ngf  (Alomone Labs)


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    Alomone Labs ngf
    Negr1 exerts a trophic effect on sympathetic neurons. a Immunofluoescence images showing Negr1 and TYR.H. distribution in wt and ob/ob TH PVAT sections (from 1 out of 2 replicates). Insets show higher magnification of framed areas. Nuclei are counterstained with DAPI. Scale bar: 50 µm. b Representative micrographs of SCG sympathetic neurons cultured at low density for 24 h in presence of 1 or 10 ng/ml <t>NGF,</t> with or without 500 ng/ml recombinant Negr1, and stained for β3-tubulin. Scale bar: 100 µm. c Quantifications of total axonal length, branching points and principal neurites on primary sympathetic neurons incubated for 24 h in medium supplemented with the indicated factors. Violin plots show median and quartiles of 80
    Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngf/product/Alomone Labs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ngf - by Bioz Stars, 2022-12
    94/100 stars

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    1) Product Images from "Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity"

    Article Title: Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity

    Journal: Nature Communications

    doi: 10.1038/s41467-022-34846-w

    Negr1 exerts a trophic effect on sympathetic neurons. a Immunofluoescence images showing Negr1 and TYR.H. distribution in wt and ob/ob TH PVAT sections (from 1 out of 2 replicates). Insets show higher magnification of framed areas. Nuclei are counterstained with DAPI. Scale bar: 50 µm. b Representative micrographs of SCG sympathetic neurons cultured at low density for 24 h in presence of 1 or 10 ng/ml NGF, with or without 500 ng/ml recombinant Negr1, and stained for β3-tubulin. Scale bar: 100 µm. c Quantifications of total axonal length, branching points and principal neurites on primary sympathetic neurons incubated for 24 h in medium supplemented with the indicated factors. Violin plots show median and quartiles of 80
    Figure Legend Snippet: Negr1 exerts a trophic effect on sympathetic neurons. a Immunofluoescence images showing Negr1 and TYR.H. distribution in wt and ob/ob TH PVAT sections (from 1 out of 2 replicates). Insets show higher magnification of framed areas. Nuclei are counterstained with DAPI. Scale bar: 50 µm. b Representative micrographs of SCG sympathetic neurons cultured at low density for 24 h in presence of 1 or 10 ng/ml NGF, with or without 500 ng/ml recombinant Negr1, and stained for β3-tubulin. Scale bar: 100 µm. c Quantifications of total axonal length, branching points and principal neurites on primary sympathetic neurons incubated for 24 h in medium supplemented with the indicated factors. Violin plots show median and quartiles of 80

    Techniques Used: Cell Culture, Recombinant, Staining, Incubation

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    Alomone Labs ngf
    Negr1 exerts a trophic effect on sympathetic neurons. a Immunofluoescence images showing Negr1 and TYR.H. distribution in wt and ob/ob TH PVAT sections (from 1 out of 2 replicates). Insets show higher magnification of framed areas. Nuclei are counterstained with DAPI. Scale bar: 50 µm. b Representative micrographs of SCG sympathetic neurons cultured at low density for 24 h in presence of 1 or 10 ng/ml <t>NGF,</t> with or without 500 ng/ml recombinant Negr1, and stained for β3-tubulin. Scale bar: 100 µm. c Quantifications of total axonal length, branching points and principal neurites on primary sympathetic neurons incubated for 24 h in medium supplemented with the indicated factors. Violin plots show median and quartiles of 80
    Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngf/product/Alomone Labs
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    ngf - by Bioz Stars, 2022-12
    94/100 stars
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    Negr1 exerts a trophic effect on sympathetic neurons. a Immunofluoescence images showing Negr1 and TYR.H. distribution in wt and ob/ob TH PVAT sections (from 1 out of 2 replicates). Insets show higher magnification of framed areas. Nuclei are counterstained with DAPI. Scale bar: 50 µm. b Representative micrographs of SCG sympathetic neurons cultured at low density for 24 h in presence of 1 or 10 ng/ml NGF, with or without 500 ng/ml recombinant Negr1, and stained for β3-tubulin. Scale bar: 100 µm. c Quantifications of total axonal length, branching points and principal neurites on primary sympathetic neurons incubated for 24 h in medium supplemented with the indicated factors. Violin plots show median and quartiles of 80

