quick load 1 kb dna ladder  (New England Biolabs)


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    Name:
    Quick Load 1 kb DNA Ladder
    Description:
    Quick Load 1 kb DNA Ladder 375 gel lanes
    Catalog Number:
    N0468L
    Price:
    153
    Category:
    DNA Ladders
    Size:
    375 gel lanes
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    New England Biolabs quick load 1 kb dna ladder
    Quick Load 1 kb DNA Ladder
    Quick Load 1 kb DNA Ladder 375 gel lanes
    https://www.bioz.com/result/quick load 1 kb dna ladder/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quick load 1 kb dna ladder - by Bioz Stars, 2021-05
    96/100 stars

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    Staining:

    Article Title: Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
    Article Snippet: The primers were purchased from IDT (Coralville, IA). .. Reagents and devices used include the following: acetyl-CoA, acetoacetyl-CoA, ( S )-3-hydroxybutyryl-CoA, crotonyl-CoA, butyryl-CoA, butyraldehyde, acetaldehyde, NADH, 1 mg/ml bovine serum albumin protein standard, and protein molecular mass standards (Sigma-Aldrich, St. Louis, MO); buffer components, medium components, and GelCode blue stain reagent (Thermo Fisher Scientific, Pittsburgh, PA); NuPAGE 4 to 12% Bis-Tris protein gels and BenchMark protein standards (10 to 220 kDa) (Life Technologies, Grand Island, NY); Bio-Rad protein assay dye reagent (Hercules, CA); QIAquick PCR purification and QIAprep spin miniprep kits (Qiagen, Inc., Valencia, CA); restriction enzymes, Gibson assembly master mix, and Quickload DNA ladder (1 kb) (New England BioLabs, Ipswich, MA); Amicon Ultra 10K centrifugal filter units (EMD Millipore, Billerica, MA); HisTrap HP, HiLoad Q-Sepharose XK 16/10, HiLoad Superdex 200 pg 26/600, and Superdex 200 10/300 GL fast-performance liquid chromatography (FPLC) columns (GE Healthcare); ZB-WAXplus capillary gas chromatography (GC) column (30 m long, 0.53-mm inner diameter [ID], 1-μm film thickness) (Phenomenex, Torrance, CA). .. TTE0544 ( crt ), TTE0548 ( hbd ), TTE0549 ( thl ), Teth514_1935 (X514- bdh ), and Teth514_1942 (X514- bad ) were amplified by PCR from genomic DNA (gDNA) ( Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 or Thermoanaerobacter sp. strain X514 ATCC BAA-938) using primers shown in Table S1 in the supplemental material and ligated into pET-46 Ek/LIC.

    Polymerase Chain Reaction:

    Article Title: Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
    Article Snippet: The primers were purchased from IDT (Coralville, IA). .. Reagents and devices used include the following: acetyl-CoA, acetoacetyl-CoA, ( S )-3-hydroxybutyryl-CoA, crotonyl-CoA, butyryl-CoA, butyraldehyde, acetaldehyde, NADH, 1 mg/ml bovine serum albumin protein standard, and protein molecular mass standards (Sigma-Aldrich, St. Louis, MO); buffer components, medium components, and GelCode blue stain reagent (Thermo Fisher Scientific, Pittsburgh, PA); NuPAGE 4 to 12% Bis-Tris protein gels and BenchMark protein standards (10 to 220 kDa) (Life Technologies, Grand Island, NY); Bio-Rad protein assay dye reagent (Hercules, CA); QIAquick PCR purification and QIAprep spin miniprep kits (Qiagen, Inc., Valencia, CA); restriction enzymes, Gibson assembly master mix, and Quickload DNA ladder (1 kb) (New England BioLabs, Ipswich, MA); Amicon Ultra 10K centrifugal filter units (EMD Millipore, Billerica, MA); HisTrap HP, HiLoad Q-Sepharose XK 16/10, HiLoad Superdex 200 pg 26/600, and Superdex 200 10/300 GL fast-performance liquid chromatography (FPLC) columns (GE Healthcare); ZB-WAXplus capillary gas chromatography (GC) column (30 m long, 0.53-mm inner diameter [ID], 1-μm film thickness) (Phenomenex, Torrance, CA). .. TTE0544 ( crt ), TTE0548 ( hbd ), TTE0549 ( thl ), Teth514_1935 (X514- bdh ), and Teth514_1942 (X514- bad ) were amplified by PCR from genomic DNA (gDNA) ( Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 or Thermoanaerobacter sp. strain X514 ATCC BAA-938) using primers shown in Table S1 in the supplemental material and ligated into pET-46 Ek/LIC.

