mcgovern a  (Worthington Biochemical)


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    Structured Review

    Worthington Biochemical mcgovern a
    Mcgovern A, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 91/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mcgovern a/product/Worthington Biochemical
    Average 91 stars, based on 207 article reviews
    Price from $9.99 to $1999.99
    mcgovern a - by Bioz Stars, 2021-01
    91/100 stars

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    Incubation:

    Article Title: Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis
    Article Snippet: .. Four microliter 0.1 M ammonium acetate pH 5.3 and 8 U Nuclease P1 (Sigma-Aldrich) were added, and samples were incubated at 45°C for 2 h. Four microliters of 0.1 M ammonium bicarbonate and 0.1 U Phosphodiesterase I (Worthington Biochemical, Lakewood, NJ, USA) were added, and samples were incubated at 37°C for 2 h. Five units Antarctic phosphatase (New England Biolabs) were added, and samples were incubated at 37°C for 1 h. .. Liquid chromatography and ESI-MS analysis Nucleosides were separated, at room temperature, on a Hitachi HPLC system (L-7100 pump) with UV detection at 260 nm (L-7400 UV detector).

    Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate
    Article Snippet: .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and phosphodiesterase I (0.03 U/μl, Worthington Biochemical Corp.) in alkaline phosphatase buffer was incubated at 37°C for 1 h. The reaction mixture was heated at 90°C for 3 min, cooled to 0°C for 3 min and analyzed by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA for analysis or HCOONH4 for purification, solvent B: MeCN, linear gradient: B 5–40%/ 30 min, flow rate: 1.0 ml/min). .. The alkylated thymidine dT* obtained by enzymatic hydrolysis of the alkylated ODN 5 and ODN 6 was dissolved in DMSO-d6 containing 10 μM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), the final concentration being 400 μM.

    other:

    Article Title: Mono(ADP-ribosyl)ation of 2?-deoxyguanosine residue in DNA by an apoptosis-inducing protein, pierisin-1, from cabbage butterfly
    Article Snippet: Micrococcal nuclease and phosphodiesterase I ( Crotalus adamanteus venom) and II (bovine spleen) were obtained from Worthington. β-[ Adenylate -32 P]NAD ([32 P]NAD) was purchased from NEN.

    Flow Cytometry:

    Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate
    Article Snippet: .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and phosphodiesterase I (0.03 U/μl, Worthington Biochemical Corp.) in alkaline phosphatase buffer was incubated at 37°C for 1 h. The reaction mixture was heated at 90°C for 3 min, cooled to 0°C for 3 min and analyzed by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA for analysis or HCOONH4 for purification, solvent B: MeCN, linear gradient: B 5–40%/ 30 min, flow rate: 1.0 ml/min). .. The alkylated thymidine dT* obtained by enzymatic hydrolysis of the alkylated ODN 5 and ODN 6 was dissolved in DMSO-d6 containing 10 μM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), the final concentration being 400 μM.

    Purification:

    Article Title: Selective alkylation of T–T mismatched DNA using vinyldiaminotriazine–acridine conjugate
    Article Snippet: .. A solution (100 μl) of the alkylated ODN 5 or ODN 6 (100 μM), alkaline phosphatase (0.03 U/μl, Takara Bio Inc.) and phosphodiesterase I (0.03 U/μl, Worthington Biochemical Corp.) in alkaline phosphatase buffer was incubated at 37°C for 1 h. The reaction mixture was heated at 90°C for 3 min, cooled to 0°C for 3 min and analyzed by RP-HPLC (CAPCELL PAL MG-II, Shiseido, 4.6 × 250 mm, solvent A: 50 mM TEAA for analysis or HCOONH4 for purification, solvent B: MeCN, linear gradient: B 5–40%/ 30 min, flow rate: 1.0 ml/min). .. The alkylated thymidine dT* obtained by enzymatic hydrolysis of the alkylated ODN 5 and ODN 6 was dissolved in DMSO-d6 containing 10 μM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), the final concentration being 400 μM.

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