deep vent dna polymerase  (New England Biolabs)


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    New England Biolabs deep vent dna polymerase
    Deep Vent Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    deep vent dna polymerase  (New England Biolabs)


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    New England Biolabs deep vent dna polymerase
    Deep Vent Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
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    deep vent dna polymerases  (New England Biolabs)


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    New England Biolabs deep vent dna polymerases
    Deep Vent Dna Polymerases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    deep vent dna polymerase  (New England Biolabs)


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    New England Biolabs deep vent dna polymerase
    Deep Vent Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deep vent dna polymerase/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
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    deep vent polymerase  (New England Biolabs)


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    New England Biolabs deep vent polymerase
    Deep Vent Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
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    deep vent dna polymerase  (New England Biolabs)


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    New England Biolabs deep vent dna polymerase
    Evaluation of the stop primer. (a) The design of the stop primer and the way of ligation using the stop primer. Three consecutive o -nitrobenzyl modifications were introduced at the 5′ side of the primer region. An annealing region was provided after a 9 nucleotide gap. When a PCR using a stop primer and photo-irradiation are performed, natural sticky ends are formed. After the sticky ends are annealed with others, chain extension is performed by <t>DNA</t> <t>polymerase,</t> and ligation is performed by DNA ligase. (b) Confirmation of sticky end formation. XhoI-treated PCR products were synthesized using a stop primer with or without photoirradiation. The sample was analyzed by 16% native PAGE. (c) Ligation using a stop primer or Golden Gate assembly. Lanes (A)–(E): the sample using a stop primer (A)–(C): (50 nt sticky ends, D, E: 20 nt sticky ends). Lanes F and G: the sample using Golden Gate assembly. Lanes A, D, and F: the sample was only hybridized. Lane B: only ligase was added to the sample. Lanes C and E: the samples in which chain extension and ligation were performed. Lane G: the sample in which Golden Gate assembly was performed. All samples were treated with Klenow fragments after hybridization or ligation. The ligation yields were calculated using the band intensity.
    Deep Vent Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
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    deep vent dna polymerase - by Bioz Stars, 2024-05
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    1) Product Images from "Development of PCR primers enabling the design of flexible sticky ends for efficient concatenation of long DNA fragments"

    Article Title: Development of PCR primers enabling the design of flexible sticky ends for efficient concatenation of long DNA fragments

    Journal: RSC Chemical Biology

    doi: 10.1039/d3cb00212h

    Evaluation of the stop primer. (a) The design of the stop primer and the way of ligation using the stop primer. Three consecutive o -nitrobenzyl modifications were introduced at the 5′ side of the primer region. An annealing region was provided after a 9 nucleotide gap. When a PCR using a stop primer and photo-irradiation are performed, natural sticky ends are formed. After the sticky ends are annealed with others, chain extension is performed by DNA polymerase, and ligation is performed by DNA ligase. (b) Confirmation of sticky end formation. XhoI-treated PCR products were synthesized using a stop primer with or without photoirradiation. The sample was analyzed by 16% native PAGE. (c) Ligation using a stop primer or Golden Gate assembly. Lanes (A)–(E): the sample using a stop primer (A)–(C): (50 nt sticky ends, D, E: 20 nt sticky ends). Lanes F and G: the sample using Golden Gate assembly. Lanes A, D, and F: the sample was only hybridized. Lane B: only ligase was added to the sample. Lanes C and E: the samples in which chain extension and ligation were performed. Lane G: the sample in which Golden Gate assembly was performed. All samples were treated with Klenow fragments after hybridization or ligation. The ligation yields were calculated using the band intensity.
    Figure Legend Snippet: Evaluation of the stop primer. (a) The design of the stop primer and the way of ligation using the stop primer. Three consecutive o -nitrobenzyl modifications were introduced at the 5′ side of the primer region. An annealing region was provided after a 9 nucleotide gap. When a PCR using a stop primer and photo-irradiation are performed, natural sticky ends are formed. After the sticky ends are annealed with others, chain extension is performed by DNA polymerase, and ligation is performed by DNA ligase. (b) Confirmation of sticky end formation. XhoI-treated PCR products were synthesized using a stop primer with or without photoirradiation. The sample was analyzed by 16% native PAGE. (c) Ligation using a stop primer or Golden Gate assembly. Lanes (A)–(E): the sample using a stop primer (A)–(C): (50 nt sticky ends, D, E: 20 nt sticky ends). Lanes F and G: the sample using Golden Gate assembly. Lanes A, D, and F: the sample was only hybridized. Lane B: only ligase was added to the sample. Lanes C and E: the samples in which chain extension and ligation were performed. Lane G: the sample in which Golden Gate assembly was performed. All samples were treated with Klenow fragments after hybridization or ligation. The ligation yields were calculated using the band intensity.

    Techniques Used: Ligation, Irradiation, Synthesized, Clear Native PAGE, Hybridization

    deep vent dna polymerase  (New England Biolabs)


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    New England Biolabs deep vent dna polymerase
    Deep Vent Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    deep vent dna polymerases  (New England Biolabs)


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    New England Biolabs deep vent dna polymerases
    Deep Vent Dna Polymerases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    deepvent dna polymerase  (New England Biolabs)


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    New England Biolabs deepvent dna polymerase
    Deepvent Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pyrococcus gb d pgb d dna polymerase  (New England Biolabs)


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    New England Biolabs pyrococcus gb d pgb d dna polymerase
    Pyrococcus Gb D Pgb D Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    deep vent dna polymerase  (New England Biolabs)


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    New England Biolabs deep vent dna polymerase
    Deep Vent Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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