vector psnap tag  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Name:
    pSNAPf Vector
    Description:
    pSNAPf Vector 20 ug
    Catalog Number:
    n9183s
    Price:
    166
    Size:
    20 ug
    Category:
    Vectors Plasmids
    Buy from Supplier


    Structured Review

    New England Biolabs vector psnap tag
    pSNAPf Vector
    pSNAPf Vector 20 ug
    https://www.bioz.com/result/vector psnap tag/product/New England Biolabs
    Average 95 stars, based on 70 article reviews
    Price from $9.99 to $1999.99
    vector psnap tag - by Bioz Stars, 2020-05
    95/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Quantifying Transcription Factor Binding Dynamics at the Single-molecule Level in Live Cells
    Article Snippet: .. This was generated by PCR amplification from HaloTag–GR and sub cloned into the pSNAPf (N9183S, New England Biolabs, Ipswich, MA, USA) backbone with NheI and AgeI sites. .. The CLIP-tag–GR expresses the rat GR with CLIP-tag fused in the C-terminal domain under the CMV promoter.

    Amplification:

    Article Title: Quantifying Transcription Factor Binding Dynamics at the Single-molecule Level in Live Cells
    Article Snippet: .. This was generated by PCR amplification from HaloTag–GR and sub cloned into the pSNAPf (N9183S, New England Biolabs, Ipswich, MA, USA) backbone with NheI and AgeI sites. .. The CLIP-tag–GR expresses the rat GR with CLIP-tag fused in the C-terminal domain under the CMV promoter.

    Sequencing:

    Article Title: Two-color STED microscopy in living cells
    Article Snippet: .. A linker encoding the signal sequence of EGFR was inserted into 5′ MCS of pSNAPf vector (New England Biolabs) using the unique NheI and EcoRI sites. .. Subsequently, the coding sequence of mature EGFR (GeneCopoeia) was amplified by PCR and subcloned into the plasmid pSNAPf using the unique SbfI and NotI sites creating pSNAPf -EGFR.

    Article Title: The Arabidopsis apyrase AtAPY1 is localized in the Golgi instead of the extracellular space
    Article Snippet: .. The vector pSNAP-tag(m) (New England Biolabs) was used as the PCR template for the SNAP -tag sequence. .. The primer pair for the amplification produced a 531-bp product and contained the following SNAP -specific sequences: 5’-GACTGCGAAATGAAGCGCA-3’ (SNAPlokattB3F; forward) and 5’-TTAAGGCTTGCCCAGTCTGTG-3’ (SNAPlokattB2R; reverse).

    Generated:

    Article Title: ANKRD13C Acts as a Molecular Chaperone for G Protein-coupled Receptors *
    Article Snippet: .. PCR products were inserted in the pcDNA3 vector (Invitrogen) or pSNAP-tag vector (New England Biolabs). cDNA fragments coding for the C terminus of DP were generated by PCR using the Phusion High-Fidelity PCR system (New England Biolabs) and introduced into the pGEX-4T vector (GE Healthcare). .. To produce the His-myc-ANKRD13C protein, the cDNA coding for full-length ANKRD13C was amplified by PCR and inserted into the pRSETa expression vector (Invitrogen).

    Article Title: Quantifying Transcription Factor Binding Dynamics at the Single-molecule Level in Live Cells
    Article Snippet: .. This was generated by PCR amplification from HaloTag–GR and sub cloned into the pSNAPf (N9183S, New England Biolabs, Ipswich, MA, USA) backbone with NheI and AgeI sites. .. The CLIP-tag–GR expresses the rat GR with CLIP-tag fused in the C-terminal domain under the CMV promoter.

    Polymerase Chain Reaction:

    Article Title: Direct Profiling the Post-Translational Modification Codes of a Single Protein Immobilized on a Surface Using Cu-free Click Chemistry
    Article Snippet: .. The SNAP-tag DNA fragment was prepared by PCR using vector pSNAPf (NEB) as the template and the following primers: forward ( AscI ): 5′- GGCGCGCC ACATCATCACCATCACCAT ATGGACAAAGACTGCGAAATG-3′; reverse ( SacII ): 5′-TCC CCGCGG CCCTCCACTCCCACT ACCCAGCCCAGGCTTGCCCAG-3′. .. O 6 -[4-(Aminomethyl)benzyl]guanine (Matrix Scientific, 2.7 mg, 0.01 mmol) was suspended in dry DMF (0.7 mL) under an argon atmosphere, and trimethylamine (10 μL) and DBCO-PEG13 -NHS (Click Chemistry Tools, 10 mg, 0.01 mmol) in dry DMF (0.3 mL) were added.

    Article Title: ANKRD13C Acts as a Molecular Chaperone for G Protein-coupled Receptors *
    Article Snippet: .. PCR products were inserted in the pcDNA3 vector (Invitrogen) or pSNAP-tag vector (New England Biolabs). cDNA fragments coding for the C terminus of DP were generated by PCR using the Phusion High-Fidelity PCR system (New England Biolabs) and introduced into the pGEX-4T vector (GE Healthcare). .. To produce the His-myc-ANKRD13C protein, the cDNA coding for full-length ANKRD13C was amplified by PCR and inserted into the pRSETa expression vector (Invitrogen).

