proteases  (Alomone Labs)


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    Structured Review

    Alomone Labs proteases
    Proteases, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteases/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    proteases - by Bioz Stars, 2022-09
    94/100 stars

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  • 93
    Alomone Labs activator ns19504
    BK channel activator <t>NS19504</t> promoted microglial phagocytosis after OGD in primary culture . (A) Representative images of beads (red) phagocytosed by CD11b + microglia (green) after 1 h of OGD. Control group: primary microglia cultured with basic microglia medium; NS19504 group: primary microglia cultured with 10 μM NS19504 in basic microglia medium; Paxilline group: primary microglia cultured with 1 μM Paxilline in basic microglia medium. Scale bar = 25 μm. (B) Bar graph showed that semiquantitative data of the relative bead density versus microglia. Data are mean + SE, n = 3 per group. * p
    Activator Ns19504, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    activator ns19504 - by Bioz Stars, 2022-09
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    94
    Alomone Labs cleavage resistant prongf
    <t>Overexpression</t> of <t>proNGF</t> induced glial Müller activation and inflammation. Immunohistochemical analysis of retinas was performed 4 weeks after intravitreal injections of pGFP alone (control) ( a–e ), proNGF plus scrambled p75 NTR shRNA (proNGF+Scr)
    Cleavage Resistant Prongf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    93
    Alomone Labs penitrem a
    The identification of K + channels in setting HCASMC resting membrane potential. (A–F) The change in the fractional fluorescence of DiBAC 4 (3) treated with 10 µM glibenclamide (A), 200 nM <t>penitrem</t> A (B), 1 µM tram34 (C), 200 nM apamin (D), 25 µM BaCl 2 (E), and 10 µM XE991 (F). (G) A summary of percentchange in fractional fluorescence of DiBAC 4 (3) after application of K + channel inhibitors: glibenclamide ( p
    Penitrem A, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    BK channel activator NS19504 promoted microglial phagocytosis after OGD in primary culture . (A) Representative images of beads (red) phagocytosed by CD11b + microglia (green) after 1 h of OGD. Control group: primary microglia cultured with basic microglia medium; NS19504 group: primary microglia cultured with 10 μM NS19504 in basic microglia medium; Paxilline group: primary microglia cultured with 1 μM Paxilline in basic microglia medium. Scale bar = 25 μm. (B) Bar graph showed that semiquantitative data of the relative bead density versus microglia. Data are mean + SE, n = 3 per group. * p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: BK Channel-Mediated Microglial Phagocytosis Alleviates Neurological Deficit After Ischemic Stroke

    doi: 10.3389/fncel.2021.683769

    Figure Lengend Snippet: BK channel activator NS19504 promoted microglial phagocytosis after OGD in primary culture . (A) Representative images of beads (red) phagocytosed by CD11b + microglia (green) after 1 h of OGD. Control group: primary microglia cultured with basic microglia medium; NS19504 group: primary microglia cultured with 10 μM NS19504 in basic microglia medium; Paxilline group: primary microglia cultured with 1 μM Paxilline in basic microglia medium. Scale bar = 25 μm. (B) Bar graph showed that semiquantitative data of the relative bead density versus microglia. Data are mean + SE, n = 3 per group. * p

    Article Snippet: Antagonist and Activator Administration The selective BK channel antagonist Paxilline ( ) and activator NS19504 ( ) powder (Alomone Labs, Jerusalem, Israel) were, respectively, dissolved in DMSO to the final storage concentrations of 2 and 50 mM.

    Techniques: Cell Culture

    Activation of BK channels ameliorated stroke outcomes. (A) Experiment design of the animal experiment. Three days before tMCAO, mice were subjected behavior training. After a 90-min tMCAO surgery, mice were divided randomly into four groups (sham, DMSO, NS19504, and Paxilline). At 1, 3, 7, and 14 days, behavior tests were performed, and protein and RNA samples were collected. (B) Weight changes of mice in three groups at 1, 2, 3, 7, and 14 days after tMCAO. (C) Modified Neurological Severity Score of three groups at 1, 3, 7, and 14 days after tMCAO. (D) Average score per second of three groups of hinging wire at 3 days after tMCAO. (E) Survivorship curve during 14 days of three groups after tMCAO. Data are mean + SE, n = 21–23 per group at 1 day, n = 17–23 per group at 3 days, n = 8–13 per group at 7 days, n = 3–9 per group at 14 days. *NS19504 vs. Paxilline group, ** p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: BK Channel-Mediated Microglial Phagocytosis Alleviates Neurological Deficit After Ischemic Stroke

    doi: 10.3389/fncel.2021.683769

    Figure Lengend Snippet: Activation of BK channels ameliorated stroke outcomes. (A) Experiment design of the animal experiment. Three days before tMCAO, mice were subjected behavior training. After a 90-min tMCAO surgery, mice were divided randomly into four groups (sham, DMSO, NS19504, and Paxilline). At 1, 3, 7, and 14 days, behavior tests were performed, and protein and RNA samples were collected. (B) Weight changes of mice in three groups at 1, 2, 3, 7, and 14 days after tMCAO. (C) Modified Neurological Severity Score of three groups at 1, 3, 7, and 14 days after tMCAO. (D) Average score per second of three groups of hinging wire at 3 days after tMCAO. (E) Survivorship curve during 14 days of three groups after tMCAO. Data are mean + SE, n = 21–23 per group at 1 day, n = 17–23 per group at 3 days, n = 8–13 per group at 7 days, n = 3–9 per group at 14 days. *NS19504 vs. Paxilline group, ** p

    Article Snippet: Antagonist and Activator Administration The selective BK channel antagonist Paxilline ( ) and activator NS19504 ( ) powder (Alomone Labs, Jerusalem, Israel) were, respectively, dissolved in DMSO to the final storage concentrations of 2 and 50 mM.

    Techniques: Activation Assay, Mouse Assay, Modification

    Activation of BK channels promoted the phagocytic function of microglia after tMCAO and decreased neuronal apoptosis . (A) Representative photomicrograms of IBA1 + microglia (green) and TUNEL + apoptotic cells (red) in the peri-infarct region of brain slice in the DMSO, NS19504, and Paxilline groups at 3 days after tMCAO. Scale bar = 50 μm. (B) Bar graph shows quantitative analysis of the number of IBA1 + TUNEL + cells per field. Data are mean + SE, n = 3 per group. *vs. DMSO group, ** p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: BK Channel-Mediated Microglial Phagocytosis Alleviates Neurological Deficit After Ischemic Stroke

    doi: 10.3389/fncel.2021.683769

    Figure Lengend Snippet: Activation of BK channels promoted the phagocytic function of microglia after tMCAO and decreased neuronal apoptosis . (A) Representative photomicrograms of IBA1 + microglia (green) and TUNEL + apoptotic cells (red) in the peri-infarct region of brain slice in the DMSO, NS19504, and Paxilline groups at 3 days after tMCAO. Scale bar = 50 μm. (B) Bar graph shows quantitative analysis of the number of IBA1 + TUNEL + cells per field. Data are mean + SE, n = 3 per group. *vs. DMSO group, ** p

    Article Snippet: Antagonist and Activator Administration The selective BK channel antagonist Paxilline ( ) and activator NS19504 ( ) powder (Alomone Labs, Jerusalem, Israel) were, respectively, dissolved in DMSO to the final storage concentrations of 2 and 50 mM.

    Techniques: Activation Assay, TUNEL Assay, Slice Preparation

    Inflammatory factors and phosphorylated-ERK expressions under different treatments at 3 days after tMCAO . (A) Bar graph represents the relative mRNA levels of inflammatory factors including TNF-α, TGF-β, IL-10, IL-6, IL-1β, and IL-1α. Data are mean + SE, n = 3 per group. (B) Representative image of Western blot of phosphorylated-ERK1/2 (p-ERK), total-ERK1/2 (t-ERK), and β-actin at 3 days after tMCAO. (C) Bar graph showing the relative protein levels of p -ERK vs. t-ERK at 3 day after tMCAO. Data are mean + SE, n = 9 per group. *NS19504 vs. Paxilline group, * p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: BK Channel-Mediated Microglial Phagocytosis Alleviates Neurological Deficit After Ischemic Stroke

