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    New England Biolabs low range ssrna ladder
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    range single stranded rna ladder  (New England Biolabs)


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    New England Biolabs range single stranded rna ladder
    Generation of cell lines expressing Drosha truncated in the NTR (A) Schematic diagram of the domain structure of Drosha FL and ΔN-Drosha protein and the human Drosha gene structure (light blue). P: Pro-rich region, R/S: Arg/Ser-rich region, PAZ: Piwi Argonaut and Zwille domain, RIIID: RNase III domain, dsRBD: <t>double-stranded</t> <t>RNA</t> binding domain. (B) RNA-seq data of clones 4, 7, and 8 corresponding to the ex1-8 of the Drosha gene are shown. Each clone was sequenced twice. (C) Total cell lysates of clones 2, 4, 7, 8 or the original HEK293T cells (WT) were subjected to immunoblot analysis of Drosha and GAPDH (loading control). (D) Nuclear (Nuc) and cytoplasmic (Cyto) fraction of +/+ cells (FL) and Δex5/Δex5 cells (ΔN) cells were subjected to immunoblot analysis of Drosha, Lamin A/C (control for the Nuc fraction), and β-actin (control for the Cyto fraction) (top). Relative distribution (%) of FL and ΔN-Drosha in the nucleus vs. cytoplasm is shown (bottom). (E) Co-immunoprecipitation of Drosha (FL or ΔN-Drosha) and Dgcr8 in nuclear extracts of +/+ cells (+) and Δex5/Δex5 cells (Δex5). As control, non-specific IgG (control) was applied. The amount of Drosha after IP is shown. Input samples were subjected to immunoblot analyses of Drosha, Dgcr8, Ddx5, and Lamin A/C (loading control). (F) The level of the Drosha and Dgcr8 mRNA relative to GAPDH mRNA in +/+ cells (black), Δex5/+ cells (blue), and Δex5/Δex5 cells (red) were measured by qRT-PCR and plotted as mean ± SEM. n = 3 independent experiments. See also and <xref ref-type=Figures S1–S3 . " width="250" height="auto" />
    Range Single Stranded Rna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Essential role of the amino-terminal region of Drosha for the Microprocessor function"

    Article Title: Essential role of the amino-terminal region of Drosha for the Microprocessor function

    Journal: iScience

    doi: 10.1016/j.isci.2023.107971

    Generation of cell lines expressing Drosha truncated in the NTR (A) Schematic diagram of the domain structure of Drosha FL and ΔN-Drosha protein and the human Drosha gene structure (light blue). P: Pro-rich region, R/S: Arg/Ser-rich region, PAZ: Piwi Argonaut and Zwille domain, RIIID: RNase III domain, dsRBD: double-stranded RNA binding domain. (B) RNA-seq data of clones 4, 7, and 8 corresponding to the ex1-8 of the Drosha gene are shown. Each clone was sequenced twice. (C) Total cell lysates of clones 2, 4, 7, 8 or the original HEK293T cells (WT) were subjected to immunoblot analysis of Drosha and GAPDH (loading control). (D) Nuclear (Nuc) and cytoplasmic (Cyto) fraction of +/+ cells (FL) and Δex5/Δex5 cells (ΔN) cells were subjected to immunoblot analysis of Drosha, Lamin A/C (control for the Nuc fraction), and β-actin (control for the Cyto fraction) (top). Relative distribution (%) of FL and ΔN-Drosha in the nucleus vs. cytoplasm is shown (bottom). (E) Co-immunoprecipitation of Drosha (FL or ΔN-Drosha) and Dgcr8 in nuclear extracts of +/+ cells (+) and Δex5/Δex5 cells (Δex5). As control, non-specific IgG (control) was applied. The amount of Drosha after IP is shown. Input samples were subjected to immunoblot analyses of Drosha, Dgcr8, Ddx5, and Lamin A/C (loading control). (F) The level of the Drosha and Dgcr8 mRNA relative to GAPDH mRNA in +/+ cells (black), Δex5/+ cells (blue), and Δex5/Δex5 cells (red) were measured by qRT-PCR and plotted as mean ± SEM. n = 3 independent experiments. See also and <xref ref-type=Figures S1–S3 . " title="... Zwille domain, RIIID: RNase III domain, dsRBD: double-stranded RNA binding domain. (B) RNA-seq data of clones 4, ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Generation of cell lines expressing Drosha truncated in the NTR (A) Schematic diagram of the domain structure of Drosha FL and ΔN-Drosha protein and the human Drosha gene structure (light blue). P: Pro-rich region, R/S: Arg/Ser-rich region, PAZ: Piwi Argonaut and Zwille domain, RIIID: RNase III domain, dsRBD: double-stranded RNA binding domain. (B) RNA-seq data of clones 4, 7, and 8 corresponding to the ex1-8 of the Drosha gene are shown. Each clone was sequenced twice. (C) Total cell lysates of clones 2, 4, 7, 8 or the original HEK293T cells (WT) were subjected to immunoblot analysis of Drosha and GAPDH (loading control). (D) Nuclear (Nuc) and cytoplasmic (Cyto) fraction of +/+ cells (FL) and Δex5/Δex5 cells (ΔN) cells were subjected to immunoblot analysis of Drosha, Lamin A/C (control for the Nuc fraction), and β-actin (control for the Cyto fraction) (top). Relative distribution (%) of FL and ΔN-Drosha in the nucleus vs. cytoplasm is shown (bottom). (E) Co-immunoprecipitation of Drosha (FL or ΔN-Drosha) and Dgcr8 in nuclear extracts of +/+ cells (+) and Δex5/Δex5 cells (Δex5). As control, non-specific IgG (control) was applied. The amount of Drosha after IP is shown. Input samples were subjected to immunoblot analyses of Drosha, Dgcr8, Ddx5, and Lamin A/C (loading control). (F) The level of the Drosha and Dgcr8 mRNA relative to GAPDH mRNA in +/+ cells (black), Δex5/+ cells (blue), and Δex5/Δex5 cells (red) were measured by qRT-PCR and plotted as mean ± SEM. n = 3 independent experiments. See also and Figures S1–S3 .

    Techniques Used: Expressing, RNA Binding Assay, RNA Sequencing Assay, Clone Assay, Western Blot, Immunoprecipitation, Quantitative RT-PCR

    ssrna ladder  (New England Biolabs)


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    low range ssrna ladder  (New England Biolabs)


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    low range ssrna ladder  (New England Biolabs)


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    New England Biolabs low range ssrna ladder

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    1) Product Images from "Protocol to measure ribosome density along mRNA transcripts of Arabidopsis thaliana tissues using Ribo-seq"

    Article Title: Protocol to measure ribosome density along mRNA transcripts of Arabidopsis thaliana tissues using Ribo-seq

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2023.102520


    Figure Legend Snippet:

    Techniques Used: Recombinant, Clone Assay, RNA HS Assay, Marker, Modification, Software, Transferring, Sterility, Membrane, Pore Size, Sequencing

    low range ssrna ladder  (New England Biolabs)


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    low range ssrna ladder n0364s  (New England Biolabs)


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    v low range ssrna ladder  (New England Biolabs)


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    Generation of cell lines expressing Drosha truncated in the NTR (A) Schematic diagram of the domain structure of Drosha FL and ΔN-Drosha protein and the human Drosha gene structure (light blue). P: Pro-rich region, R/S: Arg/Ser-rich region, PAZ: Piwi Argonaut and Zwille domain, RIIID: RNase III domain, dsRBD: <t>double-stranded</t> <t>RNA</t> binding domain. (B) RNA-seq data of clones 4, 7, and 8 corresponding to the ex1-8 of the Drosha gene are shown. Each clone was sequenced twice. (C) Total cell lysates of clones 2, 4, 7, 8 or the original HEK293T cells (WT) were subjected to immunoblot analysis of Drosha and GAPDH (loading control). (D) Nuclear (Nuc) and cytoplasmic (Cyto) fraction of +/+ cells (FL) and Δex5/Δex5 cells (ΔN) cells were subjected to immunoblot analysis of Drosha, Lamin A/C (control for the Nuc fraction), and β-actin (control for the Cyto fraction) (top). Relative distribution (%) of FL and ΔN-Drosha in the nucleus vs. cytoplasm is shown (bottom). (E) Co-immunoprecipitation of Drosha (FL or ΔN-Drosha) and Dgcr8 in nuclear extracts of +/+ cells (+) and Δex5/Δex5 cells (Δex5). As control, non-specific IgG (control) was applied. The amount of Drosha after IP is shown. Input samples were subjected to immunoblot analyses of Drosha, Dgcr8, Ddx5, and Lamin A/C (loading control). (F) The level of the Drosha and Dgcr8 mRNA relative to GAPDH mRNA in +/+ cells (black), Δex5/+ cells (blue), and Δex5/Δex5 cells (red) were measured by qRT-PCR and plotted as mean ± SEM. n = 3 independent experiments. See also and <xref ref-type=Figures S1–S3 . " width="250" height="auto" />
    Range Single Stranded Rna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Generation of cell lines expressing Drosha truncated in the NTR (A) Schematic diagram of the domain structure of Drosha FL and ΔN-Drosha protein and the human Drosha gene structure (light blue). P: Pro-rich region, R/S: Arg/Ser-rich region, PAZ: Piwi Argonaut and Zwille domain, RIIID: RNase III domain, dsRBD: <t>double-stranded</t> <t>RNA</t> binding domain. (B) RNA-seq data of clones 4, 7, and 8 corresponding to the ex1-8 of the Drosha gene are shown. Each clone was sequenced twice. (C) Total cell lysates of clones 2, 4, 7, 8 or the original HEK293T cells (WT) were subjected to immunoblot analysis of Drosha and GAPDH (loading control). (D) Nuclear (Nuc) and cytoplasmic (Cyto) fraction of +/+ cells (FL) and Δex5/Δex5 cells (ΔN) cells were subjected to immunoblot analysis of Drosha, Lamin A/C (control for the Nuc fraction), and β-actin (control for the Cyto fraction) (top). Relative distribution (%) of FL and ΔN-Drosha in the nucleus vs. cytoplasm is shown (bottom). (E) Co-immunoprecipitation of Drosha (FL or ΔN-Drosha) and Dgcr8 in nuclear extracts of +/+ cells (+) and Δex5/Δex5 cells (Δex5). As control, non-specific IgG (control) was applied. The amount of Drosha after IP is shown. Input samples were subjected to immunoblot analyses of Drosha, Dgcr8, Ddx5, and Lamin A/C (loading control). (F) The level of the Drosha and Dgcr8 mRNA relative to GAPDH mRNA in +/+ cells (black), Δex5/+ cells (blue), and Δex5/Δex5 cells (red) were measured by qRT-PCR and plotted as mean ± SEM. n = 3 independent experiments. See also and <xref ref-type=Figures S1–S3 . " width="250" height="auto" />
    Ssrna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Generation of cell lines expressing Drosha truncated in the NTR (A) Schematic diagram of the domain structure of Drosha FL and ΔN-Drosha protein and the human Drosha gene structure (light blue). P: Pro-rich region, R/S: Arg/Ser-rich region, PAZ: Piwi Argonaut and Zwille domain, RIIID: RNase III domain, dsRBD: <t>double-stranded</t> <t>RNA</t> binding domain. (B) RNA-seq data of clones 4, 7, and 8 corresponding to the ex1-8 of the Drosha gene are shown. Each clone was sequenced twice. (C) Total cell lysates of clones 2, 4, 7, 8 or the original HEK293T cells (WT) were subjected to immunoblot analysis of Drosha and GAPDH (loading control). (D) Nuclear (Nuc) and cytoplasmic (Cyto) fraction of +/+ cells (FL) and Δex5/Δex5 cells (ΔN) cells were subjected to immunoblot analysis of Drosha, Lamin A/C (control for the Nuc fraction), and β-actin (control for the Cyto fraction) (top). Relative distribution (%) of FL and ΔN-Drosha in the nucleus vs. cytoplasm is shown (bottom). (E) Co-immunoprecipitation of Drosha (FL or ΔN-Drosha) and Dgcr8 in nuclear extracts of +/+ cells (+) and Δex5/Δex5 cells (Δex5). As control, non-specific IgG (control) was applied. The amount of Drosha after IP is shown. Input samples were subjected to immunoblot analyses of Drosha, Dgcr8, Ddx5, and Lamin A/C (loading control). (F) The level of the Drosha and Dgcr8 mRNA relative to GAPDH mRNA in +/+ cells (black), Δex5/+ cells (blue), and Δex5/Δex5 cells (red) were measured by qRT-PCR and plotted as mean ± SEM. n = 3 independent experiments. See also and <xref ref-type=Figures S1–S3 . " width="250" height="auto" />
    Low Range Ssrna Ladder N0364s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Generation of cell lines expressing Drosha truncated in the NTR (A) Schematic diagram of the domain structure of Drosha FL and ΔN-Drosha protein and the human Drosha gene structure (light blue). P: Pro-rich region, R/S: Arg/Ser-rich region, PAZ: Piwi Argonaut and Zwille domain, RIIID: RNase III domain, dsRBD: <t>double-stranded</t> <t>RNA</t> binding domain. (B) RNA-seq data of clones 4, 7, and 8 corresponding to the ex1-8 of the Drosha gene are shown. Each clone was sequenced twice. (C) Total cell lysates of clones 2, 4, 7, 8 or the original HEK293T cells (WT) were subjected to immunoblot analysis of Drosha and GAPDH (loading control). (D) Nuclear (Nuc) and cytoplasmic (Cyto) fraction of +/+ cells (FL) and Δex5/Δex5 cells (ΔN) cells were subjected to immunoblot analysis of Drosha, Lamin A/C (control for the Nuc fraction), and β-actin (control for the Cyto fraction) (top). Relative distribution (%) of FL and ΔN-Drosha in the nucleus vs. cytoplasm is shown (bottom). (E) Co-immunoprecipitation of Drosha (FL or ΔN-Drosha) and Dgcr8 in nuclear extracts of +/+ cells (+) and Δex5/Δex5 cells (Δex5). As control, non-specific IgG (control) was applied. The amount of Drosha after IP is shown. Input samples were subjected to immunoblot analyses of Drosha, Dgcr8, Ddx5, and Lamin A/C (loading control). (F) The level of the Drosha and Dgcr8 mRNA relative to GAPDH mRNA in +/+ cells (black), Δex5/+ cells (blue), and Δex5/Δex5 cells (red) were measured by qRT-PCR and plotted as mean ± SEM. n = 3 independent experiments. See also and <xref ref-type=Figures S1–S3 . " width="250" height="auto" />
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    Generation of cell lines expressing Drosha truncated in the NTR (A) Schematic diagram of the domain structure of Drosha FL and ΔN-Drosha protein and the human Drosha gene structure (light blue). P: Pro-rich region, R/S: Arg/Ser-rich region, PAZ: Piwi Argonaut and Zwille domain, RIIID: RNase III domain, dsRBD: double-stranded RNA binding domain. (B) RNA-seq data of clones 4, 7, and 8 corresponding to the ex1-8 of the Drosha gene are shown. Each clone was sequenced twice. (C) Total cell lysates of clones 2, 4, 7, 8 or the original HEK293T cells (WT) were subjected to immunoblot analysis of Drosha and GAPDH (loading control). (D) Nuclear (Nuc) and cytoplasmic (Cyto) fraction of +/+ cells (FL) and Δex5/Δex5 cells (ΔN) cells were subjected to immunoblot analysis of Drosha, Lamin A/C (control for the Nuc fraction), and β-actin (control for the Cyto fraction) (top). Relative distribution (%) of FL and ΔN-Drosha in the nucleus vs. cytoplasm is shown (bottom). (E) Co-immunoprecipitation of Drosha (FL or ΔN-Drosha) and Dgcr8 in nuclear extracts of +/+ cells (+) and Δex5/Δex5 cells (Δex5). As control, non-specific IgG (control) was applied. The amount of Drosha after IP is shown. Input samples were subjected to immunoblot analyses of Drosha, Dgcr8, Ddx5, and Lamin A/C (loading control). (F) The level of the Drosha and Dgcr8 mRNA relative to GAPDH mRNA in +/+ cells (black), Δex5/+ cells (blue), and Δex5/Δex5 cells (red) were measured by qRT-PCR and plotted as mean ± SEM. n = 3 independent experiments. See also and <xref ref-type=Figures S1–S3 . " width="100%" height="100%">

