ngf  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs ngf
    Hypothesis of inhibition mechanism of the <t>NGF-induced</t> neurite extension by chronic irradiation with low-dose-rate 137 <t>Cs-γ</t> rays via Ca 2+ /calmodulin-dependent kinase II activation. Chronic irradiation with low-dose-rate γ rays activates CaMKII in the downstream of the Ca-dependent signal induced by NGF. The production of reactive oxygen species generated by irradiation may be involved in this activation of CaMKII. The CaMK II suppresses NGF-induced neuronal extension by inhibition of the activation of Rac1 downstream of the PI3K-Akt signal. IQGAP1, which is considered to contribute to neurite extension, may be involved in this inhibition of Rac1 activity. NGF = nerve growth factor, ERK = extracellular signal–regulated kinase, PI3K = phosphoinositide 3-kinase, IP3 = inositol trisphosphate, CaM = calmodulin, CaMKII = Ca 2+ /calmodulin-dependent protein kinase II, ROS = reactive oxygen species, IQGAP1 = Ras GTPase-activating-like protein.
    Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngf/product/Alomone Labs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ngf - by Bioz Stars, 2022-11
    94/100 stars

    Images

    1) Product Images from "Chronic irradiation with low-dose-rate 137Cs-γ rays inhibits NGF-induced neurite extension of PC12 cells via Ca2+/calmodulin-dependent kinase II activation"

    Article Title: Chronic irradiation with low-dose-rate 137Cs-γ rays inhibits NGF-induced neurite extension of PC12 cells via Ca2+/calmodulin-dependent kinase II activation

    Journal: Journal of Radiation Research

    doi: 10.1093/jrr/rrx032

    Hypothesis of inhibition mechanism of the NGF-induced neurite extension by chronic irradiation with low-dose-rate 137 Cs-γ rays via Ca 2+ /calmodulin-dependent kinase II activation. Chronic irradiation with low-dose-rate γ rays activates CaMKII in the downstream of the Ca-dependent signal induced by NGF. The production of reactive oxygen species generated by irradiation may be involved in this activation of CaMKII. The CaMK II suppresses NGF-induced neuronal extension by inhibition of the activation of Rac1 downstream of the PI3K-Akt signal. IQGAP1, which is considered to contribute to neurite extension, may be involved in this inhibition of Rac1 activity. NGF = nerve growth factor, ERK = extracellular signal–regulated kinase, PI3K = phosphoinositide 3-kinase, IP3 = inositol trisphosphate, CaM = calmodulin, CaMKII = Ca 2+ /calmodulin-dependent protein kinase II, ROS = reactive oxygen species, IQGAP1 = Ras GTPase-activating-like protein.
    Figure Legend Snippet: Hypothesis of inhibition mechanism of the NGF-induced neurite extension by chronic irradiation with low-dose-rate 137 Cs-γ rays via Ca 2+ /calmodulin-dependent kinase II activation. Chronic irradiation with low-dose-rate γ rays activates CaMKII in the downstream of the Ca-dependent signal induced by NGF. The production of reactive oxygen species generated by irradiation may be involved in this activation of CaMKII. The CaMK II suppresses NGF-induced neuronal extension by inhibition of the activation of Rac1 downstream of the PI3K-Akt signal. IQGAP1, which is considered to contribute to neurite extension, may be involved in this inhibition of Rac1 activity. NGF = nerve growth factor, ERK = extracellular signal–regulated kinase, PI3K = phosphoinositide 3-kinase, IP3 = inositol trisphosphate, CaM = calmodulin, CaMKII = Ca 2+ /calmodulin-dependent protein kinase II, ROS = reactive oxygen species, IQGAP1 = Ras GTPase-activating-like protein.

    Techniques Used: Inhibition, Irradiation, Activation Assay, Generated, Activity Assay, Chick Chorioallantoic Membrane Assay

    Irradiation with 137 Cs γ-rays depresses NGF-induced neurite extension in PC12 cells. (a) Phase-contrast micrographs, (b) lengths of neurites and (c) numbers of neurites in PC12 cells after 5 days of NGF stimulation or non-stimulation, with or without 137 Csγ-ray irradiation. Lengths or numbers of neurites are expressed as the relative ratio to the non-irradiated group. Data are presented as the mean ± standard error of triplicate samples. * P
    Figure Legend Snippet: Irradiation with 137 Cs γ-rays depresses NGF-induced neurite extension in PC12 cells. (a) Phase-contrast micrographs, (b) lengths of neurites and (c) numbers of neurites in PC12 cells after 5 days of NGF stimulation or non-stimulation, with or without 137 Csγ-ray irradiation. Lengths or numbers of neurites are expressed as the relative ratio to the non-irradiated group. Data are presented as the mean ± standard error of triplicate samples. * P

