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monarch rna cleanup kit  (New England Biolabs)


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    New England Biolabs monarch rna cleanup kit
    Target identification of AbnZ via two orthogonal approaches. ( A ) Schematic representation of the experimental target identification. For AbnZ pulse-overexpression (left side), C. crescentus Δ vanAB cells harbouring pSOEP-EV or pSOEP-AbnZ were cultivated in triplicates to an OD 660 of 0.4 and <t>RNA</t> samples were collected 15 min after addition of 0.5 mM VAN. For the AbnZ affinity pull-down (right), C. crescentus Δ vanAB pSOEP-AbnZ cells were cultivated in duplicates to an OD 660 of 0.4 when 0.5 mM VAN was added to the culture. After 15 min, cells were UV-crosslinked. AbnZ and bound RNAs were recovered by affinity purification using biotinylated antisense oligos. RNA recovered from both experimental strategies was converted <t>to</t> <t>cDNA</t> and subjected to HTPS. ( B ) Analysis of AbnZ pulse-overexpression. Differentially expressed genes (≥2-fold regulation; FDR-adjusted P -value ≤ 0.05) are highlighted in the volcano plot. ( C ) Venn diagram summarizing the overlap of differentially expressed genes recovered in the AbnZ pulse-overexpression and the AbnZ affinity pull-down, respectively. The six genes identified by both approaches are listed. ( D ) cDNA reads of affinity pull-down libraries mapping to the abnZ and tamAB loci, respectively. Read mappings obtained from two biological replicates using a control oligo (c I, c II) or AbnZ-specific oligos (AbnZ I, AbnZ II) are plotted.
    Monarch Rna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch rna cleanup kit/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch rna cleanup kit - by Bioz Stars, 2025-01
    86/100 stars

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    1) Product Images from "An sRNA overexpression library reveals AbnZ as a negative regulator of an essential translocation module in Caulobacter crescentus"

    Article Title: An sRNA overexpression library reveals AbnZ as a negative regulator of an essential translocation module in Caulobacter crescentus

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkae1139

    Target identification of AbnZ via two orthogonal approaches. ( A ) Schematic representation of the experimental target identification. For AbnZ pulse-overexpression (left side), C. crescentus Δ vanAB cells harbouring pSOEP-EV or pSOEP-AbnZ were cultivated in triplicates to an OD 660 of 0.4 and RNA samples were collected 15 min after addition of 0.5 mM VAN. For the AbnZ affinity pull-down (right), C. crescentus Δ vanAB pSOEP-AbnZ cells were cultivated in duplicates to an OD 660 of 0.4 when 0.5 mM VAN was added to the culture. After 15 min, cells were UV-crosslinked. AbnZ and bound RNAs were recovered by affinity purification using biotinylated antisense oligos. RNA recovered from both experimental strategies was converted to cDNA and subjected to HTPS. ( B ) Analysis of AbnZ pulse-overexpression. Differentially expressed genes (≥2-fold regulation; FDR-adjusted P -value ≤ 0.05) are highlighted in the volcano plot. ( C ) Venn diagram summarizing the overlap of differentially expressed genes recovered in the AbnZ pulse-overexpression and the AbnZ affinity pull-down, respectively. The six genes identified by both approaches are listed. ( D ) cDNA reads of affinity pull-down libraries mapping to the abnZ and tamAB loci, respectively. Read mappings obtained from two biological replicates using a control oligo (c I, c II) or AbnZ-specific oligos (AbnZ I, AbnZ II) are plotted.
    Figure Legend Snippet: Target identification of AbnZ via two orthogonal approaches. ( A ) Schematic representation of the experimental target identification. For AbnZ pulse-overexpression (left side), C. crescentus Δ vanAB cells harbouring pSOEP-EV or pSOEP-AbnZ were cultivated in triplicates to an OD 660 of 0.4 and RNA samples were collected 15 min after addition of 0.5 mM VAN. For the AbnZ affinity pull-down (right), C. crescentus Δ vanAB pSOEP-AbnZ cells were cultivated in duplicates to an OD 660 of 0.4 when 0.5 mM VAN was added to the culture. After 15 min, cells were UV-crosslinked. AbnZ and bound RNAs were recovered by affinity purification using biotinylated antisense oligos. RNA recovered from both experimental strategies was converted to cDNA and subjected to HTPS. ( B ) Analysis of AbnZ pulse-overexpression. Differentially expressed genes (≥2-fold regulation; FDR-adjusted P -value ≤ 0.05) are highlighted in the volcano plot. ( C ) Venn diagram summarizing the overlap of differentially expressed genes recovered in the AbnZ pulse-overexpression and the AbnZ affinity pull-down, respectively. The six genes identified by both approaches are listed. ( D ) cDNA reads of affinity pull-down libraries mapping to the abnZ and tamAB loci, respectively. Read mappings obtained from two biological replicates using a control oligo (c I, c II) or AbnZ-specific oligos (AbnZ I, AbnZ II) are plotted.