    Article Title: Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis
    Article Snippet: The PCR amplification started with 95°C for initial denaturation, followed by 30 cycles of denaturation at 95°C for 30 s, annealing at 48°C for 30 s, and extension at 72°C for 1 min. .. The final extension was done at 72°C for 10 min. PCR products were visualized on 1% agarose gel (1× TAE-buffer: 40 mM Tris base, 1 mM EDTA, pH 8 adjusted with acetic acid; 0.1 μg/ml ethidium bromide) after carrying out gel electrophoresis of each PCR amplification product and 6 μl Quick Load 1 kb DNA Ladder (New England Biolabs, Frankfurt/Main, Germany; N0468 S) with 1× TAE buffer at 100 V for 1 h. Distilled water was used as negative control. ..

    Purification:

    Article Title: Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
    Article Snippet: The primers were purchased from IDT (Coralville, IA). .. Reagents and devices used include the following: acetyl-CoA, acetoacetyl-CoA, ( S )-3-hydroxybutyryl-CoA, crotonyl-CoA, butyryl-CoA, butyraldehyde, acetaldehyde, NADH, 1 mg/ml bovine serum albumin protein standard, and protein molecular mass standards (Sigma-Aldrich, St. Louis, MO); buffer components, medium components, and GelCode blue stain reagent (Thermo Fisher Scientific, Pittsburgh, PA); NuPAGE 4 to 12% Bis-Tris protein gels and BenchMark protein standards (10 to 220 kDa) (Life Technologies, Grand Island, NY); Bio-Rad protein assay dye reagent (Hercules, CA); QIAquick PCR purification and QIAprep spin miniprep kits (Qiagen, Inc., Valencia, CA); restriction enzymes, Gibson assembly master mix, and Quickload DNA ladder (1 kb) (New England BioLabs, Ipswich, MA); Amicon Ultra 10K centrifugal filter units (EMD Millipore, Billerica, MA); HisTrap HP, HiLoad Q-Sepharose XK 16/10, HiLoad Superdex 200 pg 26/600, and Superdex 200 10/300 GL fast-performance liquid chromatography (FPLC) columns (GE Healthcare); ZB-WAXplus capillary gas chromatography (GC) column (30 m long, 0.53-mm inner diameter [ID], 1-μm film thickness) (Phenomenex, Torrance, CA). .. TTE0544 ( crt ), TTE0548 ( hbd ), TTE0549 ( thl ), Teth514_1935 (X514- bdh ), and Teth514_1942 (X514- bad ) were amplified by PCR from genomic DNA (gDNA) ( Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 or Thermoanaerobacter sp. strain X514 ATCC BAA-938) using primers shown in Table S1 in the supplemental material and ligated into pET-46 Ek/LIC.

    Liquid Chromatography:

