Journal: Developmental cell

Article Title: EGFR/Pak Signaling Selectively Regulates Glutamine Deprivation-Induced Macropinocytosis

doi: 10.1016/j.devcel.2019.05.043

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: MIA PaCa-2 (MP-2) , ATCC , ATCC CRL-1420.

Techniques: Recombinant, Fluorescence, DC Protein Assay, Stripping Membranes, Transfection, Plasmid Preparation, Negative Control, Software

Journal: BMC Molecular and Cell Biology

Article Title: PGRMC1 phosphorylation affects cell shape, motility, glycolysis, mitochondrial form and function, and tumor growth

doi: 10.1186/s12860-020-00256-3

Figure Lengend Snippet: MIA PaCa-2 pancreatic cancer cells morphology is affected by PGRMC1 phosphorylation status. a PGRMC1-HA proteins constructed for this figure. TMH: Trans-membrane helix. HA: the C-terminal 3x hemaglutinin tag. b Detection of exogenous PGRMC1 expression levels by western blot (upper panel). Equal loading is controlled by quantifying beta actin (lower panel). The results show three totally independent stably transfected cell lines per plasmid from (A). Open arrow: Exogenous PGRMC1-HA (Ex.). Shaded arrow: endogenous PGRMC1 (End.). Filled arrow: beta actin. The molecular weight ladder is Bio-Rad 1610377 Dual Xtra Standards. c Box plots quantification of replicate gels of (B) with signals normalized to beta actin from the same respective lanes. n = 4 lanes for MP and n = 6 for WT, DM and TM (replicates of respective lines 1–3 per condition). There were no significant differences (ns) except for the exogenous band in MP (ANOVA, post-hoc Dunnet’s T3). d Western blot quantification of HA-tagged exogenous PGRMC1, following B but detecting PGRMC1 with anti-HA antibody. The molecular weight ladder is Abcam ab116028 Prestained Protein Ladder. e PGRMC1 mutant protein expression alters MIA PaCa-2 cell morphology. PGRMC1-HA-expressing stable cells (respective lines 1 from B) or MP cells were stained with a FITC-tagged anti-HA antibody (Anti-HA) and imaged by confocal microscopy. DNA was stained with DAPI. Cells were also imaged in differential interference contrast (DIC) microscopy mode. The respective left panels show merged images of all 3 channels. f The rounded phenotype of double and triple mutant (E) was reversed to elongated phenotype after 125 μM ROCKI addition, but not by addition of DMSO vehicle control

Article Snippet: MIA PaCa-2 (MP) cell identity was verified as MIA PaCa-2 (ATCC CRL-1420) by the MHTP Medical Genomics Facility (Monash University, Melbourne) following the ATCC Standards Development Organization document ASN-0002 for cell line identification via short tandem repeat profiling.

Techniques: Construct, Expressing, Western Blot, Stable Transfection, Transfection, Plasmid Preparation, Molecular Weight, Mutagenesis, Staining, Confocal Microscopy, Microscopy

Journal: Nano today

Article Title: Nanoparticulate Drug Delivery Systems Targeting Inflammation for Treatment of Inflammatory Bowel Disease

doi: 10.1016/j.nantod.2017.08.006

Figure Lengend Snippet: Drugs delivered by NPs to the site of inflammation in IBD.

Article Snippet: DSS mice [ 161 ] Ovalbumin (model protein) Mannose modified PEG-PCL 212 ± 8 nm −7 ± 2 mV Ligand-receptor N.A. (Ex vivo) DSS mice [ 140 ] siRNA and Antisense oligonucleotide Antisense oligonucleotide against TNF-α Cationic konjac glucomannan (cKGM) ~200 nm +16.7 mV Ligand-receptor Oral DSS mice [ 189 ] miRNA-155 inhibitor B cell-derived exosomes 30 – 100 nm Not reported Ligand-receptor DSS mice [ 211 ] TNF-α siRNA TPP-PPM 211 – 275 nm Not reported Ligand-receptor Ex vivo DSS mice [ 153 ] CD98 siRNA CD98siRNA/ CD98 antibody modified urocanic acid/PEI NP 211 – 275 nm Not reported 147 – 261 nm +7.9 to +17.3 mV Ligand-receptor Oral DSS mice CD4 + CD4 5RB hi T cell transfer colitis [ 152 ] Cyclin D1 siRNA Liposome-based, β7 integrin-targeted NP ~ 150 nm −24 to −18 mV Ligand-receptor I.V. DDS mice [ 150 ] Antisense oligonucleotide against TNF-α Galactosylated low molecular weight chitosan Not reported Ligand-receptor Rectal TNBS mice CD4 + CD4 5RB hi T cell transfer colitis mice [ 154 ] Map4k4 siRNA Galactosylated trimethyl chitosan- cysteine/TPP NPs 140 –160 nm +20 to +42 mV Ligand-receptor Oral DSS mice [ 155 ] TNFα siRNA PLA-PEG NP bearing Fab’ portion of F4/80 antibody 376 ± 19 nm ~+ 2.56 mV Ligand-receptor Oral DSS mice [ 156 ] Map4k4 siRNA β1,3-D-glucan MP 2–4 μm Not reported Ligand-receptor Oral LPS-induced lethality [ 212 ] TNF-α siRNA Thioketal NPs 600.1 ± 281 nm 5.84 ± 0.8 mV Degradation Oral DSS mice [ 165 ] Probiotic Lyophilized probiotic bacteria (L. casei ATCC 39392) Chitosan-coated PLGA NPs 226.3 ± 0.97 nm 25.65 ± 7.02 mV Degradation Oral TNBS rat [ 172 ] Open in a separate window Drugs delivered by NPs to the site of inflammation in IBD.

Techniques: Modification, Ex Vivo, Molecular Weight