calbindin  (Boster Bio)


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    • 92
    Name:
    Anti Calbindin D Calb1 Antibody
    Description:

    Catalog Number:
    MA1007
    Price:
    99.0
    Category:
    Primary Antibodies
    Reactivity:
    Bovine Human Mouse Rat
    Applications:
    IF, IHC-P, WB
    Immunogen:
    Bovine kidney calbindin-D
    Host:
    Mouse
    Isotype:
    Mouse IgG1
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    Structured Review

    Boster Bio calbindin
    Anti Calbindin D Calb1 Antibody

    https://www.bioz.com/result/calbindin/product/Boster Bio
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calbindin - by Bioz Stars, 2021-06
    92/100 stars

    Images

    1) Product Images from "Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration"

    Article Title: Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration

    Journal: Cell reports

    doi: 10.1016/j.celrep.2019.09.012

    Dendritic SNPH Intrusion in PCs of Dysmyelinating Shi Mice In Vivo (A–F) Immunohistochemistry technique for capturing SNPH intrusion in PC dendrites. Shown is SNPH (green), Syt2 (red), and Calbindin (blue) labeling in 3.5-month-old WT (A, C, and E) and Shi (B, D, and F) mice. Scale bar, 10 μm. (G and H) High magnification of the maximum intensity projection image from the z stack through dendritic regions of WT (G) and Shi (H). (I and J) Orthogonal (slice) view of SNPH punctum (indicated by arrows in panels G and H) in the dendritic region from WT (I) and Shi (J) in x-z and y-z orientations, respectively. (K) Quantification of percentage area occupied by SNPH within the dendritic volume from 3 mice of each group. Data are shown as mean ± SEM. *p
    Figure Legend Snippet: Dendritic SNPH Intrusion in PCs of Dysmyelinating Shi Mice In Vivo (A–F) Immunohistochemistry technique for capturing SNPH intrusion in PC dendrites. Shown is SNPH (green), Syt2 (red), and Calbindin (blue) labeling in 3.5-month-old WT (A, C, and E) and Shi (B, D, and F) mice. Scale bar, 10 μm. (G and H) High magnification of the maximum intensity projection image from the z stack through dendritic regions of WT (G) and Shi (H). (I and J) Orthogonal (slice) view of SNPH punctum (indicated by arrows in panels G and H) in the dendritic region from WT (I) and Shi (J) in x-z and y-z orientations, respectively. (K) Quantification of percentage area occupied by SNPH within the dendritic volume from 3 mice of each group. Data are shown as mean ± SEM. *p

    Techniques Used: Mouse Assay, In Vivo, Immunohistochemistry, Labeling

    SNPH Intrusion Sensitizes PC Dendrites to Glutamatergic Excitotoxicity In Vivo (A and B) Representative images of lentivirally transduced GFP-SNPH (1–469) (A) and GFP-SNPH (B) in PCs of SNPH-KO mice injected with saline (no harmaline, vehicle only). (C-H) Effect of harmaline on GFP-SNPH (1–469)-transduced (C) and GFP-SNPH-transduced (F) PC dendrites. Degenerating dendrites in GFP-SNPH-transduced PCs can be seen in (F). Also shown is Calbindin labeling of GFP SNPH (1–469) (D) and GFP-SNPH (G) from (C) and (F). Merged images of GFP SNPH (1–469) and GFP-SNPH with Calbindin are shown in (E) and (H), respectively. (I–K) Representative image of a harmaline-induced degenerating PC (white arrow in I) transduced with GFP-SNPH. Calbindin staining from the same section is shown in (J), whereas a merged image is shown in (K). Scale bars, 20 μm. (L) Quantification of dendritic shrinkage in GFP-SNPH (1–469)- and GFP-SNPH-transduced PCs in the absence (n = 3 mice, vehicle only) or presence of harmaline (n = 5 mice). Data are shown as mean ± SEM. ***p
    Figure Legend Snippet: SNPH Intrusion Sensitizes PC Dendrites to Glutamatergic Excitotoxicity In Vivo (A and B) Representative images of lentivirally transduced GFP-SNPH (1–469) (A) and GFP-SNPH (B) in PCs of SNPH-KO mice injected with saline (no harmaline, vehicle only). (C-H) Effect of harmaline on GFP-SNPH (1–469)-transduced (C) and GFP-SNPH-transduced (F) PC dendrites. Degenerating dendrites in GFP-SNPH-transduced PCs can be seen in (F). Also shown is Calbindin labeling of GFP SNPH (1–469) (D) and GFP-SNPH (G) from (C) and (F). Merged images of GFP SNPH (1–469) and GFP-SNPH with Calbindin are shown in (E) and (H), respectively. (I–K) Representative image of a harmaline-induced degenerating PC (white arrow in I) transduced with GFP-SNPH. Calbindin staining from the same section is shown in (J), whereas a merged image is shown in (K). Scale bars, 20 μm. (L) Quantification of dendritic shrinkage in GFP-SNPH (1–469)- and GFP-SNPH-transduced PCs in the absence (n = 3 mice, vehicle only) or presence of harmaline (n = 5 mice). Data are shown as mean ± SEM. ***p

