Journal: PLoS ONE

Article Title: Quantitative Analysis of Food and Feed Samples with Droplet Digital PCR

doi: 10.1371/journal.pone.0062583

Figure Lengend Snippet: MON810 content measured by ddPCR in five series of seven replicates. The aggregate represents the sum of the five series. The target certified MON810 content (0.77%) is indicated by a dotted line. Acceptance criterion for repeatability is ±25% of the target content (from 0.58% to 0.96%) represented by the dashed lines. Error bars represent the standard deviation between the replicates for each series or in the aggregate.

Article Snippet: Enzymatic digestion of MON810 genomic DNA (gDNA) with Taq I (New England Biolabs GmbH, Frankfurt am Main, Germany) was performed as described (see ) .

Techniques: Standard Deviation

Journal: PLoS ONE

Article Title: Quantitative Analysis of Food and Feed Samples with Droplet Digital PCR

doi: 10.1371/journal.pone.0062583

Figure Lengend Snippet: MON810 content measured by ddPCR in five series of seven target concentrations. The target MON810 content (3.85%) is indicated by a dotted line. Acceptance criterion for precision is ±25% of the target content (from 2.89% to 4.81%) represented by the dashed lines. Error bars represent the standard deviation of the measured MON810% by ddPCR at each target concentration (five replicates per target concentration).

Article Snippet: Enzymatic digestion of MON810 genomic DNA (gDNA) with Taq I (New England Biolabs GmbH, Frankfurt am Main, Germany) was performed as described (see ) .

Techniques: Standard Deviation, Concentration Assay

Journal: PLoS ONE

Article Title: Quantitative Analysis of Food and Feed Samples with Droplet Digital PCR

doi: 10.1371/journal.pone.0062583

Figure Lengend Snippet: Summary table of qPCR, ddPCR and cdPCR performance for MON810 detection and quantification.

Article Snippet: Enzymatic digestion of MON810 genomic DNA (gDNA) with Taq I (New England Biolabs GmbH, Frankfurt am Main, Germany) was performed as described (see ) .

Techniques: Plasmid Preparation, Inhibition, DNA Extraction

Journal: International Journal of Molecular Sciences

Article Title: Dek504 Encodes a Mitochondrion-Targeted E+-Type Pentatricopeptide Repeat Protein Essential for RNA Editing and Seed Development in Maize

doi: 10.3390/ijms23052513

Figure Lengend Snippet: DEK504 is required for nad3 -44 and nad3 -317 C-to-U editing in maize mitochondria. ( a ) Analysis of RNA editing in nad3 transcripts of wildtype (WT) and dek504-ref kernels. The editing sites are indicated by the red lines. Codons harboring the edited nucleotides and coded amino acids are shown. ( b ) Alignment of amino acid residues at positions 6 and 1′ in each PPR motif of DEK504 with a −1 to −15 bp upstream sequence of these four defective editing sites. Nucleotides matching the recognition code of DEK504 in nad3 are shown in blue. The edited C residues in nad3 are shown in red. ( c ) Alignment of the neighboring gDNA sequences of nad3. The gDNA and cDNA sequences were obtained from the NCBI database. Monocots included Zea luxurians , Hordeum vulgare , Sorghum bicolor , Triticum aestivum , Bambusa oldhamii , Avena sativa , Phoenix dactylifera , Stipa capillata , Allium cepa , and Coix lacryma-jobi . Dicots-I included Arabidopsis thaliana , Brassica juncea , Brassica rapa , Brassica napus , and Boechera stricta . Dicots-II included Gossypium trilobum , Lagerstroemia indica , Bombax ceiba , Fagus sylvatica , Manihot esculenta , Heuchera parviflora , Malania oleifera , and Damnacanthus indicus . Dicots-III included Cannabis sativa , Panax vietnamensis , Panax notoginseng , and Tamarindus indica. Red triangles indicate the positions affected by nad3 -44 and nad3 -317.

Article Snippet: RNA was extracted using a commercial kit (Tiangen, Beijing, China), and residual gDNA was removed with RNAse-free DNAse I (NEB) treatment.

Techniques: Sequencing