poly a polymerase  (New England Biolabs)


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  • 99
    Name:
    Poly U Polymerase
    Description:
    Poly U Polymerase 60 units
    Catalog Number:
    m0337s
    Price:
    90
    Size:
    60 units
    Category:
    RNA Polymerases
    Buy from Supplier


    Structured Review

    New England Biolabs poly a polymerase
    Poly U Polymerase
    Poly U Polymerase 60 units
    https://www.bioz.com/result/poly a polymerase/product/New England Biolabs
    Average 99 stars, based on 114 article reviews
    Price from $9.99 to $1999.99
    poly a polymerase - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿ †
    Article Snippet: .. Full-length Ago1 was amplified by standard PCR techniques using vent polymerase (New England Biolabs) and cloned between the EcoRI and BamHI restriction sites of the pFlag-CMV2 (Sigma) vector. .. MS2 fusion constructs were generated by PCR amplification of the MS2-binding domain and cloning into the HindIII restriction site of the different pFlag-CMV2 constructs.

    Amplification:

    Article Title: Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿ †
    Article Snippet: .. Full-length Ago1 was amplified by standard PCR techniques using vent polymerase (New England Biolabs) and cloned between the EcoRI and BamHI restriction sites of the pFlag-CMV2 (Sigma) vector. .. MS2 fusion constructs were generated by PCR amplification of the MS2-binding domain and cloning into the HindIII restriction site of the different pFlag-CMV2 constructs.

    Article Title: Circularization pathway of a bacterial group II intron
    Article Snippet: .. For Ll.LtrB-ΔA a second amplification of the 3′ end was performed where a polyU instead of a polyA tail was added using 2U of Poly(U) Polymerase (New England Biolabs) (10 min at 37°C). ..

    Article Title: Serotype-specific interactions among functional domains of dengue virus 2 nonstructural proteins (NS) 5 and NS3 are crucial for viral RNA replication
    Article Snippet: .. The synthesized viral cDNA from collected supernatants was amplified by PCR using NEBNext High Fidelity 2× polymerase (New England Biolabs) and the viral sequence-specific primers (Ref. and ) to make overlapping fragments (each ∼800 bp) that spanned the entire genome. .. PCR products were purified by agarose gel (Zymo Research), and the nucleotide sequence of each fragment was determined by GENEWIZ Inc.

    In Vitro:

    Article Title: An Unusual Two-Step Control of CPEB Destruction by Pin1
    Article Snippet: .. Fifty oocytes [some injected with 10 ng of mRNA synthesized with an Ambion mMESSAGE kit and polyadenylated in vitro with Escherichia coli poly(A) polymerase (New England BioLabs)] or HEK293T cells from one 10-cm dish were homogenized in lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% deoxycholate, 0.1% SDS, 50 mM Tris-HCl (pH 7.7), 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM dithiothreitol, and protease inhibitor cocktail (Roche). .. The lysis buffer in some experiments also contained 10 μg/ml RNase A (Sigma).

    Synthesized:

    Article Title: Serotype-specific interactions among functional domains of dengue virus 2 nonstructural proteins (NS) 5 and NS3 are crucial for viral RNA replication
    Article Snippet: .. The synthesized viral cDNA from collected supernatants was amplified by PCR using NEBNext High Fidelity 2× polymerase (New England Biolabs) and the viral sequence-specific primers (Ref. and ) to make overlapping fragments (each ∼800 bp) that spanned the entire genome. .. PCR products were purified by agarose gel (Zymo Research), and the nucleotide sequence of each fragment was determined by GENEWIZ Inc.

    Article Title: An Unusual Two-Step Control of CPEB Destruction by Pin1
    Article Snippet: .. Fifty oocytes [some injected with 10 ng of mRNA synthesized with an Ambion mMESSAGE kit and polyadenylated in vitro with Escherichia coli poly(A) polymerase (New England BioLabs)] or HEK293T cells from one 10-cm dish were homogenized in lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% deoxycholate, 0.1% SDS, 50 mM Tris-HCl (pH 7.7), 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM dithiothreitol, and protease inhibitor cocktail (Roche). .. The lysis buffer in some experiments also contained 10 μg/ml RNase A (Sigma).

