dna ladder  (New England Biolabs)


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    New England Biolabs dna ladder
    Electrophoretic profile of plasmids extracted from isolated strains. a Electrophoresis was performed on 0.8% agarose gels at 75 V for 16 h. As a comparison marker, the Supercoiled <t>DNA</t> <t>Ladder</t> was used. b Estimation of the size of the plasmid, comparing the isoform profile with the isoform profile contained in the supercoiled DNA Ladder
    Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Regulation Transcriptional of Antibiotic Resistance Genes (ARGs) in Bacteria Isolated from WWTP"

    Article Title: Regulation Transcriptional of Antibiotic Resistance Genes (ARGs) in Bacteria Isolated from WWTP

    Journal: Current Microbiology

    doi: 10.1007/s00284-023-03449-z

    Electrophoretic profile of plasmids extracted from isolated strains. a Electrophoresis was performed on 0.8% agarose gels at 75 V for 16 h. As a comparison marker, the Supercoiled DNA Ladder was used. b Estimation of the size of the plasmid, comparing the isoform profile with the isoform profile contained in the supercoiled DNA Ladder
    Figure Legend Snippet: Electrophoretic profile of plasmids extracted from isolated strains. a Electrophoresis was performed on 0.8% agarose gels at 75 V for 16 h. As a comparison marker, the Supercoiled DNA Ladder was used. b Estimation of the size of the plasmid, comparing the isoform profile with the isoform profile contained in the supercoiled DNA Ladder

    Techniques Used: Isolation, Electrophoresis, Marker, Plasmid Preparation

    dna ladder  (New England Biolabs)


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    New England Biolabs dna ladder
    Electrophoretic profile of plasmids extracted from isolated strains. a Electrophoresis was performed on 0.8% agarose gels at 75 V for 16 h. As a comparison marker, the Supercoiled <t>DNA</t> <t>Ladder</t> was used. b Estimation of the size of the plasmid, comparing the isoform profile with the isoform profile contained in the supercoiled DNA Ladder
    Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Regulation Transcriptional of Antibiotic Resistance Genes (ARGs) in Bacteria Isolated from WWTP"

    Article Title: Regulation Transcriptional of Antibiotic Resistance Genes (ARGs) in Bacteria Isolated from WWTP

    Journal: Current Microbiology

    doi: 10.1007/s00284-023-03449-z

    Electrophoretic profile of plasmids extracted from isolated strains. a Electrophoresis was performed on 0.8% agarose gels at 75 V for 16 h. As a comparison marker, the Supercoiled DNA Ladder was used. b Estimation of the size of the plasmid, comparing the isoform profile with the isoform profile contained in the supercoiled DNA Ladder
    Figure Legend Snippet: Electrophoretic profile of plasmids extracted from isolated strains. a Electrophoresis was performed on 0.8% agarose gels at 75 V for 16 h. As a comparison marker, the Supercoiled DNA Ladder was used. b Estimation of the size of the plasmid, comparing the isoform profile with the isoform profile contained in the supercoiled DNA Ladder

    Techniques Used: Isolation, Electrophoresis, Marker, Plasmid Preparation

    bac tracker supercoiled dna ladder  (New England Biolabs)


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    New England Biolabs bac tracker supercoiled dna ladder
    Bac Tracker Supercoiled Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dna ladder  (New England Biolabs)


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    New England Biolabs dna ladder
    Construction of the pORF-hIL-12-ORT plasmid. (a) Construction plan. (b) Annotated plasmid map confirmed by whole plasmid sequencing. c) Restriction analysis confirming the pORF-hIL-12-ORT plasmid construction: The plasmid was cut with Nhe I, Nco I and Hpa I restriction enzymes and its identity was confirmed by positive matching of the simulated pattern of bands created by SnapGene software (left) to the actual pattern on the electrophoretic gel (right). MW: molecular weight, GeneRuler <t>DNA</t> <t>ladder</t> Mix. Lane 1: Nhe I: 2596 bp, 1556 bp, 363 bp; lane 2: Nco I + Hpa I: 2648 bp, 1857 bp; lane 3: Kpn I: 2317 bp, 2198 bp.
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    1) Product Images from "Maintenance and gene electrotransfer efficiency of antibiotic resistance gene-free plasmids encoding mouse, canine and human interleukin-12 orthologues"

