gdna  (New England Biolabs)


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    Name:
    Monarch Genomic DNA Purification Kit
    Description:
    The Monarch Genomic DNA Purification Kit is a comprehensive solution for cell lysis RNA removal and purification of intact genomic DNA gDNA from a wide variety of biological samples including cultured cells blood and mammalian tissues Additionally bacteria and yeast can be processed with extra steps to enhance lysis in these tough to lyse samples Protocols are also included to enable purification from clinically relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA Purified gDNA has high quality metrics including A260 A280 1 8 and A260 A230 2 0 high DIN scores and minimal residual RNA The purified gDNA is suitable for downstream applications such as end point PCR qPCR and library prep for NGS It typically has a peak size of 50kb making this kit an excellent choice upstream of long read sequencing platforms
    Catalog Number:
    T3010L
    Price:
    395
    Category:
    Genomic DNA Purification Kits
    Size:
    150 preps
    Buy from Supplier


    Structured Review

    New England Biolabs gdna
    Monarch Genomic DNA Purification Kit
    The Monarch Genomic DNA Purification Kit is a comprehensive solution for cell lysis RNA removal and purification of intact genomic DNA gDNA from a wide variety of biological samples including cultured cells blood and mammalian tissues Additionally bacteria and yeast can be processed with extra steps to enhance lysis in these tough to lyse samples Protocols are also included to enable purification from clinically relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA Purified gDNA has high quality metrics including A260 A280 1 8 and A260 A230 2 0 high DIN scores and minimal residual RNA The purified gDNA is suitable for downstream applications such as end point PCR qPCR and library prep for NGS It typically has a peak size of 50kb making this kit an excellent choice upstream of long read sequencing platforms
    https://www.bioz.com/result/gdna/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gdna - by Bioz Stars, 2021-05
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics
    Article Snippet: Briefly, for unmarked deletions, ∼1kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector , . .. Fragments amplified from P. aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Monarch PCR Purification kit (New England Biolabs) were used for Gibson assembly together with pMQ30 cut with SacI and HindIII. .. The assembled construct was then transformed into Escherichia coli DH10B, with transformants being selected in LB with 20 µg/mL gentamicin.

    Article Title: A High-Efficacy CRISPR Interference System for Gene Function Discovery in Zymomonas mobilis
    Article Snippet: Oligonucleotides (2 μM each) were annealed in 1× CutSmart buffer (NEB) at 95°C for 5 min, followed by cooling to room temperature. .. For method two, fragments were amplified by PCR with primers oJMP197 and oJMP198 from a 78-nt oligonucleotide, followed by digestion with BsaI-HF-v2 (catalog number R3733; NEB) and purification with the Monarch DNA purification kit (NEB) following the manufacturer’s oligonucleotide purification protocol. .. Inserts (2 μl of a 1:40 dilution of annealed oligonucleotides or 2 ng purified digested PCR product) were ligated into 50 ng BsaI-digested vector.

    Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics
    Article Snippet: Briefly, for unmarked deletions, approximately 1-kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector [ , ]. .. Fragments amplified from P . aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Monarch PCR Purification kit (New England Biolabs, Ipswich, Massachusetts, USA) were used for Gibson assembly together with pMQ30 cut with SacI and HindIII. .. The assembled construct was then transformed into E . coli DH10B, with transformants being selected in LB with 20 μg/mL gentamicin.

    Polymerase Chain Reaction:

    Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics
    Article Snippet: Briefly, for unmarked deletions, ∼1kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector , . .. Fragments amplified from P. aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Monarch PCR Purification kit (New England Biolabs) were used for Gibson assembly together with pMQ30 cut with SacI and HindIII. .. The assembled construct was then transformed into Escherichia coli DH10B, with transformants being selected in LB with 20 µg/mL gentamicin.

    Article Title: A High-Efficacy CRISPR Interference System for Gene Function Discovery in Zymomonas mobilis
    Article Snippet: Oligonucleotides (2 μM each) were annealed in 1× CutSmart buffer (NEB) at 95°C for 5 min, followed by cooling to room temperature. .. For method two, fragments were amplified by PCR with primers oJMP197 and oJMP198 from a 78-nt oligonucleotide, followed by digestion with BsaI-HF-v2 (catalog number R3733; NEB) and purification with the Monarch DNA purification kit (NEB) following the manufacturer’s oligonucleotide purification protocol. .. Inserts (2 μl of a 1:40 dilution of annealed oligonucleotides or 2 ng purified digested PCR product) were ligated into 50 ng BsaI-digested vector.

    Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics
    Article Snippet: Briefly, for unmarked deletions, approximately 1-kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector [ , ]. .. Fragments amplified from P . aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Monarch PCR Purification kit (New England Biolabs, Ipswich, Massachusetts, USA) were used for Gibson assembly together with pMQ30 cut with SacI and HindIII. .. The assembled construct was then transformed into E . coli DH10B, with transformants being selected in LB with 20 μg/mL gentamicin.

    Purification:

    Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics
    Article Snippet: Briefly, for unmarked deletions, ∼1kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector , . .. Fragments amplified from P. aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Monarch PCR Purification kit (New England Biolabs) were used for Gibson assembly together with pMQ30 cut with SacI and HindIII. .. The assembled construct was then transformed into Escherichia coli DH10B, with transformants being selected in LB with 20 µg/mL gentamicin.

    Article Title: A High-Efficacy CRISPR Interference System for Gene Function Discovery in Zymomonas mobilis
    Article Snippet: Oligonucleotides (2 μM each) were annealed in 1× CutSmart buffer (NEB) at 95°C for 5 min, followed by cooling to room temperature. .. For method two, fragments were amplified by PCR with primers oJMP197 and oJMP198 from a 78-nt oligonucleotide, followed by digestion with BsaI-HF-v2 (catalog number R3733; NEB) and purification with the Monarch DNA purification kit (NEB) following the manufacturer’s oligonucleotide purification protocol. .. Inserts (2 μl of a 1:40 dilution of annealed oligonucleotides or 2 ng purified digested PCR product) were ligated into 50 ng BsaI-digested vector.

    Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics
    Article Snippet: Briefly, for unmarked deletions, approximately 1-kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector [ , ]. .. Fragments amplified from P . aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Monarch PCR Purification kit (New England Biolabs, Ipswich, Massachusetts, USA) were used for Gibson assembly together with pMQ30 cut with SacI and HindIII. .. The assembled construct was then transformed into E . coli DH10B, with transformants being selected in LB with 20 μg/mL gentamicin.

    Infection:

    Article Title: MicroRNA-21 depletion by CRISPR/Cas9 in CNE2 nasopharyngeal cells hinders proliferation and induces apoptosis by targeting the PI3K/AKT/MOTOR signaling pathway
    Article Snippet: The oligonucleotide sequence was synthesized by Shanghai Genechem Co., LTD., and each pair of oligonucleotides was transformed into DS-DNA fragments through annealing and then linked onto the Cas9-Easy-lentivirus vector. .. The CNE2 cells were collected after 72 h of infection, along with the application of mammalian genomic DNA extraction kit to extract genome DNA and T7EN1 (NEB, USA; M0302S) to detect the editing efficiency of gRNAs. .. The whole genomic DNA was extracted by utilizing the DNA Extraction kit (Beyotime, Shaihai, China) reported previously [ ].

    DNA Extraction:

    Article Title: MicroRNA-21 depletion by CRISPR/Cas9 in CNE2 nasopharyngeal cells hinders proliferation and induces apoptosis by targeting the PI3K/AKT/MOTOR signaling pathway
    Article Snippet: The oligonucleotide sequence was synthesized by Shanghai Genechem Co., LTD., and each pair of oligonucleotides was transformed into DS-DNA fragments through annealing and then linked onto the Cas9-Easy-lentivirus vector. .. The CNE2 cells were collected after 72 h of infection, along with the application of mammalian genomic DNA extraction kit to extract genome DNA and T7EN1 (NEB, USA; M0302S) to detect the editing efficiency of gRNAs. .. The whole genomic DNA was extracted by utilizing the DNA Extraction kit (Beyotime, Shaihai, China) reported previously [ ].

    Mutagenesis:

    Article Title: The Borrelia burgdorferi Glycosaminoglycan Binding Protein Bgp in the B31 Strain Is Not Essential for Infectivity despite Facilitating Adherence and Tissue Colonization
    Article Snippet: .. Genomic DNA from the bgp mutant strain was isolated by standard genomic DNA purification, digested with the restriction enzyme Spe1 (New England BioLabs, Ipswich, MA), and cloned in pUC19 (Thermo Scientific, Waltham, MA). .. Transformation of Top10 competent E. coli cells was followed by selection on gentamicin (15 μg/ml)-containing Luria-Bertani plates to select for the DNA fragment containing the resistance cassette of the transposon.