    Article Title: Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
    Article Snippet: The primers were purchased from IDT (Coralville, IA). .. Reagents and devices used include the following: acetyl-CoA, acetoacetyl-CoA, ( S )-3-hydroxybutyryl-CoA, crotonyl-CoA, butyryl-CoA, butyraldehyde, acetaldehyde, NADH, 1 mg/ml bovine serum albumin protein standard, and protein molecular mass standards (Sigma-Aldrich, St. Louis, MO); buffer components, medium components, and GelCode blue stain reagent (Thermo Fisher Scientific, Pittsburgh, PA); NuPAGE 4 to 12% Bis-Tris protein gels and BenchMark protein standards (10 to 220 kDa) (Life Technologies, Grand Island, NY); Bio-Rad protein assay dye reagent (Hercules, CA); QIAquick PCR purification and QIAprep spin miniprep kits (Qiagen, Inc., Valencia, CA); restriction enzymes, Gibson assembly master mix, and Quickload DNA ladder (1 kb) (New England BioLabs, Ipswich, MA); Amicon Ultra 10K centrifugal filter units (EMD Millipore, Billerica, MA); HisTrap HP, HiLoad Q-Sepharose XK 16/10, HiLoad Superdex 200 pg 26/600, and Superdex 200 10/300 GL fast-performance liquid chromatography (FPLC) columns (GE Healthcare); ZB-WAXplus capillary gas chromatography (GC) column (30 m long, 0.53-mm inner diameter [ID], 1-μm film thickness) (Phenomenex, Torrance, CA). .. TTE0544 ( crt ), TTE0548 ( hbd ), TTE0549 ( thl ), Teth514_1935 (X514- bdh ), and Teth514_1942 (X514- bad ) were amplified by PCR from genomic DNA (gDNA) ( Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 or Thermoanaerobacter sp. strain X514 ATCC BAA-938) using primers shown in Table S1 in the supplemental material and ligated into pET-46 Ek/LIC.

    Fast Protein Liquid Chromatography:

    Article Title: Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
    Article Snippet: The primers were purchased from IDT (Coralville, IA). .. Reagents and devices used include the following: acetyl-CoA, acetoacetyl-CoA, ( S )-3-hydroxybutyryl-CoA, crotonyl-CoA, butyryl-CoA, butyraldehyde, acetaldehyde, NADH, 1 mg/ml bovine serum albumin protein standard, and protein molecular mass standards (Sigma-Aldrich, St. Louis, MO); buffer components, medium components, and GelCode blue stain reagent (Thermo Fisher Scientific, Pittsburgh, PA); NuPAGE 4 to 12% Bis-Tris protein gels and BenchMark protein standards (10 to 220 kDa) (Life Technologies, Grand Island, NY); Bio-Rad protein assay dye reagent (Hercules, CA); QIAquick PCR purification and QIAprep spin miniprep kits (Qiagen, Inc., Valencia, CA); restriction enzymes, Gibson assembly master mix, and Quickload DNA ladder (1 kb) (New England BioLabs, Ipswich, MA); Amicon Ultra 10K centrifugal filter units (EMD Millipore, Billerica, MA); HisTrap HP, HiLoad Q-Sepharose XK 16/10, HiLoad Superdex 200 pg 26/600, and Superdex 200 10/300 GL fast-performance liquid chromatography (FPLC) columns (GE Healthcare); ZB-WAXplus capillary gas chromatography (GC) column (30 m long, 0.53-mm inner diameter [ID], 1-μm film thickness) (Phenomenex, Torrance, CA). .. TTE0544 ( crt ), TTE0548 ( hbd ), TTE0549 ( thl ), Teth514_1935 (X514- bdh ), and Teth514_1942 (X514- bad ) were amplified by PCR from genomic DNA (gDNA) ( Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 or Thermoanaerobacter sp. strain X514 ATCC BAA-938) using primers shown in Table S1 in the supplemental material and ligated into pET-46 Ek/LIC.

    Gas Chromatography:

    Article Title: Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
    Article Snippet: The primers were purchased from IDT (Coralville, IA). .. Reagents and devices used include the following: acetyl-CoA, acetoacetyl-CoA, ( S )-3-hydroxybutyryl-CoA, crotonyl-CoA, butyryl-CoA, butyraldehyde, acetaldehyde, NADH, 1 mg/ml bovine serum albumin protein standard, and protein molecular mass standards (Sigma-Aldrich, St. Louis, MO); buffer components, medium components, and GelCode blue stain reagent (Thermo Fisher Scientific, Pittsburgh, PA); NuPAGE 4 to 12% Bis-Tris protein gels and BenchMark protein standards (10 to 220 kDa) (Life Technologies, Grand Island, NY); Bio-Rad protein assay dye reagent (Hercules, CA); QIAquick PCR purification and QIAprep spin miniprep kits (Qiagen, Inc., Valencia, CA); restriction enzymes, Gibson assembly master mix, and Quickload DNA ladder (1 kb) (New England BioLabs, Ipswich, MA); Amicon Ultra 10K centrifugal filter units (EMD Millipore, Billerica, MA); HisTrap HP, HiLoad Q-Sepharose XK 16/10, HiLoad Superdex 200 pg 26/600, and Superdex 200 10/300 GL fast-performance liquid chromatography (FPLC) columns (GE Healthcare); ZB-WAXplus capillary gas chromatography (GC) column (30 m long, 0.53-mm inner diameter [ID], 1-μm film thickness) (Phenomenex, Torrance, CA). .. TTE0544 ( crt ), TTE0548 ( hbd ), TTE0549 ( thl ), Teth514_1935 (X514- bdh ), and Teth514_1942 (X514- bad ) were amplified by PCR from genomic DNA (gDNA) ( Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 or Thermoanaerobacter sp. strain X514 ATCC BAA-938) using primers shown in Table S1 in the supplemental material and ligated into pET-46 Ek/LIC.

    Plasmid Preparation:

    Article Title: Purifying Properly Folded Cysteine-rich, Zinc Finger Containing Recombinant Proteins for Structural Drug Targeting Studies: the CH1 Domain of p300 as a Case Example
    Article Snippet: Ice LB Miller broth powdered (Fisher Scientific, catalog number: BP1426-2) Ampicillin sodium salt powdered (Sigma-Aldrich, catalog number: A9518) Note: Prepare 100 mM stock (1,000×) in deionized water. .. Plasmid DNA Extraction Miniprep Kit (GeneJET) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: K0502) Bam HI restriction enzyme FastDigest (800 µl, 1 reaction/1 µl) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: FD0054); store at −20 °C Restriction enzyme buffer FastDigest (10×; 5× 1 ml) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: B64); store at −20 °C Eco RI restriction enzyme FastDigest (800 µl, 1 reaction/1 µl) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: FD0274); store at −20 °C Quick-Load 1 kb DNA ladder (New England Biolabs) 5× nucleic acid loading buffer (10 ml, premixed) (Bio-Rad Laboratories, catalog number: 1610767) Deionized water Acrylamide electrophoresis gels (Criterion TGX Precast Gels; 18 well comb, 30 µl loading volume per well, 1.0 mm thickness, 4–20% gel percentage) (Bio-Rad Laboratories, catalog number: 5671094) Agar powdered (Fisher Scientific, catalog number: BP24662) 1× TAE running buffer Ethidium bromide (10 mg/ml) (Thermo Fisher Scientific, Invitrogen™, catalog number: 15585011) Glycerol 100% (EMD Millipore, catalog number: GX0185) Isopropyl β-D-1-thiogalactopyranoside (IPTG) powdered (Gold Bio, catalog number: I2481C100) Note: Prepare 10 ml of 1 M (10,000×) stock in deionized water and store at −20 °C . .. Zinc chloride (ZnCl2 ), 99.999% trace metals basis powdered (Sigma-Aldrich, catalog number: 229997) Note: Prepare as 100 mM stock in deionized water .

    DNA Extraction:

    Article Title: Purifying Properly Folded Cysteine-rich, Zinc Finger Containing Recombinant Proteins for Structural Drug Targeting Studies: the CH1 Domain of p300 as a Case Example
    Article Snippet: Ice LB Miller broth powdered (Fisher Scientific, catalog number: BP1426-2) Ampicillin sodium salt powdered (Sigma-Aldrich, catalog number: A9518) Note: Prepare 100 mM stock (1,000×) in deionized water. .. Plasmid DNA Extraction Miniprep Kit (GeneJET) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: K0502) Bam HI restriction enzyme FastDigest (800 µl, 1 reaction/1 µl) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: FD0054); store at −20 °C Restriction enzyme buffer FastDigest (10×; 5× 1 ml) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: B64); store at −20 °C Eco RI restriction enzyme FastDigest (800 µl, 1 reaction/1 µl) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: FD0274); store at −20 °C Quick-Load 1 kb DNA ladder (New England Biolabs) 5× nucleic acid loading buffer (10 ml, premixed) (Bio-Rad Laboratories, catalog number: 1610767) Deionized water Acrylamide electrophoresis gels (Criterion TGX Precast Gels; 18 well comb, 30 µl loading volume per well, 1.0 mm thickness, 4–20% gel percentage) (Bio-Rad Laboratories, catalog number: 5671094) Agar powdered (Fisher Scientific, catalog number: BP24662) 1× TAE running buffer Ethidium bromide (10 mg/ml) (Thermo Fisher Scientific, Invitrogen™, catalog number: 15585011) Glycerol 100% (EMD Millipore, catalog number: GX0185) Isopropyl β-D-1-thiogalactopyranoside (IPTG) powdered (Gold Bio, catalog number: I2481C100) Note: Prepare 10 ml of 1 M (10,000×) stock in deionized water and store at −20 °C . .. Zinc chloride (ZnCl2 ), 99.999% trace metals basis powdered (Sigma-Aldrich, catalog number: 229997) Note: Prepare as 100 mM stock in deionized water .

