Journal: International Journal of Molecular Sciences

Article Title: A Reliable Approach for Revealing Molecular Targets in Secondary Ion Mass Spectrometry

doi: 10.3390/ijms23094615

Figure Lengend Snippet: Antibodies and gold particle materials applied.

Article Snippet: Atto542 conjugated anti-mouse nanobody , nanoTag , Cat#: N1202-At542-S.

Techniques:

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Comparison of ORF2–7 sequences of the recombinant virus from original sample, KU-N1202 (passage 10) and other five plaque-purified clones (passage 10).

Article Snippet: To determine the full-length genome sequence of the novel strain, KU-N1202, viral RNA was extracted from selected plaque-purified clones (passage 10; P10) using Qiazol Lysis Reagent (Qiagen, MD, USA) according to the manufacturer's instructions.

Techniques: Recombinant, Clone Assay

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Comparisons of genomic sequence identities between porcine reproductive and respiratory syndrome virus KU-N1202 and VR-2332, five Korean PRRSV-2 isolates, and MN184C.

Article Snippet: To determine the full-length genome sequence of the novel strain, KU-N1202, viral RNA was extracted from selected plaque-purified clones (passage 10; P10) using Qiazol Lysis Reagent (Qiagen, MD, USA) according to the manufacturer's instructions.

Techniques: Sequencing

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Alignment of amino acid sequences of porcine reproductive and respiratory syndrome virus KU-N1202 structural proteins. (A) GP2a, (B) GP3, (C) GP4, (D) GP5, (E) M, and (F) N. Red shade, previously identified B-cell epitope; blue shade, previously identified T-cell epitope; green shade, potential glycosylation site.

Article Snippet: To determine the full-length genome sequence of the novel strain, KU-N1202, viral RNA was extracted from selected plaque-purified clones (passage 10; P10) using Qiazol Lysis Reagent (Qiagen, MD, USA) according to the manufacturer's instructions.

Techniques:

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Recombination analyses of porcine reproductive and respiratory syndrome virus KU-N1202. (A) Similarity plot analysis of KU-N1202 (query) compared to CP07–626–2 (blue) and VR-2332 (red). Yellow indicates recombination region between 13,351 and 14,705, which covers ORF5, ORF6, and partial ORF7. Phylogenetic trees of major (B) and minor (C) parental regions. The major parental region of KU-N1202 was found to be related to Korean MN184-like strains, but the minor parental region of KU-N1202 was closely related to VR-2332. The beginning (D) and ending (E) of recombination breakpoints were elucidated. The beginning breaking point (black box) was found to be located in the transcription-regulating sequence (gray shade box) at the 5′ end of sub-genomic RNA 5. Left and right dashes indicate the stop codon of ORF5 and the start codon of ORF6, respectively. The ending breakpoint (black box) was found to be located in the middle region of ORF7.

Article Snippet: To determine the full-length genome sequence of the novel strain, KU-N1202, viral RNA was extracted from selected plaque-purified clones (passage 10; P10) using Qiazol Lysis Reagent (Qiagen, MD, USA) according to the manufacturer's instructions.

Techniques: Sequencing

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Characteristics of pigs infected with porcine reproductive and respiratory syndrome virus KU-N1202. Mean clinical respiratory score (A) and rectal temperature (B) of challenged (•), contact (■), and control groups (▲).

Article Snippet: To determine the full-length genome sequence of the novel strain, KU-N1202, viral RNA was extracted from selected plaque-purified clones (passage 10; P10) using Qiazol Lysis Reagent (Qiagen, MD, USA) according to the manufacturer's instructions.

Techniques: Infection

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Viral loads of porcine reproductive and respiratory syndrome virus KU-N1202 towards pigs. Mean 50% tissue culture infectious doses/ml (log 10 ) of KU-N1202 in serum (A), nasal swab (B), and pen-based oral fluid samples (C).

Article Snippet: To determine the full-length genome sequence of the novel strain, KU-N1202, viral RNA was extracted from selected plaque-purified clones (passage 10; P10) using Qiazol Lysis Reagent (Qiagen, MD, USA) according to the manufacturer's instructions.

Techniques:

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Distribution of porcine reproductive and respiratory syndrome virus KU-N1202 in tissues of infected pigs. Mean 50% tissue culture infectious doses/ml (log 10 ) of KU-N1202 in different tissues from pigs that were euthanized at 3 days post-infection (dpi) (A), 5 dpi (B), and 7 dpi (C).

Article Snippet: To determine the full-length genome sequence of the novel strain, KU-N1202, viral RNA was extracted from selected plaque-purified clones (passage 10; P10) using Qiazol Lysis Reagent (Qiagen, MD, USA) according to the manufacturer's instructions.

