outer primers f3  (New England Biolabs)


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    Structured Review

    New England Biolabs outer primers f3
    Outer Primers F3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/outer primers f3/product/New England Biolabs
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    outer primers f3 - by Bioz Stars, 2020-04
    92/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: Diagnostic performance of a novel loop-mediated isothermal amplification (LAMP) assay targeting the apicoplast genome for malaria diagnosis in a field setting in sub-Saharan Africa
    Article Snippet: LAMP assay The final amplification reaction mixture (25 µL) was carried out as previously described [ ] and contained 1.6 µM each of the inner primers FIP and BIP, 0.2 µM each of the outer primers F3 and B3c, 0.8 µM each of the loop primers LPF and LPB, 1× Isothermal Amplification Buffer, 1 Unit of Bst 2.0 WarmStart™ DNA polymerase (New England Biolabs), 1.0 mM of each dNTPs, 1.0 mM MgCl2 , and 3 µL of DNA sample. .. The reaction was performed at 65 °C for 60 min in a water bath and the end product was determined and verified visually, after addition of SYBR Green I, (naked eye) for color change by two blinded operators (Fig. ).

    Article Title: Visual Detection of Enterohemorrhagic Escherichia coli O157:H7 Using Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP amplification and detection of products LAMP was performed in a 25-μl total reaction mixture containing the inner primers FIP and BIP at 0.8 mM, the outer primers F3 and B3 at 0.2 mM, loop primers LF and LB at 0.8 mM, 2.5 μl of 10X ThermoPol Reaction Buffer, each deoxynucleotide at 5.6 mM, 0.42 mM MgSO4, 0.5 M Beanie, 4 U of large fragment Bst DNA polymerase (New England Biolab), and 1 μl of template DNA. .. In addition, a positive amplification was indicated by a color change after adding 1 μl of SYBR Green II to the reaction mixture and observing under UV light.

    Negative Control:

    Article Title: Intermediate Hosts of Angiostrongylus cantonensis in Tenerife, Spain
    Article Snippet: Briefly, the assay was carried out in a 25 μl reaction system containing 10x Bst-DNA polymerase buffer (3 μl), deoxynucleotide triphosphates (0.5 mM for each), MgSO4 (2 mM), a forward inner primer (FIP) (5′- CTCATCATCAACCACCCACCCCTAGCATCATCTACGTCGTC-3′) and a backward inner primer (BIP) (5′-AGAAACCACCAACACATATACACGTATACCACCAACTTTAGCGA-3′) (1.6 μM for each), loop-F (5′- GGGTGGTGATGTAGTAGCTA-3′) and loop-B (5′- TCACCTAGTGTATGATGGT-3′) (0.8 μM for each), 0.4 μM of outer primers F3(5′- CCACCACAAAACACAAACA-3′) and B3 (5′-GTGTTGAGCTCTAACGGT-3′), Bst DNA polymerase (8 U) (New England BioLabs) and DNA template (1 μl, approximately 30 ng). .. A reaction system with no DNA template was used as the negative control.

    Amplification:

    Article Title: Development and Evaluation of Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Rapid and Real-Time Detection of the Swine-Origin Influenza A H1N1 Virus
    Article Snippet: .. The amplification of the HA gene of H1N1 virus was carried out in a total 25 μL reaction volume using 12.5 μL of in-house buffer [20 mmol/L Tris-HCl pH8.8; 10 mmol/L KCl; 10 mmol/L (NH4 )2 SO4 ; 8 mmol/L MgSO4 ; 1.4 mmol/L dNTPs; 0.8M betaine; 0.1% Tween- 20 ]; 3.75 μL c DNA mix (5x buffer, 10 mmol/L dNTP mix, and 0.25 μL of RNasin inhibitor); 1 μL of primer mix containing (50 pmol each of the primers FIP and BIP; 5 pmol each of the outer primers F3 and B3; 25 pmol each of loop primers FLP and BLP); 1 μL (8 units) of the BstDNA polymerase (New England Biolabs, Ipswich, MA); 0.25 μL of MMLV-RT; 1.5 μL of nuclease-free water; and 5 μL of RNA at 37°C for 30 minutes. .. The gene amplification was accomplished by incubating the reaction mixture at 63°C for 60 minutes in a routine laboratory water bath/dry heating bath.