    Electrophoresis:

    Article Title: Purifying Properly Folded Cysteine-rich, Zinc Finger Containing Recombinant Proteins for Structural Drug Targeting Studies: the CH1 Domain of p300 as a Case Example
    Article Snippet: Ice LB Miller broth powdered (Fisher Scientific, catalog number: BP1426-2) Ampicillin sodium salt powdered (Sigma-Aldrich, catalog number: A9518) Note: Prepare 100 mM stock (1,000×) in deionized water. .. Plasmid DNA Extraction Miniprep Kit (GeneJET) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: K0502) Bam HI restriction enzyme FastDigest (800 µl, 1 reaction/1 µl) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: FD0054); store at −20 °C Restriction enzyme buffer FastDigest (10×; 5× 1 ml) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: B64); store at −20 °C Eco RI restriction enzyme FastDigest (800 µl, 1 reaction/1 µl) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: FD0274); store at −20 °C Quick-Load 1 kb DNA ladder (New England Biolabs) 5× nucleic acid loading buffer (10 ml, premixed) (Bio-Rad Laboratories, catalog number: 1610767) Deionized water Acrylamide electrophoresis gels (Criterion TGX Precast Gels; 18 well comb, 30 µl loading volume per well, 1.0 mm thickness, 4–20% gel percentage) (Bio-Rad Laboratories, catalog number: 5671094) Agar powdered (Fisher Scientific, catalog number: BP24662) 1× TAE running buffer Ethidium bromide (10 mg/ml) (Thermo Fisher Scientific, Invitrogen™, catalog number: 15585011) Glycerol 100% (EMD Millipore, catalog number: GX0185) Isopropyl β-D-1-thiogalactopyranoside (IPTG) powdered (Gold Bio, catalog number: I2481C100) Note: Prepare 10 ml of 1 M (10,000×) stock in deionized water and store at −20 °C . .. Zinc chloride (ZnCl2 ), 99.999% trace metals basis powdered (Sigma-Aldrich, catalog number: 229997) Note: Prepare as 100 mM stock in deionized water .

    Article Title: Mapping of histone modifications in episomal HBV cccDNA uncovers an unusual chromatin organization amenable to epigenetic manipulation
    Article Snippet: .. For Southern blot analysis, 0.5 μL of Quick-Load 1-kb DNA ladder (New England Biolabs) and 15 μg ( ) or 25 μg ( ) of HIRT-extracted DNA were loaded per lane and separated by electrophoresis in a 1.2% (wt/vol) agarose gel in 1× Tris-Acetate-EDTA buffer. .. After electrophoresis the DNA was depurinated, denatured, and neutralized exactly as described by Cai et al. ( ) and transferred onto Hybond XL membrane (Amersham) by using the TurboBlotter system (GE Healthcare). (-) strand HBV DNA was detected with a DIG-labeled (+) strand HBV RNA probe transcribed from a ScaI-linearized pGEM3Z-HBV plasmid with DIG Northern Starter Kit (Roche) according to manufacturer’s instructions.

