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New England Biolabs t4 bgt
T4 Bgt, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 bgt/product/New England Biolabs
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
t4 bgt - by Bioz Stars, 2019-06
93/100 stars

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Related Articles

Conjugation Assay:

Article Title: Selective modulation of local linkages between active transcription and oxidative demethylation activity shapes cardiomyocyte-specific gene-body epigenetic status in mice
Article Snippet: For the sequencing analysis of pull-down DNA (BGT-seq or hMe_Seal) [ ], genomic DNA was fragmented to a length of approximately 300 bp using the Covaris system. .. Fragmented DNA and UDP-azide-glucose were incubated overnight with T4 BGT (NEB) at 37 °C followed by biotin conjugation. .. Biotinylated DNA was then purified, captured with streptavidin beads, washed and eluted.

Article Title: Selective modulation of local linkages between active transcription and oxidative demethylation activity shapes cardiomyocyte-specific gene-body epigenetic status in mice
Article Snippet: In brief, aliquots of fragmented DNA were incubated overnight with or without 30 units of T4 BGT (NEB) at 37 °C, and some of the aliquots were then digested using 50 units of MspI. .. For the sequencing analysis of pull-down DNA (BGT-seq or hMe_Seal) [ ], genomic DNA was fragmented to a length of approximately 300 bp using the Covaris system.

Isolation:

Article Title: Simultaneous sequencing of oxidized methylcytosines produced by TET/JBP dioxygenases in Coprinopsis cinerea
Article Snippet: Genomic DNA was isolated from C. cinerea oidia protoplast cells by using DNeasy plant maxi-kit (Qiagen) following the manufacturer’s instructions. .. A maximum of 1 µg fragmented DNA was treated with 8 U T4-BGT (M0357; NEB) and 200 ng recombinant BGAGT in 100 µL of 100 mM Tris, pH 7.4, 50 µM UDP-glucose (NEB), 40 mM β-mercaptoethanol, and 25 mM MgCl2 for 2 h at 30 °C.

Incubation:

Article Title: Selective modulation of local linkages between active transcription and oxidative demethylation activity shapes cardiomyocyte-specific gene-body epigenetic status in mice
Article Snippet: For the sequencing analysis of pull-down DNA (BGT-seq or hMe_Seal) [ ], genomic DNA was fragmented to a length of approximately 300 bp using the Covaris system. .. Fragmented DNA and UDP-azide-glucose were incubated overnight with T4 BGT (NEB) at 37 °C followed by biotin conjugation. .. Biotinylated DNA was then purified, captured with streptavidin beads, washed and eluted.

Article Title: Selective modulation of local linkages between active transcription and oxidative demethylation activity shapes cardiomyocyte-specific gene-body epigenetic status in mice
Article Snippet: For the BGT assay, a 5mC and 5hmC detection kit (NEB) was employed according to the manufacturer’s instructions with minor modifications. .. In brief, aliquots of fragmented DNA were incubated overnight with or without 30 units of T4 BGT (NEB) at 37 °C, and some of the aliquots were then digested using 50 units of MspI. .. The three aliquots were treated with A) T4 BGT + MspI, B) with T4 BGT alone, or C) with MspI alone, and the percentage of 5hmC was calculated as %(A-C)/(B-C).

Construct:

Article Title: Selective modulation of local linkages between active transcription and oxidative demethylation activity shapes cardiomyocyte-specific gene-body epigenetic status in mice
Article Snippet: Fragmented DNA and UDP-azide-glucose were incubated overnight with T4 BGT (NEB) at 37 °C followed by biotin conjugation. .. Biotinylated DNA was then purified, captured with streptavidin beads, washed and eluted.

Purification:

Article Title: Selective modulation of local linkages between active transcription and oxidative demethylation activity shapes cardiomyocyte-specific gene-body epigenetic status in mice
Article Snippet: Genomic DNA was divided into fragments of 200 to 500 bp using a Covaris system (Covaris Inc.) and was purified using AMPure (Agencourt). .. Fragmented DNA and UDP-azide-glucose were incubated overnight with T4 BGT (NEB) at 37 °C followed by biotin conjugation.

Article Title: Simultaneous sequencing of oxidized methylcytosines produced by TET/JBP dioxygenases in Coprinopsis cinerea
Article Snippet: Purified genomic DNA was sheared to ∼400–500 bp fragments by using Covaris S2. .. A maximum of 1 µg fragmented DNA was treated with 8 U T4-BGT (M0357; NEB) and 200 ng recombinant BGAGT in 100 µL of 100 mM Tris, pH 7.4, 50 µM UDP-glucose (NEB), 40 mM β-mercaptoethanol, and 25 mM MgCl2 for 2 h at 30 °C.

Article Title: Simultaneous sequencing of oxidized methylcytosines produced by TET/JBP dioxygenases in Coprinopsis cinerea
Article Snippet: DNA was purified using the MinElute PCR purification kit (Qiagen) and then converted into a SMRTbell library for sequencing using the PacBio RS system. .. Control experiments with a purified 5hmC-containing oligonucleotide ( ) showed that both glucosylation steps catalyzed by T4-BGT and T6-BGAGT resulted in essentially complete modification at each step. .. For preparation of C. cinerea genomic DNA before dot blot estimation of 5hmC, 5fC, and 5caC, 107 oidia were lysed in 1.5 mL 40 mM Tris, pH 8, 40 mM EDTA, 0.5% SDS containing 100 mg RNAseA/mL and incubated for 30 min at 37 °C.

