α tubulin  (Boster Bio)


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    Boster Bio α tubulin
    Effects of inhibition of Hsp70 expression in cardiomyocytes on cell injury induced by GC treatment. a Inhibition of HSP70 expression in cardiomyocytes with KNK437. Then HSP70 content in cardiomyocytes was detected by western blot method. Alpha <t>(α)-tubulin</t>
    α Tubulin, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α tubulin/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α tubulin - by Bioz Stars, 2022-05
    93/100 stars

    Images

    1) Product Images from "Effects of HIP in protection of HSP70 for stress-induced cardiomyocytes injury and its glucorticoid receptor pathway"

    Article Title: Effects of HIP in protection of HSP70 for stress-induced cardiomyocytes injury and its glucorticoid receptor pathway

    Journal: Cell Stress & Chaperones

    doi: 10.1007/s12192-014-0510-y

    Effects of inhibition of Hsp70 expression in cardiomyocytes on cell injury induced by GC treatment. a Inhibition of HSP70 expression in cardiomyocytes with KNK437. Then HSP70 content in cardiomyocytes was detected by western blot method. Alpha (α)-tubulin
    Figure Legend Snippet: Effects of inhibition of Hsp70 expression in cardiomyocytes on cell injury induced by GC treatment. a Inhibition of HSP70 expression in cardiomyocytes with KNK437. Then HSP70 content in cardiomyocytes was detected by western blot method. Alpha (α)-tubulin

    Techniques Used: Inhibition, Expressing, Western Blot

    2) Product Images from "Inflammasome-Induced Osmotic Pressure and the Mechanical Mechanisms Underlying Astrocytic Swelling and Membrane Blebbing in Pyroptosis"

    Article Title: Inflammasome-Induced Osmotic Pressure and the Mechanical Mechanisms Underlying Astrocytic Swelling and Membrane Blebbing in Pyroptosis

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.688674

    ASC-induced inflammasome assembly regulated PN-OP. (A) RT-qPCR was used to measure the relative transcript level of ASC after transient transfection with the ASC siRNA. (B) The cytoplasmic OP values of U87 cells were measured using a freezing point osmometer under co-treatment of 4-OHT with the ASC siRNA. (C) The count rate of PNs. (D) Caspase-1 activity of cells. (E) Representative images and mean values of normalized CFP/FRET ratios of Vimentin tension under the different treatments. Arrows indicate the membrane blebbing regions. The calibration bar was set from 0.1 to 2.0. Scale bar, 10 μm. (F) Calcium imaging micrographs and traces of relative Fluo-4 fluorescence intensity (Ft/F0) of cells. (G) Chloridion imaging micrographs and traces of relative MQAE fluorescence intensity (Ft/F0) of cells. The increase of intracellular Cl - levels led to the decrease of MQAE fluorescence value instead. (H) Monolayers of U87 cells were subjected to 4-OHT treatment alone, or co-treatment with 4-OHT and the ASC siRNA, then stained with β-actin (FITC) or α-tubulin (TRITC). The images were generated from the fluorescence inverted microscope after immunofluorescence staining. Mean of ≥ 3 experiments ± SEM. Values marked with asterisks are significantly different, as determined using a t -test.
    Figure Legend Snippet: ASC-induced inflammasome assembly regulated PN-OP. (A) RT-qPCR was used to measure the relative transcript level of ASC after transient transfection with the ASC siRNA. (B) The cytoplasmic OP values of U87 cells were measured using a freezing point osmometer under co-treatment of 4-OHT with the ASC siRNA. (C) The count rate of PNs. (D) Caspase-1 activity of cells. (E) Representative images and mean values of normalized CFP/FRET ratios of Vimentin tension under the different treatments. Arrows indicate the membrane blebbing regions. The calibration bar was set from 0.1 to 2.0. Scale bar, 10 μm. (F) Calcium imaging micrographs and traces of relative Fluo-4 fluorescence intensity (Ft/F0) of cells. (G) Chloridion imaging micrographs and traces of relative MQAE fluorescence intensity (Ft/F0) of cells. The increase of intracellular Cl - levels led to the decrease of MQAE fluorescence value instead. (H) Monolayers of U87 cells were subjected to 4-OHT treatment alone, or co-treatment with 4-OHT and the ASC siRNA, then stained with β-actin (FITC) or α-tubulin (TRITC). The images were generated from the fluorescence inverted microscope after immunofluorescence staining. Mean of ≥ 3 experiments ± SEM. Values marked with asterisks are significantly different, as determined using a t -test.