    Article Title: Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls. Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls
    Article Snippet: Paragraph title: Loop‐mediated isothermal amplification ... LAMP was performed in a total reaction mixture volume of 25 μl of the following: 1.6 μM of each forward inner primer and backward inner primer, 0.2 μM of the outer primers F3 and B3, 0.4 μM of the loop primers LF and LB, 1.4 mM each of the four deoxynucleoside triphosphates, 8 U of the Bst DNA polymerase large fragment (New England Biolabs), 0.8 M betaine (Sigma), 20 mM Tris‐HCl (pH 8.8), 10 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween 20, and template DNA in a volume of up to 5.5 μl.

    Article Title: Environmental and Behavioral Risk Factors for Severe Leptospirosis in Thailand
    Article Snippet: Paragraph title: 2.4. Leptospira Loop–Mediated Isothermal Amplification Method (LAMP) ... This method used 10 ng of extracted DNA and a 25 µL reaction mixture containing 1.0 µM outer primers F3 and B3, 1.6 µM inner primers FIP and BIP, 0.8 µM loop primer LB, 1X Thermopol® reaction buffer (New England Biolabs, MA, USA), 4 mM MgSo4 (New England Biolabs, MA, USA), 1 M Betaine (Sigma, MO, USA), 0.4mMdNTPs (New England Biolabs, MA, USA), 0.5 mM MnCl, 25 µM Calcein, and 8 units Bst DNA polymerase (New England Biolabs, MA, USA).

    Article Title: Diagnostic performance of a novel loop-mediated isothermal amplification (LAMP) assay targeting the apicoplast genome for malaria diagnosis in a field setting in sub-Saharan Africa
    Article Snippet: .. LAMP assay The final amplification reaction mixture (25 µL) was carried out as previously described [ ] and contained 1.6 µM each of the inner primers FIP and BIP, 0.2 µM each of the outer primers F3 and B3c, 0.8 µM each of the loop primers LPF and LPB, 1× Isothermal Amplification Buffer, 1 Unit of Bst 2.0 WarmStart™ DNA polymerase (New England Biolabs), 1.0 mM of each dNTPs, 1.0 mM MgCl2 , and 3 µL of DNA sample. .. The reaction was performed at 65 °C for 60 min in a water bath and the end product was determined and verified visually, after addition of SYBR Green I, (naked eye) for color change by two blinded operators (Fig. ).

    Article Title: Intermediate Hosts of Angiostrongylus cantonensis in Tenerife, Spain
    Article Snippet: Briefly, the assay was carried out in a 25 μl reaction system containing 10x Bst-DNA polymerase buffer (3 μl), deoxynucleotide triphosphates (0.5 mM for each), MgSO4 (2 mM), a forward inner primer (FIP) (5′- CTCATCATCAACCACCCACCCCTAGCATCATCTACGTCGTC-3′) and a backward inner primer (BIP) (5′-AGAAACCACCAACACATATACACGTATACCACCAACTTTAGCGA-3′) (1.6 μM for each), loop-F (5′- GGGTGGTGATGTAGTAGCTA-3′) and loop-B (5′- TCACCTAGTGTATGATGGT-3′) (0.8 μM for each), 0.4 μM of outer primers F3(5′- CCACCACAAAACACAAACA-3′) and B3 (5′-GTGTTGAGCTCTAACGGT-3′), Bst DNA polymerase (8 U) (New England BioLabs) and DNA template (1 μl, approximately 30 ng). .. LAMP amplification results were visually detected under UV light after adding 2 μl of 20x EvaGreen I (Biotium) to the reaction tubes.

