dynamin 1 wildtype wt  (ATCC)

Journal: Pathogens

Article Title: Development of an Indirect ELISA to Detect Equine Antibodies to Theileria haneyi

doi: 10.3390/pathogens10030270

Figure Lengend Snippet: Cloning and expression of T. haneyi EMA11 ( Th EMA11). ( a ) Codon-optimized sequence of Th EMA11 was cloned into pcDNA3.1 in frame with the CMV promoter and fused with the 6His tag originating the plasmid pcDNA3.1- Th EMA11. ( b ) Expression of recombinant Th EMA11 in HEK 293t cells detected by anti-6× His monoclonal antibody.

Article Snippet: The synthetic gene was then cloned into pcDNA3.1 and the plasmid, termed pcDNA3.1- Th EMA11, was then used to express the target protein in human embryonic kidney (HEK) 293t cells (ATCC ® , Gaithersburg, MD, USA).

Techniques: Clone Assay, Expressing, Sequencing, Plasmid Preparation, Recombinant

Journal: Pathogens

Article Title: Development of an Indirect ELISA to Detect Equine Antibodies to Theileria haneyi

doi: 10.3390/pathogens10030270

Figure Lengend Snippet: Immunoblot analyses to evaluate the antigenicity of recombinant Th EMA11 using sera from horses experimentally infected with T. haneyi ( a ), sera from uninfected horses ( b ), and sera from T. equi infected horses ( c ). HEK 293t cells expressing GFP were used as a negative control. Blots from one representative animal in each group are shown.

Article Snippet: The synthetic gene was then cloned into pcDNA3.1 and the plasmid, termed pcDNA3.1- Th EMA11, was then used to express the target protein in human embryonic kidney (HEK) 293t cells (ATCC ® , Gaithersburg, MD, USA).

Techniques: Western Blot, Recombinant, Infection, Expressing, Negative Control

Journal: Pathogens

Article Title: Development of an Indirect ELISA to Detect Equine Antibodies to Theileria haneyi

doi: 10.3390/pathogens10030270

Figure Lengend Snippet: Antigenicity of purified recombinant Th EMA11 using sera from T. haneyi -infected horses (Ho-344 and Ho-777). Sera from uninfected horses (Ho-395 and Ho-404) were used as negative controls. Rabbit anti-horse IgG-HRP alone was also used as a negative control.

Article Snippet: The synthetic gene was then cloned into pcDNA3.1 and the plasmid, termed pcDNA3.1- Th EMA11, was then used to express the target protein in human embryonic kidney (HEK) 293t cells (ATCC ® , Gaithersburg, MD, USA).

Techniques: Purification, Recombinant, Infection, Negative Control

Journal: Pathogens

Article Title: Development of an Indirect ELISA to Detect Equine Antibodies to Theileria haneyi

doi: 10.3390/pathogens10030270

Figure Lengend Snippet: Titration of the optimal amount of recombinant Th EMA11 in an ELISA format in relation to horse serum dilutions. Results show titration of serum from one representative T. haneyi -infected horse and serum from one representative uninfected horse. Four serial dilutions of the sera (1:8, 1:16, 1:32, and 1:64), and four different Th EMA11 recombinant protein concentrations (5 μL/well, 4 μL/well, 2 μL/well, and 1 μL/well) were tested ( a–d ). Error bars represent the standard deviation of technical replicates.

Article Snippet: The synthetic gene was then cloned into pcDNA3.1 and the plasmid, termed pcDNA3.1- Th EMA11, was then used to express the target protein in human embryonic kidney (HEK) 293t cells (ATCC ® , Gaithersburg, MD, USA).

Techniques: Titration, Recombinant, Enzyme-linked Immunosorbent Assay, Infection, Standard Deviation

Journal: Pathogens

Article Title: Development of an Indirect ELISA to Detect Equine Antibodies to Theileria haneyi

doi: 10.3390/pathogens10030270

Figure Lengend Snippet: Results of the Th EMA11-based ELISA using serum samples from T. haneyi experimentally infected horses (n = 18), from T. equi experimentally infected horses (n = 9), and from uninfected horses (n = 19). The dashed line indicates the cutoff of 0.8 OD450 nm, representing the average of negative samples (uninfected horse sera) plus three standard deviations. The solid lines represent the average OD for each group.

Article Snippet: The synthetic gene was then cloned into pcDNA3.1 and the plasmid, termed pcDNA3.1- Th EMA11, was then used to express the target protein in human embryonic kidney (HEK) 293t cells (ATCC ® , Gaithersburg, MD, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Infection

Journal: Pathogens

Article Title: Development of an Indirect ELISA to Detect Equine Antibodies to Theileria haneyi

doi: 10.3390/pathogens10030270

Figure Lengend Snippet: Use of the Th EMA11-based ELISA to investigate the presence of antibodies against T. haneyi in serum samples (n = 176) from horses residing in or with a travel history to distinct geographical regions endemic for equine theileriosis. The dashed line indicates the cutoff of 0.8 OD450 nm, previously determined representing the average of negative samples (uninfected horse sera) plus three standard deviations. The solid lines represent the average OD for each group. 100/176 equine field serum samples were positive for T. haneyi , and 76/176 were negative.

Article Snippet: The synthetic gene was then cloned into pcDNA3.1 and the plasmid, termed pcDNA3.1- Th EMA11, was then used to express the target protein in human embryonic kidney (HEK) 293t cells (ATCC ® , Gaithersburg, MD, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Pathogens

Article Title: Development of an Indirect ELISA to Detect Equine Antibodies to Theileria haneyi

doi: 10.3390/pathogens10030270

Figure Lengend Snippet: Presence of T. haneyi EMA11 and T. equi EMA1 antibodies in horse sera from distinct geographic regions.

Article Snippet: The synthetic gene was then cloned into pcDNA3.1 and the plasmid, termed pcDNA3.1- Th EMA11, was then used to express the target protein in human embryonic kidney (HEK) 293t cells (ATCC ® , Gaithersburg, MD, USA).

Techniques:

Journal: Pathogens

Article Title: Development of an Indirect ELISA to Detect Equine Antibodies to Theileria haneyi

doi: 10.3390/pathogens10030270

Figure Lengend Snippet: Presence of T. haneyi EMA11 and B. caballi RAP-1 antibodies in horse sera from distinct geographic regions.

Article Snippet: The synthetic gene was then cloned into pcDNA3.1 and the plasmid, termed pcDNA3.1- Th EMA11, was then used to express the target protein in human embryonic kidney (HEK) 293t cells (ATCC ® , Gaithersburg, MD, USA).

Techniques:

Journal: Pathogens

Article Title: Development of an Indirect ELISA to Detect Equine Antibodies to Theileria haneyi

doi: 10.3390/pathogens10030270

Figure Lengend Snippet: Cloning and expression of T. haneyi EMA11 ( Th EMA11). ( a ) Codon-optimized sequence of Th EMA11 was cloned into pcDNA3.1 in frame with the CMV promoter and fused with the 6His tag originating the plasmid pcDNA3.1- Th EMA11. ( b ) Expression of recombinant Th EMA11 in HEK 293t cells detected by anti-6× His monoclonal antibody.

Article Snippet: The synthetic gene was then cloned into pcDNA3.1 and the plasmid, termed pcDNA3.1- Th EMA11, was then used to express the target protein in human embryonic kidney (HEK) 293t cells (ATCC ® , Gaithersburg, MD, USA).

Techniques: Clone Assay, Expressing, Sequencing, Plasmid Preparation, Recombinant