kbp dna ladder  (New England Biolabs)


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    Name:
    1 kb Plus DNA Ladder
    Description:
    2 Log DNA Ladder 500 1000 gel lanes
    Catalog Number:
    n3200l
    Price:
    231
    Size:
    1000 gel lanes
    Category:
    DNA Ladders
    Buy from Supplier


    Structured Review

    New England Biolabs kbp dna ladder
    1 kb Plus DNA Ladder
    2 Log DNA Ladder 500 1000 gel lanes
    https://www.bioz.com/result/kbp dna ladder/product/New England Biolabs
    Average 95 stars, based on 13229 article reviews
    Price from $9.99 to $1999.99
    kbp dna ladder - by Bioz Stars, 2020-05
    95/100 stars

    Images

    1) Product Images from "Sizing femtogram amounts of dsDNA by single-molecule counting"

    Article Title: Sizing femtogram amounts of dsDNA by single-molecule counting

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkv904

    Single-molecule intensity distribution of a 1 kbp DNA ladder. ( A ) intensity distribution histogram of detected molecules (red), fitted Gaussians sum (black) and the residue of the fitting (blue). ( B ) a plot of expected DNA lengths in the ladder sample versus the centers of the fitted Gaussian peaks exhibits linear dependency with R 2 = 0.99932.
    Figure Legend Snippet: Single-molecule intensity distribution of a 1 kbp DNA ladder. ( A ) intensity distribution histogram of detected molecules (red), fitted Gaussians sum (black) and the residue of the fitting (blue). ( B ) a plot of expected DNA lengths in the ladder sample versus the centers of the fitted Gaussian peaks exhibits linear dependency with R 2 = 0.99932.

    Techniques Used:

    2) Product Images from "Sizing femtogram amounts of dsDNA by single-molecule counting"

    Article Title: Sizing femtogram amounts of dsDNA by single-molecule counting

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkv904

    Single-molecule intensity distribution of a 1 kbp DNA ladder. ( A ) intensity distribution histogram of detected molecules (red), fitted Gaussians sum (black) and the residue of the fitting (blue). ( B ) a plot of expected DNA lengths in the ladder sample versus the centers of the fitted Gaussian peaks exhibits linear dependency with R 2 = 0.99932.
    Figure Legend Snippet: Single-molecule intensity distribution of a 1 kbp DNA ladder. ( A ) intensity distribution histogram of detected molecules (red), fitted Gaussians sum (black) and the residue of the fitting (blue). ( B ) a plot of expected DNA lengths in the ladder sample versus the centers of the fitted Gaussian peaks exhibits linear dependency with R 2 = 0.99932.

    Techniques Used:

    3) Product Images from "Regulation of Rad51 Recombinase Presynaptic Filament Assembly via Interactions with the Rad52 Mediator and the Srs2 Anti-recombinase *"

    Article Title: Regulation of Rad51 Recombinase Presynaptic Filament Assembly via Interactions with the Rad52 Mediator and the Srs2 Anti-recombinase *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M109.032953

    Homologous DNA pairing and strand exchange by rad51 mutants. A , scheme of the homologous DNA pairing and strand exchange reaction. Pairing between the circular ϕX174 (+) ssDNA and linear ϕX174 dsDNA yields a joint molecule ( jm ), which
    Figure Legend Snippet: Homologous DNA pairing and strand exchange by rad51 mutants. A , scheme of the homologous DNA pairing and strand exchange reaction. Pairing between the circular ϕX174 (+) ssDNA and linear ϕX174 dsDNA yields a joint molecule ( jm ), which

    Techniques Used:

    4) Product Images from "Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR"

    Article Title: Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR

    Journal: Molecular Therapy. Methods & Clinical Development

    doi: 10.1038/mtm.2016.19

    Generation of free ends for the plasmid inverted terminal repeats (ITRs). ( a ) Schematic representation of the plasmid psub201 and the PvuII and HindIII restriction sites. ( b ) Magnification of the plasmid DNA sequences close to the PvuII digestion sites. pEMBL8(+) plasmid backbone (lower case); AAV2-sub201 viral genome (upper case) and PvuII site (CAG/CTG): underlined. ( c ) Separation of undigested and digested plasmid DNA on a 1% agarose gel; supercoiled and linear DNA ladder were used as electrophoresis standards. ( d ) Plasmid DNA purity and concentration measured by spectrophotometry.
    Figure Legend Snippet: Generation of free ends for the plasmid inverted terminal repeats (ITRs). ( a ) Schematic representation of the plasmid psub201 and the PvuII and HindIII restriction sites. ( b ) Magnification of the plasmid DNA sequences close to the PvuII digestion sites. pEMBL8(+) plasmid backbone (lower case); AAV2-sub201 viral genome (upper case) and PvuII site (CAG/CTG): underlined. ( c ) Separation of undigested and digested plasmid DNA on a 1% agarose gel; supercoiled and linear DNA ladder were used as electrophoresis standards. ( d ) Plasmid DNA purity and concentration measured by spectrophotometry.