    Article Title: Maintenance and gene electrotransfer efficiency of antibiotic resistance gene-free plasmids encoding mouse, canine and human interleukin-12 orthologues

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2022.e08879

    Construction of the pORF-hIL-12-ORT plasmid. (a) Construction plan. (b) Annotated plasmid map confirmed by whole plasmid sequencing. c) Restriction analysis confirming the pORF-hIL-12-ORT plasmid construction: The plasmid was cut with Nhe I, Nco I and Hpa I restriction enzymes and its identity was confirmed by positive matching of the simulated pattern of bands created by SnapGene software (left) to the actual pattern on the electrophoretic gel (right). MW: molecular weight, GeneRuler DNA ladder Mix. Lane 1: Nhe I: 2596 bp, 1556 bp, 363 bp; lane 2: Nco I + Hpa I: 2648 bp, 1857 bp; lane 3: Kpn I: 2317 bp, 2198 bp.
    Figure Legend Snippet: Construction of the pORF-hIL-12-ORT plasmid. (a) Construction plan. (b) Annotated plasmid map confirmed by whole plasmid sequencing. c) Restriction analysis confirming the pORF-hIL-12-ORT plasmid construction: The plasmid was cut with Nhe I, Nco I and Hpa I restriction enzymes and its identity was confirmed by positive matching of the simulated pattern of bands created by SnapGene software (left) to the actual pattern on the electrophoretic gel (right). MW: molecular weight, GeneRuler DNA ladder Mix. Lane 1: Nhe I: 2596 bp, 1556 bp, 363 bp; lane 2: Nco I + Hpa I: 2648 bp, 1857 bp; lane 3: Kpn I: 2317 bp, 2198 bp.

    Techniques Used: Plasmid Preparation, Sequencing, Software, Molecular Weight

    Electrophoretic evaluation of plasmids. (a) Electrophoresis of 10 μL of minipep eluates from first to fifth passage linearized with Not I restriction enzyme. Plasmid lengths: pORF-mIL-12 (p40p35) - 4833 bp, pORF-mIL-12-ORT - 4380 bp, pORF-caIL-12-ORT - 4293 bp, pORF-hIL-12-ORT - 4515 bp. 1% agarose pre-stained with 1× SYBR tm Safe, 100 V, 1 h, LL, linear ladder: GeneRuler DNA ladder mix. Lanes 1–5, passages. (b) Electrophoresis of 200 ng of uncut plasmids isolated from first to fifth passage. 1% agarose, 100 V, 1 h, stained in 1× SYBR tm Gold. SCL, supercoiled ladder: Supercoiled DNA Ladder. SCm, supercoiled monomer. SCd, supercoiled dimer, SCt, supercoiled topoisomers. Lanes 1–5, passages (c) pORF-hIL-12-ORT plasmid incubated with increasing concentrations of SYBR tm Safe (0, 0.5×, 1× and 2×). SCL, supercoiled ladder: Supercoiled DNA Ladder.
    Figure Legend Snippet: Electrophoretic evaluation of plasmids. (a) Electrophoresis of 10 μL of minipep eluates from first to fifth passage linearized with Not I restriction enzyme. Plasmid lengths: pORF-mIL-12 (p40p35) - 4833 bp, pORF-mIL-12-ORT - 4380 bp, pORF-caIL-12-ORT - 4293 bp, pORF-hIL-12-ORT - 4515 bp. 1% agarose pre-stained with 1× SYBR tm Safe, 100 V, 1 h, LL, linear ladder: GeneRuler DNA ladder mix. Lanes 1–5, passages. (b) Electrophoresis of 200 ng of uncut plasmids isolated from first to fifth passage. 1% agarose, 100 V, 1 h, stained in 1× SYBR tm Gold. SCL, supercoiled ladder: Supercoiled DNA Ladder. SCm, supercoiled monomer. SCd, supercoiled dimer, SCt, supercoiled topoisomers. Lanes 1–5, passages (c) pORF-hIL-12-ORT plasmid incubated with increasing concentrations of SYBR tm Safe (0, 0.5×, 1× and 2×). SCL, supercoiled ladder: Supercoiled DNA Ladder.