    Article Title: Visual Detection of Enterohemorrhagic Escherichia coli O157:H7 Using Loop-Mediated Isothermal Amplification
    Article Snippet: .. LAMP amplification and detection of products LAMP was performed in a 25-μl total reaction mixture containing the inner primers FIP and BIP at 0.8 mM, the outer primers F3 and B3 at 0.2 mM, loop primers LF and LB at 0.8 mM, 2.5 μl of 10X ThermoPol Reaction Buffer, each deoxynucleotide at 5.6 mM, 0.42 mM MgSO4, 0.5 M Beanie, 4 U of large fragment Bst DNA polymerase (New England Biolab), and 1 μl of template DNA. .. LAMP products were subjected to electrophoresis on a 2% agarose gel, stained by ethidium bromide, and then examined under UV light.

    Agarose Gel Electrophoresis:

    Article Title: Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls. Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls
    Article Snippet: LAMP was performed in a total reaction mixture volume of 25 μl of the following: 1.6 μM of each forward inner primer and backward inner primer, 0.2 μM of the outer primers F3 and B3, 0.4 μM of the loop primers LF and LB, 1.4 mM each of the four deoxynucleoside triphosphates, 8 U of the Bst DNA polymerase large fragment (New England Biolabs), 0.8 M betaine (Sigma), 20 mM Tris‐HCl (pH 8.8), 10 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween 20, and template DNA in a volume of up to 5.5 μl. .. LAMP amplicon was detected by visual inspection using a turbidity derived from the white precipitate of magnesium pyrophosphate and by 3% agarose gel electrophoresis and followed by staining with ethidium bromide.

    Article Title: Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls. Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls
    Article Snippet: LAMP was performed in a total reaction mixture volume of 25 μl of the following: 1.6 μM of each forward inner primer and backward inner primer, 0.2 μM of the outer primers F3 and B3, 0.4 μM of the loop primers LF and LB, 1.4 mM each of the four deoxynucleoside triphosphates, 8 U of the Bst DNA polymerase large fragment (New England Biolabs), 0.8 M betaine (Sigma), 20 mM Tris‐HCl (pH 8.8), 10 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween 20, and template DNA in a volume of up to 5.5 μl. .. LAMP amplicon was detected by visual inspection using a turbidity derived from the white precipitate of magnesium pyrophosphate and by 3% agarose gel electrophoresis and followed by staining with ethidium bromide.

    Article Title: Loop-mediated isothermal amplification for detection of porcine circovirus type 2
    Article Snippet: The optimized LAMP assay The optimum LAMP reaction mixture (25 μL) contained 2.4 μM (each) of inner primers FIP and BIP, 0.24 μM (each) of outer primers F3 and B3, 0.6 mM each deoxynucleoside triphosphate, 0.4 M betaine, 1 × ThermoPol buffer (20 mM Tris-HCl, 10 mM KCl, 10 mM (NH4 )2 SO4 , 2 mM MgSO4 , 0.1% Triton X-100), 8 U of Bst DNA polymerase (New England Biolabs, USA) and 1 μL of template DNA. .. The result of agarose gel electrophoresis showed that a characteristic ladder-like pattern of bands was clearest when the reaction was performed at 63°C (Figure ) [ ].

    Article Title: Visual Detection of Enterohemorrhagic Escherichia coli O157:H7 Using Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP amplification and detection of products LAMP was performed in a 25-μl total reaction mixture containing the inner primers FIP and BIP at 0.8 mM, the outer primers F3 and B3 at 0.2 mM, loop primers LF and LB at 0.8 mM, 2.5 μl of 10X ThermoPol Reaction Buffer, each deoxynucleotide at 5.6 mM, 0.42 mM MgSO4, 0.5 M Beanie, 4 U of large fragment Bst DNA polymerase (New England Biolab), and 1 μl of template DNA. .. LAMP products were subjected to electrophoresis on a 2% agarose gel, stained by ethidium bromide, and then examined under UV light.

