gdna (New England Biolabs)
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Name:
Monarch Genomic DNA Purification Kit
Description:
The Monarch Genomic DNA Purification Kit is a comprehensive solution for cell lysis RNA removal and purification of intact genomic DNA gDNA from a wide variety of biological samples including cultured cells blood and mammalian tissues Additionally bacteria and yeast can be processed with extra steps to enhance lysis in these tough to lyse samples Protocols are also included to enable purification from clinically relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA Purified gDNA has high quality metrics including A260 A280 1 8 and A260 A230 2 0 high DIN scores and minimal residual RNA The purified gDNA is suitable for downstream applications such as end point PCR qPCR and library prep for NGS It typically has a peak size of 50kb making this kit an excellent choice upstream of long read sequencing platforms
Catalog Number:
T3010L
Price:
395
Category:
Genomic DNA Purification Kits
Size:
150 preps
|
Buy from Supplier |
Structured Review
New England Biolabs
gdna
The Monarch Genomic DNA Purification Kit is a comprehensive solution for cell lysis RNA removal and purification of intact genomic DNA gDNA from a wide variety of biological samples including cultured cells blood and mammalian tissues Additionally bacteria and yeast can be processed with extra steps to enhance lysis in these tough to lyse samples Protocols are also included to enable purification from clinically relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA Purified gDNA has high quality metrics including A260 A280 1 8 and A260 A230 2 0 high DIN scores and minimal residual RNA The purified gDNA is suitable for downstream applications such as end point PCR qPCR and library prep for NGS It typically has a peak size of 50kb making this kit an excellent choice upstream of long read sequencing platforms
https://www.bioz.com/result/gdna/product/New England Biolabs
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
The Monarch Genomic DNA Purification Kit is a comprehensive solution for cell lysis RNA removal and purification of intact genomic DNA gDNA from a wide variety of biological samples including cultured cells blood and mammalian tissues Additionally bacteria and yeast can be processed with extra steps to enhance lysis in these tough to lyse samples Protocols are also included to enable purification from clinically relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA Purified gDNA has high quality metrics including A260 A280 1 8 and A260 A230 2 0 high DIN scores and minimal residual RNA The purified gDNA is suitable for downstream applications such as end point PCR qPCR and library prep for NGS It typically has a peak size of 50kb making this kit an excellent choice upstream of long read sequencing platforms
https://www.bioz.com/result/gdna/product/New England Biolabs
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
gdna - by Bioz Stars,
2021-05
99/100 stars
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Related Articles
Amplification:Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics Article Snippet: Briefly, for unmarked deletions, ∼1kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector , . .. Fragments amplified from P. aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Article Title: A High-Efficacy CRISPR Interference System for Gene Function Discovery in Zymomonas mobilis Article Snippet: Oligonucleotides (2 μM each) were annealed in 1× CutSmart buffer (NEB) at 95°C for 5 min, followed by cooling to room temperature. .. For method two, fragments were amplified by PCR with primers oJMP197 and oJMP198 from a 78-nt oligonucleotide, followed by digestion with BsaI-HF-v2 (catalog number R3733; NEB) and purification with the Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics Article Snippet: Briefly, for unmarked deletions, approximately 1-kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector [ , ]. .. Fragments amplified from P . aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Polymerase Chain Reaction:Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics Article Snippet: Briefly, for unmarked deletions, ∼1kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector , . .. Fragments amplified from P. aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Article Title: A High-Efficacy CRISPR Interference System for Gene Function Discovery in Zymomonas mobilis Article Snippet: Oligonucleotides (2 μM each) were annealed in 1× CutSmart buffer (NEB) at 95°C for 5 min, followed by cooling to room temperature. .. For method two, fragments were amplified by PCR with primers oJMP197 and oJMP198 from a 78-nt oligonucleotide, followed by digestion with BsaI-HF-v2 (catalog number R3733; NEB) and purification with the Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics Article Snippet: Briefly, for unmarked deletions, approximately 1-kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector [ , ]. .. Fragments amplified from P . aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Purification:Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics Article Snippet: Briefly, for unmarked deletions, ∼1kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector , . .. Fragments amplified from P. aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Article Title: A High-Efficacy CRISPR Interference System for Gene Function Discovery in Zymomonas mobilis Article Snippet: Oligonucleotides (2 μM each) were annealed in 1× CutSmart buffer (NEB) at 95°C for 5 min, followed by cooling to room temperature. .. For method two, fragments were amplified by PCR with primers oJMP197 and oJMP198 from a 78-nt oligonucleotide, followed by digestion with BsaI-HF-v2 (catalog number R3733; NEB) and purification with the Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics Article Snippet: Briefly, for unmarked deletions, approximately 1-kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector [ , ]. .. Fragments amplified from P . aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Infection:Article Title: MicroRNA-21 depletion by CRISPR/Cas9 in CNE2 nasopharyngeal cells hinders proliferation and induces apoptosis by targeting the PI3K/AKT/MOTOR signaling pathway Article Snippet: The oligonucleotide sequence was synthesized by Shanghai Genechem Co., LTD., and each pair of oligonucleotides was transformed into DS-DNA fragments through annealing and then linked onto the Cas9-Easy-lentivirus vector. .. The CNE2 cells were collected after 72 h of infection, along with the application of DNA Extraction:Article Title: MicroRNA-21 depletion by CRISPR/Cas9 in CNE2 nasopharyngeal cells hinders proliferation and induces apoptosis by targeting the PI3K/AKT/MOTOR signaling pathway Article Snippet: The oligonucleotide sequence was synthesized by Shanghai Genechem Co., LTD., and each pair of oligonucleotides was transformed into DS-DNA fragments through annealing and then linked onto the Cas9-Easy-lentivirus vector. .. The CNE2 cells were collected after 72 h of infection, along with the application of Mutagenesis:Article Title: The Borrelia burgdorferi Glycosaminoglycan Binding Protein Bgp in the B31 Strain Is Not Essential for Infectivity despite Facilitating Adherence and Tissue Colonization Article Snippet: .. Genomic DNA from the bgp mutant strain was isolated by standard Isolation:Article Title: The Borrelia burgdorferi Glycosaminoglycan Binding Protein Bgp in the B31 Strain Is Not Essential for Infectivity despite Facilitating Adherence and Tissue Colonization Article Snippet: .. Genomic DNA from the bgp mutant strain was isolated by standard Article Title: Rapid Identification of Methylase Specificity (RIMS-seq) jointly identifies methylated motifs and generates shotgun sequencing of bacterial genomes Article Snippet: E. coli K12 MG1655 genomic DNA was extracted from a cell culture using the DNEasy Blood and Tissue kit (69504, Qiagen). .. All the other gDNA from the bacteria presented in were isolated using the DNA Purification:Article Title: The Borrelia burgdorferi Glycosaminoglycan Binding Protein Bgp in the B31 Strain Is Not Essential for Infectivity despite Facilitating Adherence and Tissue Colonization Article Snippet: .. Genomic DNA from the bgp mutant strain was isolated by standard Article Title: A High-Efficacy CRISPR Interference System for Gene Function Discovery in Zymomonas mobilis Article Snippet: Oligonucleotides (2 μM each) were annealed in 1× CutSmart buffer (NEB) at 95°C for 5 min, followed by cooling to room temperature. .. For method two, fragments were amplified by PCR with primers oJMP197 and oJMP198 from a 78-nt oligonucleotide, followed by digestion with BsaI-HF-v2 (catalog number R3733; NEB) and purification with the Article Title: A New Protocol for Targeted insertion using CRISPR-Cas9, Oligo Single-Stranded DNA and Protoplast Regeneration Article Snippet: .. Whole genome sequencing for off-targeted insertion analysis Leaves of N. benthamiana protoplast regenerants were collected for Article Title: Rapid Identification of Methylase Specificity (RIMS-seq) jointly identifies methylated motifs and generates shotgun sequencing of bacterial genomes Article Snippet: E. coli K12 MG1655 genomic DNA was extracted from a cell culture using the DNEasy Blood and Tissue kit (69504, Qiagen). .. All the other gDNA from the bacteria presented in were isolated using the Clone Assay:Article Title: The Borrelia burgdorferi Glycosaminoglycan Binding Protein Bgp in the B31 Strain Is Not Essential for Infectivity despite Facilitating Adherence and Tissue Colonization Article Snippet: .. Genomic DNA from the bgp mutant strain was isolated by standard Sequencing:Article Title: A New Protocol for Targeted insertion using CRISPR-Cas9, Oligo Single-Stranded DNA and Protoplast Regeneration Article Snippet: .. Whole genome sequencing for off-targeted insertion analysis Leaves of N. benthamiana protoplast regenerants were collected for |