    Journal: iScience

    Article Title: Essential role of the amino-terminal region of Drosha for the Microprocessor function

    doi: 10.1016/j.isci.2023.107971

    Figure Lengend Snippet: Generation of cell lines expressing Drosha truncated in the NTR (A) Schematic diagram of the domain structure of Drosha FL and ΔN-Drosha protein and the human Drosha gene structure (light blue). P: Pro-rich region, R/S: Arg/Ser-rich region, PAZ: Piwi Argonaut and Zwille domain, RIIID: RNase III domain, dsRBD: double-stranded RNA binding domain. (B) RNA-seq data of clones 4, 7, and 8 corresponding to the ex1-8 of the Drosha gene are shown. Each clone was sequenced twice. (C) Total cell lysates of clones 2, 4, 7, 8 or the original HEK293T cells (WT) were subjected to immunoblot analysis of Drosha and GAPDH (loading control). (D) Nuclear (Nuc) and cytoplasmic (Cyto) fraction of +/+ cells (FL) and Δex5/Δex5 cells (ΔN) cells were subjected to immunoblot analysis of Drosha, Lamin A/C (control for the Nuc fraction), and β-actin (control for the Cyto fraction) (top). Relative distribution (%) of FL and ΔN-Drosha in the nucleus vs. cytoplasm is shown (bottom). (E) Co-immunoprecipitation of Drosha (FL or ΔN-Drosha) and Dgcr8 in nuclear extracts of +/+ cells (+) and Δex5/Δex5 cells (Δex5). As control, non-specific IgG (control) was applied. The amount of Drosha after IP is shown. Input samples were subjected to immunoblot analyses of Drosha, Dgcr8, Ddx5, and Lamin A/C (loading control). (F) The level of the Drosha and Dgcr8 mRNA relative to GAPDH mRNA in +/+ cells (black), Δex5/+ cells (blue), and Δex5/Δex5 cells (red) were measured by qRT-PCR and plotted as mean ± SEM. n = 3 independent experiments. See also and Figures S1–S3 .

    Article Snippet: The low range single stranded RNA ladder (#N0364N, NEB) was stained with SYBR Gold (#S11494, Thermo Fisher Scientific) according to the manufacturer’s protocol and used as a molecular marker.

    Techniques: Expressing, RNA Binding Assay, RNA Sequencing Assay, Clone Assay, Western Blot, Immunoprecipitation, Quantitative RT-PCR