    Techniques Used: In Vivo, Mouse Assay, Injection, Labeling, Transduction, Staining

    Related Articles

    Incubation:

    Article Title: Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration
    Article Snippet: Tissue sections from mice cerebellum were deparaffinized with xylene and hydrated through a series of alcohol washes and water, boiled for 20 min in citric acid based antigen unmasking solution (Vector laboratories), washed with PBS 3 times (5 min each) and incubated with blocking solution containing 0.3% Triton X-100, 10% normal goat serum prepared in PBS for 1 hr at room temperature (RT). .. Sections were then incubated with primary antibodies against SNPH (1:250; Abcam), Synaptotagmin2 (1:250; Developmental Studies Hybridoma Bank) and Calbindin (1:250; BosterBio) overnight in PBS containing 0.3% Triton X-100 and 5% normal goat serum. .. Following day, sections were washed 5 times with PBS (5 min each) and incubated with Alexa Fluor-labeled secondary antibodies (1:250; Invitrogen) for 1 hr at RT.

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    • calbindin  (Boster Bio)
    92
    Boster Bio calbindin
    Dendritic SNPH Intrusion in PCs of Dysmyelinating Shi Mice In Vivo (A–F) Immunohistochemistry technique for capturing SNPH intrusion in PC dendrites. Shown is SNPH (green), Syt2 (red), and <t>Calbindin</t> (blue) labeling in 3.5-month-old WT (A, C, and E) and Shi (B, D, and F) mice. Scale bar, 10 μm. (G and H) High magnification of the maximum intensity projection image from the z stack through dendritic regions of WT (G) and Shi (H). (I and J) Orthogonal (slice) view of SNPH punctum (indicated by arrows in panels G and H) in the dendritic region from WT (I) and Shi (J) in x-z and y-z orientations, respectively. (K) Quantification of percentage area occupied by SNPH within the dendritic volume from 3 mice of each group. Data are shown as mean ± SEM. *p
    Calbindin, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calbindin/product/Boster Bio
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calbindin - by Bioz Stars, 2021-06
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Dendritic SNPH Intrusion in PCs of Dysmyelinating Shi Mice In Vivo (A–F) Immunohistochemistry technique for capturing SNPH intrusion in PC dendrites. Shown is SNPH (green), Syt2 (red), and Calbindin (blue) labeling in 3.5-month-old WT (A, C, and E) and Shi (B, D, and F) mice. Scale bar, 10 μm. (G and H) High magnification of the maximum intensity projection image from the z stack through dendritic regions of WT (G) and Shi (H). (I and J) Orthogonal (slice) view of SNPH punctum (indicated by arrows in panels G and H) in the dendritic region from WT (I) and Shi (J) in x-z and y-z orientations, respectively. (K) Quantification of percentage area occupied by SNPH within the dendritic volume from 3 mice of each group. Data are shown as mean ± SEM. *p