    RT Lamp Assay:

    Article Title: Rapid colorimetric detection of Zika virus from serum and urine specimens by reverse transcription loop-mediated isothermal amplification (RT-LAMP)
    Article Snippet: .. RT-LAMP assay RT-LAMP reactions were carried out in triplicate in a 25 μl volume containing 1.6 μM each of inner primers FIP and BIP, 0.2 μM each of outer primers F3 and B3, 0.4 μM each of loop primers FL and BL, and either 12.5 μl of 2X Colorimetric LAMP Master Mix (Cat. No. M1800, New England Biolabs) and 10 μl of RNA template, or 5.0 μl of a custom-made 5X Colorimetric LAMP Master Mix and 17.5 μl of RNA template [ , ]. ..

    Blocking Assay:

    Article Title: Environmental and Behavioral Risk Factors for Severe Leptospirosis in Thailand
    Article Snippet: This method used 10 ng of extracted DNA and a 25 µL reaction mixture containing 1.0 µM outer primers F3 and B3, 1.6 µM inner primers FIP and BIP, 0.8 µM loop primer LB, 1X Thermopol® reaction buffer (New England Biolabs, MA, USA), 4 mM MgSo4 (New England Biolabs, MA, USA), 1 M Betaine (Sigma, MO, USA), 0.4mMdNTPs (New England Biolabs, MA, USA), 0.5 mM MnCl, 25 µM Calcein, and 8 units Bst DNA polymerase (New England Biolabs, MA, USA). .. The reaction mixture was incubated at 61 °C for 90–120 min in a heating block and then heated at 80 °C for 2 min to terminate the reaction.

    Electrophoresis:

    Article Title: Visual Detection of Enterohemorrhagic Escherichia coli O157:H7 Using Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP amplification and detection of products LAMP was performed in a 25-μl total reaction mixture containing the inner primers FIP and BIP at 0.8 mM, the outer primers F3 and B3 at 0.2 mM, loop primers LF and LB at 0.8 mM, 2.5 μl of 10X ThermoPol Reaction Buffer, each deoxynucleotide at 5.6 mM, 0.42 mM MgSO4, 0.5 M Beanie, 4 U of large fragment Bst DNA polymerase (New England Biolab), and 1 μl of template DNA. .. LAMP products were subjected to electrophoresis on a 2% agarose gel, stained by ethidium bromide, and then examined under UV light.

    Incubation:

    Article Title: Development of reverse-transcription loop-mediated isothermal amplification assay for rapid detection and differentiation of dengue virus serotypes 1–4
    Article Snippet: .. RT-LAMP assays The RT-LAMP reaction was carried out in a total 25 μL reaction mixture containing 40 pM each of primers FIP and BIP, 5 pmol each of outer primers F3 and B3, 20 pM each of FLP and BLP, 1.4 mM deoxynucleoside triphosphates, 0.8 M betaine, 0.1 % Tween 20, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 10 mM KCl, 20 mM Tris–HCl, pH 8.8, 16 U of Bst DNA polymerase (New England Biolabs), 0.125 U of avian myeloblastosis virus reverse transcriptase (Invitrogen), and different copies of genomic RNA templates in sensitivity assays or about 100 ng of target RNA in in the others experiments, which were incubated at 63 °C for 45 min. .. Detection of RT-LAMP products For naked-eye detection, 1.0 μL of 10−1 diluted SYBR Green I (Takara Bio Inc., Otsu, Japan) was dropped on the inner cover of the reaction tube to avoid the aerosol pollution, and then mixed the SYBR Green I and the reaction mixture to observe the color.

    Article Title: Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls. Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls
    Article Snippet: LAMP was performed in a total reaction mixture volume of 25 μl of the following: 1.6 μM of each forward inner primer and backward inner primer, 0.2 μM of the outer primers F3 and B3, 0.4 μM of the loop primers LF and LB, 1.4 mM each of the four deoxynucleoside triphosphates, 8 U of the Bst DNA polymerase large fragment (New England Biolabs), 0.8 M betaine (Sigma), 20 mM Tris‐HCl (pH 8.8), 10 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween 20, and template DNA in a volume of up to 5.5 μl. .. The mixtures were incubated at 63, 64, and 65 °C using a thermocycler for 60 min and then heated at 80 °C for 2 min to terminate the reaction.

    Article Title: Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls. Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls
    Article Snippet: LAMP was performed in a total reaction mixture volume of 25 μl of the following: 1.6 μM of each forward inner primer and backward inner primer, 0.2 μM of the outer primers F3 and B3, 0.4 μM of the loop primers LF and LB, 1.4 mM each of the four deoxynucleoside triphosphates, 8 U of the Bst DNA polymerase large fragment (New England Biolabs), 0.8 M betaine (Sigma), 20 mM Tris‐HCl (pH 8.8), 10 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween 20, and template DNA in a volume of up to 5.5 μl. .. The mixtures were incubated at 63, 64, and 65 °C using a thermocycler for 60 min and then heated at 80 °C for 2 min to terminate the reaction.

    Article Title: Environmental and Behavioral Risk Factors for Severe Leptospirosis in Thailand
    Article Snippet: This method used 10 ng of extracted DNA and a 25 µL reaction mixture containing 1.0 µM outer primers F3 and B3, 1.6 µM inner primers FIP and BIP, 0.8 µM loop primer LB, 1X Thermopol® reaction buffer (New England Biolabs, MA, USA), 4 mM MgSo4 (New England Biolabs, MA, USA), 1 M Betaine (Sigma, MO, USA), 0.4mMdNTPs (New England Biolabs, MA, USA), 0.5 mM MnCl, 25 µM Calcein, and 8 units Bst DNA polymerase (New England Biolabs, MA, USA). .. The reaction mixture was incubated at 61 °C for 90–120 min in a heating block and then heated at 80 °C for 2 min to terminate the reaction.

    Lamp Assay:

    Article Title: Environmental and Behavioral Risk Factors for Severe Leptospirosis in Thailand
    Article Snippet: The LAMP assay was carried out using primers and conditions previously described by Suwancharoen et al. [ ]. .. This method used 10 ng of extracted DNA and a 25 µL reaction mixture containing 1.0 µM outer primers F3 and B3, 1.6 µM inner primers FIP and BIP, 0.8 µM loop primer LB, 1X Thermopol® reaction buffer (New England Biolabs, MA, USA), 4 mM MgSo4 (New England Biolabs, MA, USA), 1 M Betaine (Sigma, MO, USA), 0.4mMdNTPs (New England Biolabs, MA, USA), 0.5 mM MnCl, 25 µM Calcein, and 8 units Bst DNA polymerase (New England Biolabs, MA, USA).

    Article Title: Development of Loop-Mediated Isothermal Amplification (LAMP) for Universal Detection of Enteroviruses
    Article Snippet: Paragraph title: LAMP Assay ... The reaction was carried out in a 25 μl mixture containing 1.6 μM each of the inner primers FIP and BIP, 0.2 μM each of the outer primers F3 and B3, 2.5 μl of 10 × Bst DNA polymerase reaction buffer, 8 U Bst DNA polymerase (NEB, U.S.), 1.8 mM dNTPs, 0.8 M betaine, 2 mM MgCl2 and 5 μl of target sample.

    Article Title: Loop-mediated isothermal amplification for detection of porcine circovirus type 2
    Article Snippet: .. The optimized LAMP assay The optimum LAMP reaction mixture (25 μL) contained 2.4 μM (each) of inner primers FIP and BIP, 0.24 μM (each) of outer primers F3 and B3, 0.6 mM each deoxynucleoside triphosphate, 0.4 M betaine, 1 × ThermoPol buffer (20 mM Tris-HCl, 10 mM KCl, 10 mM (NH4 )2 SO4 , 2 mM MgSO4 , 0.1% Triton X-100), 8 U of Bst DNA polymerase (New England Biolabs, USA) and 1 μL of template DNA. .. The result of agarose gel electrophoresis showed that a characteristic ladder-like pattern of bands was clearest when the reaction was performed at 63°C (Figure ) [ ].