    Techniques Used: Quantitative RT-PCR, Transfection, Activity Assay, Imaging, Fluorescence, Staining, Generated, Inverted Microscopy, Immunofluorescence

    Caspase-1 inhibitor decreased PN-OP and prevented pyroptosis. (A) RT-qPCR was used to measure the relative transcript level of CASPASE-1 after transient transfection with the CASPASE-1 siRNA. (B) Z‑VAD-FMK (20 μM) treatment or CASPASE-1 siRNA transfection of U87 cells inhibited the caspase-1 activity. (C) IHC analysis of caspase-1 expression in brain section of the sepsis mouse models. The cytoplasm was stained with the caspase-1 antibody (brown). Semiquantitative analysis of the IHC staining by comparison of AOD among the groups. (D) The cytoplasmic OP values of U87 cells were measured using a freezing point osmometer. (E) The count rate of PNs in U87 cells. (F) Nano size distribution value of protein granules in the cytoplasm. Black dash line represents value of the 50-100 nm peak in the 4-OHT group. (G) Representative images and mean values of normalized CFP/FRET ratios of Vimentin tension. Arrows indicate the membrane blebbing regions. (H) Calcium imaging micrographs and traces of relative Fluo-4 fluorescence intensity (Ft/F0) of cells. (I) Chloridion imaging micrographs and traces of relative MQAE fluorescence intensity (Ft/F0) of cells. (J, K) Monolayers of U87 cells were stained for β-actin (FITC), α-tubulin (TRITC). (L) Immunofluorescence of ASC and NLRP3 in U87 cells using antibodies. The images were generated from the fluorescence inverted microscope after immunofluorescence staining. Mean of ≥ 3 experiments ± SEM. Values marked with asterisks are significantly different, as determined using a t -test.
    Figure Legend Snippet: Caspase-1 inhibitor decreased PN-OP and prevented pyroptosis. (A) RT-qPCR was used to measure the relative transcript level of CASPASE-1 after transient transfection with the CASPASE-1 siRNA. (B) Z‑VAD-FMK (20 μM) treatment or CASPASE-1 siRNA transfection of U87 cells inhibited the caspase-1 activity. (C) IHC analysis of caspase-1 expression in brain section of the sepsis mouse models. The cytoplasm was stained with the caspase-1 antibody (brown). Semiquantitative analysis of the IHC staining by comparison of AOD among the groups. (D) The cytoplasmic OP values of U87 cells were measured using a freezing point osmometer. (E) The count rate of PNs in U87 cells. (F) Nano size distribution value of protein granules in the cytoplasm. Black dash line represents value of the 50-100 nm peak in the 4-OHT group. (G) Representative images and mean values of normalized CFP/FRET ratios of Vimentin tension. Arrows indicate the membrane blebbing regions. (H) Calcium imaging micrographs and traces of relative Fluo-4 fluorescence intensity (Ft/F0) of cells. (I) Chloridion imaging micrographs and traces of relative MQAE fluorescence intensity (Ft/F0) of cells. (J, K) Monolayers of U87 cells were stained for β-actin (FITC), α-tubulin (TRITC). (L) Immunofluorescence of ASC and NLRP3 in U87 cells using antibodies. The images were generated from the fluorescence inverted microscope after immunofluorescence staining. Mean of ≥ 3 experiments ± SEM. Values marked with asterisks are significantly different, as determined using a t -test.

    Techniques Used: Quantitative RT-PCR, Transfection, Activity Assay, Immunohistochemistry, Expressing, Staining, Imaging, Fluorescence, Immunofluorescence, Generated, Inverted Microscopy

    Calcium ions released from the endoplasmic reticulum regulated pyroptosis. (A) The cytoplasmic OP values of U87 cells were measured using a freezing point osmometer under the isotonic control, 4-OHT treatment, and co-treatments of 4-OHT with caffeine (2 mM), 2-APB (100 μM), and dantrolene (20 μM). (B) The count rate of PNs in U87 cells. (C) Representative images and mean values of normalized CFP/FRET ratios of Vimentin tension under the different treatments. Arrows indicate the membrane blebbing regions. (D) Calcium imaging micrographs and traces of relative Fluo-4 fluorescence intensity (Ft/F0) of cells. (E) Chloridion imaging micrographs and traces of relative MQAE fluorescence intensity (Ft/F0) of cells. (F) Representative time‑lapse images of U87 cells subjected to different treatments. Cell membrane integrity was monitored by PI uptake (red fluorescence). The bar charts represent percentage of cells with PI positivity and membrane blebbing. (G) LDH release and (H) caspase-1 activity of cells under the different treatments. (I) The control, 4-OHT, -caffeine, -2-APB, -dantrolene-treated U87 cell monolayers were stained for β-actin (FITC), α-tubulin (TRITC). The images were generated from the fluorescence inverted microscope after immunofluorescence staining. (J) IHC analysis of caspase-1 expression in brain section of the sepsis mouse models. The cytoplasm was stained with the caspase-1 antibody (brown). Semiquantitative analysis of the IHC staining by AOD among the groups. Mean of ≥ 3 experiments ± SEM. Values marked with asterisks are significantly different, as determined using a t -test.
    Figure Legend Snippet: Calcium ions released from the endoplasmic reticulum regulated pyroptosis. (A) The cytoplasmic OP values of U87 cells were measured using a freezing point osmometer under the isotonic control, 4-OHT treatment, and co-treatments of 4-OHT with caffeine (2 mM), 2-APB (100 μM), and dantrolene (20 μM). (B) The count rate of PNs in U87 cells. (C) Representative images and mean values of normalized CFP/FRET ratios of Vimentin tension under the different treatments. Arrows indicate the membrane blebbing regions. (D) Calcium imaging micrographs and traces of relative Fluo-4 fluorescence intensity (Ft/F0) of cells. (E) Chloridion imaging micrographs and traces of relative MQAE fluorescence intensity (Ft/F0) of cells. (F) Representative time‑lapse images of U87 cells subjected to different treatments. Cell membrane integrity was monitored by PI uptake (red fluorescence). The bar charts represent percentage of cells with PI positivity and membrane blebbing. (G) LDH release and (H) caspase-1 activity of cells under the different treatments. (I) The control, 4-OHT, -caffeine, -2-APB, -dantrolene-treated U87 cell monolayers were stained for β-actin (FITC), α-tubulin (TRITC). The images were generated from the fluorescence inverted microscope after immunofluorescence staining. (J) IHC analysis of caspase-1 expression in brain section of the sepsis mouse models. The cytoplasm was stained with the caspase-1 antibody (brown). Semiquantitative analysis of the IHC staining by AOD among the groups. Mean of ≥ 3 experiments ± SEM. Values marked with asterisks are significantly different, as determined using a t -test.

    Techniques Used: Imaging, Fluorescence, Activity Assay, Staining, Generated, Inverted Microscopy, Immunofluorescence, Immunohistochemistry, Expressing

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    Boster Bio α tubulin
    Effects of inhibition of Hsp70 expression in cardiomyocytes on cell injury induced by GC treatment. a Inhibition of HSP70 expression in cardiomyocytes with KNK437. Then HSP70 content in cardiomyocytes was detected by western blot method. Alpha <t>(α)-tubulin</t>
    α Tubulin, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α tubulin/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α tubulin - by Bioz Stars, 2022-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    Effects of inhibition of Hsp70 expression in cardiomyocytes on cell injury induced by GC treatment. a Inhibition of HSP70 expression in cardiomyocytes with KNK437. Then HSP70 content in cardiomyocytes was detected by western blot method. Alpha (α)-tubulin

    Journal: Cell Stress & Chaperones

    Article Title: Effects of HIP in protection of HSP70 for stress-induced cardiomyocytes injury and its glucorticoid receptor pathway

    doi: 10.1007/s12192-014-0510-y

    Figure Lengend Snippet: Effects of inhibition of Hsp70 expression in cardiomyocytes on cell injury induced by GC treatment. a Inhibition of HSP70 expression in cardiomyocytes with KNK437. Then HSP70 content in cardiomyocytes was detected by western blot method. Alpha (α)-tubulin

    Article Snippet: The membranes were blocked in TBS buffer (25 mmol/L Tris–HCl, 150 mmol/L NaCl, pH 7.5) containing 0.05 % Tween-20 and 5 % milk and incubated with a mouse monoclonal antibody against rat HIP (R-19, Santa Cruz), α-Tubulin (BM1452, Boster, china), HSP70 (SC-1060, Santa Cruz) in blocking solution.

    Techniques: Inhibition, Expressing, Western Blot

    ASC-induced inflammasome assembly regulated PN-OP. (A) RT-qPCR was used to measure the relative transcript level of ASC after transient transfection with the ASC siRNA. (B) The cytoplasmic OP values of U87 cells were measured using a freezing point osmometer under co-treatment of 4-OHT with the ASC siRNA. (C) The count rate of PNs. (D) Caspase-1 activity of cells. (E) Representative images and mean values of normalized CFP/FRET ratios of Vimentin tension under the different treatments. Arrows indicate the membrane blebbing regions. The calibration bar was set from 0.1 to 2.0. Scale bar, 10 μm. (F) Calcium imaging micrographs and traces of relative Fluo-4 fluorescence intensity (Ft/F0) of cells. (G) Chloridion imaging micrographs and traces of relative MQAE fluorescence intensity (Ft/F0) of cells. The increase of intracellular Cl - levels led to the decrease of MQAE fluorescence value instead. (H) Monolayers of U87 cells were subjected to 4-OHT treatment alone, or co-treatment with 4-OHT and the ASC siRNA, then stained with β-actin (FITC) or α-tubulin (TRITC). The images were generated from the fluorescence inverted microscope after immunofluorescence staining. Mean of ≥ 3 experiments ± SEM. Values marked with asterisks are significantly different, as determined using a t -test.

    Journal: Frontiers in Immunology

    Article Title: Inflammasome-Induced Osmotic Pressure and the Mechanical Mechanisms Underlying Astrocytic Swelling and Membrane Blebbing in Pyroptosis

    doi: 10.3389/fimmu.2021.688674

    Figure Lengend Snippet: ASC-induced inflammasome assembly regulated PN-OP. (A) RT-qPCR was used to measure the relative transcript level of ASC after transient transfection with the ASC siRNA. (B) The cytoplasmic OP values of U87 cells were measured using a freezing point osmometer under co-treatment of 4-OHT with the ASC siRNA. (C) The count rate of PNs. (D) Caspase-1 activity of cells. (E) Representative images and mean values of normalized CFP/FRET ratios of Vimentin tension under the different treatments. Arrows indicate the membrane blebbing regions. The calibration bar was set from 0.1 to 2.0. Scale bar, 10 μm. (F) Calcium imaging micrographs and traces of relative Fluo-4 fluorescence intensity (Ft/F0) of cells. (G) Chloridion imaging micrographs and traces of relative MQAE fluorescence intensity (Ft/F0) of cells. The increase of intracellular Cl - levels led to the decrease of MQAE fluorescence value instead. (H) Monolayers of U87 cells were subjected to 4-OHT treatment alone, or co-treatment with 4-OHT and the ASC siRNA, then stained with β-actin (FITC) or α-tubulin (TRITC). The images were generated from the fluorescence inverted microscope after immunofluorescence staining. Mean of ≥ 3 experiments ± SEM. Values marked with asterisks are significantly different, as determined using a t -test.

    Article Snippet: Mouse anti-α-tubulin antibody was from Boster (BM1452, Wuhan, China).

    Techniques: Quantitative RT-PCR, Transfection, Activity Assay, Imaging, Fluorescence, Staining, Generated, Inverted Microscopy, Immunofluorescence

    Caspase-1 inhibitor decreased PN-OP and prevented pyroptosis. (A) RT-qPCR was used to measure the relative transcript level of CASPASE-1 after transient transfection with the CASPASE-1 siRNA. (B) Z‑VAD-FMK (20 μM) treatment or CASPASE-1 siRNA transfection of U87 cells inhibited the caspase-1 activity. (C) IHC analysis of caspase-1 expression in brain section of the sepsis mouse models. The cytoplasm was stained with the caspase-1 antibody (brown). Semiquantitative analysis of the IHC staining by comparison of AOD among the groups. (D) The cytoplasmic OP values of U87 cells were measured using a freezing point osmometer. (E) The count rate of PNs in U87 cells. (F) Nano size distribution value of protein granules in the cytoplasm. Black dash line represents value of the 50-100 nm peak in the 4-OHT group. (G) Representative images and mean values of normalized CFP/FRET ratios of Vimentin tension. Arrows indicate the membrane blebbing regions. (H) Calcium imaging micrographs and traces of relative Fluo-4 fluorescence intensity (Ft/F0) of cells. (I) Chloridion imaging micrographs and traces of relative MQAE fluorescence intensity (Ft/F0) of cells. (J, K) Monolayers of U87 cells were stained for β-actin (FITC), α-tubulin (TRITC). (L) Immunofluorescence of ASC and NLRP3 in U87 cells using antibodies. The images were generated from the fluorescence inverted microscope after immunofluorescence staining. Mean of ≥ 3 experiments ± SEM. Values marked with asterisks are significantly different, as determined using a t -test.

    Journal: Frontiers in Immunology

    Article Title: Inflammasome-Induced Osmotic Pressure and the Mechanical Mechanisms Underlying Astrocytic Swelling and Membrane Blebbing in Pyroptosis

    doi: 10.3389/fimmu.2021.688674

    Figure Lengend Snippet: Caspase-1 inhibitor decreased PN-OP and prevented pyroptosis. (A) RT-qPCR was used to measure the relative transcript level of CASPASE-1 after transient transfection with the CASPASE-1 siRNA. (B) Z‑VAD-FMK (20 μM) treatment or CASPASE-1 siRNA transfection of U87 cells inhibited the caspase-1 activity. (C) IHC analysis of caspase-1 expression in brain section of the sepsis mouse models. The cytoplasm was stained with the caspase-1 antibody (brown). Semiquantitative analysis of the IHC staining by comparison of AOD among the groups. (D) The cytoplasmic OP values of U87 cells were measured using a freezing point osmometer. (E) The count rate of PNs in U87 cells. (F) Nano size distribution value of protein granules in the cytoplasm. Black dash line represents value of the 50-100 nm peak in the 4-OHT group. (G) Representative images and mean values of normalized CFP/FRET ratios of Vimentin tension. Arrows indicate the membrane blebbing regions. (H) Calcium imaging micrographs and traces of relative Fluo-4 fluorescence intensity (Ft/F0) of cells. (I) Chloridion imaging micrographs and traces of relative MQAE fluorescence intensity (Ft/F0) of cells. (J, K) Monolayers of U87 cells were stained for β-actin (FITC), α-tubulin (TRITC). (L) Immunofluorescence of ASC and NLRP3 in U87 cells using antibodies. The images were generated from the fluorescence inverted microscope after immunofluorescence staining. Mean of ≥ 3 experiments ± SEM. Values marked with asterisks are significantly different, as determined using a t -test.

    Article Snippet: Mouse anti-α-tubulin antibody was from Boster (BM1452, Wuhan, China).