    Marker:

    Article Title: D-Serine Metabolism and Its Importance in Development of Dictyostelium discoideum
    Article Snippet: Supplementary Material The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2018.00784/full#supplementary-material FIGURE S1 Construction of dsd -null mutant. (A) The blasticidin S resistance gene (bsr ) was introduced into the Wild-type (WT) strain of Dictyostelium discoideum by homologous recombination. (B) Agarose gel electrophoresis of PCR-amplified DNA with DsdKOckf (a) and DsdKOckr (b) primers from the parental strain (WT) and the dsd -null mutants. .. The sizes of marker DNA fragments (Quick-Load 1 kB DNA ladder; New England BioLabs) are shown on the left. ..

    Southern Blot:

    Article Title: Mapping of histone modifications in episomal HBV cccDNA uncovers an unusual chromatin organization amenable to epigenetic manipulation
    Article Snippet: .. For Southern blot analysis, 0.5 μL of Quick-Load 1-kb DNA ladder (New England Biolabs) and 15 μg ( ) or 25 μg ( ) of HIRT-extracted DNA were loaded per lane and separated by electrophoresis in a 1.2% (wt/vol) agarose gel in 1× Tris-Acetate-EDTA buffer. .. After electrophoresis the DNA was depurinated, denatured, and neutralized exactly as described by Cai et al. ( ) and transferred onto Hybond XL membrane (Amersham) by using the TurboBlotter system (GE Healthcare). (-) strand HBV DNA was detected with a DIG-labeled (+) strand HBV RNA probe transcribed from a ScaI-linearized pGEM3Z-HBV plasmid with DIG Northern Starter Kit (Roche) according to manufacturer’s instructions.

    Agarose Gel Electrophoresis:

    Article Title: Mapping of histone modifications in episomal HBV cccDNA uncovers an unusual chromatin organization amenable to epigenetic manipulation
    Article Snippet: .. For Southern blot analysis, 0.5 μL of Quick-Load 1-kb DNA ladder (New England Biolabs) and 15 μg ( ) or 25 μg ( ) of HIRT-extracted DNA were loaded per lane and separated by electrophoresis in a 1.2% (wt/vol) agarose gel in 1× Tris-Acetate-EDTA buffer. .. After electrophoresis the DNA was depurinated, denatured, and neutralized exactly as described by Cai et al. ( ) and transferred onto Hybond XL membrane (Amersham) by using the TurboBlotter system (GE Healthcare). (-) strand HBV DNA was detected with a DIG-labeled (+) strand HBV RNA probe transcribed from a ScaI-linearized pGEM3Z-HBV plasmid with DIG Northern Starter Kit (Roche) according to manufacturer’s instructions.

    Article Title: Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis
    Article Snippet: The PCR amplification started with 95°C for initial denaturation, followed by 30 cycles of denaturation at 95°C for 30 s, annealing at 48°C for 30 s, and extension at 72°C for 1 min. .. The final extension was done at 72°C for 10 min. PCR products were visualized on 1% agarose gel (1× TAE-buffer: 40 mM Tris base, 1 mM EDTA, pH 8 adjusted with acetic acid; 0.1 μg/ml ethidium bromide) after carrying out gel electrophoresis of each PCR amplification product and 6 μl Quick Load 1 kb DNA Ladder (New England Biolabs, Frankfurt/Main, Germany; N0468 S) with 1× TAE buffer at 100 V for 1 h. Distilled water was used as negative control. ..

    Article Title: Molecular and phenotypic characterization of Als1 and Als2 mutations conferring tolerance to acetolactate synthase herbicides in soybean
    Article Snippet: .. Expression of Als1 and Als2 mutations Results of the cDNA cloning of the Als1 and Als2 mutations are shown in the 1% Tris-borate-EDTA (TBE) agarose gel in Fig. [30 min at 100 V, 1 kb ladder (NEB Cat. No. N0468S)]. .. The amplified cDNA fragments appear to match the expected fragment sizes of 2066 bp and 1942 bp for Als1 (see Fig. , lanes marked ‘a’ and ‘b’ respectively) and 2083 bp and 1957 bp for Als2 (see Fig. , lanes marked ‘c’ and ‘d’ respectively).

