bone ecm  (Xylyx Bio)


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    Xylyx Bio bone ecm
    Bone Ecm, supplied by Xylyx Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bone ecm/product/Xylyx Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bone ecm - by Bioz Stars, 2023-11
    93/100 stars

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    bone ecm  (Xylyx Bio)


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    Xylyx Bio bone ecm
    Bone Ecm, supplied by Xylyx Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bone ecm/product/Xylyx Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bone ecm - by Bioz Stars, 2023-11
    93/100 stars

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    tissuespec bone  (Xylyx Bio)


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    Xylyx Bio tissuespec bone
    Tissuespec Bone, supplied by Xylyx Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tissuespec bone/product/Xylyx Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tissuespec bone - by Bioz Stars, 2023-11
    93/100 stars

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    site metastasis kit  (Xylyx Bio)


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    Xylyx Bio site metastasis kit
    Site Metastasis Kit, supplied by Xylyx Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/site metastasis kit/product/Xylyx Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    site metastasis kit - by Bioz Stars, 2023-11
    93/100 stars

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    site tm metastasis kit  (Xylyx Bio)


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    Xylyx Bio site tm metastasis kit
    Site Tm Metastasis Kit, supplied by Xylyx Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/site tm metastasis kit/product/Xylyx Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    site tm metastasis kit - by Bioz Stars, 2023-11
    93/100 stars

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    tissuespec bone ecm hydrogel  (Xylyx Bio)


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    Xylyx Bio tissuespec bone ecm hydrogel
    Tissuespec Bone Ecm Hydrogel, supplied by Xylyx Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tissuespec bone ecm hydrogel/product/Xylyx Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tissuespec bone ecm hydrogel - by Bioz Stars, 2023-11
    94/100 stars

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    tissuespec bone  (Xylyx Bio)


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    Xylyx Bio tissuespec bone
    Clinical relevance of combining XPO1 inhibitors with current therapies in metastatic ER + tumors: Impact on metastasis related gene expression and therapy resistant cells. ( A ) Correlation between XPO1 and PIK3CA expression in ER + tumors in METABRIC and TCGA datasets. Spearman and Pearson correlation analyses coefficients and p -values were indicated for each dataset (METABRIC data set: Spearman correlation = 0.35, Pearson correlation = 0.37, TCGA data set: Spearman correlation = 0.5, Pearson correlation = 0.44). ( B ) RNA-Seq analysis in MCF7- ESR1 Y537S cells, treated with 1 µM 4-OHT, Fulv or Palb in the presence or absence of 100 nM SEL. PCA plot showing distinct gene expression patterns with the different treatments are shown at the right bottom panel of (B). ( C ) Gene-set enrichment analysis (GSEA) of gene sets that were enriched in 4-OHT + SEL dataset. ( D ) Cell viability assay in different endocrine-sensitive or endocrine-resistant breast cancer cell lines (MCF7 Parental, MCF7- ESR1 Y537S , MCF7- ESR1 D538G , T47D Parental, T47D- ESR1 Y537S , T47D- ESR1 D538G , MCF7 cells treated with different treatments and BT474), cotreated with 4-OHT [10 −6 M], Fulv [10 −6 M] and Palb [10 −6 M] and SEL [10 −7 M]. A one-way analysis of variance (ANOVA) model was used for statistical significance of change in cell viability with different treatments. All values were presented as mean ± SEM from six independent repeats. ( E ) Scanning electron microscopy (SEM) images of human tissues and decellularized tissue from which ECM hydrogels are derived. ( F ) Stiffness of <t>TissueSpec</t> ® ECM Hydrogels. ( G ). MCF7- ESR1 Y537S cells were cultured at a density of 2 × 10 3 cells/well and embedded in 3D hydrogels to mimic different metastatic niches (bone, liver, lung) for breast cancer cells, and they were treated with different endocrine agents (4OHT [10 −6 ], Fulv [10 −6 ] and Palb [10 −6 ]) and in combination with SEL [10 −7 ]. ( H ). Colony size (pixels) and number were quantified using OpenCFU colony counting software ( http://opencfu.sourceforge.net/ ). A one-way analysis of variance (ANOVA) model was used for statistical significance of treatment and values were presented as mean ± SEM from three biological replicates (** p <0.01, *** p <0.001; **** p < 0.0001).
    Tissuespec Bone, supplied by Xylyx Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tissuespec bone/product/Xylyx Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tissuespec bone - by Bioz Stars, 2023-11
    93/100 stars

    Images

    1) Product Images from "Combined Targeting of Estrogen Receptor Alpha and Exportin 1 in Metastatic Breast Cancers"

    Article Title: Combined Targeting of Estrogen Receptor Alpha and Exportin 1 in Metastatic Breast Cancers

    Journal: Cancers

    doi: 10.3390/cancers12092397

    Clinical relevance of combining XPO1 inhibitors with current therapies in metastatic ER + tumors: Impact on metastasis related gene expression and therapy resistant cells. ( A ) Correlation between XPO1 and PIK3CA expression in ER + tumors in METABRIC and TCGA datasets. Spearman and Pearson correlation analyses coefficients and p -values were indicated for each dataset (METABRIC data set: Spearman correlation = 0.35, Pearson correlation = 0.37, TCGA data set: Spearman correlation = 0.5, Pearson correlation = 0.44). ( B ) RNA-Seq analysis in MCF7- ESR1 Y537S cells, treated with 1 µM 4-OHT, Fulv or Palb in the presence or absence of 100 nM SEL. PCA plot showing distinct gene expression patterns with the different treatments are shown at the right bottom panel of (B). ( C ) Gene-set enrichment analysis (GSEA) of gene sets that were enriched in 4-OHT + SEL dataset. ( D ) Cell viability assay in different endocrine-sensitive or endocrine-resistant breast cancer cell lines (MCF7 Parental, MCF7- ESR1 Y537S , MCF7- ESR1 D538G , T47D Parental, T47D- ESR1 Y537S , T47D- ESR1 D538G , MCF7 cells treated with different treatments and BT474), cotreated with 4-OHT [10 −6 M], Fulv [10 −6 M] and Palb [10 −6 M] and SEL [10 −7 M]. A one-way analysis of variance (ANOVA) model was used for statistical significance of change in cell viability with different treatments. All values were presented as mean ± SEM from six independent repeats. ( E ) Scanning electron microscopy (SEM) images of human tissues and decellularized tissue from which ECM hydrogels are derived. ( F ) Stiffness of TissueSpec ® ECM Hydrogels. ( G ). MCF7- ESR1 Y537S cells were cultured at a density of 2 × 10 3 cells/well and embedded in 3D hydrogels to mimic different metastatic niches (bone, liver, lung) for breast cancer cells, and they were treated with different endocrine agents (4OHT [10 −6 ], Fulv [10 −6 ] and Palb [10 −6 ]) and in combination with SEL [10 −7 ]. ( H ). Colony size (pixels) and number were quantified using OpenCFU colony counting software ( http://opencfu.sourceforge.net/ ). A one-way analysis of variance (ANOVA) model was used for statistical significance of treatment and values were presented as mean ± SEM from three biological replicates (** p <0.01, *** p <0.001; **** p < 0.0001).
    Figure Legend Snippet: Clinical relevance of combining XPO1 inhibitors with current therapies in metastatic ER + tumors: Impact on metastasis related gene expression and therapy resistant cells. ( A ) Correlation between XPO1 and PIK3CA expression in ER + tumors in METABRIC and TCGA datasets. Spearman and Pearson correlation analyses coefficients and p -values were indicated for each dataset (METABRIC data set: Spearman correlation = 0.35, Pearson correlation = 0.37, TCGA data set: Spearman correlation = 0.5, Pearson correlation = 0.44). ( B ) RNA-Seq analysis in MCF7- ESR1 Y537S cells, treated with 1 µM 4-OHT, Fulv or Palb in the presence or absence of 100 nM SEL. PCA plot showing distinct gene expression patterns with the different treatments are shown at the right bottom panel of (B). ( C ) Gene-set enrichment analysis (GSEA) of gene sets that were enriched in 4-OHT + SEL dataset. ( D ) Cell viability assay in different endocrine-sensitive or endocrine-resistant breast cancer cell lines (MCF7 Parental, MCF7- ESR1 Y537S , MCF7- ESR1 D538G , T47D Parental, T47D- ESR1 Y537S , T47D- ESR1 D538G , MCF7 cells treated with different treatments and BT474), cotreated with 4-OHT [10 −6 M], Fulv [10 −6 M] and Palb [10 −6 M] and SEL [10 −7 M]. A one-way analysis of variance (ANOVA) model was used for statistical significance of change in cell viability with different treatments. All values were presented as mean ± SEM from six independent repeats. ( E ) Scanning electron microscopy (SEM) images of human tissues and decellularized tissue from which ECM hydrogels are derived. ( F ) Stiffness of TissueSpec ® ECM Hydrogels. ( G ). MCF7- ESR1 Y537S cells were cultured at a density of 2 × 10 3 cells/well and embedded in 3D hydrogels to mimic different metastatic niches (bone, liver, lung) for breast cancer cells, and they were treated with different endocrine agents (4OHT [10 −6 ], Fulv [10 −6 ] and Palb [10 −6 ]) and in combination with SEL [10 −7 ]. ( H ). Colony size (pixels) and number were quantified using OpenCFU colony counting software ( http://opencfu.sourceforge.net/ ). A one-way analysis of variance (ANOVA) model was used for statistical significance of treatment and values were presented as mean ± SEM from three biological replicates (** p <0.01, *** p <0.001; **** p < 0.0001).

    Techniques Used: Expressing, RNA Sequencing Assay, Viability Assay, Electron Microscopy, Derivative Assay, Cell Culture, Software

    tissuespec bone  (Xylyx Bio)


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    Xylyx Bio tissuespec bone
    Clinical relevance of combining XPO1 inhibitors with current therapies in metastatic ER + tumors: Impact on metastasis related gene expression and therapy resistant cells. ( A ) Correlation between XPO1 and PIK3CA expression in ER + tumors in METABRIC and TCGA datasets. Spearman and Pearson correlation analyses coefficients and p -values were indicated for each dataset (METABRIC data set: Spearman correlation = 0.35, Pearson correlation = 0.37, TCGA data set: Spearman correlation = 0.5, Pearson correlation = 0.44). ( B ) RNA-Seq analysis in MCF7- ESR1 Y537S cells, treated with 1 µM 4-OHT, Fulv or Palb in the presence or absence of 100 nM SEL. PCA plot showing distinct gene expression patterns with the different treatments are shown at the right bottom panel of (B). ( C ) Gene-set enrichment analysis (GSEA) of gene sets that were enriched in 4-OHT + SEL dataset. ( D ) Cell viability assay in different endocrine-sensitive or endocrine-resistant breast cancer cell lines (MCF7 Parental, MCF7- ESR1 Y537S , MCF7- ESR1 D538G , T47D Parental, T47D- ESR1 Y537S , T47D- ESR1 D538G , MCF7 cells treated with different treatments and BT474), cotreated with 4-OHT [10 −6 M], Fulv [10 −6 M] and Palb [10 −6 M] and SEL [10 −7 M]. A one-way analysis of variance (ANOVA) model was used for statistical significance of change in cell viability with different treatments. All values were presented as mean ± SEM from six independent repeats. ( E ) Scanning electron microscopy (SEM) images of human tissues and decellularized tissue from which ECM hydrogels are derived. ( F ) Stiffness of <t>TissueSpec</t> ® ECM Hydrogels. ( G ). MCF7- ESR1 Y537S cells were cultured at a density of 2 × 10 3 cells/well and embedded in 3D hydrogels to mimic different metastatic niches (bone, liver, lung) for breast cancer cells, and they were treated with different endocrine agents (4OHT [10 −6 ], Fulv [10 −6 ] and Palb [10 −6 ]) and in combination with SEL [10 −7 ]. ( H ). Colony size (pixels) and number were quantified using OpenCFU colony counting software ( http://opencfu.sourceforge.net/ ). A one-way analysis of variance (ANOVA) model was used for statistical significance of treatment and values were presented as mean ± SEM from three biological replicates (** p <0.01, *** p <0.001; **** p < 0.0001).
    Tissuespec Bone, supplied by Xylyx Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tissuespec bone/product/Xylyx Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tissuespec bone - by Bioz Stars, 2023-11
    93/100 stars

    Images

    1) Product Images from "Combined Targeting of Estrogen Receptor Alpha and Exportin 1 in Metastatic Breast Cancers"

    Article Title: Combined Targeting of Estrogen Receptor Alpha and Exportin 1 in Metastatic Breast Cancers

    Journal: Cancers

    doi: 10.3390/cancers12092397

    Clinical relevance of combining XPO1 inhibitors with current therapies in metastatic ER + tumors: Impact on metastasis related gene expression and therapy resistant cells. ( A ) Correlation between XPO1 and PIK3CA expression in ER + tumors in METABRIC and TCGA datasets. Spearman and Pearson correlation analyses coefficients and p -values were indicated for each dataset (METABRIC data set: Spearman correlation = 0.35, Pearson correlation = 0.37, TCGA data set: Spearman correlation = 0.5, Pearson correlation = 0.44). ( B ) RNA-Seq analysis in MCF7- ESR1 Y537S cells, treated with 1 µM 4-OHT, Fulv or Palb in the presence or absence of 100 nM SEL. PCA plot showing distinct gene expression patterns with the different treatments are shown at the right bottom panel of (B). ( C ) Gene-set enrichment analysis (GSEA) of gene sets that were enriched in 4-OHT + SEL dataset. ( D ) Cell viability assay in different endocrine-sensitive or endocrine-resistant breast cancer cell lines (MCF7 Parental, MCF7- ESR1 Y537S , MCF7- ESR1 D538G , T47D Parental, T47D- ESR1 Y537S , T47D- ESR1 D538G , MCF7 cells treated with different treatments and BT474), cotreated with 4-OHT [10 −6 M], Fulv [10 −6 M] and Palb [10 −6 M] and SEL [10 −7 M]. A one-way analysis of variance (ANOVA) model was used for statistical significance of change in cell viability with different treatments. All values were presented as mean ± SEM from six independent repeats. ( E ) Scanning electron microscopy (SEM) images of human tissues and decellularized tissue from which ECM hydrogels are derived. ( F ) Stiffness of TissueSpec ® ECM Hydrogels. ( G ). MCF7- ESR1 Y537S cells were cultured at a density of 2 × 10 3 cells/well and embedded in 3D hydrogels to mimic different metastatic niches (bone, liver, lung) for breast cancer cells, and they were treated with different endocrine agents (4OHT [10 −6 ], Fulv [10 −6 ] and Palb [10 −6 ]) and in combination with SEL [10 −7 ]. ( H ). Colony size (pixels) and number were quantified using OpenCFU colony counting software ( http://opencfu.sourceforge.net/ ). A one-way analysis of variance (ANOVA) model was used for statistical significance of treatment and values were presented as mean ± SEM from three biological replicates (** p <0.01, *** p <0.001; **** p < 0.0001).
    Figure Legend Snippet: Clinical relevance of combining XPO1 inhibitors with current therapies in metastatic ER + tumors: Impact on metastasis related gene expression and therapy resistant cells. ( A ) Correlation between XPO1 and PIK3CA expression in ER + tumors in METABRIC and TCGA datasets. Spearman and Pearson correlation analyses coefficients and p -values were indicated for each dataset (METABRIC data set: Spearman correlation = 0.35, Pearson correlation = 0.37, TCGA data set: Spearman correlation = 0.5, Pearson correlation = 0.44). ( B ) RNA-Seq analysis in MCF7- ESR1 Y537S cells, treated with 1 µM 4-OHT, Fulv or Palb in the presence or absence of 100 nM SEL. PCA plot showing distinct gene expression patterns with the different treatments are shown at the right bottom panel of (B). ( C ) Gene-set enrichment analysis (GSEA) of gene sets that were enriched in 4-OHT + SEL dataset. ( D ) Cell viability assay in different endocrine-sensitive or endocrine-resistant breast cancer cell lines (MCF7 Parental, MCF7- ESR1 Y537S , MCF7- ESR1 D538G , T47D Parental, T47D- ESR1 Y537S , T47D- ESR1 D538G , MCF7 cells treated with different treatments and BT474), cotreated with 4-OHT [10 −6 M], Fulv [10 −6 M] and Palb [10 −6 M] and SEL [10 −7 M]. A one-way analysis of variance (ANOVA) model was used for statistical significance of change in cell viability with different treatments. All values were presented as mean ± SEM from six independent repeats. ( E ) Scanning electron microscopy (SEM) images of human tissues and decellularized tissue from which ECM hydrogels are derived. ( F ) Stiffness of TissueSpec ® ECM Hydrogels. ( G ). MCF7- ESR1 Y537S cells were cultured at a density of 2 × 10 3 cells/well and embedded in 3D hydrogels to mimic different metastatic niches (bone, liver, lung) for breast cancer cells, and they were treated with different endocrine agents (4OHT [10 −6 ], Fulv [10 −6 ] and Palb [10 −6 ]) and in combination with SEL [10 −7 ]. ( H ). Colony size (pixels) and number were quantified using OpenCFU colony counting software ( http://opencfu.sourceforge.net/ ). A one-way analysis of variance (ANOVA) model was used for statistical significance of treatment and values were presented as mean ± SEM from three biological replicates (** p <0.01, *** p <0.001; **** p < 0.0001).

    Techniques Used: Expressing, RNA Sequencing Assay, Viability Assay, Electron Microscopy, Derivative Assay, Cell Culture, Software

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    • bone ecm  (Xylyx Bio)
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    Xylyx Bio bone ecm
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    tissuespec bone  (Xylyx Bio)
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    Tissuespec Bone, supplied by Xylyx Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    site metastasis kit  (Xylyx Bio)
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    Xylyx Bio site metastasis kit
    Site Metastasis Kit, supplied by Xylyx Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/site metastasis kit/product/Xylyx Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    site metastasis kit - by Bioz Stars, 2023-11
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    site tm metastasis kit  (Xylyx Bio)
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    Xylyx Bio site tm metastasis kit
    Site Tm Metastasis Kit, supplied by Xylyx Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/site tm metastasis kit/product/Xylyx Bio
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    tissuespec bone ecm hydrogel  (Xylyx Bio)
    94
    Xylyx Bio tissuespec bone ecm hydrogel
    Tissuespec Bone Ecm Hydrogel, supplied by Xylyx Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tissuespec bone ecm hydrogel/product/Xylyx Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tissuespec bone ecm hydrogel - by Bioz Stars, 2023-11
    94/100 stars
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