    Techniques Used: Plasmid Preparation, CTG Assay, Agarose Gel Electrophoresis, Electrophoresis, Concentration Assay, Spectrophotometry

    Related Articles

    Amplification:

    Article Title: Paraoxonase-1 (PON1) induces metastatic potential and apoptosis escape via its antioxidative function in lung cancer cells
    Article Snippet: .. RNA was treated with DNA I (New England Biolabs, MA). cDNA amplification was performed using the High Capacity Reverse Transcription Kit (Applied Biosystems). .. Real-time (RT)-PCR, and quantitative reverse transcriptase RT-PCR were performed as described previously [ ] to evaluate mRNA levels of genes of interest. qPCR primer sequences were designed using Primer 3 MIT software and sequences were chosen that spanned exon junctions.

    Agarose Gel Electrophoresis:

    Article Title: Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR
    Article Snippet: .. One hundred ng of each supercoiled and linearized plasmids were analyzed on a 1% native agarose gel alongside a 2-log DNA ladder and supercoiled ladder (New England BioLabs Cat N3200S and N0472S, Ipswich, MA) to confirm complete digestion and purity, and to confirm concentration. .. The concentration determined for each of these templates using the nanophotometer was used to calculate the number of copies of DNA present in each series of dilutions.

    Labeling:

    Article Title: Sizing femtogram amounts of dsDNA by single-molecule counting
    Article Snippet: .. RESULTS In order to validate the linear dependency of intercalation intensity on DNA length, 1 ng of a 1 kbp DNA ladder (NEB) was labeled with the intercalating dye YOYO-1 and imaged. ..

    Electrophoresis:

    Article Title: Longitudinal Analyses of Gut Mucosal Microbiotas in Ulcerative Colitis in Relation to Patient Age and Disease Severity and Duration
    Article Snippet: .. Plasmid concentrations were determined by electrophoresis and comparison of band strengths against molecular marker DNA (2-log DNA ladder N3200L; New England BioLabs Ltd., Herts, United Kingdom) and by using a spectrophotometer (Cecil Instruments, Cambridge, United Kingdom) at 260/280 nm. ..

    Concentration Assay:

    Article Title: Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR
    Article Snippet: .. One hundred ng of each supercoiled and linearized plasmids were analyzed on a 1% native agarose gel alongside a 2-log DNA ladder and supercoiled ladder (New England BioLabs Cat N3200S and N0472S, Ipswich, MA) to confirm complete digestion and purity, and to confirm concentration. .. The concentration determined for each of these templates using the nanophotometer was used to calculate the number of copies of DNA present in each series of dilutions.

    Spectrophotometry:

    Article Title: Longitudinal Analyses of Gut Mucosal Microbiotas in Ulcerative Colitis in Relation to Patient Age and Disease Severity and Duration
    Article Snippet: .. Plasmid concentrations were determined by electrophoresis and comparison of band strengths against molecular marker DNA (2-log DNA ladder N3200L; New England BioLabs Ltd., Herts, United Kingdom) and by using a spectrophotometer (Cecil Instruments, Cambridge, United Kingdom) at 260/280 nm. ..

    Marker:

    Article Title: Longitudinal Analyses of Gut Mucosal Microbiotas in Ulcerative Colitis in Relation to Patient Age and Disease Severity and Duration
    Article Snippet: .. Plasmid concentrations were determined by electrophoresis and comparison of band strengths against molecular marker DNA (2-log DNA ladder N3200L; New England BioLabs Ltd., Herts, United Kingdom) and by using a spectrophotometer (Cecil Instruments, Cambridge, United Kingdom) at 260/280 nm. ..

    Plasmid Preparation:

    Article Title: Longitudinal Analyses of Gut Mucosal Microbiotas in Ulcerative Colitis in Relation to Patient Age and Disease Severity and Duration
    Article Snippet: .. Plasmid concentrations were determined by electrophoresis and comparison of band strengths against molecular marker DNA (2-log DNA ladder N3200L; New England BioLabs Ltd., Herts, United Kingdom) and by using a spectrophotometer (Cecil Instruments, Cambridge, United Kingdom) at 260/280 nm. ..

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  • 95
    New England Biolabs kbp dna ladder
    Single-molecule intensity distribution of a 1 <t>kbp</t> <t>DNA</t> ladder. ( A ) intensity distribution histogram of detected molecules (red), fitted Gaussians sum (black) and the residue of the fitting (blue). ( B ) a plot of expected DNA lengths in the ladder sample versus the centers of the fitted Gaussian peaks exhibits linear dependency with R 2 = 0.99932.
    Kbp Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kbp dna ladder/product/New England Biolabs
    Average 95 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    kbp dna ladder - by Bioz Stars, 2020-05
    95/100 stars
      Buy from Supplier

    Image Search Results


    Single-molecule intensity distribution of a 1 kbp DNA ladder. ( A ) intensity distribution histogram of detected molecules (red), fitted Gaussians sum (black) and the residue of the fitting (blue). ( B ) a plot of expected DNA lengths in the ladder sample versus the centers of the fitted Gaussian peaks exhibits linear dependency with R 2 = 0.99932.

    Journal: Nucleic Acids Research

    Article Title: Sizing femtogram amounts of dsDNA by single-molecule counting

    doi: 10.1093/nar/gkv904

    Figure Lengend Snippet: Single-molecule intensity distribution of a 1 kbp DNA ladder. ( A ) intensity distribution histogram of detected molecules (red), fitted Gaussians sum (black) and the residue of the fitting (blue). ( B ) a plot of expected DNA lengths in the ladder sample versus the centers of the fitted Gaussian peaks exhibits linear dependency with R 2 = 0.99932.

    Article Snippet: In order to simulate analysis of an unknown sample, 0.5 ng of 1 kbp DNA ladder (NEB) were mixed with 0.5 ng of reference sample, containing the previously calibrated 3 kb and 7 kb populations.

    Techniques:

    Homologous DNA pairing and strand exchange by rad51 mutants. A , scheme of the homologous DNA pairing and strand exchange reaction. Pairing between the circular ϕX174 (+) ssDNA and linear ϕX174 dsDNA yields a joint molecule ( jm ), which

    Journal: The Journal of Biological Chemistry

    Article Title: Regulation of Rad51 Recombinase Presynaptic Filament Assembly via Interactions with the Rad52 Mediator and the Srs2 Anti-recombinase *

    doi: 10.1074/jbc.M109.032953

    Figure Lengend Snippet: Homologous DNA pairing and strand exchange by rad51 mutants. A , scheme of the homologous DNA pairing and strand exchange reaction. Pairing between the circular ϕX174 (+) ssDNA and linear ϕX174 dsDNA yields a joint molecule ( jm ), which

    Article Snippet: The ϕX174 replicative form I DNA and viral (+) strand DNA were purchased from New England Biolabs.

    Techniques:

    Generation of free ends for the plasmid inverted terminal repeats (ITRs). ( a ) Schematic representation of the plasmid psub201 and the PvuII and HindIII restriction sites. ( b ) Magnification of the plasmid DNA sequences close to the PvuII digestion sites. pEMBL8(+) plasmid backbone (lower case); AAV2-sub201 viral genome (upper case) and PvuII site (CAG/CTG): underlined. ( c ) Separation of undigested and digested plasmid DNA on a 1% agarose gel; supercoiled and linear DNA ladder were used as electrophoresis standards. ( d ) Plasmid DNA purity and concentration measured by spectrophotometry.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR

    doi: 10.1038/mtm.2016.19

    Figure Lengend Snippet: Generation of free ends for the plasmid inverted terminal repeats (ITRs). ( a ) Schematic representation of the plasmid psub201 and the PvuII and HindIII restriction sites. ( b ) Magnification of the plasmid DNA sequences close to the PvuII digestion sites. pEMBL8(+) plasmid backbone (lower case); AAV2-sub201 viral genome (upper case) and PvuII site (CAG/CTG): underlined. ( c ) Separation of undigested and digested plasmid DNA on a 1% agarose gel; supercoiled and linear DNA ladder were used as electrophoresis standards. ( d ) Plasmid DNA purity and concentration measured by spectrophotometry.

    Article Snippet: One hundred ng of each supercoiled and linearized plasmids were analyzed on a 1% native agarose gel alongside a 2-log DNA ladder and supercoiled ladder (New England BioLabs Cat N3200S and N0472S, Ipswich, MA) to confirm complete digestion and purity, and to confirm concentration.

    Techniques: Plasmid Preparation, CTG Assay, Agarose Gel Electrophoresis, Electrophoresis, Concentration Assay, Spectrophotometry