    Journal: Cell reports

    Article Title: Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration

    doi: 10.1016/j.celrep.2019.09.012

    Figure Lengend Snippet: Dendritic SNPH Intrusion in PCs of Dysmyelinating Shi Mice In Vivo (A–F) Immunohistochemistry technique for capturing SNPH intrusion in PC dendrites. Shown is SNPH (green), Syt2 (red), and Calbindin (blue) labeling in 3.5-month-old WT (A, C, and E) and Shi (B, D, and F) mice. Scale bar, 10 μm. (G and H) High magnification of the maximum intensity projection image from the z stack through dendritic regions of WT (G) and Shi (H). (I and J) Orthogonal (slice) view of SNPH punctum (indicated by arrows in panels G and H) in the dendritic region from WT (I) and Shi (J) in x-z and y-z orientations, respectively. (K) Quantification of percentage area occupied by SNPH within the dendritic volume from 3 mice of each group. Data are shown as mean ± SEM. *p

    Article Snippet: Sections were then incubated with primary antibodies against SNPH (1:250; Abcam), Synaptotagmin2 (1:250; Developmental Studies Hybridoma Bank) and Calbindin (1:250; BosterBio) overnight in PBS containing 0.3% Triton X-100 and 5% normal goat serum.

    Techniques: Mouse Assay, In Vivo, Immunohistochemistry, Labeling

    SNPH Intrusion Sensitizes PC Dendrites to Glutamatergic Excitotoxicity In Vivo (A and B) Representative images of lentivirally transduced GFP-SNPH (1–469) (A) and GFP-SNPH (B) in PCs of SNPH-KO mice injected with saline (no harmaline, vehicle only). (C-H) Effect of harmaline on GFP-SNPH (1–469)-transduced (C) and GFP-SNPH-transduced (F) PC dendrites. Degenerating dendrites in GFP-SNPH-transduced PCs can be seen in (F). Also shown is Calbindin labeling of GFP SNPH (1–469) (D) and GFP-SNPH (G) from (C) and (F). Merged images of GFP SNPH (1–469) and GFP-SNPH with Calbindin are shown in (E) and (H), respectively. (I–K) Representative image of a harmaline-induced degenerating PC (white arrow in I) transduced with GFP-SNPH. Calbindin staining from the same section is shown in (J), whereas a merged image is shown in (K). Scale bars, 20 μm. (L) Quantification of dendritic shrinkage in GFP-SNPH (1–469)- and GFP-SNPH-transduced PCs in the absence (n = 3 mice, vehicle only) or presence of harmaline (n = 5 mice). Data are shown as mean ± SEM. ***p

    Journal: Cell reports

    Article Title: Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration

    doi: 10.1016/j.celrep.2019.09.012

    Figure Lengend Snippet: SNPH Intrusion Sensitizes PC Dendrites to Glutamatergic Excitotoxicity In Vivo (A and B) Representative images of lentivirally transduced GFP-SNPH (1–469) (A) and GFP-SNPH (B) in PCs of SNPH-KO mice injected with saline (no harmaline, vehicle only). (C-H) Effect of harmaline on GFP-SNPH (1–469)-transduced (C) and GFP-SNPH-transduced (F) PC dendrites. Degenerating dendrites in GFP-SNPH-transduced PCs can be seen in (F). Also shown is Calbindin labeling of GFP SNPH (1–469) (D) and GFP-SNPH (G) from (C) and (F). Merged images of GFP SNPH (1–469) and GFP-SNPH with Calbindin are shown in (E) and (H), respectively. (I–K) Representative image of a harmaline-induced degenerating PC (white arrow in I) transduced with GFP-SNPH. Calbindin staining from the same section is shown in (J), whereas a merged image is shown in (K). Scale bars, 20 μm. (L) Quantification of dendritic shrinkage in GFP-SNPH (1–469)- and GFP-SNPH-transduced PCs in the absence (n = 3 mice, vehicle only) or presence of harmaline (n = 5 mice). Data are shown as mean ± SEM. ***p

    Article Snippet: Sections were then incubated with primary antibodies against SNPH (1:250; Abcam), Synaptotagmin2 (1:250; Developmental Studies Hybridoma Bank) and Calbindin (1:250; BosterBio) overnight in PBS containing 0.3% Triton X-100 and 5% normal goat serum.

    Techniques: In Vivo, Mouse Assay, Injection, Labeling, Transduction, Staining