    Article Title: The Arabidopsis apyrase AtAPY1 is localized in the Golgi instead of the extracellular space
    Article Snippet: .. The vector pSNAP-tag(m) (New England Biolabs) was used as the PCR template for the SNAP -tag sequence. .. The primer pair for the amplification produced a 531-bp product and contained the following SNAP -specific sequences: 5’-GACTGCGAAATGAAGCGCA-3’ (SNAPlokattB3F; forward) and 5’-TTAAGGCTTGCCCAGTCTGTG-3’ (SNAPlokattB2R; reverse).

    Article Title: Quantifying Transcription Factor Binding Dynamics at the Single-molecule Level in Live Cells
    Article Snippet: .. This was generated by PCR amplification from HaloTag–GR and sub cloned into the pSNAPf (N9183S, New England Biolabs, Ipswich, MA, USA) backbone with NheI and AgeI sites. .. The CLIP-tag–GR expresses the rat GR with CLIP-tag fused in the C-terminal domain under the CMV promoter.

    Plasmid Preparation:

    Article Title: Two-color STED microscopy in living cells
    Article Snippet: .. A linker encoding the signal sequence of EGFR was inserted into 5′ MCS of pSNAPf vector (New England Biolabs) using the unique NheI and EcoRI sites. .. Subsequently, the coding sequence of mature EGFR (GeneCopoeia) was amplified by PCR and subcloned into the plasmid pSNAPf using the unique SbfI and NotI sites creating pSNAPf -EGFR.

    Article Title: Direct Profiling the Post-Translational Modification Codes of a Single Protein Immobilized on a Surface Using Cu-free Click Chemistry
    Article Snippet: .. The SNAP-tag DNA fragment was prepared by PCR using vector pSNAPf (NEB) as the template and the following primers: forward ( AscI ): 5′- GGCGCGCC ACATCATCACCATCACCAT ATGGACAAAGACTGCGAAATG-3′; reverse ( SacII ): 5′-TCC CCGCGG CCCTCCACTCCCACT ACCCAGCCCAGGCTTGCCCAG-3′. .. O 6 -[4-(Aminomethyl)benzyl]guanine (Matrix Scientific, 2.7 mg, 0.01 mmol) was suspended in dry DMF (0.7 mL) under an argon atmosphere, and trimethylamine (10 μL) and DBCO-PEG13 -NHS (Click Chemistry Tools, 10 mg, 0.01 mmol) in dry DMF (0.3 mL) were added.

    Article Title: Two pathways regulate cortical granule translocation to prevent polyspermy in mouse oocytes
    Article Snippet: .. Rab27a was inserted into a pGEMHE–EGFP or pGEMHE–mCherry vector via XhoI and BamHI. pGEMHE–SNAP–Rab11aS25N was obtained from pGEMHE–EGFP–Rab11aS25N by switching EGFP with SNAP from pSNAPf (New England Biolabs) via HindIII-XhoI. ..

    Article Title: ANKRD13C Acts as a Molecular Chaperone for G Protein-coupled Receptors *
    Article Snippet: .. PCR products were inserted in the pcDNA3 vector (Invitrogen) or pSNAP-tag vector (New England Biolabs). cDNA fragments coding for the C terminus of DP were generated by PCR using the Phusion High-Fidelity PCR system (New England Biolabs) and introduced into the pGEX-4T vector (GE Healthcare). .. To produce the His-myc-ANKRD13C protein, the cDNA coding for full-length ANKRD13C was amplified by PCR and inserted into the pRSETa expression vector (Invitrogen).

    Article Title: The Arabidopsis apyrase AtAPY1 is localized in the Golgi instead of the extracellular space
    Article Snippet: .. The vector pSNAP-tag(m) (New England Biolabs) was used as the PCR template for the SNAP -tag sequence. .. The primer pair for the amplification produced a 531-bp product and contained the following SNAP -specific sequences: 5’-GACTGCGAAATGAAGCGCA-3’ (SNAPlokattB3F; forward) and 5’-TTAAGGCTTGCCCAGTCTGTG-3’ (SNAPlokattB2R; reverse).

    Article Title: The Interaction of Natural Selection and GC Skew May Drive the Fast Evolution of a Sand Rat Homeobox Gene
    Article Snippet: .. Mouse Pdx1 was inserted into pSNAPf vector (New England BioLabs) using Bam HI and Xho I restriction sites, and sand rat Pdx1 inserted using Not I and Xho I restriction sites. .. Stop codons were removed to allow expression of Pdx1-SNAP fusion protein.

    Article Title: Rudhira/BCAS3 couples microtubules and intermediate filaments to promote cell migration for angiogenic remodeling
    Article Snippet: .. Rudhira ORF was subcloned from pCMV-RudhFL-FLAG vector into pSNAPf vector (New England Biolabs) using Nhe I-Eco RI sites to obtain Rudhira-SNAP (Supplemental Figure S3B). .. For deletion mutant cloning, the regions to be cloned were PCR amplified from pCMV-RudhFL-FLAG vector and cloned in pCMV-Tag2B vector (Stratagene) ( and ′).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    New England Biolabs psnapf
    Psnapf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psnapf/product/New England Biolabs
    Average 90 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    psnapf - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    Image Search Results