neonatal rat ventricular myocyte monolayers nrvms  (Worthington Biochemical)


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    Structured Review

    Worthington Biochemical neonatal rat ventricular myocyte monolayers nrvms
    Neonatal Rat Ventricular Myocyte Monolayers Nrvms, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neonatal rat ventricular myocyte monolayers nrvms/product/Worthington Biochemical
    Average 93 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    neonatal rat ventricular myocyte monolayers nrvms - by Bioz Stars, 2020-05
    93/100 stars

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    Related Articles

    Patch Clamp:

    Article Title: Stilbene derivative as a photosensitive compound to control the excitability of neonatal rat cardiomyocytes
    Article Snippet: .. Preparation of neonatal rat ventricular cardiomyocyte (NRVM) for patch clamp for optical mapping Hearts were isolated from neonatal 1–2-day-old Wistar rats using a two-day protocol of Neonatal Cardiomyocyte Isolation System of Worthington Biochemical Corporation [ ] with some modifications. ..

    Isolation:

    Article Title: Three-Dimensional Culture Alters Primary Cardiac Cell Phenotype
    Article Snippet: .. NRCVCs were isolated using Neonatal Cardiomyocyte Isolation System kits (Worthington Biochemical, Lakewood, NJ) as in previous studies by our group. .. Viable cells were enumerated by Trypan-blue exclusion (Invitrogen, Carlsbad, CA).

    Article Title: Single Cell Transcriptomics Reconstructs Fate Conversion from Fibroblast to Cardiomyocyte
    Article Snippet: .. Neonatal CMs were isolated using the neonatal cardiomyocytes isolation system (Worthington Biochemical Corporation) except that all enzymes were used at a ¼ of the recommended concentration to increase cell viability. .. After a 1.5 hour pre-plating on uncoated surface to remove attached nonmyocytes, the unattached CMs were collected in TRIzol ( > 80% viability by Trypan blue staining).

    Article Title: Control of histone H3 phosphorylation by CaMKIIδ in response to haemodynamic cardiac stress
    Article Snippet: .. Primary neonatal rat cardiomyocytes isolated using the Worthington Neonatal CardioMyocytes System (Worthington, USA) were co-transfected with a GFP-tagged plasmid and siControl (siCt) or siRNA against 14–3–3 (si14–3–3) from Dharmacon (Thermo Scientific, USA) using lipofectamine (Invitrogen). .. 24 h post-transfection, total cell lysates were prepared and analysed by western blotting or immunofluorescence.

    Article Title: Muscle RING finger-1 attenuates IGF-I-dependent cardiomyocyte hypertrophy by inhibiting JNK signaling
    Article Snippet: .. NRVM were isolated using the Worthington Neonatal Cardiomyocyte Isolation System (Worthington Biochemical, Lakewood, NJ) according to the manufacturer's protocol. ..

    Article Title: Docosahexaenoic acid inhibits protein kinase C translocation/activation and cardiac hypertrophy in rat cardiomyocytes
    Article Snippet: .. Isolation of cardiomyocytes Neonatal cardiomyocytes were obtained using an isolation system from Worthington Biochemical Corporation. ..

    Article Title: Human Myocardial Pericytes: Multipotent Mesodermal Precursors Exhibiting Cardiac Specificity
    Article Snippet: .. Rat neonatal cardiomyocytes were isolated from anaesthetized 3-day-old rat pups using the Worthington rat neonatal cardiomyocyte isolation system per the manufacturer’s protocol (Worthington Biochemical Corp). .. Pericyte/cardiomyocyte co-culture protocol was adopted and modified from previous reports ( , ).

    Article Title: An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses
    Article Snippet: .. Primary cardiomyocytes were isolated from newborn Dahl salt-sensitive rats using the Neonatal Cardiomyocytes Isolation System (Worthington, Lakewood, NJ) according to the manufacturer’s instruction. ..

    Article Title: Stilbene derivative as a photosensitive compound to control the excitability of neonatal rat cardiomyocytes
    Article Snippet: .. Preparation of neonatal rat ventricular cardiomyocyte (NRVM) for patch clamp for optical mapping Hearts were isolated from neonatal 1–2-day-old Wistar rats using a two-day protocol of Neonatal Cardiomyocyte Isolation System of Worthington Biochemical Corporation [ ] with some modifications. ..

    Plasmid Preparation:

    Article Title: Control of histone H3 phosphorylation by CaMKIIδ in response to haemodynamic cardiac stress
    Article Snippet: .. Primary neonatal rat cardiomyocytes isolated using the Worthington Neonatal CardioMyocytes System (Worthington, USA) were co-transfected with a GFP-tagged plasmid and siControl (siCt) or siRNA against 14–3–3 (si14–3–3) from Dharmacon (Thermo Scientific, USA) using lipofectamine (Invitrogen). .. 24 h post-transfection, total cell lysates were prepared and analysed by western blotting or immunofluorescence.

    Concentration Assay:

    Article Title: Single Cell Transcriptomics Reconstructs Fate Conversion from Fibroblast to Cardiomyocyte
    Article Snippet: .. Neonatal CMs were isolated using the neonatal cardiomyocytes isolation system (Worthington Biochemical Corporation) except that all enzymes were used at a ¼ of the recommended concentration to increase cell viability. .. After a 1.5 hour pre-plating on uncoated surface to remove attached nonmyocytes, the unattached CMs were collected in TRIzol ( > 80% viability by Trypan blue staining).