2586 er 3  (ATCC)


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    ATCC 2586 er 3
    2586 Er 3, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    2586 er 3  (ATCC)


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    ATCC 2586 er 3
    2586 Er 3, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    b dermatitidis ps1  (ATCC)


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    ATCC b dermatitidis ps1
    Summary of the MLST locus characteristics applied to 78 <t> B. dermatitidis </t> isolates.
    B Dermatitidis Ps1, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Phylogenetic Analysis Reveals a Cryptic Species Blastomyces gilchristii , sp. nov. within the Human Pathogenic Fungus Blastomyces dermatitidis"

    Article Title: Phylogenetic Analysis Reveals a Cryptic Species Blastomyces gilchristii , sp. nov. within the Human Pathogenic Fungus Blastomyces dermatitidis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0059237

    Summary of the MLST locus characteristics applied to 78  B. dermatitidis  isolates.
    Figure Legend Snippet: Summary of the MLST locus characteristics applied to 78 B. dermatitidis isolates.

    Techniques Used:

    A majority consensus mid-point rooted maximum parsimony (MP) tree was constructed based on the concatenated sequence of seven MLST gene loci. The MP tree is displayed without logarithmic scaling so that genetic distance and geographic region of isolation could be viewed. Values along branches represent maximum parsimony bootstrap values (MPB) and Bayesian posterior probability (BPP) values respectively. Values for branches partitioning less than three isolates or those with MPB ≤70 and BPP≤0.95 are not shown. The tree is displayed with pattern coding for geographic regions as described in . The MP tree displays a partition in the current species B. dermatitidis into two phylogenetic species: B. dermatitidis (PS1, clade 1) and B. gilchristii (PS2, clade 2).
    Figure Legend Snippet: A majority consensus mid-point rooted maximum parsimony (MP) tree was constructed based on the concatenated sequence of seven MLST gene loci. The MP tree is displayed without logarithmic scaling so that genetic distance and geographic region of isolation could be viewed. Values along branches represent maximum parsimony bootstrap values (MPB) and Bayesian posterior probability (BPP) values respectively. Values for branches partitioning less than three isolates or those with MPB ≤70 and BPP≤0.95 are not shown. The tree is displayed with pattern coding for geographic regions as described in . The MP tree displays a partition in the current species B. dermatitidis into two phylogenetic species: B. dermatitidis (PS1, clade 1) and B. gilchristii (PS2, clade 2).

    Techniques Used: Construct, Sequencing, Isolation

    Linkage disequilibrium measurements for all isolates and unique genotypes in the entire population, the entire population partitioned into two phylogenetic species or six geographic regions, and the population within each phylogenetic species (  B. dermatitidis (PS1)  and B. gilchristii (PS2)).
    Figure Legend Snippet: Linkage disequilibrium measurements for all isolates and unique genotypes in the entire population, the entire population partitioned into two phylogenetic species or six geographic regions, and the population within each phylogenetic species ( B. dermatitidis (PS1) and B. gilchristii (PS2)).

    Techniques Used:

    A frequency distribution of tree lengths of maximum parsimony trees for 1000 artificially recombined datasets as compared to the observed tree length was calculated for: (a) All isolates in the B. dermatitidis population treated as a single population both with and without reduction to unique genotypes; (b) All isolates partitioned into two phylogenetic species (PS1) and (PS2) both with and without reduction to unique genotypes; (c) All isolates partitioned into 6 geographic regions both with and without reduction to unique genotypes; (d) Isolates of PS1 treated as a single population, partitioned into 6 geographic regions both with and without reduction to unique genotypes; (e) Isolates of PS2 treated as a single population, partitioned into 4 geographic regions, or reduced to unique genotypes (due to the low number of unique genotypes in PS2, we were unable to partition them by geographic region). Recombination was not detected between PS1 and PS2. Recombination was detected in PS1 (d) when partitioned by geographic region and reduced to unique genotypes and in PS2 (e) when reduced to unique genotypes.
    Figure Legend Snippet: A frequency distribution of tree lengths of maximum parsimony trees for 1000 artificially recombined datasets as compared to the observed tree length was calculated for: (a) All isolates in the B. dermatitidis population treated as a single population both with and without reduction to unique genotypes; (b) All isolates partitioned into two phylogenetic species (PS1) and (PS2) both with and without reduction to unique genotypes; (c) All isolates partitioned into 6 geographic regions both with and without reduction to unique genotypes; (d) Isolates of PS1 treated as a single population, partitioned into 6 geographic regions both with and without reduction to unique genotypes; (e) Isolates of PS2 treated as a single population, partitioned into 4 geographic regions, or reduced to unique genotypes (due to the low number of unique genotypes in PS2, we were unable to partition them by geographic region). Recombination was not detected between PS1 and PS2. Recombination was detected in PS1 (d) when partitioned by geographic region and reduced to unique genotypes and in PS2 (e) when reduced to unique genotypes.