    Techniques Used: Irradiation

    Western blot analysis of NGF stimulation–related proteins in PC12 cells. Immunoblot showing varying levels of (a) phosphorylated NGF receptor (P-NGFR, upper) and NGF receptor (NGFR, lower), (b) phosphorylated ERK (P-ERK, upper) and ERK (lower), (c) tyrosine hydroxylase (TH) observed in PC12 cells after 5 days of NGF stimulation or non-stimulation, with or without 137 Csγ-ray irradiation. Similar results were obtained in three separate experiments. NGF = nerve growth factor, ERK = extracellular signal–regulated kinase.
    Figure Legend Snippet: Western blot analysis of NGF stimulation–related proteins in PC12 cells. Immunoblot showing varying levels of (a) phosphorylated NGF receptor (P-NGFR, upper) and NGF receptor (NGFR, lower), (b) phosphorylated ERK (P-ERK, upper) and ERK (lower), (c) tyrosine hydroxylase (TH) observed in PC12 cells after 5 days of NGF stimulation or non-stimulation, with or without 137 Csγ-ray irradiation. Similar results were obtained in three separate experiments. NGF = nerve growth factor, ERK = extracellular signal–regulated kinase.

    Techniques Used: Western Blot, Irradiation

    Inhibition of CaMKII activity results in 137 Csγ-ray irradiation-induced depression of NGF-induced neurite extension in PC12 cells. (a) Immunoblot showing varying levels of phosphorylated CaMKII (P-CaMKII, upper) and CaMKII (lower) observed in PC12 cells after 5 days of NGF stimulation or non-stimulation, with or without 137 Csγ-ray irradiation in the presence or absence of KN-62. Similar results were obtained in three separate experiments. (b) Lengths and (c) numbers of neurites in PC12 cells after 5 days of NGF stimulation or non-stimulation, with or without 137 Csγ-ray irradiation in the presence or absence of KN-62. Lengths or numbers of neurites are expressed as the relative ratio to the non-irradiated group. Data are presented as the mean ± standard error of triplicate samples. * P
    Figure Legend Snippet: Inhibition of CaMKII activity results in 137 Csγ-ray irradiation-induced depression of NGF-induced neurite extension in PC12 cells. (a) Immunoblot showing varying levels of phosphorylated CaMKII (P-CaMKII, upper) and CaMKII (lower) observed in PC12 cells after 5 days of NGF stimulation or non-stimulation, with or without 137 Csγ-ray irradiation in the presence or absence of KN-62. Similar results were obtained in three separate experiments. (b) Lengths and (c) numbers of neurites in PC12 cells after 5 days of NGF stimulation or non-stimulation, with or without 137 Csγ-ray irradiation in the presence or absence of KN-62. Lengths or numbers of neurites are expressed as the relative ratio to the non-irradiated group. Data are presented as the mean ± standard error of triplicate samples. * P

    Techniques Used: Inhibition, Activity Assay, Irradiation

    Irradiation with 137 Cs γ-rays attenuates NGF-induced Rac1 activation without increasing phosphorylation of Akt in PC12 cells. (a) The activity of Rac1 in PC12 cells after 5 days of NGF stimulation or non-stimulation, with or without 137 Csγ-ray irradiation. Rac1 activity is expressed as the relative ratio to the NGF non-stimulated group without irradiation. Data are presented as the mean ± standard error of triplicate samples. ** P
    Figure Legend Snippet: Irradiation with 137 Cs γ-rays attenuates NGF-induced Rac1 activation without increasing phosphorylation of Akt in PC12 cells. (a) The activity of Rac1 in PC12 cells after 5 days of NGF stimulation or non-stimulation, with or without 137 Csγ-ray irradiation. Rac1 activity is expressed as the relative ratio to the NGF non-stimulated group without irradiation. Data are presented as the mean ± standard error of triplicate samples. ** P

    Techniques Used: Irradiation, Activation Assay, Activity Assay

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Alomone Labs 7s mouse ngf
    7s Mouse Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7s mouse ngf/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    7s mouse ngf - by Bioz Stars, 2022-11
    94/100 stars
      Buy from Supplier

    Image Search Results