cd13 expression  (Sino Biological)


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    Sino Biological cd13 expression
    Binding of TNFα proteins to <t>CD13-expressing</t> HT1080 cells. a Expression of CD13 in HT1080 cells indicated by flow cytometry with an antibody against CD13 (α-CD13). b,c Binding of TNFα proteins to HT1080 cells. FAM-labeled TNFα proteins (100–500 nM) were incubated with HT1080 cells for 1 h at room temperature prior to flow cytometry analysis
    Cd13 Expression, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd13 expression/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd13 expression - by Bioz Stars, 2021-09
    86/100 stars

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    1) Product Images from "A single nucleotide mutation drastically increases the expression of tumor-homing NGR-TNFα in the E. coli M15-pQE30 system by improving gene transcription"

    Article Title: A single nucleotide mutation drastically increases the expression of tumor-homing NGR-TNFα in the E. coli M15-pQE30 system by improving gene transcription

    Journal: Applied Microbiology and Biotechnology

    doi: 10.1007/s00253-021-11136-x

    Binding of TNFα proteins to CD13-expressing HT1080 cells. a Expression of CD13 in HT1080 cells indicated by flow cytometry with an antibody against CD13 (α-CD13). b,c Binding of TNFα proteins to HT1080 cells. FAM-labeled TNFα proteins (100–500 nM) were incubated with HT1080 cells for 1 h at room temperature prior to flow cytometry analysis
    Figure Legend Snippet: Binding of TNFα proteins to CD13-expressing HT1080 cells. a Expression of CD13 in HT1080 cells indicated by flow cytometry with an antibody against CD13 (α-CD13). b,c Binding of TNFα proteins to HT1080 cells. FAM-labeled TNFα proteins (100–500 nM) were incubated with HT1080 cells for 1 h at room temperature prior to flow cytometry analysis

    Techniques Used: Binding Assay, Expressing, Flow Cytometry, Labeling, Incubation

    Related Articles

    Expressing:

    Article Title: Expression of PD-L1 and other immunotherapeutic targets in thymic epithelial tumors
    Article Snippet: .. CD137 expression has recently been used to identify neoantigen-specific T cells[ ] and promising data of a CD137 agonist in combination with PD-1 blockade therapy was recently presented in patients with melanoma (response rate 50% [23/46] with similar rates in patients with PD-L1 positive and negative tumors). ..

    Article Title: A human coronavirus evolves antigenically to escape antibody immunity
    Article Snippet: .. To make 293T cells infectable by 229E, we therefore transiently transfected them with an APN protein expression plasmid (SinoBiological, NM_001150.2) prior to seeding the cells for infection. ..

    Article Title: A single nucleotide mutation drastically increases the expression of tumor-homing NGR-TNFα in the E. coli M15-pQE30 system by improving gene transcription
    Article Snippet: .. The CD13 expression in HT1080 cells was measured by flow cytometry (Kessler et al. ). ..

    Article Title: A human coronavirus evolves antigenically to escape antibody immunityHigh-Throughput, Single-Copy Sequencing Reveals SARS-CoV-2 Spike Variants Coincident with Mounting Humoral Immunity during Acute COVID-19
    Article Snippet: .. Therefore, to make 293T cells infectable by 229E, we transiently transfected them with an APN protein expression plasmid (SinoBiological, NM_001150.2) prior to seeding the cells for infection. ..

    Transfection:

    Article Title: A human coronavirus evolves antigenically to escape antibody immunity
    Article Snippet: .. To make 293T cells infectable by 229E, we therefore transiently transfected them with an APN protein expression plasmid (SinoBiological, NM_001150.2) prior to seeding the cells for infection. ..

    Article Title: A human coronavirus evolves antigenically to escape antibody immunityHigh-Throughput, Single-Copy Sequencing Reveals SARS-CoV-2 Spike Variants Coincident with Mounting Humoral Immunity during Acute COVID-19
    Article Snippet: .. Therefore, to make 293T cells infectable by 229E, we transiently transfected them with an APN protein expression plasmid (SinoBiological, NM_001150.2) prior to seeding the cells for infection. ..

    Plasmid Preparation:

    Article Title: A human coronavirus evolves antigenically to escape antibody immunity
    Article Snippet: .. To make 293T cells infectable by 229E, we therefore transiently transfected them with an APN protein expression plasmid (SinoBiological, NM_001150.2) prior to seeding the cells for infection. ..

    Article Title: A human coronavirus evolves antigenically to escape antibody immunityHigh-Throughput, Single-Copy Sequencing Reveals SARS-CoV-2 Spike Variants Coincident with Mounting Humoral Immunity during Acute COVID-19
    Article Snippet: .. Therefore, to make 293T cells infectable by 229E, we transiently transfected them with an APN protein expression plasmid (SinoBiological, NM_001150.2) prior to seeding the cells for infection. ..

    Infection:

    Article Title: A human coronavirus evolves antigenically to escape antibody immunity
    Article Snippet: .. To make 293T cells infectable by 229E, we therefore transiently transfected them with an APN protein expression plasmid (SinoBiological, NM_001150.2) prior to seeding the cells for infection. ..

    Article Title: A human coronavirus evolves antigenically to escape antibody immunityHigh-Throughput, Single-Copy Sequencing Reveals SARS-CoV-2 Spike Variants Coincident with Mounting Humoral Immunity during Acute COVID-19
    Article Snippet: .. Therefore, to make 293T cells infectable by 229E, we transiently transfected them with an APN protein expression plasmid (SinoBiological, NM_001150.2) prior to seeding the cells for infection. ..

    Recombinant:

    Article Title: The pro-inflammatory cytokine 14-3-3ε is a ligand of CD13 in cartilage
    Article Snippet: .. Human recombinant CD13 (Sino Biological) was immobilized to a CM5 sensor chip (GE Healthcare) at 11,949 resonance units (RU) by amine coupling in 10 mM sodium acetate (pH 5.0) according to the manufacturer's instructions. ..

    Article Title: NGR-tagged nano-gold: A new CD13-selective carrier for cytokine delivery to tumors
    Article Snippet: .. For the CD13 enzymatic activity assay, 96-well PVC microtiter plates were filled with a mixture of recombinant human His-tagged CD13 (0.2 µg/mL, Sino Biological Inc., Beijing, China), L-alanine-p-nitroanilide (0.5 mM; Sigma) and various amounts of peptide in 60 mM potassium phosphate buffer, pH 7.4 (100 µL/well, final volume). ..

    Chromatin Immunoprecipitation:

    Article Title: The pro-inflammatory cytokine 14-3-3ε is a ligand of CD13 in cartilage
    Article Snippet: .. Human recombinant CD13 (Sino Biological) was immobilized to a CM5 sensor chip (GE Healthcare) at 11,949 resonance units (RU) by amine coupling in 10 mM sodium acetate (pH 5.0) according to the manufacturer's instructions. ..

    Enzyme Activity Assay:

    Article Title: NGR-tagged nano-gold: A new CD13-selective carrier for cytokine delivery to tumors
    Article Snippet: .. For the CD13 enzymatic activity assay, 96-well PVC microtiter plates were filled with a mixture of recombinant human His-tagged CD13 (0.2 µg/mL, Sino Biological Inc., Beijing, China), L-alanine-p-nitroanilide (0.5 mM; Sigma) and various amounts of peptide in 60 mM potassium phosphate buffer, pH 7.4 (100 µL/well, final volume). ..

    Flow Cytometry:

    Article Title: A single nucleotide mutation drastically increases the expression of tumor-homing NGR-TNFα in the E. coli M15-pQE30 system by improving gene transcription
    Article Snippet: .. The CD13 expression in HT1080 cells was measured by flow cytometry (Kessler et al. ). ..

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  • 86
    Sino Biological cd13 expression
    Binding of TNFα proteins to <t>CD13-expressing</t> HT1080 cells. a Expression of CD13 in HT1080 cells indicated by flow cytometry with an antibody against CD13 (α-CD13). b,c Binding of TNFα proteins to HT1080 cells. FAM-labeled TNFα proteins (100–500 nM) were incubated with HT1080 cells for 1 h at room temperature prior to flow cytometry analysis
    Cd13 Expression, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd13 expression/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd13 expression - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

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    Binding of TNFα proteins to CD13-expressing HT1080 cells. a Expression of CD13 in HT1080 cells indicated by flow cytometry with an antibody against CD13 (α-CD13). b,c Binding of TNFα proteins to HT1080 cells. FAM-labeled TNFα proteins (100–500 nM) were incubated with HT1080 cells for 1 h at room temperature prior to flow cytometry analysis

    Journal: Applied Microbiology and Biotechnology

    Article Title: A single nucleotide mutation drastically increases the expression of tumor-homing NGR-TNFα in the E. coli M15-pQE30 system by improving gene transcription

    doi: 10.1007/s00253-021-11136-x

    Figure Lengend Snippet: Binding of TNFα proteins to CD13-expressing HT1080 cells. a Expression of CD13 in HT1080 cells indicated by flow cytometry with an antibody against CD13 (α-CD13). b,c Binding of TNFα proteins to HT1080 cells. FAM-labeled TNFα proteins (100–500 nM) were incubated with HT1080 cells for 1 h at room temperature prior to flow cytometry analysis

    Article Snippet: The CD13 expression in HT1080 cells was measured by flow cytometry (Kessler et al. ).

    Techniques: Binding Assay, Expressing, Flow Cytometry, Labeling, Incubation