    Isolation:

    Article Title: The Borrelia burgdorferi Glycosaminoglycan Binding Protein Bgp in the B31 Strain Is Not Essential for Infectivity despite Facilitating Adherence and Tissue Colonization
    Article Snippet: .. Genomic DNA from the bgp mutant strain was isolated by standard genomic DNA purification, digested with the restriction enzyme Spe1 (New England BioLabs, Ipswich, MA), and cloned in pUC19 (Thermo Scientific, Waltham, MA). .. Transformation of Top10 competent E. coli cells was followed by selection on gentamicin (15 μg/ml)-containing Luria-Bertani plates to select for the DNA fragment containing the resistance cassette of the transposon.

    Article Title: Rapid Identification of Methylase Specificity (RIMS-seq) jointly identifies methylated motifs and generates shotgun sequencing of bacterial genomes
    Article Snippet: E. coli K12 MG1655 genomic DNA was extracted from a cell culture using the DNEasy Blood and Tissue kit (69504, Qiagen). .. All the other gDNA from the bacteria presented in were isolated using the Monarch genomic DNA purification kit (T3010S, New England Biolabs). .. Xp12 phage genomic DNA was obtained from Peter Weigele and Yian-Jiun Lee at New England Biolabs.

    DNA Purification:

    Article Title: The Borrelia burgdorferi Glycosaminoglycan Binding Protein Bgp in the B31 Strain Is Not Essential for Infectivity despite Facilitating Adherence and Tissue Colonization
    Article Snippet: .. Genomic DNA from the bgp mutant strain was isolated by standard genomic DNA purification, digested with the restriction enzyme Spe1 (New England BioLabs, Ipswich, MA), and cloned in pUC19 (Thermo Scientific, Waltham, MA). .. Transformation of Top10 competent E. coli cells was followed by selection on gentamicin (15 μg/ml)-containing Luria-Bertani plates to select for the DNA fragment containing the resistance cassette of the transposon.

    Article Title: A High-Efficacy CRISPR Interference System for Gene Function Discovery in Zymomonas mobilis
    Article Snippet: Oligonucleotides (2 μM each) were annealed in 1× CutSmart buffer (NEB) at 95°C for 5 min, followed by cooling to room temperature. .. For method two, fragments were amplified by PCR with primers oJMP197 and oJMP198 from a 78-nt oligonucleotide, followed by digestion with BsaI-HF-v2 (catalog number R3733; NEB) and purification with the Monarch DNA purification kit (NEB) following the manufacturer’s oligonucleotide purification protocol. .. Inserts (2 μl of a 1:40 dilution of annealed oligonucleotides or 2 ng purified digested PCR product) were ligated into 50 ng BsaI-digested vector.

    Article Title: A New Protocol for Targeted insertion using CRISPR-Cas9, Oligo Single-Stranded DNA and Protoplast Regeneration
    Article Snippet: .. Whole genome sequencing for off-targeted insertion analysis Leaves of N. benthamiana protoplast regenerants were collected for Genomic DNA purification. .. Genomic DNA for genome sequencing was extracted using a Plant DNA Purification Kit (DP320, Tiangen, http://www.tiangen.com/en/ ).

    Article Title: Rapid Identification of Methylase Specificity (RIMS-seq) jointly identifies methylated motifs and generates shotgun sequencing of bacterial genomes
    Article Snippet: E. coli K12 MG1655 genomic DNA was extracted from a cell culture using the DNEasy Blood and Tissue kit (69504, Qiagen). .. All the other gDNA from the bacteria presented in were isolated using the Monarch genomic DNA purification kit (T3010S, New England Biolabs). .. Xp12 phage genomic DNA was obtained from Peter Weigele and Yian-Jiun Lee at New England Biolabs.

    Clone Assay:

    Article Title: The Borrelia burgdorferi Glycosaminoglycan Binding Protein Bgp in the B31 Strain Is Not Essential for Infectivity despite Facilitating Adherence and Tissue Colonization
    Article Snippet: .. Genomic DNA from the bgp mutant strain was isolated by standard genomic DNA purification, digested with the restriction enzyme Spe1 (New England BioLabs, Ipswich, MA), and cloned in pUC19 (Thermo Scientific, Waltham, MA). .. Transformation of Top10 competent E. coli cells was followed by selection on gentamicin (15 μg/ml)-containing Luria-Bertani plates to select for the DNA fragment containing the resistance cassette of the transposon.

    Sequencing:

    Article Title: A New Protocol for Targeted insertion using CRISPR-Cas9, Oligo Single-Stranded DNA and Protoplast Regeneration
    Article Snippet: .. Whole genome sequencing for off-targeted insertion analysis Leaves of N. benthamiana protoplast regenerants were collected for Genomic DNA purification. .. Genomic DNA for genome sequencing was extracted using a Plant DNA Purification Kit (DP320, Tiangen, http://www.tiangen.com/en/ ).

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