    Mutagenesis:

    Article Title: Sequences in the U3 region of Human Immunodeficiency Virus 1 improve efficiency of minus strand transfer in infected cells
    Article Snippet: .. For mutant M9-m, two PCR products were produced using pairs of oligomers 1/3 and 4/2 and VENT polymerase (NewEngland Biolabs). ..

    Protease Inhibitor:

    Article Title: An Unusual Two-Step Control of CPEB Destruction by Pin1
    Article Snippet: .. Fifty oocytes [some injected with 10 ng of mRNA synthesized with an Ambion mMESSAGE kit and polyadenylated in vitro with Escherichia coli poly(A) polymerase (New England BioLabs)] or HEK293T cells from one 10-cm dish were homogenized in lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% deoxycholate, 0.1% SDS, 50 mM Tris-HCl (pH 7.7), 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM dithiothreitol, and protease inhibitor cocktail (Roche). .. The lysis buffer in some experiments also contained 10 μg/ml RNase A (Sigma).

    Quantitation Assay:

    Article Title: Decreased miR-155-5p, miR-15a, and miR-186 Expression in Gastric Cancer Is Associated with Advanced Tumor Grade and Metastasis
    Article Snippet: .. MiRNA quantitation Briefly, a poly-A tail was added to total RNAs (500 ng) using Poly-A polymerase (New England Biolabs, UK) at 37 °C for 30 min according to manufacturer’s protocol. .. For cDNA synthesis, we used PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa, Japan) and an RT adaptor primer ( ).

    Produced:

    Article Title: Sequences in the U3 region of Human Immunodeficiency Virus 1 improve efficiency of minus strand transfer in infected cells
    Article Snippet: .. For mutant M9-m, two PCR products were produced using pairs of oligomers 1/3 and 4/2 and VENT polymerase (NewEngland Biolabs). ..

    Polymerase Chain Reaction:

    Article Title: Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿ †
    Article Snippet: .. Full-length Ago1 was amplified by standard PCR techniques using vent polymerase (New England Biolabs) and cloned between the EcoRI and BamHI restriction sites of the pFlag-CMV2 (Sigma) vector. .. MS2 fusion constructs were generated by PCR amplification of the MS2-binding domain and cloning into the HindIII restriction site of the different pFlag-CMV2 constructs.

    Article Title: Serotype-specific interactions among functional domains of dengue virus 2 nonstructural proteins (NS) 5 and NS3 are crucial for viral RNA replication
    Article Snippet: .. The synthesized viral cDNA from collected supernatants was amplified by PCR using NEBNext High Fidelity 2× polymerase (New England Biolabs) and the viral sequence-specific primers (Ref. and ) to make overlapping fragments (each ∼800 bp) that spanned the entire genome. .. PCR products were purified by agarose gel (Zymo Research), and the nucleotide sequence of each fragment was determined by GENEWIZ Inc.

    Article Title: Sequences in the U3 region of Human Immunodeficiency Virus 1 improve efficiency of minus strand transfer in infected cells
    Article Snippet: .. For mutant M9-m, two PCR products were produced using pairs of oligomers 1/3 and 4/2 and VENT polymerase (NewEngland Biolabs). ..

    Incubation:

    Article Title: Hallmarks of Hepatitis C Virus in Equine Hepacivirus
    Article Snippet: .. The poly(U) tail was added to the 3′ end of the RNA preparation using Escherichia coli poly(U) polymerase (New England BioLabs, Ipswich, MA) and was incubated for 45 min at 37°C. .. The resulting preparation was reverse transcribed by the SuperScript First-Strand Synthesis system (Life Technologies) using an oligo(dA) adapter primer (5′-TTGCGAGCACAGAATTAATACGACTCACAAAAAAAAAAAAVN-3′).

    Sequencing:

    Article Title: Serotype-specific interactions among functional domains of dengue virus 2 nonstructural proteins (NS) 5 and NS3 are crucial for viral RNA replication
    Article Snippet: .. The synthesized viral cDNA from collected supernatants was amplified by PCR using NEBNext High Fidelity 2× polymerase (New England Biolabs) and the viral sequence-specific primers (Ref. and ) to make overlapping fragments (each ∼800 bp) that spanned the entire genome. .. PCR products were purified by agarose gel (Zymo Research), and the nucleotide sequence of each fragment was determined by GENEWIZ Inc.

    Lysis:

    Article Title: An Unusual Two-Step Control of CPEB Destruction by Pin1
    Article Snippet: .. Fifty oocytes [some injected with 10 ng of mRNA synthesized with an Ambion mMESSAGE kit and polyadenylated in vitro with Escherichia coli poly(A) polymerase (New England BioLabs)] or HEK293T cells from one 10-cm dish were homogenized in lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% deoxycholate, 0.1% SDS, 50 mM Tris-HCl (pH 7.7), 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM dithiothreitol, and protease inhibitor cocktail (Roche). .. The lysis buffer in some experiments also contained 10 μg/ml RNase A (Sigma).

    Injection:

    Article Title: An Unusual Two-Step Control of CPEB Destruction by Pin1
    Article Snippet: .. Fifty oocytes [some injected with 10 ng of mRNA synthesized with an Ambion mMESSAGE kit and polyadenylated in vitro with Escherichia coli poly(A) polymerase (New England BioLabs)] or HEK293T cells from one 10-cm dish were homogenized in lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% deoxycholate, 0.1% SDS, 50 mM Tris-HCl (pH 7.7), 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM dithiothreitol, and protease inhibitor cocktail (Roche). .. The lysis buffer in some experiments also contained 10 μg/ml RNase A (Sigma).

    Plasmid Preparation:

    Article Title: Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins ▿ †
    Article Snippet: .. Full-length Ago1 was amplified by standard PCR techniques using vent polymerase (New England Biolabs) and cloned between the EcoRI and BamHI restriction sites of the pFlag-CMV2 (Sigma) vector. .. MS2 fusion constructs were generated by PCR amplification of the MS2-binding domain and cloning into the HindIII restriction site of the different pFlag-CMV2 constructs.

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  • 90
    New England Biolabs polyu
    Identification of <t>Ll.LtrB</t> 3′ ends in vivo . Free intron 3′ ends were amplified by RT-PCR from L. lactis total RNA extracts. ( A ) Intron 3′ ends were identified by first extending the intron RNA with a polyA tail followed by the synthesis of a cDNA with an oligo dT. The RNA strand was removed by an RNAse H treatment and the single strand DNA amplified by PCR. ( B ) The PCR reactions were ran on a 2% agarose gel and the chromatogram of some of the sequenced bands are shown ( C – D ). The same procedure was repeated for the Ll.LtrB-ΔA construct but extending a <t>polyU</t> instead of a polyA tail at the 3′ end of the intron ( E ).
    Polyu, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyu/product/New England Biolabs
    Average 90 stars, based on 112 article reviews
    Price from $9.99 to $1999.99
    polyu - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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    Identification of Ll.LtrB 3′ ends in vivo . Free intron 3′ ends were amplified by RT-PCR from L. lactis total RNA extracts. ( A ) Intron 3′ ends were identified by first extending the intron RNA with a polyA tail followed by the synthesis of a cDNA with an oligo dT. The RNA strand was removed by an RNAse H treatment and the single strand DNA amplified by PCR. ( B ) The PCR reactions were ran on a 2% agarose gel and the chromatogram of some of the sequenced bands are shown ( C – D ). The same procedure was repeated for the Ll.LtrB-ΔA construct but extending a polyU instead of a polyA tail at the 3′ end of the intron ( E ).

    Journal: Nucleic Acids Research

    Article Title: Circularization pathway of a bacterial group II intron

    doi: 10.1093/nar/gkv1381

    Figure Lengend Snippet: Identification of Ll.LtrB 3′ ends in vivo . Free intron 3′ ends were amplified by RT-PCR from L. lactis total RNA extracts. ( A ) Intron 3′ ends were identified by first extending the intron RNA with a polyA tail followed by the synthesis of a cDNA with an oligo dT. The RNA strand was removed by an RNAse H treatment and the single strand DNA amplified by PCR. ( B ) The PCR reactions were ran on a 2% agarose gel and the chromatogram of some of the sequenced bands are shown ( C – D ). The same procedure was repeated for the Ll.LtrB-ΔA construct but extending a polyU instead of a polyA tail at the 3′ end of the intron ( E ).

    Article Snippet: For Ll.LtrB-ΔA a second amplification of the 3′ end was performed where a polyU instead of a polyA tail was added using 2U of Poly(U) Polymerase (New England Biolabs) (10 min at 37°C).

    Techniques: In Vivo, Amplification, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Construct