    Journal: Nature Communications

    Article Title: Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity

    doi: 10.1038/s41467-022-34846-w

    Figure Lengend Snippet: Negr1 exerts a trophic effect on sympathetic neurons. a Immunofluoescence images showing Negr1 and TYR.H. distribution in wt and ob/ob TH PVAT sections (from 1 out of 2 replicates). Insets show higher magnification of framed areas. Nuclei are counterstained with DAPI. Scale bar: 50 µm. b Representative micrographs of SCG sympathetic neurons cultured at low density for 24 h in presence of 1 or 10 ng/ml NGF, with or without 500 ng/ml recombinant Negr1, and stained for β3-tubulin. Scale bar: 100 µm. c Quantifications of total axonal length, branching points and principal neurites on primary sympathetic neurons incubated for 24 h in medium supplemented with the indicated factors. Violin plots show median and quartiles of 80

    Article Snippet: Neurons were grown for 24 h in complete medium supplemented with 1 or 10 ng/ml NGF (nerve growth factor, Alomone Labs) with or without the indicated doses of murine recombinant Negr1 (Sino Biological), before being fixed for immunofluorescence.

    Techniques: Cell Culture, Recombinant, Staining, Incubation

    Axon directionality on 1400 nm topographical pitch surface or 0 nm pitch flat surface is not altered by diffuse soluble factors. DRG were plated onto either 0 nm pitch control surfaces or 1400 nm pitch surfaces with a basal media enriched with either NGF, GDNF, BDNF or NT-3. Six days after plating, DRG were fixed, stained and imaged using confocal microscopy. The directionality of axons 1500 µm from the DRG body was quantified. DRG cultured on the 1400 nm pitch topography had 85–100% of their axons parallel to the underlying surface topography, independent of the soluble growth factor that was added to the media. The DRG cultured on the 0 nm pitch control substrates had individual axons that ranged from 0–100% parallel to an arbitrary, consistent axis. Each 1400 nm pitch category was significantly different (** = p

    Journal: Biomolecules

    Article Title: Submicron Topographically Patterned 3D Substrates Enhance Directional Axon Outgrowth of Dorsal Root Ganglia Cultured Ex Vivo

    doi: 10.3390/biom12081059

    Figure Lengend Snippet: Axon directionality on 1400 nm topographical pitch surface or 0 nm pitch flat surface is not altered by diffuse soluble factors. DRG were plated onto either 0 nm pitch control surfaces or 1400 nm pitch surfaces with a basal media enriched with either NGF, GDNF, BDNF or NT-3. Six days after plating, DRG were fixed, stained and imaged using confocal microscopy. The directionality of axons 1500 µm from the DRG body was quantified. DRG cultured on the 1400 nm pitch topography had 85–100% of their axons parallel to the underlying surface topography, independent of the soluble growth factor that was added to the media. The DRG cultured on the 0 nm pitch control substrates had individual axons that ranged from 0–100% parallel to an arbitrary, consistent axis. Each 1400 nm pitch category was significantly different (** = p

    Article Snippet: DRGs were maintained in serum-free media containing 5 ng/mL of nerve growth factor (NGF) (Alomone Labs, Ltd., Jerusalem, Israel) and incubated at 37 °C with 5% CO2 for up to 6 days.

    Techniques: Staining, Confocal Microscopy, Cell Culture

    NGF modulates the expression of the main proteins involved in cholesterol homeostasis in U373 cells and enhances ApoE secretion in U373 culture medium. ( A – G ) Representative Western blot and densitometric analysis of SREBP-1, SREBP-2, HMGCR, LDLr, NPC1, ABCA1, and ApoE in U373 cells treated with vehicle (Ctrl) and NGF (100 ng/mL) for 48 h. n = 6 different experiments. Actin and vinculin were used as loading controls. ( H ) Quantification of ApoE levels (µg/mL) by ELISA assay in vehicle- and NGF-treated U373 culture medium. n = 3 different experiments. Data represent means ± SD. Statistical analysis was assessed by using unpaired Student’s t test. * p

    Journal: International Journal of Molecular Sciences

    Article Title: NGF Modulates Cholesterol Metabolism and Stimulates ApoE Secretion in Glial Cells Conferring Neuroprotection against Oxidative Stress

    doi: 10.3390/ijms23094842

    Figure Lengend Snippet: NGF modulates the expression of the main proteins involved in cholesterol homeostasis in U373 cells and enhances ApoE secretion in U373 culture medium. ( A – G ) Representative Western blot and densitometric analysis of SREBP-1, SREBP-2, HMGCR, LDLr, NPC1, ABCA1, and ApoE in U373 cells treated with vehicle (Ctrl) and NGF (100 ng/mL) for 48 h. n = 6 different experiments. Actin and vinculin were used as loading controls. ( H ) Quantification of ApoE levels (µg/mL) by ELISA assay in vehicle- and NGF-treated U373 culture medium. n = 3 different experiments. Data represent means ± SD. Statistical analysis was assessed by using unpaired Student’s t test. * p

    Article Snippet: U373 cells were treated with NGF (Alomone Labs, Jerusalem, Israel, N-245) at the dose of 100 ng/mL for 48 h in all the experiments.

    Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    Effect of U373 conditioned medium on neuronal differentiation. ( A ) Representative images in bright field of N1E-115 cells cultured in fresh DMEM (Ctrl), in conditioned medium derived from control U373 (U373-Ctrl), NGF-pre-treated U373 (U373-NGF), and NGF-pre-treated U373 silenced for ApoE (U373-NGF ApoE siRNA) for 48 h. ( B ) Morphological analysis of neurite-bearing cells, neurite length and number of neurites of N1E-115 cells in the presence of fresh DMEM (Ctrl), in conditioned medium derived from control U373 (U373-Ctrl), NGF-pre-treated U373 (U373-NGF), and NGF-pre-treated U373 silenced for ApoE (U373-NGF ApoE siRNA) for 48 h. n = 5 different experiments. Data represent means ± SD. Statistical analysis was performed by using one-way ANOVA, followed by Tukey’s post hoc test.

    Journal: International Journal of Molecular Sciences

    Article Title: NGF Modulates Cholesterol Metabolism and Stimulates ApoE Secretion in Glial Cells Conferring Neuroprotection against Oxidative Stress

    doi: 10.3390/ijms23094842

    Figure Lengend Snippet: Effect of U373 conditioned medium on neuronal differentiation. ( A ) Representative images in bright field of N1E-115 cells cultured in fresh DMEM (Ctrl), in conditioned medium derived from control U373 (U373-Ctrl), NGF-pre-treated U373 (U373-NGF), and NGF-pre-treated U373 silenced for ApoE (U373-NGF ApoE siRNA) for 48 h. ( B ) Morphological analysis of neurite-bearing cells, neurite length and number of neurites of N1E-115 cells in the presence of fresh DMEM (Ctrl), in conditioned medium derived from control U373 (U373-Ctrl), NGF-pre-treated U373 (U373-NGF), and NGF-pre-treated U373 silenced for ApoE (U373-NGF ApoE siRNA) for 48 h. n = 5 different experiments. Data represent means ± SD. Statistical analysis was performed by using one-way ANOVA, followed by Tukey’s post hoc test.

    Article Snippet: U373 cells were treated with NGF (Alomone Labs, Jerusalem, Israel, N-245) at the dose of 100 ng/mL for 48 h in all the experiments.

    Techniques: Cell Culture, Derivative Assay

    Effects of U373 conditioned medium on oxidative stress in neurons. Representative images in bright field and quantitative assessment of neuronal morphology of N1E-115. Cells were previously treated (+) or not (−) with rotenone (0.1 µM) for 16 h, and then cultured in fresh DMEM (Ctrl), in conditioned medium derived from control U373 (U373-Ctrl), NGF-pre-treated U373 (U373-NGF), and NGF-pre-treated U373 silenced for apoE (U373-NGF ApoE siRNA) for 48 h. n = 5 different experiments. Data represent means ± SD. Statistical analysis was assessed by using one-way ANOVA, followed by Tukey’s post hoc test. ** p

    Journal: International Journal of Molecular Sciences

    Article Title: NGF Modulates Cholesterol Metabolism and Stimulates ApoE Secretion in Glial Cells Conferring Neuroprotection against Oxidative Stress

    doi: 10.3390/ijms23094842

    Figure Lengend Snippet: Effects of U373 conditioned medium on oxidative stress in neurons. Representative images in bright field and quantitative assessment of neuronal morphology of N1E-115. Cells were previously treated (+) or not (−) with rotenone (0.1 µM) for 16 h, and then cultured in fresh DMEM (Ctrl), in conditioned medium derived from control U373 (U373-Ctrl), NGF-pre-treated U373 (U373-NGF), and NGF-pre-treated U373 silenced for apoE (U373-NGF ApoE siRNA) for 48 h. n = 5 different experiments. Data represent means ± SD. Statistical analysis was assessed by using one-way ANOVA, followed by Tukey’s post hoc test. ** p

    Article Snippet: U373 cells were treated with NGF (Alomone Labs, Jerusalem, Israel, N-245) at the dose of 100 ng/mL for 48 h in all the experiments.