    Techniques Used: Electrophoresis, Plasmid Preparation, Staining, Isolation, Incubation

    dna ladder  (New England Biolabs)


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    2 log dna ladder  (New England Biolabs)


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    New England Biolabs 2 log dna ladder
    2 Log Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dna ladder  (New England Biolabs)


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    New England Biolabs dna ladder
    Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dna ladder  (New England Biolabs)


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    New England Biolabs dna ladder
    Construction and confirmation of the p21-mIL-12-ORT plasmid. ( a ) Cloning plan created using the SnapGene software: the expression cassette carrying the murine IL-12 sequence under the EF1/HTLV promoter was cut out of the pORF-mIL-12 (p40:p35) plasmid with the Not I and Swa I (blunt end) restriction enzymes and ligated to the pCR-blunt psiCAT plasmid cut with Not I and Pml I (blunt end). In the resulting plasmid, the promoter region was replaced with the p21 promoter from the p21-hIL-12-ORT plasmid using the Not I and Sal I restriction enzymes. The chloramphenicol antibiotic resistance gene (CmR) was then removed from the p21-mIL-12-Xmark plasmid using the ORT technology, resulting in the p21-mIL-12-ORT plasmid. ( b ) Annotated plasmid map: p21 promoter–promoter region from the native human p21 (CDKN1A) gene, mIL-12 (p40:p35) murine IL-12 intronless open reading frame consisting of the IL-12b (p40, beta subunit) and IL-12a (p35, alpha subunit) genes, SV40 polyA-simian virus 40 late polyadenylation signal, ORI- E. coli origin of replication, LacP lactose operon promoter with the lacO operator. ( c , d ) Restriction analysis: ( c ) the plasmid was cut with different combinations of restriction enzymes and its identity was confirmed by positive matching of the pattern of bands on the electrophoresis gel to the expected ( d ) pattern obtained by means of a simulation experiment using the SnapGene software. For the uncut plasmid, the simulated band pattern differs from the actual pattern because simulation can only be done for a supercoiled monomer, while other forms (supercoiled dimer, open circular, linear, nicked) can also be seen on the electrophoretic gel. Electrophoresis details: 1% agarose (Sigma-Aldrich), run for 45 min at 100 V/cm, stained in 1× Sybr Gold (Thermo Fisher Scientific). LL (linear <t>DNA</t> ladder): GeneRuler™ 1 kb Plus <t>DNA</t> <t>Ladder</t> (Thermo Fisher Scientific), lane 1: HindIII + MunI (2338 bp, 1792 bp, 1338 bp, 409 bp, 79 bp, 19 bp), lane 2: HindIII (3130 bp, 2338 bp, 409 bp, 79 bp, 19 bp), lane 3: KpnI (3936 bp, 2039 bp), lane 4: NcoI + Alw44I (3633 bp, 2342 bp), lane 5: BamHI + XbaI (3150 bp, 1652 bp, 1173 bp), lane 6: KpnI + Alw44I (3936 bp, 1314 bp, 725 bp), lane 7: NotI + Alw44I (4787 bp, 1188 bp), lane 8: uncut (supercoiled 5975 bp), SC (supercoiled DNA ladder): Supercoiled DNA Ladder (New England BioLabs, Ipswich, MA, USA).
    Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Non-Clinical In Vitro Evaluation of Antibiotic Resistance Gene-Free Plasmids Encoding Human or Murine IL-12 Intended for First-in-Human Clinical Study"

    Article Title: Non-Clinical In Vitro Evaluation of Antibiotic Resistance Gene-Free Plasmids Encoding Human or Murine IL-12 Intended for First-in-Human Clinical Study

    Journal: Pharmaceutics

    doi: 10.3390/pharmaceutics13101739

    Construction and confirmation of the p21-mIL-12-ORT plasmid. ( a ) Cloning plan created using the SnapGene software: the expression cassette carrying the murine IL-12 sequence under the EF1/HTLV promoter was cut out of the pORF-mIL-12 (p40:p35) plasmid with the Not I and Swa I (blunt end) restriction enzymes and ligated to the pCR-blunt psiCAT plasmid cut with Not I and Pml I (blunt end). In the resulting plasmid, the promoter region was replaced with the p21 promoter from the p21-hIL-12-ORT plasmid using the Not I and Sal I restriction enzymes. The chloramphenicol antibiotic resistance gene (CmR) was then removed from the p21-mIL-12-Xmark plasmid using the ORT technology, resulting in the p21-mIL-12-ORT plasmid. ( b ) Annotated plasmid map: p21 promoter–promoter region from the native human p21 (CDKN1A) gene, mIL-12 (p40:p35) murine IL-12 intronless open reading frame consisting of the IL-12b (p40, beta subunit) and IL-12a (p35, alpha subunit) genes, SV40 polyA-simian virus 40 late polyadenylation signal, ORI- E. coli origin of replication, LacP lactose operon promoter with the lacO operator. ( c , d ) Restriction analysis: ( c ) the plasmid was cut with different combinations of restriction enzymes and its identity was confirmed by positive matching of the pattern of bands on the electrophoresis gel to the expected ( d ) pattern obtained by means of a simulation experiment using the SnapGene software. For the uncut plasmid, the simulated band pattern differs from the actual pattern because simulation can only be done for a supercoiled monomer, while other forms (supercoiled dimer, open circular, linear, nicked) can also be seen on the electrophoretic gel. Electrophoresis details: 1% agarose (Sigma-Aldrich), run for 45 min at 100 V/cm, stained in 1× Sybr Gold (Thermo Fisher Scientific). LL (linear DNA ladder): GeneRuler™ 1 kb Plus DNA Ladder (Thermo Fisher Scientific), lane 1: HindIII + MunI (2338 bp, 1792 bp, 1338 bp, 409 bp, 79 bp, 19 bp), lane 2: HindIII (3130 bp, 2338 bp, 409 bp, 79 bp, 19 bp), lane 3: KpnI (3936 bp, 2039 bp), lane 4: NcoI + Alw44I (3633 bp, 2342 bp), lane 5: BamHI + XbaI (3150 bp, 1652 bp, 1173 bp), lane 6: KpnI + Alw44I (3936 bp, 1314 bp, 725 bp), lane 7: NotI + Alw44I (4787 bp, 1188 bp), lane 8: uncut (supercoiled 5975 bp), SC (supercoiled DNA ladder): Supercoiled DNA Ladder (New England BioLabs, Ipswich, MA, USA).
    Figure Legend Snippet: Construction and confirmation of the p21-mIL-12-ORT plasmid. ( a ) Cloning plan created using the SnapGene software: the expression cassette carrying the murine IL-12 sequence under the EF1/HTLV promoter was cut out of the pORF-mIL-12 (p40:p35) plasmid with the Not I and Swa I (blunt end) restriction enzymes and ligated to the pCR-blunt psiCAT plasmid cut with Not I and Pml I (blunt end). In the resulting plasmid, the promoter region was replaced with the p21 promoter from the p21-hIL-12-ORT plasmid using the Not I and Sal I restriction enzymes. The chloramphenicol antibiotic resistance gene (CmR) was then removed from the p21-mIL-12-Xmark plasmid using the ORT technology, resulting in the p21-mIL-12-ORT plasmid. ( b ) Annotated plasmid map: p21 promoter–promoter region from the native human p21 (CDKN1A) gene, mIL-12 (p40:p35) murine IL-12 intronless open reading frame consisting of the IL-12b (p40, beta subunit) and IL-12a (p35, alpha subunit) genes, SV40 polyA-simian virus 40 late polyadenylation signal, ORI- E. coli origin of replication, LacP lactose operon promoter with the lacO operator. ( c , d ) Restriction analysis: ( c ) the plasmid was cut with different combinations of restriction enzymes and its identity was confirmed by positive matching of the pattern of bands on the electrophoresis gel to the expected ( d ) pattern obtained by means of a simulation experiment using the SnapGene software. For the uncut plasmid, the simulated band pattern differs from the actual pattern because simulation can only be done for a supercoiled monomer, while other forms (supercoiled dimer, open circular, linear, nicked) can also be seen on the electrophoretic gel. Electrophoresis details: 1% agarose (Sigma-Aldrich), run for 45 min at 100 V/cm, stained in 1× Sybr Gold (Thermo Fisher Scientific). LL (linear DNA ladder): GeneRuler™ 1 kb Plus DNA Ladder (Thermo Fisher Scientific), lane 1: HindIII + MunI (2338 bp, 1792 bp, 1338 bp, 409 bp, 79 bp, 19 bp), lane 2: HindIII (3130 bp, 2338 bp, 409 bp, 79 bp, 19 bp), lane 3: KpnI (3936 bp, 2039 bp), lane 4: NcoI + Alw44I (3633 bp, 2342 bp), lane 5: BamHI + XbaI (3150 bp, 1652 bp, 1173 bp), lane 6: KpnI + Alw44I (3936 bp, 1314 bp, 725 bp), lane 7: NotI + Alw44I (4787 bp, 1188 bp), lane 8: uncut (supercoiled 5975 bp), SC (supercoiled DNA ladder): Supercoiled DNA Ladder (New England BioLabs, Ipswich, MA, USA).

    Techniques Used: Plasmid Preparation, Clone Assay, Software, Expressing, Sequencing, Electrophoresis, Nucleic Acid Electrophoresis, Staining

    dna ladder  (New England Biolabs)


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    New England Biolabs dna ladder
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    New England Biolabs dna ladder
    P. abyssi RadA recombinase activity catalyzed displacement-loop (D-loop) formation. ( A ) Schematic representation for the D-loop formation assay. Labeled linear ssDNA (93 nt) was incubated first with RadA to form nucleoprotein filaments before adding the <t>purified</t> <t>supercoiled</t> plasmid pUC19 for further homology search. ( B ) D-loop formation assay with increased quantity of RadA. An amount of 25 nM of labeled ssDNA (93 nt) was incubated with RadA for 10 min at 65 °C. Then, 25 nM of purified supercoiled pUC19 was added and incubated for another 10 min. <t>DNA</t> products were separated on a 1.2% native agarose gel and visualized by fluorescence. ( C ) Histogram representation of the D-loop formation assays for a range of RadA as observed in ( B ). RadA-dependent D-loop (%), densitometry measurement of formed D-loop as a percentage of total lane densitometry after data normalization and the D-loop background from lane 3 was subtracted. Experiments were performed in triplicate.
    Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Role of RadA and DNA Polymerases in Recombination-Associated DNA Synthesis in Hyperthermophilic Archaea"

    Article Title: Role of RadA and DNA Polymerases in Recombination-Associated DNA Synthesis in Hyperthermophilic Archaea

    Journal: Biomolecules

    doi: 10.3390/biom10071045

    P. abyssi RadA recombinase activity catalyzed displacement-loop (D-loop) formation. ( A ) Schematic representation for the D-loop formation assay. Labeled linear ssDNA (93 nt) was incubated first with RadA to form nucleoprotein filaments before adding the purified supercoiled plasmid pUC19 for further homology search. ( B ) D-loop formation assay with increased quantity of RadA. An amount of 25 nM of labeled ssDNA (93 nt) was incubated with RadA for 10 min at 65 °C. Then, 25 nM of purified supercoiled pUC19 was added and incubated for another 10 min. DNA products were separated on a 1.2% native agarose gel and visualized by fluorescence. ( C ) Histogram representation of the D-loop formation assays for a range of RadA as observed in ( B ). RadA-dependent D-loop (%), densitometry measurement of formed D-loop as a percentage of total lane densitometry after data normalization and the D-loop background from lane 3 was subtracted. Experiments were performed in triplicate.
    Figure Legend Snippet: P. abyssi RadA recombinase activity catalyzed displacement-loop (D-loop) formation. ( A ) Schematic representation for the D-loop formation assay. Labeled linear ssDNA (93 nt) was incubated first with RadA to form nucleoprotein filaments before adding the purified supercoiled plasmid pUC19 for further homology search. ( B ) D-loop formation assay with increased quantity of RadA. An amount of 25 nM of labeled ssDNA (93 nt) was incubated with RadA for 10 min at 65 °C. Then, 25 nM of purified supercoiled pUC19 was added and incubated for another 10 min. DNA products were separated on a 1.2% native agarose gel and visualized by fluorescence. ( C ) Histogram representation of the D-loop formation assays for a range of RadA as observed in ( B ). RadA-dependent D-loop (%), densitometry measurement of formed D-loop as a percentage of total lane densitometry after data normalization and the D-loop background from lane 3 was subtracted. Experiments were performed in triplicate.