    Nucleic Acid Electrophoresis:

    Article Title: Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis
    Article Snippet: The PCR amplification started with 95°C for initial denaturation, followed by 30 cycles of denaturation at 95°C for 30 s, annealing at 48°C for 30 s, and extension at 72°C for 1 min. .. The final extension was done at 72°C for 10 min. PCR products were visualized on 1% agarose gel (1× TAE-buffer: 40 mM Tris base, 1 mM EDTA, pH 8 adjusted with acetic acid; 0.1 μg/ml ethidium bromide) after carrying out gel electrophoresis of each PCR amplification product and 6 μl Quick Load 1 kb DNA Ladder (New England Biolabs, Frankfurt/Main, Germany; N0468 S) with 1× TAE buffer at 100 V for 1 h. Distilled water was used as negative control. ..

    Amplification:

    Article Title: Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis
    Article Snippet: The PCR amplification started with 95°C for initial denaturation, followed by 30 cycles of denaturation at 95°C for 30 s, annealing at 48°C for 30 s, and extension at 72°C for 1 min. .. The final extension was done at 72°C for 10 min. PCR products were visualized on 1% agarose gel (1× TAE-buffer: 40 mM Tris base, 1 mM EDTA, pH 8 adjusted with acetic acid; 0.1 μg/ml ethidium bromide) after carrying out gel electrophoresis of each PCR amplification product and 6 μl Quick Load 1 kb DNA Ladder (New England Biolabs, Frankfurt/Main, Germany; N0468 S) with 1× TAE buffer at 100 V for 1 h. Distilled water was used as negative control. ..

    Negative Control:

    Article Title: Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis
    Article Snippet: The PCR amplification started with 95°C for initial denaturation, followed by 30 cycles of denaturation at 95°C for 30 s, annealing at 48°C for 30 s, and extension at 72°C for 1 min. .. The final extension was done at 72°C for 10 min. PCR products were visualized on 1% agarose gel (1× TAE-buffer: 40 mM Tris base, 1 mM EDTA, pH 8 adjusted with acetic acid; 0.1 μg/ml ethidium bromide) after carrying out gel electrophoresis of each PCR amplification product and 6 μl Quick Load 1 kb DNA Ladder (New England Biolabs, Frankfurt/Main, Germany; N0468 S) with 1× TAE buffer at 100 V for 1 h. Distilled water was used as negative control. ..

    Expressing:

    Article Title: Molecular and phenotypic characterization of Als1 and Als2 mutations conferring tolerance to acetolactate synthase herbicides in soybean
    Article Snippet: .. Expression of Als1 and Als2 mutations Results of the cDNA cloning of the Als1 and Als2 mutations are shown in the 1% Tris-borate-EDTA (TBE) agarose gel in Fig. [30 min at 100 V, 1 kb ladder (NEB Cat. No. N0468S)]. .. The amplified cDNA fragments appear to match the expected fragment sizes of 2066 bp and 1942 bp for Als1 (see Fig. , lanes marked ‘a’ and ‘b’ respectively) and 2083 bp and 1957 bp for Als2 (see Fig. , lanes marked ‘c’ and ‘d’ respectively).

    Clone Assay:

    Article Title: Molecular and phenotypic characterization of Als1 and Als2 mutations conferring tolerance to acetolactate synthase herbicides in soybean
    Article Snippet: .. Expression of Als1 and Als2 mutations Results of the cDNA cloning of the Als1 and Als2 mutations are shown in the 1% Tris-borate-EDTA (TBE) agarose gel in Fig. [30 min at 100 V, 1 kb ladder (NEB Cat. No. N0468S)]. .. The amplified cDNA fragments appear to match the expected fragment sizes of 2066 bp and 1942 bp for Als1 (see Fig. , lanes marked ‘a’ and ‘b’ respectively) and 2083 bp and 1957 bp for Als2 (see Fig. , lanes marked ‘c’ and ‘d’ respectively).

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    New England Biolabs quick load 1 kb dna ladder
    Quick Load 1 Kb Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick load 1 kb dna ladder/product/New England Biolabs
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