    Techniques Used:

    of the B. dermatitidis. Split decomposition analysis was performed using the pairwise genetic distances of the concatenated chs2-drk1-fads-pyrF-tub1-arf6-its2 sequences. Isolated split networks within B. gilchristii (PS2) suggests recombination within the B. gilchristii (PS2) but not between both populations. Bootstrapping values less than ≤0.75 are not shown.
    Figure Legend Snippet: of the B. dermatitidis. Split decomposition analysis was performed using the pairwise genetic distances of the concatenated chs2-drk1-fads-pyrF-tub1-arf6-its2 sequences. Isolated split networks within B. gilchristii (PS2) suggests recombination within the B. gilchristii (PS2) but not between both populations. Bootstrapping values less than ≤0.75 are not shown.

    Techniques Used: Isolation

    Intra- and interlocus simple four-gametes tests of each locus for  B. dermatitidis (PS1)  and B. gilchristii (PS2).
    Figure Legend Snippet: Intra- and interlocus simple four-gametes tests of each locus for B. dermatitidis (PS1) and B. gilchristii (PS2).

    Techniques Used:

    Phylogenetic species are represented by circles with light grey ( B. dermatitidis , PS1) and solid ( B. gilchristii , PS2) fill corresponding to each phylogenetic species. Circle size is proportional to the number of isolates from each geographic site.
    Figure Legend Snippet: Phylogenetic species are represented by circles with light grey ( B. dermatitidis , PS1) and solid ( B. gilchristii , PS2) fill corresponding to each phylogenetic species. Circle size is proportional to the number of isolates from each geographic site.

    Techniques Used:

    2586 er 3  (ATCC)


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    ATCC 2586 er 3
    2586 Er 3, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    er 3  (ATCC)


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    ATCC er 3
    Er 3, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mya 2586  (ATCC)


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    ATCC mya 2586
    Mya 2586, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    er 3  (ATCC)


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    ATCC er 3
    Er 3, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    er 3  (ATCC)


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    ATCC er 3
    Er 3, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    entrez protein  (ATCC)


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    ATCC entrez protein
    Entrez Protein, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    er3  (ATCC)


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    ATCC er3
    Er3, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    er3  (ATCC)


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    ATCC er3
    Er3, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC 2586 er 3
    2586 Er 3, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Summary of the MLST locus characteristics applied to 78 <t> B. dermatitidis </t> isolates.
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    Summary of the MLST locus characteristics applied to 78 <t> B. dermatitidis </t> isolates.
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    Summary of the MLST locus characteristics applied to 78 <t> B. dermatitidis </t> isolates.
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    Summary of the MLST locus characteristics applied to 78 <t> B. dermatitidis </t> isolates.
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    Summary of the MLST locus characteristics applied to 78 <t> B. dermatitidis </t> isolates.
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    Summary of the MLST locus characteristics applied to 78  B. dermatitidis  isolates.

    Journal: PLoS ONE

    Article Title: Phylogenetic Analysis Reveals a Cryptic Species Blastomyces gilchristii , sp. nov. within the Human Pathogenic Fungus Blastomyces dermatitidis

    doi: 10.1371/journal.pone.0059237

    Figure Lengend Snippet: Summary of the MLST locus characteristics applied to 78 B. dermatitidis isolates.

    Article Snippet: Differences in growth on various media have also been documented for select B. dermatitidis (PS1) (ATCC MYA-2586 (ER-3)) and B. gilchristii (PS2) (ATCC MYA- 2585 (ERC-2)) isolates at 20°C and 37°C .

    Techniques:

    A majority consensus mid-point rooted maximum parsimony (MP) tree was constructed based on the concatenated sequence of seven MLST gene loci. The MP tree is displayed without logarithmic scaling so that genetic distance and geographic region of isolation could be viewed. Values along branches represent maximum parsimony bootstrap values (MPB) and Bayesian posterior probability (BPP) values respectively. Values for branches partitioning less than three isolates or those with MPB ≤70 and BPP≤0.95 are not shown. The tree is displayed with pattern coding for geographic regions as described in . The MP tree displays a partition in the current species B. dermatitidis into two phylogenetic species: B. dermatitidis (PS1, clade 1) and B. gilchristii (PS2, clade 2).

    Journal: PLoS ONE

    Article Title: Phylogenetic Analysis Reveals a Cryptic Species Blastomyces gilchristii , sp. nov. within the Human Pathogenic Fungus Blastomyces dermatitidis

    doi: 10.1371/journal.pone.0059237

    Figure Lengend Snippet: A majority consensus mid-point rooted maximum parsimony (MP) tree was constructed based on the concatenated sequence of seven MLST gene loci. The MP tree is displayed without logarithmic scaling so that genetic distance and geographic region of isolation could be viewed. Values along branches represent maximum parsimony bootstrap values (MPB) and Bayesian posterior probability (BPP) values respectively. Values for branches partitioning less than three isolates or those with MPB ≤70 and BPP≤0.95 are not shown. The tree is displayed with pattern coding for geographic regions as described in . The MP tree displays a partition in the current species B. dermatitidis into two phylogenetic species: B. dermatitidis (PS1, clade 1) and B. gilchristii (PS2, clade 2).

    Article Snippet: Differences in growth on various media have also been documented for select B. dermatitidis (PS1) (ATCC MYA-2586 (ER-3)) and B. gilchristii (PS2) (ATCC MYA- 2585 (ERC-2)) isolates at 20°C and 37°C .

    Techniques: Construct, Sequencing, Isolation

    Linkage disequilibrium measurements for all isolates and unique genotypes in the entire population, the entire population partitioned into two phylogenetic species or six geographic regions, and the population within each phylogenetic species (  B. dermatitidis (PS1)  and B. gilchristii (PS2)).

    Journal: PLoS ONE

    Article Title: Phylogenetic Analysis Reveals a Cryptic Species Blastomyces gilchristii , sp. nov. within the Human Pathogenic Fungus Blastomyces dermatitidis

    doi: 10.1371/journal.pone.0059237

    Figure Lengend Snippet: Linkage disequilibrium measurements for all isolates and unique genotypes in the entire population, the entire population partitioned into two phylogenetic species or six geographic regions, and the population within each phylogenetic species ( B. dermatitidis (PS1) and B. gilchristii (PS2)).

    Article Snippet: Differences in growth on various media have also been documented for select B. dermatitidis (PS1) (ATCC MYA-2586 (ER-3)) and B. gilchristii (PS2) (ATCC MYA- 2585 (ERC-2)) isolates at 20°C and 37°C .

    Techniques:

    A frequency distribution of tree lengths of maximum parsimony trees for 1000 artificially recombined datasets as compared to the observed tree length was calculated for: (a) All isolates in the B. dermatitidis population treated as a single population both with and without reduction to unique genotypes; (b) All isolates partitioned into two phylogenetic species (PS1) and (PS2) both with and without reduction to unique genotypes; (c) All isolates partitioned into 6 geographic regions both with and without reduction to unique genotypes; (d) Isolates of PS1 treated as a single population, partitioned into 6 geographic regions both with and without reduction to unique genotypes; (e) Isolates of PS2 treated as a single population, partitioned into 4 geographic regions, or reduced to unique genotypes (due to the low number of unique genotypes in PS2, we were unable to partition them by geographic region). Recombination was not detected between PS1 and PS2. Recombination was detected in PS1 (d) when partitioned by geographic region and reduced to unique genotypes and in PS2 (e) when reduced to unique genotypes.

    Journal: PLoS ONE

    Article Title: Phylogenetic Analysis Reveals a Cryptic Species Blastomyces gilchristii , sp. nov. within the Human Pathogenic Fungus Blastomyces dermatitidis

    doi: 10.1371/journal.pone.0059237

    Figure Lengend Snippet: A frequency distribution of tree lengths of maximum parsimony trees for 1000 artificially recombined datasets as compared to the observed tree length was calculated for: (a) All isolates in the B. dermatitidis population treated as a single population both with and without reduction to unique genotypes; (b) All isolates partitioned into two phylogenetic species (PS1) and (PS2) both with and without reduction to unique genotypes; (c) All isolates partitioned into 6 geographic regions both with and without reduction to unique genotypes; (d) Isolates of PS1 treated as a single population, partitioned into 6 geographic regions both with and without reduction to unique genotypes; (e) Isolates of PS2 treated as a single population, partitioned into 4 geographic regions, or reduced to unique genotypes (due to the low number of unique genotypes in PS2, we were unable to partition them by geographic region). Recombination was not detected between PS1 and PS2. Recombination was detected in PS1 (d) when partitioned by geographic region and reduced to unique genotypes and in PS2 (e) when reduced to unique genotypes.

    Article Snippet: Differences in growth on various media have also been documented for select B. dermatitidis (PS1) (ATCC MYA-2586 (ER-3)) and B. gilchristii (PS2) (ATCC MYA- 2585 (ERC-2)) isolates at 20°C and 37°C .

    Techniques:

    of the B. dermatitidis. Split decomposition analysis was performed using the pairwise genetic distances of the concatenated chs2-drk1-fads-pyrF-tub1-arf6-its2 sequences. Isolated split networks within B. gilchristii (PS2) suggests recombination within the B. gilchristii (PS2) but not between both populations. Bootstrapping values less than ≤0.75 are not shown.

    Journal: PLoS ONE

    Article Title: Phylogenetic Analysis Reveals a Cryptic Species Blastomyces gilchristii , sp. nov. within the Human Pathogenic Fungus Blastomyces dermatitidis

    doi: 10.1371/journal.pone.0059237

    Figure Lengend Snippet: of the B. dermatitidis. Split decomposition analysis was performed using the pairwise genetic distances of the concatenated chs2-drk1-fads-pyrF-tub1-arf6-its2 sequences. Isolated split networks within B. gilchristii (PS2) suggests recombination within the B. gilchristii (PS2) but not between both populations. Bootstrapping values less than ≤0.75 are not shown.

    Article Snippet: Differences in growth on various media have also been documented for select B. dermatitidis (PS1) (ATCC MYA-2586 (ER-3)) and B. gilchristii (PS2) (ATCC MYA- 2585 (ERC-2)) isolates at 20°C and 37°C .

    Techniques: Isolation

    Intra- and interlocus simple four-gametes tests of each locus for  B. dermatitidis (PS1)  and B. gilchristii (PS2).

    Journal: PLoS ONE

    Article Title: Phylogenetic Analysis Reveals a Cryptic Species Blastomyces gilchristii , sp. nov. within the Human Pathogenic Fungus Blastomyces dermatitidis

    doi: 10.1371/journal.pone.0059237

    Figure Lengend Snippet: Intra- and interlocus simple four-gametes tests of each locus for B. dermatitidis (PS1) and B. gilchristii (PS2).

    Article Snippet: Differences in growth on various media have also been documented for select B. dermatitidis (PS1) (ATCC MYA-2586 (ER-3)) and B. gilchristii (PS2) (ATCC MYA- 2585 (ERC-2)) isolates at 20°C and 37°C .

    Techniques:

    Phylogenetic species are represented by circles with light grey ( B. dermatitidis , PS1) and solid ( B. gilchristii , PS2) fill corresponding to each phylogenetic species. Circle size is proportional to the number of isolates from each geographic site.

    Journal: PLoS ONE

    Article Title: Phylogenetic Analysis Reveals a Cryptic Species Blastomyces gilchristii , sp. nov. within the Human Pathogenic Fungus Blastomyces dermatitidis

    doi: 10.1371/journal.pone.0059237

    Figure Lengend Snippet: Phylogenetic species are represented by circles with light grey ( B. dermatitidis , PS1) and solid ( B. gilchristii , PS2) fill corresponding to each phylogenetic species. Circle size is proportional to the number of isolates from each geographic site.

    Article Snippet: Differences in growth on various media have also been documented for select B. dermatitidis (PS1) (ATCC MYA-2586 (ER-3)) and B. gilchristii (PS2) (ATCC MYA- 2585 (ERC-2)) isolates at 20°C and 37°C .

    Techniques: