Journal: Cell reports

Article Title: Zinc Finger RNA-Binding Protein Zn72D Regulates ADAR-Mediated RNA Editing in Neurons

doi: 10.1016/j.celrep.2020.107654

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Primary mouse cortical neurons of E16.5 Mus musculus (strain: C57BL/6J) from whole mixed-sexed litters were dissociated and mixed into single cell suspensions using a papain dissociation system (Worthington Biochemical Corporation).

Techniques: Generated, Recombinant, shRNA, Expressing, Software

Journal: Journal of Innate Immunity

Article Title: Decreased Neutrophil Extracellular Trap Degradation in Shiga Toxin-Associated Haemolytic Uraemic Syndrome

doi: 10.1159/000450609

Figure Lengend Snippet: Degradation of NETs (a) and total nuclease activity (b, c) by 10% serum from patients with acute STEC-HUS (n = 30) compared to matched healthy controls (n = 24). b Representative DNA zymogram depicting the nuclease activity in TMA patients 1–3 and the internal control (NHS). Nuclease activity is found in two forms in serum, i.e. free DNase-I (arrow 2) and DNase-I in complex with a serum protein (arrow 1). d Titres of anti-dsDNA antibodies in STEC-HUS patients with acute disease (n = 30) compared to age-matched healthy controls (n = 20). e, f Correlation between NET degradation, anti-dsDNA titres and nuclease activity in STEC-HUS patients with acute disease. g NET degradation by 5% serum from low-degrading STEC-HUS patients (n = 15) compared to serum supplemented with 2 mU DNase-I or 2 mU MNase. h Levels of serum DNA in STEC-HUS patients with low (n = 15) and normal (n = 15) NET degradation and matched controls (n = 9). The significance of differences between groups, compared to healthy controls, was calculated using a Mann-Whitney U test, and for multiple groups a Kruskal-Wallis test followed by Dunn's multiple-comparisons test was used. Correlations were calculated using a non-parametric Spearman's correlation test. p values above each group indicate the statistical difference compared to healthy controls.

Article Snippet: The cell medium was removed from NETs and 5–10% patient or control sera, as indicated, in 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 10 mM MgCl 2 and 2 mM CaCl 2 (DNase buffer) was added with or without 2 mU recombinant human DNase-I (Bioworld) or 2 mU micrococcal nuclease (MNase; Worthington Biochemical Corporation) and incubated for 60 min at 37°C.

Techniques: Activity Assay, MANN-WHITNEY