    Techniques: Quantitative RT-PCR, Transfection, Activity Assay, Immunohistochemistry, Expressing, Staining, Imaging, Fluorescence, Immunofluorescence, Generated, Inverted Microscopy

    Calcium ions released from the endoplasmic reticulum regulated pyroptosis. (A) The cytoplasmic OP values of U87 cells were measured using a freezing point osmometer under the isotonic control, 4-OHT treatment, and co-treatments of 4-OHT with caffeine (2 mM), 2-APB (100 μM), and dantrolene (20 μM). (B) The count rate of PNs in U87 cells. (C) Representative images and mean values of normalized CFP/FRET ratios of Vimentin tension under the different treatments. Arrows indicate the membrane blebbing regions. (D) Calcium imaging micrographs and traces of relative Fluo-4 fluorescence intensity (Ft/F0) of cells. (E) Chloridion imaging micrographs and traces of relative MQAE fluorescence intensity (Ft/F0) of cells. (F) Representative time‑lapse images of U87 cells subjected to different treatments. Cell membrane integrity was monitored by PI uptake (red fluorescence). The bar charts represent percentage of cells with PI positivity and membrane blebbing. (G) LDH release and (H) caspase-1 activity of cells under the different treatments. (I) The control, 4-OHT, -caffeine, -2-APB, -dantrolene-treated U87 cell monolayers were stained for β-actin (FITC), α-tubulin (TRITC). The images were generated from the fluorescence inverted microscope after immunofluorescence staining. (J) IHC analysis of caspase-1 expression in brain section of the sepsis mouse models. The cytoplasm was stained with the caspase-1 antibody (brown). Semiquantitative analysis of the IHC staining by AOD among the groups. Mean of ≥ 3 experiments ± SEM. Values marked with asterisks are significantly different, as determined using a t -test.

    Journal: Frontiers in Immunology

    Article Title: Inflammasome-Induced Osmotic Pressure and the Mechanical Mechanisms Underlying Astrocytic Swelling and Membrane Blebbing in Pyroptosis

    doi: 10.3389/fimmu.2021.688674

    Figure Lengend Snippet: Calcium ions released from the endoplasmic reticulum regulated pyroptosis. (A) The cytoplasmic OP values of U87 cells were measured using a freezing point osmometer under the isotonic control, 4-OHT treatment, and co-treatments of 4-OHT with caffeine (2 mM), 2-APB (100 μM), and dantrolene (20 μM). (B) The count rate of PNs in U87 cells. (C) Representative images and mean values of normalized CFP/FRET ratios of Vimentin tension under the different treatments. Arrows indicate the membrane blebbing regions. (D) Calcium imaging micrographs and traces of relative Fluo-4 fluorescence intensity (Ft/F0) of cells. (E) Chloridion imaging micrographs and traces of relative MQAE fluorescence intensity (Ft/F0) of cells. (F) Representative time‑lapse images of U87 cells subjected to different treatments. Cell membrane integrity was monitored by PI uptake (red fluorescence). The bar charts represent percentage of cells with PI positivity and membrane blebbing. (G) LDH release and (H) caspase-1 activity of cells under the different treatments. (I) The control, 4-OHT, -caffeine, -2-APB, -dantrolene-treated U87 cell monolayers were stained for β-actin (FITC), α-tubulin (TRITC). The images were generated from the fluorescence inverted microscope after immunofluorescence staining. (J) IHC analysis of caspase-1 expression in brain section of the sepsis mouse models. The cytoplasm was stained with the caspase-1 antibody (brown). Semiquantitative analysis of the IHC staining by AOD among the groups. Mean of ≥ 3 experiments ± SEM. Values marked with asterisks are significantly different, as determined using a t -test.

    Article Snippet: Mouse anti-α-tubulin antibody was from Boster (BM1452, Wuhan, China).

    Techniques: Imaging, Fluorescence, Activity Assay, Staining, Generated, Inverted Microscopy, Immunofluorescence, Immunohistochemistry, Expressing