    Techniques Used: Activity Assay, Tube Formation Assay, Labeling, Incubation, Purification, Plasmid Preparation, Agarose Gel Electrophoresis, Fluorescence

    Addition of PCNA stimulates DNA extension by DNA polymerases on recombination intermediates. ( A ) Schematic representation for DNA extension by family-B polymerase (PolB) or family-D polymerase (PolD) following the D-loop formation by RadA described in A. ( B ) Recombination-associated DNA synthesis assay. An amount of 25 nM of labeled ssDNA was first incubated with 1.6 µM RadA for 10 min at 65 °C. Then, 25 nM of purified supercoiled pUC19 was added and incubated for another 10 min. D-loop provided by RadA strand exchange activity was extended by 675 nM of PolB or PolD for 1 hr at 65 °C. DNA products were separated on a 1.2% native agarose gel. Same DNA products from ( B ) were separated as well in 5% denaturing acrylamide gel ( C ) or 1% denaturing alkaline agarose gel ( D ). When indicated, 675 nM of PCNA was added together with DNA polymerases. DNA products were revealed by fluorescence for HiLyte TM 647 labeled DNA. The denaturing alkaline agarose gel ( D ) was also stained by SYBR Gold to detect the DNA ladder (lane 1) and pUC19 plasmid (lane 2). For all the experiments, controls were treated as the assays (volume and incubation time), when a protein was absent it was replaced by the corresponding buffer. The two bands at the top of the gel in lanes 3 to 12 are non-specific products corresponding to incomplete denaturation of pUC19 plasmid with residual labelled ssDNA fixed on melted regions.
    Figure Legend Snippet: Addition of PCNA stimulates DNA extension by DNA polymerases on recombination intermediates. ( A ) Schematic representation for DNA extension by family-B polymerase (PolB) or family-D polymerase (PolD) following the D-loop formation by RadA described in A. ( B ) Recombination-associated DNA synthesis assay. An amount of 25 nM of labeled ssDNA was first incubated with 1.6 µM RadA for 10 min at 65 °C. Then, 25 nM of purified supercoiled pUC19 was added and incubated for another 10 min. D-loop provided by RadA strand exchange activity was extended by 675 nM of PolB or PolD for 1 hr at 65 °C. DNA products were separated on a 1.2% native agarose gel. Same DNA products from ( B ) were separated as well in 5% denaturing acrylamide gel ( C ) or 1% denaturing alkaline agarose gel ( D ). When indicated, 675 nM of PCNA was added together with DNA polymerases. DNA products were revealed by fluorescence for HiLyte TM 647 labeled DNA. The denaturing alkaline agarose gel ( D ) was also stained by SYBR Gold to detect the DNA ladder (lane 1) and pUC19 plasmid (lane 2). For all the experiments, controls were treated as the assays (volume and incubation time), when a protein was absent it was replaced by the corresponding buffer. The two bands at the top of the gel in lanes 3 to 12 are non-specific products corresponding to incomplete denaturation of pUC19 plasmid with residual labelled ssDNA fixed on melted regions.

    Techniques Used: DNA Synthesis, Labeling, Incubation, Purification, Activity Assay, Agarose Gel Electrophoresis, Acrylamide Gel Assay, Fluorescence, Staining, Plasmid Preparation

    dna ladder  (New England Biolabs)


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    Structured Review

    New England Biolabs dna ladder
    Agarose gel electrophoresis of <t>DNA</t> extracts from BCC9546. ( a ) Genomic DNA running on 1% agarose gel: M, 250 ng DNA size marker (GeneRuler <t>DNA</t> <t>Ladder</t> Mix, Thermo Scientific); 1–2, 250 ng BCC9546 genomic DNA extract. ( b ) Plasmid DNA running on 0.5% agarose gel: Ms, 250 ng supercoiled DNA Ladder (New England BioLabs); p1, 50 ng BCC9546 plasmid DNA extract; p2, 120 ng BCC9546 plasmid DNA extract. The gel images were cropped for concise visualization. See Supplementary Fig. for the uncropped gel images.
    Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Safety Assessment of a Nham Starter Culture Lactobacillus plantarum BCC9546 via Whole-genome Analysis"