    Article Title: Diagnostic performance of a novel loop-mediated isothermal amplification (LAMP) assay targeting the apicoplast genome for malaria diagnosis in a field setting in sub-Saharan Africa
    Article Snippet: .. LAMP assay The final amplification reaction mixture (25 µL) was carried out as previously described [ ] and contained 1.6 µM each of the inner primers FIP and BIP, 0.2 µM each of the outer primers F3 and B3c, 0.8 µM each of the loop primers LPF and LPB, 1× Isothermal Amplification Buffer, 1 Unit of Bst 2.0 WarmStart™ DNA polymerase (New England Biolabs), 1.0 mM of each dNTPs, 1.0 mM MgCl2 , and 3 µL of DNA sample. .. The reaction was performed at 65 °C for 60 min in a water bath and the end product was determined and verified visually, after addition of SYBR Green I, (naked eye) for color change by two blinded operators (Fig. ).

    Article Title: Intermediate Hosts of Angiostrongylus cantonensis in Tenerife, Spain
    Article Snippet: LAMP method for the detection of A . cantonensis in mollusc tissue The LAMP assay was performed according to [ ]. .. Briefly, the assay was carried out in a 25 μl reaction system containing 10x Bst-DNA polymerase buffer (3 μl), deoxynucleotide triphosphates (0.5 mM for each), MgSO4 (2 mM), a forward inner primer (FIP) (5′- CTCATCATCAACCACCCACCCCTAGCATCATCTACGTCGTC-3′) and a backward inner primer (BIP) (5′-AGAAACCACCAACACATATACACGTATACCACCAACTTTAGCGA-3′) (1.6 μM for each), loop-F (5′- GGGTGGTGATGTAGTAGCTA-3′) and loop-B (5′- TCACCTAGTGTATGATGGT-3′) (0.8 μM for each), 0.4 μM of outer primers F3(5′- CCACCACAAAACACAAACA-3′) and B3 (5′-GTGTTGAGCTCTAACGGT-3′), Bst DNA polymerase (8 U) (New England BioLabs) and DNA template (1 μl, approximately 30 ng).

    Modification:

    Article Title: Rapid, simple and direct detection of Meloidogyne hapla from infected root galls using loop-mediated isothermal amplification combined with FTA technology
    Article Snippet: Optimization of the LAMP reaction The LAMP reaction was performed according to the protocol published previously with a minor modification. .. In a brief, the procedure used a 25 μL LAMP reaction mixture containing 1.4 μM each of the inner primers FIP and BIP, 0.2 μM each of the outer primers F3 and B3, 0.8 μM of the LF primer (forward loop primer), (0, 0.4, 0.8, 1.2, 1.6, 2.0, 2.4 mM) of a dNTPs mix, (2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0 mM) MgSO4 , (0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6 M) betaine, 8U of Bst DNA polymerase (New England Biolabs GmbH, USA), 2.5 μL of 10×Thermopol reaction buffer (20 mM Tris-HCl (pH 8.8, 25 °C), 10 mM KCl, 10 mM (NH4 )2 SO4 , 2 mM MgSO4 , 0.1% Triton X-100), 1 μL of genomic DNA solution, and double distilled water was added to reach a total volume of 25 μL.

    Staining:

    Article Title: Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls. Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls
    Article Snippet: LAMP was performed in a total reaction mixture volume of 25 μl of the following: 1.6 μM of each forward inner primer and backward inner primer, 0.2 μM of the outer primers F3 and B3, 0.4 μM of the loop primers LF and LB, 1.4 mM each of the four deoxynucleoside triphosphates, 8 U of the Bst DNA polymerase large fragment (New England Biolabs), 0.8 M betaine (Sigma), 20 mM Tris‐HCl (pH 8.8), 10 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween 20, and template DNA in a volume of up to 5.5 μl. .. LAMP amplicon was detected by visual inspection using a turbidity derived from the white precipitate of magnesium pyrophosphate and by 3% agarose gel electrophoresis and followed by staining with ethidium bromide.

