSS 2 mM EDTA for 30 min, and the remaining tissue digested with 1.25 mg/ml collagenase-4 (Worthington) and 30 μg/ml DNase-1 (Roche) in culture medium and disaggregated with a gentle MACS dissociator (Miltenyi). .. Retrieved lymphoid cells were stained and analyzed by flow cytometry.

    Magnetic Cell Separation:

    Article Title: T-bet Expression by Foxp3+ T Regulatory Cells is Not Essential for Their Suppressive Function in CNS Autoimmune Disease or Colitis
    Article Snippet: .. Briefly, the intestinal epithelial layer was removed by incubation in HBSS 2 mM EDTA for 30 min, and the remaining tissue digested with 1.25 mg/ml collagenase-4 (Worthington) and 30 μg/ml DNase-1 (Roche) in culture medium and disaggregated with a gentle MACS dissociator (Miltenyi). .. Retrieved lymphoid cells were stained and analyzed by flow cytometry.

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  • 93
    Worthington Biochemical glu c protease
    Phosphorylation sites identified by each approach. (a) Total number of distinct phosphorylation sites by method. Each experimental approach identified a larger number of phosphorylation sites than <t>Glu-C</t> only workflows. (b) Comparison of unique phosphorylation
    Glu C Protease, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glu c protease/product/Worthington Biochemical
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glu c protease - by Bioz Stars, 2020-08
    93/100 stars
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    93
    Worthington Biochemical neonatal rat ventricular myocyte monolayers nrvms
    Phosphorylation sites identified by each approach. (a) Total number of distinct phosphorylation sites by method. Each experimental approach identified a larger number of phosphorylation sites than <t>Glu-C</t> only workflows. (b) Comparison of unique phosphorylation
    Neonatal Rat Ventricular Myocyte Monolayers Nrvms, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neonatal rat ventricular myocyte monolayers nrvms/product/Worthington Biochemical
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neonatal rat ventricular myocyte monolayers nrvms - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    89
    Worthington Biochemical old neonatal wistar rats
    Phosphorylation sites identified by each approach. (a) Total number of distinct phosphorylation sites by method. Each experimental approach identified a larger number of phosphorylation sites than <t>Glu-C</t> only workflows. (b) Comparison of unique phosphorylation
    Old Neonatal Wistar Rats, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/old neonatal wistar rats/product/Worthington Biochemical
    Average 89 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    old neonatal wistar rats - by Bioz Stars, 2020-08
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    Image Search Results


    Phosphorylation sites identified by each approach. (a) Total number of distinct phosphorylation sites by method. Each experimental approach identified a larger number of phosphorylation sites than Glu-C only workflows. (b) Comparison of unique phosphorylation

    Journal: Analytical and bioanalytical chemistry

    Article Title: Increasing phosphoproteomic coverage through sequential digestion by complementary proteases

    doi: 10.1007/s00216-011-5466-5

    Figure Lengend Snippet: Phosphorylation sites identified by each approach. (a) Total number of distinct phosphorylation sites by method. Each experimental approach identified a larger number of phosphorylation sites than Glu-C only workflows. (b) Comparison of unique phosphorylation

    Article Snippet: Briefly, a single, large pool of NCI-H23 cells were lysed in 8M urea, and their proteins were digested using Glu-C protease, desalted, and lyophilized in three equal (5mg) aliquots.

    Techniques:

    Experimental design and analysis of sequential digestion workflows. (a) Schematic diagram of the three sequential digestion approaches. GSPT closely resembles the traditional workflow with substitution of Glu-C for initial digestion and trypsin digestion

    Journal: Analytical and bioanalytical chemistry

    Article Title: Increasing phosphoproteomic coverage through sequential digestion by complementary proteases

    doi: 10.1007/s00216-011-5466-5

    Figure Lengend Snippet: Experimental design and analysis of sequential digestion workflows. (a) Schematic diagram of the three sequential digestion approaches. GSPT closely resembles the traditional workflow with substitution of Glu-C for initial digestion and trypsin digestion

    Article Snippet: Briefly, a single, large pool of NCI-H23 cells were lysed in 8M urea, and their proteins were digested using Glu-C protease, desalted, and lyophilized in three equal (5mg) aliquots.

    Techniques:

    Evaluation of a Glu-C only based phosphoproteomic workflow. (a) In the traditional phosphoproteomic workflow, proteins are obtained from cell lysates and proteolytically digested with trypsin. The resultant peptides are separated into fractions via strong

    Journal: Analytical and bioanalytical chemistry

    Article Title: Increasing phosphoproteomic coverage through sequential digestion by complementary proteases

    doi: 10.1007/s00216-011-5466-5

    Figure Lengend Snippet: Evaluation of a Glu-C only based phosphoproteomic workflow. (a) In the traditional phosphoproteomic workflow, proteins are obtained from cell lysates and proteolytically digested with trypsin. The resultant peptides are separated into fractions via strong

    Article Snippet: Briefly, a single, large pool of NCI-H23 cells were lysed in 8M urea, and their proteins were digested using Glu-C protease, desalted, and lyophilized in three equal (5mg) aliquots.

    Techniques: