1 nourseothricin sulfate  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc 1 nourseothricin sulfate
    1 Nourseothricin Sulfate, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1 nourseothricin sulfate  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc 1 nourseothricin sulfate
    1 Nourseothricin Sulfate, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nourseothricin  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc nourseothricin
    Nourseothricin, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nourseothricin sulfate  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc nourseothricin sulfate
    Nourseothricin Sulfate, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nourseothricin sulfate  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc nourseothricin sulfate
    Nourseothricin Sulfate, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nourseothricin sulfate  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc nourseothricin sulfate
    Nourseothricin Sulfate, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nourseothricin sulfate  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc nourseothricin sulfate
    Nourseothricin Sulfate, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    n 500 3  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc n 500 3
    N 500 3, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nourseothricin sulfate clonnat  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc nourseothricin sulfate clonnat

    Nourseothricin Sulfate Clonnat, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Enhanced fluorescent imaging of proteins in live yeast cells using fluorescently labeled scFv"

    Article Title: Enhanced fluorescent imaging of proteins in live yeast cells using fluorescently labeled scFv

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2023.102299


    Figure Legend Snippet:

    Techniques Used: Recombinant, Staining, Microscopy, Agarose Gel Electrophoresis, Labeling, Amplification, Plasmid Preparation, Expressing, Software, Stripping Membranes, Transferring, Imaging, Spectrophotometry

    streptothricin compounds  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc streptothricin compounds
    Streptothricin Compounds, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nourseothricin sulfate  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc nourseothricin sulfate
    Streptothricins share streptolidine and carbamoylated gulosamine sugar moieties. They are distinguished by differing numbers of β-lysines attached end-to-end through amide bonds to the ε-amino groups. <t>Nourseothricin</t> is the natural product mixture of several streptothricins, predominantly S-F (1 β-lysine) and S-D (3 β-lysines). Acetylation of the β-amino group blocks activity and is the major known mechanism of antimicrobial resistance to streptothricins. S-D, streptothricin D; S-F, streptothricin-F.
    Nourseothricin Sulfate, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Streptothricin F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosome"

    Article Title: Streptothricin F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosome

    Journal: PLOS Biology

    doi: 10.1371/journal.pbio.3002091

    Streptothricins share streptolidine and carbamoylated gulosamine sugar moieties. They are distinguished by differing numbers of β-lysines attached end-to-end through amide bonds to the ε-amino groups. Nourseothricin is the natural product mixture of several streptothricins, predominantly S-F (1 β-lysine) and S-D (3 β-lysines). Acetylation of the β-amino group blocks activity and is the major known mechanism of antimicrobial resistance to streptothricins. S-D, streptothricin D; S-F, streptothricin-F.
    Figure Legend Snippet: Streptothricins share streptolidine and carbamoylated gulosamine sugar moieties. They are distinguished by differing numbers of β-lysines attached end-to-end through amide bonds to the ε-amino groups. Nourseothricin is the natural product mixture of several streptothricins, predominantly S-F (1 β-lysine) and S-D (3 β-lysines). Acetylation of the β-amino group blocks activity and is the major known mechanism of antimicrobial resistance to streptothricins. S-D, streptothricin D; S-F, streptothricin-F.

    Techniques Used: Activity Assay

    (A) Nourseothricin MIC 0.25 μM. (B) S-F MIC 1 μM. Data are available in . MIC, minimal inhibitory concentration; S-F, streptothricin-F.
    Figure Legend Snippet: (A) Nourseothricin MIC 0.25 μM. (B) S-F MIC 1 μM. Data are available in . MIC, minimal inhibitory concentration; S-F, streptothricin-F.

    Techniques Used: Concentration Assay

    (A) Inhibition of prokaryotic in vitro translation using coupled in vitro transcription–translation extracts with readout from a nanoluciferase reporter. (B) Inhibition of eukaryotic in vitro translation using coupled in vitro transcription–translation extracts with readout from a nanoluciferase reporter. S-F, S-D, NTC, APR, and TET data represent mean and standard deviation from 3 independent experiments. Data are available in . APR, apramycin; NTC, nourseothricin; S-D, streptothricin D; S-F, streptothricin-F; TET, tetracycline.
    Figure Legend Snippet: (A) Inhibition of prokaryotic in vitro translation using coupled in vitro transcription–translation extracts with readout from a nanoluciferase reporter. (B) Inhibition of eukaryotic in vitro translation using coupled in vitro transcription–translation extracts with readout from a nanoluciferase reporter. S-F, S-D, NTC, APR, and TET data represent mean and standard deviation from 3 independent experiments. Data are available in . APR, apramycin; NTC, nourseothricin; S-D, streptothricin D; S-F, streptothricin-F; TET, tetracycline.