    Article Title: Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls. Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls
    Article Snippet: LAMP was performed in a total reaction mixture volume of 25 μl of the following: 1.6 μM of each forward inner primer and backward inner primer, 0.2 μM of the outer primers F3 and B3, 0.4 μM of the loop primers LF and LB, 1.4 mM each of the four deoxynucleoside triphosphates, 8 U of the Bst DNA polymerase large fragment (New England Biolabs), 0.8 M betaine (Sigma), 20 mM Tris‐HCl (pH 8.8), 10 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween 20, and template DNA in a volume of up to 5.5 μl. .. LAMP amplicon was detected by visual inspection using a turbidity derived from the white precipitate of magnesium pyrophosphate and by 3% agarose gel electrophoresis and followed by staining with ethidium bromide.

    Article Title: Visual Detection of Enterohemorrhagic Escherichia coli O157:H7 Using Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP amplification and detection of products LAMP was performed in a 25-μl total reaction mixture containing the inner primers FIP and BIP at 0.8 mM, the outer primers F3 and B3 at 0.2 mM, loop primers LF and LB at 0.8 mM, 2.5 μl of 10X ThermoPol Reaction Buffer, each deoxynucleotide at 5.6 mM, 0.42 mM MgSO4, 0.5 M Beanie, 4 U of large fragment Bst DNA polymerase (New England Biolab), and 1 μl of template DNA. .. LAMP products were subjected to electrophoresis on a 2% agarose gel, stained by ethidium bromide, and then examined under UV light.

    Derivative Assay:

    Article Title: Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls. Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls
    Article Snippet: LAMP was performed in a total reaction mixture volume of 25 μl of the following: 1.6 μM of each forward inner primer and backward inner primer, 0.2 μM of the outer primers F3 and B3, 0.4 μM of the loop primers LF and LB, 1.4 mM each of the four deoxynucleoside triphosphates, 8 U of the Bst DNA polymerase large fragment (New England Biolabs), 0.8 M betaine (Sigma), 20 mM Tris‐HCl (pH 8.8), 10 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween 20, and template DNA in a volume of up to 5.5 μl. .. LAMP amplicon was detected by visual inspection using a turbidity derived from the white precipitate of magnesium pyrophosphate and by 3% agarose gel electrophoresis and followed by staining with ethidium bromide.

    Article Title: Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls. Putative periodontopathic bacteria and herpes viruses interactions in the subgingival plaque of patients with aggressive periodontitis and healthy controls
    Article Snippet: LAMP was performed in a total reaction mixture volume of 25 μl of the following: 1.6 μM of each forward inner primer and backward inner primer, 0.2 μM of the outer primers F3 and B3, 0.4 μM of the loop primers LF and LB, 1.4 mM each of the four deoxynucleoside triphosphates, 8 U of the Bst DNA polymerase large fragment (New England Biolabs), 0.8 M betaine (Sigma), 20 mM Tris‐HCl (pH 8.8), 10 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween 20, and template DNA in a volume of up to 5.5 μl. .. LAMP amplicon was detected by visual inspection using a turbidity derived from the white precipitate of magnesium pyrophosphate and by 3% agarose gel electrophoresis and followed by staining with ethidium bromide.

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    New England Biolabs warmstart colorimetric lamp 2x master mix dna and rna
    Warmstart Colorimetric Lamp 2x Master Mix Dna And Rna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/warmstart colorimetric lamp 2x master mix dna and rna/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    warmstart colorimetric lamp 2x master mix dna and rna - by Bioz Stars, 2020-04
    99/100 stars
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