    Article Title: Safety Assessment of a Nham Starter Culture Lactobacillus plantarum BCC9546 via Whole-genome Analysis

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-66857-2

    Agarose gel electrophoresis of DNA extracts from BCC9546. ( a ) Genomic DNA running on 1% agarose gel: M, 250 ng DNA size marker (GeneRuler DNA Ladder Mix, Thermo Scientific); 1–2, 250 ng BCC9546 genomic DNA extract. ( b ) Plasmid DNA running on 0.5% agarose gel: Ms, 250 ng supercoiled DNA Ladder (New England BioLabs); p1, 50 ng BCC9546 plasmid DNA extract; p2, 120 ng BCC9546 plasmid DNA extract. The gel images were cropped for concise visualization. See Supplementary Fig. for the uncropped gel images.
    Figure Legend Snippet: Agarose gel electrophoresis of DNA extracts from BCC9546. ( a ) Genomic DNA running on 1% agarose gel: M, 250 ng DNA size marker (GeneRuler DNA Ladder Mix, Thermo Scientific); 1–2, 250 ng BCC9546 genomic DNA extract. ( b ) Plasmid DNA running on 0.5% agarose gel: Ms, 250 ng supercoiled DNA Ladder (New England BioLabs); p1, 50 ng BCC9546 plasmid DNA extract; p2, 120 ng BCC9546 plasmid DNA extract. The gel images were cropped for concise visualization. See Supplementary Fig. for the uncropped gel images.

    Techniques Used: Agarose Gel Electrophoresis, Marker, Plasmid Preparation

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    New England Biolabs dna ladder
    Electrophoretic profile of plasmids extracted from isolated strains. a Electrophoresis was performed on 0.8% agarose gels at 75 V for 16 h. As a comparison marker, the Supercoiled <t>DNA</t> <t>Ladder</t> was used. b Estimation of the size of the plasmid, comparing the isoform profile with the isoform profile contained in the supercoiled DNA Ladder
    Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bac tracker supercoiled dna ladder
    Electrophoretic profile of plasmids extracted from isolated strains. a Electrophoresis was performed on 0.8% agarose gels at 75 V for 16 h. As a comparison marker, the Supercoiled <t>DNA</t> <t>Ladder</t> was used. b Estimation of the size of the plasmid, comparing the isoform profile with the isoform profile contained in the supercoiled DNA Ladder
    Bac Tracker Supercoiled Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs 2 log dna ladder
    Electrophoretic profile of plasmids extracted from isolated strains. a Electrophoresis was performed on 0.8% agarose gels at 75 V for 16 h. As a comparison marker, the Supercoiled <t>DNA</t> <t>Ladder</t> was used. b Estimation of the size of the plasmid, comparing the isoform profile with the isoform profile contained in the supercoiled DNA Ladder
    2 Log Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Electrophoretic profile of plasmids extracted from isolated strains. a Electrophoresis was performed on 0.8% agarose gels at 75 V for 16 h. As a comparison marker, the Supercoiled DNA Ladder was used. b Estimation of the size of the plasmid, comparing the isoform profile with the isoform profile contained in the supercoiled DNA Ladder

    Journal: Current Microbiology

    Article Title: Regulation Transcriptional of Antibiotic Resistance Genes (ARGs) in Bacteria Isolated from WWTP

    doi: 10.1007/s00284-023-03449-z

    Figure Lengend Snippet: Electrophoretic profile of plasmids extracted from isolated strains. a Electrophoresis was performed on 0.8% agarose gels at 75 V for 16 h. As a comparison marker, the Supercoiled DNA Ladder was used. b Estimation of the size of the plasmid, comparing the isoform profile with the isoform profile contained in the supercoiled DNA Ladder

    Article Snippet: The determination of the molecular weight of the plasmids was based on the comparison of the isoforms of the native plasmids with the electrophoretic profile of the supercoiled DNA Ladder (#N0472, NEW ENGLANDS BioLabs).

    Techniques: Isolation, Electrophoresis, Marker, Plasmid Preparation