    Techniques Used: Inhibition, In Vitro, Standard Deviation

    J774 macrophages and LLC-PK-1 renal epithelial cells were treated with 2-fold doubling dilutions of nourseothricin, S-D, and S-F for up to 5 days in the presence of SYTOX-Green. SYTOX-Green is a cell membrane-impermeant nucleic acid binding dye that fluoresces on binding to nuclear DNA. It therefore provides a real-time readout of eukaryotic cell membrane permeabilization associated with cell death that can be continuously monitored through fluorescence measurements. Cytotoxicity was minimal to absent after a single day incubation but increased on subsequent days. Nourseothricin and S-D effects were essentially indistinguishable. S-F toxicity was only observed at molar concentration at least 10-fold greater than S-D beginning at 32 μM, significantly above MIC ranges observed in activity spectrum analysis. Each data point represents mean and standard deviation for assays performed in quadruplicate. Data are available in . MIC, minimal inhibitory concentration; S-D, streptothricin D; S-F, streptothricin-F.
    Figure Legend Snippet: J774 macrophages and LLC-PK-1 renal epithelial cells were treated with 2-fold doubling dilutions of nourseothricin, S-D, and S-F for up to 5 days in the presence of SYTOX-Green. SYTOX-Green is a cell membrane-impermeant nucleic acid binding dye that fluoresces on binding to nuclear DNA. It therefore provides a real-time readout of eukaryotic cell membrane permeabilization associated with cell death that can be continuously monitored through fluorescence measurements. Cytotoxicity was minimal to absent after a single day incubation but increased on subsequent days. Nourseothricin and S-D effects were essentially indistinguishable. S-F toxicity was only observed at molar concentration at least 10-fold greater than S-D beginning at 32 μM, significantly above MIC ranges observed in activity spectrum analysis. Each data point represents mean and standard deviation for assays performed in quadruplicate. Data are available in . MIC, minimal inhibitory concentration; S-D, streptothricin D; S-F, streptothricin-F.

    Techniques Used: Binding Assay, Fluorescence, Incubation, Concentration Assay, Activity Assay, Standard Deviation

    (A) S-F dosing at 100 mg/kg without obvious histological abnormality in kidney. (B) Nourseothricin dosing at 10 mg/kg showing cellular necrosis and nuclear degeneration of proximal convoluted tubule epithelial cells (arrowheads). Glomeruli and distal tubules were spared. Tissue was harvested 3 days after dosing. Size bar = 20 μM.
    Figure Legend Snippet: (A) S-F dosing at 100 mg/kg without obvious histological abnormality in kidney. (B) Nourseothricin dosing at 10 mg/kg showing cellular necrosis and nuclear degeneration of proximal convoluted tubule epithelial cells (arrowheads). Glomeruli and distal tubules were spared. Tissue was harvested 3 days after dosing. Size bar = 20 μM.

    Techniques Used:

    Modal MIC values for resistance mutants identified in single rrn operon E . coli strain identifies unique target in helix 34 of ribosomal 16S rRNA.
    Figure Legend Snippet: Modal MIC values for resistance mutants identified in single rrn operon E . coli strain identifies unique target in helix 34 of ribosomal 16S rRNA.

    Techniques Used:

    TetM ribosomal protection protein does not interfere with nourseothricin activity.
    Figure Legend Snippet: TetM ribosomal protection protein does not interfere with nourseothricin activity.

    Techniques Used: Activity Assay

    (A, B) Location of the S-F and S-D binding sites in the A . baumannii 70S ribosome with P-site tRNA, respectively. (C, D) Hydrogen-bonding interactions between the streptolidine moieties of S-F and S-D and C1054 of the 16S rRNA, respectively (E, F) Electrostatic interactions of the S-F and S-D 12-carbamoylated gulosamine moiety with A1196, respectively. (G, H) Electrostatic interactions between β-amino of the β-lysine and the O2’ atoms of C1054 and U1052. Throughout the figure, the 50S is blue, 30S is yellow, S-F is gray sticks, S-D is green sticks, and highlighted residues are cyan. The cryo-EM densities corresponding to the 2 terminal β-lysine moieties of S-D were very weak, indicating that these 2 lysine moieties are flexible. We therefore did not include these 2 terminal β-lysine moieties in panels D, F, and H. cryo-EM, cryo-electron microscopy; S-D, streptothricin D; S-F, streptothricin-F.
    Figure Legend Snippet: (A, B) Location of the S-F and S-D binding sites in the A . baumannii 70S ribosome with P-site tRNA, respectively. (C, D) Hydrogen-bonding interactions between the streptolidine moieties of S-F and S-D and C1054 of the 16S rRNA, respectively (E, F) Electrostatic interactions of the S-F and S-D 12-carbamoylated gulosamine moiety with A1196, respectively. (G, H) Electrostatic interactions between β-amino of the β-lysine and the O2’ atoms of C1054 and U1052. Throughout the figure, the 50S is blue, 30S is yellow, S-F is gray sticks, S-D is green sticks, and highlighted residues are cyan. The cryo-EM densities corresponding to the 2 terminal β-lysine moieties of S-D were very weak, indicating that these 2 lysine moieties are flexible. We therefore did not include these 2 terminal β-lysine moieties in panels D, F, and H. cryo-EM, cryo-electron microscopy; S-D, streptothricin D; S-F, streptothricin-F.

    Techniques Used: Binding Assay, Cryo-EM Sample Prep, Electron Microscopy

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    Gold Biotechnology Inc 1 nourseothricin sulfate
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    nourseothricin  (Gold Biotechnology Inc)
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    n 500 3  (Gold Biotechnology Inc)
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    nourseothricin sulfate clonnat  (Gold Biotechnology Inc)
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    Journal: STAR Protocols

    Article Title: Enhanced fluorescent imaging of proteins in live yeast cells using fluorescently labeled scFv

    doi: 10.1016/j.xpro.2023.102299

    Figure Lengend Snippet:

    Article Snippet: Nourseothricin sulfate (ClonNAT) (powder) , GoldBio , Cat# N-500-3.

    Techniques: Recombinant, Staining, Microscopy, Agarose Gel Electrophoresis, Labeling, Amplification, Plasmid Preparation, Expressing, Software, Stripping Membranes, Transferring, Imaging, Spectrophotometry