    doi: 10.3389/fncel.2021.683769

    Figure Lengend Snippet: Inflammatory factors and phosphorylated-ERK expressions under different treatments at 3 days after tMCAO . (A) Bar graph represents the relative mRNA levels of inflammatory factors including TNF-α, TGF-β, IL-10, IL-6, IL-1β, and IL-1α. Data are mean + SE, n = 3 per group. (B) Representative image of Western blot of phosphorylated-ERK1/2 (p-ERK), total-ERK1/2 (t-ERK), and β-actin at 3 days after tMCAO. (C) Bar graph showing the relative protein levels of p -ERK vs. t-ERK at 3 day after tMCAO. Data are mean + SE, n = 9 per group. *NS19504 vs. Paxilline group, * p

    Article Snippet: Antagonist and Activator Administration The selective BK channel antagonist Paxilline ( ) and activator NS19504 ( ) powder (Alomone Labs, Jerusalem, Israel) were, respectively, dissolved in DMSO to the final storage concentrations of 2 and 50 mM.

    Techniques: Western Blot

    Overexpression of proNGF induced glial Müller activation and inflammation. Immunohistochemical analysis of retinas was performed 4 weeks after intravitreal injections of pGFP alone (control) ( a–e ), proNGF plus scrambled p75 NTR shRNA (proNGF+Scr)

    Journal: Diabetologia

    Article Title: Modulation of p75NTR prevents diabetes- and proNGF-induced retinal inflammation and blood–retina barrier breakdown in mice and rats

    doi: 10.1007/s00125-013-2998-6

    Figure Lengend Snippet: Overexpression of proNGF induced glial Müller activation and inflammation. Immunohistochemical analysis of retinas was performed 4 weeks after intravitreal injections of pGFP alone (control) ( a–e ), proNGF plus scrambled p75 NTR shRNA (proNGF+Scr)

    Article Snippet: Representative blots ( ) and statistical analysis show that overexpression of cleavage-resistant proNGF plus scrambled shRNA induced a 2.5-fold increase in proNGF , a 1.8-fold increase in p75 , a 3.6-fold increase in NFκB , a 1.7-fold increase in TNF-α ( ) and a 2.3-fold increase in IL-1β ( ).

    Techniques: Over Expression, Activation Assay, Immunohistochemistry, shRNA

    The identification of K + channels in setting HCASMC resting membrane potential. (A–F) The change in the fractional fluorescence of DiBAC 4 (3) treated with 10 µM glibenclamide (A), 200 nM penitrem A (B), 1 µM tram34 (C), 200 nM apamin (D), 25 µM BaCl 2 (E), and 10 µM XE991 (F). (G) A summary of percentchange in fractional fluorescence of DiBAC 4 (3) after application of K + channel inhibitors: glibenclamide ( p

    Journal: PeerJ

    Article Title: Hypoxia and metabolic inhibitors alter the intracellular ATP:ADP ratio and membrane potential in human coronary artery smooth muscle cells

    doi: 10.7717/peerj.10344

    Figure Lengend Snippet: The identification of K + channels in setting HCASMC resting membrane potential. (A–F) The change in the fractional fluorescence of DiBAC 4 (3) treated with 10 µM glibenclamide (A), 200 nM penitrem A (B), 1 µM tram34 (C), 200 nM apamin (D), 25 µM BaCl 2 (E), and 10 µM XE991 (F). (G) A summary of percentchange in fractional fluorescence of DiBAC 4 (3) after application of K + channel inhibitors: glibenclamide ( p

    Article Snippet: However, exposure to hypoxia resulted in significant decrease in DiBAC4 (3) fluorescence when in the presence of 10 µM pinacidil (n = 5), 10 µM glibenclamide (n = 36), 25 µM BaCl2 (n = 14), 100 nM IbTX (n = 17), 200 nM penitrem A (n = 14), and 10 µM glibenclamide plus 25 µM BaCl2 (n = 25) ( ).

    Techniques: Fluorescence