Real-time Polymerase Chain Reaction:

Article Title: Selective modulation of local linkages between active transcription and oxidative demethylation activity shapes cardiomyocyte-specific gene-body epigenetic status in mice
Article Snippet: Fragmented DNA and UDP-azide-glucose were incubated overnight with T4 BGT (NEB) at 37 °C followed by biotin conjugation. .. The eluted DNA was then purified and employed to construct a DNA library using the ChIP-seq Library Prep Master Mix Set with Q5 (NEB).

Sequencing:

Article Title: Selective modulation of local linkages between active transcription and oxidative demethylation activity shapes cardiomyocyte-specific gene-body epigenetic status in mice
Article Snippet: For the sequencing analysis of pull-down DNA (BGT-seq or hMe_Seal) [ ], genomic DNA was fragmented to a length of approximately 300 bp using the Covaris system. .. Fragmented DNA and UDP-azide-glucose were incubated overnight with T4 BGT (NEB) at 37 °C followed by biotin conjugation.

Article Title: Selective modulation of local linkages between active transcription and oxidative demethylation activity shapes cardiomyocyte-specific gene-body epigenetic status in mice
Article Snippet: In brief, aliquots of fragmented DNA were incubated overnight with or without 30 units of T4 BGT (NEB) at 37 °C, and some of the aliquots were then digested using 50 units of MspI. .. The three aliquots were treated with A) T4 BGT + MspI, B) with T4 BGT alone, or C) with MspI alone, and the percentage of 5hmC was calculated as %(A-C)/(B-C).

Generated:

Article Title: Selective modulation of local linkages between active transcription and oxidative demethylation activity shapes cardiomyocyte-specific gene-body epigenetic status in mice
Article Snippet: Fragmented DNA and UDP-azide-glucose were incubated overnight with T4 BGT (NEB) at 37 °C followed by biotin conjugation. .. Fragmented DNA and UDP-azide-glucose were incubated overnight with T4 BGT (NEB) at 37 °C followed by biotin conjugation.

Modification:

Article Title: Simultaneous sequencing of oxidized methylcytosines produced by TET/JBP dioxygenases in Coprinopsis cinerea
Article Snippet: A maximum of 1 µg fragmented DNA was treated with 8 U T4-BGT (M0357; NEB) and 200 ng recombinant BGAGT in 100 µL of 100 mM Tris, pH 7.4, 50 µM UDP-glucose (NEB), 40 mM β-mercaptoethanol, and 25 mM MgCl2 for 2 h at 30 °C. .. DNA was purified using the MinElute PCR purification kit (Qiagen) and then converted into a SMRTbell library for sequencing using the PacBio RS system.

Article Title: Simultaneous sequencing of oxidized methylcytosines produced by TET/JBP dioxygenases in Coprinopsis cinerea
Article Snippet: DNA was purified using the MinElute PCR purification kit (Qiagen) and then converted into a SMRTbell library for sequencing using the PacBio RS system. .. Control experiments with a purified 5hmC-containing oligonucleotide ( ) showed that both glucosylation steps catalyzed by T4-BGT and T6-BGAGT resulted in essentially complete modification at each step. .. For preparation of C. cinerea genomic DNA before dot blot estimation of 5hmC, 5fC, and 5caC, 107 oidia were lysed in 1.5 mL 40 mM Tris, pH 8, 40 mM EDTA, 0.5% SDS containing 100 mg RNAseA/mL and incubated for 30 min at 37 °C.

Recombinant:

Article Title: Simultaneous sequencing of oxidized methylcytosines produced by TET/JBP dioxygenases in Coprinopsis cinerea
Article Snippet: Purified genomic DNA was sheared to ∼400–500 bp fragments by using Covaris S2. .. A maximum of 1 µg fragmented DNA was treated with 8 U T4-BGT (M0357; NEB) and 200 ng recombinant BGAGT in 100 µL of 100 mM Tris, pH 7.4, 50 µM UDP-glucose (NEB), 40 mM β-mercaptoethanol, and 25 mM MgCl2 for 2 h at 30 °C. .. DNA was purified using the MinElute PCR purification kit (Qiagen) and then converted into a SMRTbell library for sequencing using the PacBio RS system.

ChIP-sequencing:

Article Title: Selective modulation of local linkages between active transcription and oxidative demethylation activity shapes cardiomyocyte-specific gene-body epigenetic status in mice
Article Snippet: Fragmented DNA and UDP-azide-glucose were incubated overnight with T4 BGT (NEB) at 37 °C followed by biotin conjugation. .. Biotinylated DNA was then purified, captured with streptavidin beads, washed and eluted.

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    New England Biolabs t4 bgt
    T4 Bgt, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 bgt/product/New England Biolabs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    t4 bgt - by Bioz Stars, 2019-06
    93/100 stars
      Buy from Supplier

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