neonatal rat ventricular myocyte monolayers nrvms  (Worthington Biochemical)


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    Name:
    L 15 Media Powder L15NK
    Description:
    Leibovitz L 15 media powder a component of the NCIS kit Reconstitute entire contents of pouch QS to 1 liter with cell culture grade water and 0 22 micron filter Suitable for cell isolation and culture applications
    Catalog Number:
    lk003250
    Price:
    28
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    1 ea
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    Worthington Biochemical neonatal rat ventricular myocyte monolayers nrvms
    Leibovitz L 15 media powder a component of the NCIS kit Reconstitute entire contents of pouch QS to 1 liter with cell culture grade water and 0 22 micron filter Suitable for cell isolation and culture applications
    https://www.bioz.com/result/neonatal rat ventricular myocyte monolayers nrvms/product/Worthington Biochemical
    Average 93 stars, based on 65 article reviews
    Price from $9.99 to $1999.99
    neonatal rat ventricular myocyte monolayers nrvms - by Bioz Stars, 2020-10
    93/100 stars

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    Isolation:

    Article Title: Cyclic AMP and Polyamines Overcome Inhibition by Myelin-Associated Glycoprotein through eIF5A-Mediated Increases in p35 Expression and Activation of Cdk5
    Article Snippet: .. DRG were isolated from P5–P8 Long–Evans rats and incubated in L15 media containing of 0.025% trypsin and 0.15% collagenase type I (Worthington) for 90 min at 37°C. ..

    Incubation:

    Article Title: Impact of Sex Steroid Ablation on Viral, Tumour and Vaccine Responses in Aged Mice
    Article Snippet: .. 3 ml of L15 media containing 1 mg/ml L-(tosylamido-2-phenyl) ethyl chloromethyl ketone-treated trypsin (Worthington) combined with agarose (0.9%) was added and the cultures were incubated at 37°C, 5% CO2 for 3 days. ..

    Article Title: Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording
    Article Snippet: .. Preparation of SCGN cultures Ganglia were incubated for 12–15 minutes at 37 °C in 1 mL L15 media containing 0.1 U/mL collagenase type I (Worthington, Biochemical Corporation). .. L15 was then completely removed and ganglia incubated for 25–30 minutes at 37 °C in 5 mL buffered Ca2+ and Mg2+ -free HBSS (Life Technologies™) buffered with 10 mM Hepes containing 0.05% trypsin (Sigma-Aldrich®).

    Article Title: Cyclic AMP and Polyamines Overcome Inhibition by Myelin-Associated Glycoprotein through eIF5A-Mediated Increases in p35 Expression and Activation of Cdk5
    Article Snippet: .. DRG were isolated from P5–P8 Long–Evans rats and incubated in L15 media containing of 0.025% trypsin and 0.15% collagenase type I (Worthington) for 90 min at 37°C. ..

    other:

    Article Title: Transcriptome Profiling of Layer 5 Intratelencephalic Projection Neurons From the Mature Mouse Motor Cortex
    Article Snippet: Cell Dissociation Dissected samples were diced finely in L15 complete media and transferred to a 2 mL eppendorf tube.

    Magnetic Cell Separation:

    Article Title: Transcriptome Profiling of Layer 5 Intratelencephalic Projection Neurons From the Mature Mouse Motor Cortex
    Article Snippet: .. After tissue settled, L15 media was removed and replaced with digestion media; 12 U/mL papain (Worthington Biochemical Corporation, Lakewood, NJ, USA) and 1 U/mL DNase I (Invitrogen, New Zealand) in L15 complete media, and rotated on MACS mixer (Miltenyi Biotec, Sunnyvale, CA, USA) for 15 min at 37°C. .. Digestion media was then replaced with blocking/trituration solution (2% B27, 1 U/mL DNase I; Invitrogen, New Zealand) and incubated for 10 min at 37°C with 5% CO2 .