Techniques: Infection

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Histopathology of porcine reproductive and respiratory syndrome virus KU-N1202-infected pigs that were euthanized at 7 days post-infection. Lung and tracheobronchial lymph node in the challenged pig (A and D), contact pig (B and E), and control pig (C and F), respectively. x100, H&E staining.

Article Snippet: To determine the full-length genome sequence of the novel strain, KU-N1202, viral RNA was extracted from selected plaque-purified clones (passage 10; P10) using Qiazol Lysis Reagent (Qiagen, MD, USA) according to the manufacturer's instructions.

Techniques: Histopathology, Infection, Staining

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Comparison of ORF2–7 sequences of the recombinant virus from original sample, KU-N1202 (passage 10) and other five plaque-purified clones (passage 10).

Article Snippet: First, Simplot analysis revealed that KU-N1202 is the result of recombination between CP07–626–2-like and VR-2332-like strains circulating in Korea.

Techniques: Recombinant, Clone Assay

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Comparisons of genomic sequence identities between porcine reproductive and respiratory syndrome virus KU-N1202 and VR-2332, five Korean PRRSV-2 isolates, and MN184C.

Article Snippet: First, Simplot analysis revealed that KU-N1202 is the result of recombination between CP07–626–2-like and VR-2332-like strains circulating in Korea.

Techniques: Sequencing

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Alignment of amino acid sequences of porcine reproductive and respiratory syndrome virus KU-N1202 structural proteins. (A) GP2a, (B) GP3, (C) GP4, (D) GP5, (E) M, and (F) N. Red shade, previously identified B-cell epitope; blue shade, previously identified T-cell epitope; green shade, potential glycosylation site.

Article Snippet: First, Simplot analysis revealed that KU-N1202 is the result of recombination between CP07–626–2-like and VR-2332-like strains circulating in Korea.

Techniques:

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Recombination analyses of porcine reproductive and respiratory syndrome virus KU-N1202. (A) Similarity plot analysis of KU-N1202 (query) compared to CP07–626–2 (blue) and VR-2332 (red). Yellow indicates recombination region between 13,351 and 14,705, which covers ORF5, ORF6, and partial ORF7. Phylogenetic trees of major (B) and minor (C) parental regions. The major parental region of KU-N1202 was found to be related to Korean MN184-like strains, but the minor parental region of KU-N1202 was closely related to VR-2332. The beginning (D) and ending (E) of recombination breakpoints were elucidated. The beginning breaking point (black box) was found to be located in the transcription-regulating sequence (gray shade box) at the 5′ end of sub-genomic RNA 5. Left and right dashes indicate the stop codon of ORF5 and the start codon of ORF6, respectively. The ending breakpoint (black box) was found to be located in the middle region of ORF7.

Article Snippet: First, Simplot analysis revealed that KU-N1202 is the result of recombination between CP07–626–2-like and VR-2332-like strains circulating in Korea.

Techniques: Sequencing

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Characteristics of pigs infected with porcine reproductive and respiratory syndrome virus KU-N1202. Mean clinical respiratory score (A) and rectal temperature (B) of challenged (•), contact (■), and control groups (▲).

Article Snippet: First, Simplot analysis revealed that KU-N1202 is the result of recombination between CP07–626–2-like and VR-2332-like strains circulating in Korea.

Techniques: Infection

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Viral loads of porcine reproductive and respiratory syndrome virus KU-N1202 towards pigs. Mean 50% tissue culture infectious doses/ml (log 10 ) of KU-N1202 in serum (A), nasal swab (B), and pen-based oral fluid samples (C).

Article Snippet: First, Simplot analysis revealed that KU-N1202 is the result of recombination between CP07–626–2-like and VR-2332-like strains circulating in Korea.

Techniques:

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Distribution of porcine reproductive and respiratory syndrome virus KU-N1202 in tissues of infected pigs. Mean 50% tissue culture infectious doses/ml (log 10 ) of KU-N1202 in different tissues from pigs that were euthanized at 3 days post-infection (dpi) (A), 5 dpi (B), and 7 dpi (C).

Article Snippet: First, Simplot analysis revealed that KU-N1202 is the result of recombination between CP07–626–2-like and VR-2332-like strains circulating in Korea.

Techniques: Infection

Journal: Virology

Article Title: Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain

doi: 10.1016/j.virol.2019.01.030

Figure Lengend Snippet: Histopathology of porcine reproductive and respiratory syndrome virus KU-N1202-infected pigs that were euthanized at 7 days post-infection. Lung and tracheobronchial lymph node in the challenged pig (A and D), contact pig (B and E), and control pig (C and F), respectively. x100, H&E staining.

Article Snippet: First, Simplot analysis revealed that KU-N1202 is the result of recombination between CP07–626–2-like and VR-2332-like strains circulating in Korea.

Techniques: Histopathology, Infection, Staining