    Techniques Used: Over Expression, Affinity Purification, Control



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    monarch rna cleanup kit  (New England Biolabs)
    86
    New England Biolabs monarch rna cleanup kit
    Target identification of AbnZ via two orthogonal approaches. ( A ) Schematic representation of the experimental target identification. For AbnZ pulse-overexpression (left side), C. crescentus Δ vanAB cells harbouring pSOEP-EV or pSOEP-AbnZ were cultivated in triplicates to an OD 660 of 0.4 and <t>RNA</t> samples were collected 15 min after addition of 0.5 mM VAN. For the AbnZ affinity pull-down (right), C. crescentus Δ vanAB pSOEP-AbnZ cells were cultivated in duplicates to an OD 660 of 0.4 when 0.5 mM VAN was added to the culture. After 15 min, cells were UV-crosslinked. AbnZ and bound RNAs were recovered by affinity purification using biotinylated antisense oligos. RNA recovered from both experimental strategies was converted <t>to</t> <t>cDNA</t> and subjected to HTPS. ( B ) Analysis of AbnZ pulse-overexpression. Differentially expressed genes (≥2-fold regulation; FDR-adjusted P -value ≤ 0.05) are highlighted in the volcano plot. ( C ) Venn diagram summarizing the overlap of differentially expressed genes recovered in the AbnZ pulse-overexpression and the AbnZ affinity pull-down, respectively. The six genes identified by both approaches are listed. ( D ) cDNA reads of affinity pull-down libraries mapping to the abnZ and tamAB loci, respectively. Read mappings obtained from two biological replicates using a control oligo (c I, c II) or AbnZ-specific oligos (AbnZ I, AbnZ II) are plotted.
    Monarch Rna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch rna cleanup kit/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch rna cleanup kit - by Bioz Stars, 2025-01
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Target identification of AbnZ via two orthogonal approaches. ( A ) Schematic representation of the experimental target identification. For AbnZ pulse-overexpression (left side), C. crescentus Δ vanAB cells harbouring pSOEP-EV or pSOEP-AbnZ were cultivated in triplicates to an OD 660 of 0.4 and RNA samples were collected 15 min after addition of 0.5 mM VAN. For the AbnZ affinity pull-down (right), C. crescentus Δ vanAB pSOEP-AbnZ cells were cultivated in duplicates to an OD 660 of 0.4 when 0.5 mM VAN was added to the culture. After 15 min, cells were UV-crosslinked. AbnZ and bound RNAs were recovered by affinity purification using biotinylated antisense oligos. RNA recovered from both experimental strategies was converted to cDNA and subjected to HTPS. ( B ) Analysis of AbnZ pulse-overexpression. Differentially expressed genes (≥2-fold regulation; FDR-adjusted P -value ≤ 0.05) are highlighted in the volcano plot. ( C ) Venn diagram summarizing the overlap of differentially expressed genes recovered in the AbnZ pulse-overexpression and the AbnZ affinity pull-down, respectively. The six genes identified by both approaches are listed. ( D ) cDNA reads of affinity pull-down libraries mapping to the abnZ and tamAB loci, respectively. Read mappings obtained from two biological replicates using a control oligo (c I, c II) or AbnZ-specific oligos (AbnZ I, AbnZ II) are plotted.

    Journal: Nucleic Acids Research

    Article Title: An sRNA overexpression library reveals AbnZ as a negative regulator of an essential translocation module in Caulobacter crescentus

    doi: 10.1093/nar/gkae1139

    Figure Lengend Snippet: Target identification of AbnZ via two orthogonal approaches. ( A ) Schematic representation of the experimental target identification. For AbnZ pulse-overexpression (left side), C. crescentus Δ vanAB cells harbouring pSOEP-EV or pSOEP-AbnZ were cultivated in triplicates to an OD 660 of 0.4 and RNA samples were collected 15 min after addition of 0.5 mM VAN. For the AbnZ affinity pull-down (right), C. crescentus Δ vanAB pSOEP-AbnZ cells were cultivated in duplicates to an OD 660 of 0.4 when 0.5 mM VAN was added to the culture. After 15 min, cells were UV-crosslinked. AbnZ and bound RNAs were recovered by affinity purification using biotinylated antisense oligos. RNA recovered from both experimental strategies was converted to cDNA and subjected to HTPS. ( B ) Analysis of AbnZ pulse-overexpression. Differentially expressed genes (≥2-fold regulation; FDR-adjusted P -value ≤ 0.05) are highlighted in the volcano plot. ( C ) Venn diagram summarizing the overlap of differentially expressed genes recovered in the AbnZ pulse-overexpression and the AbnZ affinity pull-down, respectively. The six genes identified by both approaches are listed. ( D ) cDNA reads of affinity pull-down libraries mapping to the abnZ and tamAB loci, respectively. Read mappings obtained from two biological replicates using a control oligo (c I, c II) or AbnZ-specific oligos (AbnZ I, AbnZ II) are plotted.

    Article Snippet: Recovered RNA was fragmented using the NEBNext® Magnesium RNA Fragmentation Module (NEB, E6150) and purified with the Monarch® RNA Cleanup Kit (NEB, T2040). cDNA libraries were prepared with the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, E7300) according to the manufacturer's instructions, and when necessary, library size was adjusted using AMPure XP Beads (Beckmann Coulter).

    Techniques: Over Expression, Affinity Purification, Control