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    Worthington Biochemical glu c protease
    Phosphorylation sites identified by each approach. (a) Total number of distinct phosphorylation sites by method. Each experimental approach identified a larger number of phosphorylation sites than <t>Glu-C</t> only workflows. (b) Comparison of unique phosphorylation
    Glu C Protease, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glu c protease/product/Worthington Biochemical
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glu c protease - by Bioz Stars, 2020-10
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    93
    Worthington Biochemical neonatal rat ventricular myocyte monolayers nrvms
    Phosphorylation sites identified by each approach. (a) Total number of distinct phosphorylation sites by method. Each experimental approach identified a larger number of phosphorylation sites than <t>Glu-C</t> only workflows. (b) Comparison of unique phosphorylation
    Neonatal Rat Ventricular Myocyte Monolayers Nrvms, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neonatal rat ventricular myocyte monolayers nrvms/product/Worthington Biochemical
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neonatal rat ventricular myocyte monolayers nrvms - by Bioz Stars, 2020-10
    93/100 stars
      Buy from Supplier

    89
    Worthington Biochemical old neonatal wistar rats
    Phosphorylation sites identified by each approach. (a) Total number of distinct phosphorylation sites by method. Each experimental approach identified a larger number of phosphorylation sites than <t>Glu-C</t> only workflows. (b) Comparison of unique phosphorylation
    Old Neonatal Wistar Rats, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/old neonatal wistar rats/product/Worthington Biochemical
    Average 89 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Phosphorylation sites identified by each approach. (a) Total number of distinct phosphorylation sites by method. Each experimental approach identified a larger number of phosphorylation sites than Glu-C only workflows. (b) Comparison of unique phosphorylation

    Journal: Analytical and bioanalytical chemistry

    Article Title: Increasing phosphoproteomic coverage through sequential digestion by complementary proteases

    doi: 10.1007/s00216-011-5466-5

    Figure Lengend Snippet: Phosphorylation sites identified by each approach. (a) Total number of distinct phosphorylation sites by method. Each experimental approach identified a larger number of phosphorylation sites than Glu-C only workflows. (b) Comparison of unique phosphorylation

    Article Snippet: Briefly, a single, large pool of NCI-H23 cells were lysed in 8M urea, and their proteins were digested using Glu-C protease, desalted, and lyophilized in three equal (5mg) aliquots.

    Techniques:

    Experimental design and analysis of sequential digestion workflows. (a) Schematic diagram of the three sequential digestion approaches. GSPT closely resembles the traditional workflow with substitution of Glu-C for initial digestion and trypsin digestion

    Journal: Analytical and bioanalytical chemistry

    Article Title: Increasing phosphoproteomic coverage through sequential digestion by complementary proteases

    doi: 10.1007/s00216-011-5466-5

    Figure Lengend Snippet: Experimental design and analysis of sequential digestion workflows. (a) Schematic diagram of the three sequential digestion approaches. GSPT closely resembles the traditional workflow with substitution of Glu-C for initial digestion and trypsin digestion

    Article Snippet: Briefly, a single, large pool of NCI-H23 cells were lysed in 8M urea, and their proteins were digested using Glu-C protease, desalted, and lyophilized in three equal (5mg) aliquots.

    Techniques:

    Evaluation of a Glu-C only based phosphoproteomic workflow. (a) In the traditional phosphoproteomic workflow, proteins are obtained from cell lysates and proteolytically digested with trypsin. The resultant peptides are separated into fractions via strong

    Journal: Analytical and bioanalytical chemistry

    Article Title: Increasing phosphoproteomic coverage through sequential digestion by complementary proteases

    doi: 10.1007/s00216-011-5466-5

    Figure Lengend Snippet: Evaluation of a Glu-C only based phosphoproteomic workflow. (a) In the traditional phosphoproteomic workflow, proteins are obtained from cell lysates and proteolytically digested with trypsin. The resultant peptides are separated into fractions via strong

    Article Snippet: Briefly, a single, large pool of NCI-H23 cells were lysed in 8M urea, and their proteins were digested using Glu-C protease, desalted, and lyophilized in three equal (5mg) aliquots.

    Techniques: