phytophthora parasitica strain pp016  (ATCC)

Journal: Plant Physiology

Article Title: miR159 Represses a Constitutive Pathogen Defense Response in Tobacco

doi: 10.1104/pp.19.00786

Figure Lengend Snippet: P. parasitica infection of susceptible and resistant tobacco cultivars alongside transformed MIM159 lines. A to E, Leaves 3 d postinoculation with agar (Control) on one side and P. parasitica hyphae on the other are shown for susceptible tobacco NT90 (A), resistant tobacco NC2326 (B), MIM159 transgenic line L2 (C), MIM159 transgenic line L6 (D), and MIM159 transgenic line L12 (E). Scale bars = 10 mm. Asterisks in A–C indicate cell death associated with wounding or HR. F, Percentage of infection in leaves inoculated with P. parasitica or control V8 agar (n = 12–15).

Article Snippet: Tobacco Infection Assay Phytophthora parasitica isolate H1111 (ATCC MYA-141, also known as Phytophthora nicotianae ), initially isolated by Dr. David Guest (University of Sydney), was grown on V8 agar (10% [v/v] V8 juice, 1.7% [w/v] agar, 0.01% [w/v] CaCO 3 , and 0.002% [v/v] β-sitosterol) at 25°C for 4–6 d in the dark.

Techniques: Infection, Transformation Assay, Transgenic Assay