    Techniques: Cell Culture, Derivative Assay

    NGF effects on neutral lipids and cholesterol content. ( A ) U373 cells were treated with vehicle (Ctrl) or NGF (100 ng/mL) for 48 h and then stained with Oil Red O to visualize the intracellular content of neutral lipids. n = 3 different experiments. ( B ) Representative image of filipin staining performed on U373 cells treated with vehicle (Ctrl) and NGF (100 ng/mL) for 48 h. n = 3 different experiments. ( C ) Quantification of intracellular cholesterol levels (total cholesterol, free cholesterol, and cholesteryl esters) in vehicle- and NGF-treated U373 cells. n = 3 different experiments. ( D ) Cholesterol quantification (total cholesterol, free cholesterol, and cholesteryl esters) in the culture medium of U373 cells treated with vehicle (Ctrl) and NGF (100 ng/mL) for 48 h. n = 3 different experiments. Data represent means ± SD. Statistical analysis was performed by using unpaired Student’s t -test. ** p

    Journal: International Journal of Molecular Sciences

    Article Title: NGF Modulates Cholesterol Metabolism and Stimulates ApoE Secretion in Glial Cells Conferring Neuroprotection against Oxidative Stress

    doi: 10.3390/ijms23094842

    Figure Lengend Snippet: NGF effects on neutral lipids and cholesterol content. ( A ) U373 cells were treated with vehicle (Ctrl) or NGF (100 ng/mL) for 48 h and then stained with Oil Red O to visualize the intracellular content of neutral lipids. n = 3 different experiments. ( B ) Representative image of filipin staining performed on U373 cells treated with vehicle (Ctrl) and NGF (100 ng/mL) for 48 h. n = 3 different experiments. ( C ) Quantification of intracellular cholesterol levels (total cholesterol, free cholesterol, and cholesteryl esters) in vehicle- and NGF-treated U373 cells. n = 3 different experiments. ( D ) Cholesterol quantification (total cholesterol, free cholesterol, and cholesteryl esters) in the culture medium of U373 cells treated with vehicle (Ctrl) and NGF (100 ng/mL) for 48 h. n = 3 different experiments. Data represent means ± SD. Statistical analysis was performed by using unpaired Student’s t -test. ** p

    Article Snippet: U373 cells were treated with NGF (Alomone Labs, Jerusalem, Israel, N-245) at the dose of 100 ng/mL for 48 h in all the experiments.

    Techniques: Staining

    ApoE secretion by NGF-treated U373 cells provides neuroprotection from oxidative stress. ( A ) Representative scheme of astrocyte–neuron co-cultures set up as described in the Materials and Methods Section. Neurite-bearing cells, neurite length, and cell number were assessed 48 h after the establishment of co-culture. ( B ) Representative images in bright field and quantitative assessment of neuronal morphology of N1E-115 cells, previously treated (+) or not (−) with rotenone (0.1 µM) for 16 h. N1E-115 were then kept in fresh DMEM (Ctrl), or co-cultured with control U373 (U373-Ctrl), NGF-pre-treated U373 (U373-NGF), and NGF-pre-treated U373 silenced for ApoE (U373-NGF ApoE siRNA) for 48 h. n = 5 different experiments. Data represent means ± SD. Statistical analysis was carried out by using one-way ANOVA, followed by Tukey’s post hoc test. * p

    Journal: International Journal of Molecular Sciences

    Article Title: NGF Modulates Cholesterol Metabolism and Stimulates ApoE Secretion in Glial Cells Conferring Neuroprotection against Oxidative Stress

    doi: 10.3390/ijms23094842

    Figure Lengend Snippet: ApoE secretion by NGF-treated U373 cells provides neuroprotection from oxidative stress. ( A ) Representative scheme of astrocyte–neuron co-cultures set up as described in the Materials and Methods Section. Neurite-bearing cells, neurite length, and cell number were assessed 48 h after the establishment of co-culture. ( B ) Representative images in bright field and quantitative assessment of neuronal morphology of N1E-115 cells, previously treated (+) or not (−) with rotenone (0.1 µM) for 16 h. N1E-115 were then kept in fresh DMEM (Ctrl), or co-cultured with control U373 (U373-Ctrl), NGF-pre-treated U373 (U373-NGF), and NGF-pre-treated U373 silenced for ApoE (U373-NGF ApoE siRNA) for 48 h. n = 5 different experiments. Data represent means ± SD. Statistical analysis was carried out by using one-way ANOVA, followed by Tukey’s post hoc test. * p

    Article Snippet: U373 cells were treated with NGF (Alomone Labs, Jerusalem, Israel, N-245) at the dose of 100 ng/mL for 48 h in all the experiments.

    Techniques: Co-Culture Assay, Cell Culture