formamidopyrimidine dna glycosylase  (New England Biolabs)


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    New England Biolabs formamidopyrimidine dna glycosylase
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    formamidopyrimidine dna glycosylase  (New England Biolabs)


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    New England Biolabs formamidopyrimidine dna glycosylase
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    fpg enzyme  (New England Biolabs)


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    New England Biolabs fpg enzyme
    <t>Oxidative</t> <t>DNA</t> damage at telomeres in TERT/TERC mutants. Oxidative DNA damage at telomeres was analyzed by qPCR in VA-13 cells transfected with pBABE empty vector (transfection control), pBABE-WT TERT/TERC (WT telomerase) or the different TERT (panel A) and TERC (panel B) mutants. Potassium bromate (KBrO 3 ) was used as a positive control. The differences in PCR kinetics (Ct) between <t>FPG-digested</t> vs undigested DNA are represented for each sample as ΔCt Telomere (FPG/Buffer) and normalized to pBABE-WT TERT/TERC . Colors of the bars represent the different gene domains in which the mutations are found: yellow (controls: pBABE and pBABE-WT TERT/TERC ), light green (TEN: p.Leu55Gln to p.Ala202Thr), light blue (RT: p.Val694Met to p.Arg865Cys), orange (CTE: p.Val1090Met and p.Thr1101Met) for TERT and pink (P1a stem: n.23G>C), purple (t/PK: n.96_97delCT and n.98G>A), dark blue (CR4/5: n.269G>A and n.325G>T for TERC . Graph bars represent mean values ± SEM of eight technical replicates from two independent experiments. Statistical significance between pBABE-WT TERT/TERC and each TERT or TERC mutant was calculated using two-tailed unpaired t-test ( * P < 0.05; * * P < 0.01; * * * P < 0.001).
    Fpg Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Clinical mutations in the TERT and TERC genes coding for telomerase components induced oxidative stress, DNA damage at telomeres and cell apoptosis besides decreased telomerase activity"

    Article Title: Clinical mutations in the TERT and TERC genes coding for telomerase components induced oxidative stress, DNA damage at telomeres and cell apoptosis besides decreased telomerase activity

    Journal: Human Molecular Genetics

    doi: 10.1093/hmg/ddae015

    Oxidative DNA damage at telomeres in TERT/TERC mutants. Oxidative DNA damage at telomeres was analyzed by qPCR in VA-13 cells transfected with pBABE empty vector (transfection control), pBABE-WT TERT/TERC (WT telomerase) or the different TERT (panel A) and TERC (panel B) mutants. Potassium bromate (KBrO 3 ) was used as a positive control. The differences in PCR kinetics (Ct) between FPG-digested vs undigested DNA are represented for each sample as ΔCt Telomere (FPG/Buffer) and normalized to pBABE-WT TERT/TERC . Colors of the bars represent the different gene domains in which the mutations are found: yellow (controls: pBABE and pBABE-WT TERT/TERC ), light green (TEN: p.Leu55Gln to p.Ala202Thr), light blue (RT: p.Val694Met to p.Arg865Cys), orange (CTE: p.Val1090Met and p.Thr1101Met) for TERT and pink (P1a stem: n.23G>C), purple (t/PK: n.96_97delCT and n.98G>A), dark blue (CR4/5: n.269G>A and n.325G>T for TERC . Graph bars represent mean values ± SEM of eight technical replicates from two independent experiments. Statistical significance between pBABE-WT TERT/TERC and each TERT or TERC mutant was calculated using two-tailed unpaired t-test ( * P < 0.05; * * P < 0.01; * * * P < 0.001).
    Figure Legend Snippet: Oxidative DNA damage at telomeres in TERT/TERC mutants. Oxidative DNA damage at telomeres was analyzed by qPCR in VA-13 cells transfected with pBABE empty vector (transfection control), pBABE-WT TERT/TERC (WT telomerase) or the different TERT (panel A) and TERC (panel B) mutants. Potassium bromate (KBrO 3 ) was used as a positive control. The differences in PCR kinetics (Ct) between FPG-digested vs undigested DNA are represented for each sample as ΔCt Telomere (FPG/Buffer) and normalized to pBABE-WT TERT/TERC . Colors of the bars represent the different gene domains in which the mutations are found: yellow (controls: pBABE and pBABE-WT TERT/TERC ), light green (TEN: p.Leu55Gln to p.Ala202Thr), light blue (RT: p.Val694Met to p.Arg865Cys), orange (CTE: p.Val1090Met and p.Thr1101Met) for TERT and pink (P1a stem: n.23G>C), purple (t/PK: n.96_97delCT and n.98G>A), dark blue (CR4/5: n.269G>A and n.325G>T for TERC . Graph bars represent mean values ± SEM of eight technical replicates from two independent experiments. Statistical significance between pBABE-WT TERT/TERC and each TERT or TERC mutant was calculated using two-tailed unpaired t-test ( * P < 0.05; * * P < 0.01; * * * P < 0.001).

    Techniques Used: Transfection, Plasmid Preparation, Positive Control, Mutagenesis, Two Tailed Test

    fpg enzyme  (New England Biolabs)


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    fpg enzyme  (New England Biolabs)


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    fpg  (New England Biolabs)


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    formamidopyrimidine glycosylase fpg  (New England Biolabs)


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    fpg  (New England Biolabs)


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    fpg neb cat  (New England Biolabs)


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    New England Biolabs fpg neb cat
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    fpg  (New England Biolabs)


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    New England Biolabs formamidopyrimidine dna glycosylase
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    New England Biolabs fpg enzyme
    <t>Oxidative</t> <t>DNA</t> damage at telomeres in TERT/TERC mutants. Oxidative DNA damage at telomeres was analyzed by qPCR in VA-13 cells transfected with pBABE empty vector (transfection control), pBABE-WT TERT/TERC (WT telomerase) or the different TERT (panel A) and TERC (panel B) mutants. Potassium bromate (KBrO 3 ) was used as a positive control. The differences in PCR kinetics (Ct) between <t>FPG-digested</t> vs undigested DNA are represented for each sample as ΔCt Telomere (FPG/Buffer) and normalized to pBABE-WT TERT/TERC . Colors of the bars represent the different gene domains in which the mutations are found: yellow (controls: pBABE and pBABE-WT TERT/TERC ), light green (TEN: p.Leu55Gln to p.Ala202Thr), light blue (RT: p.Val694Met to p.Arg865Cys), orange (CTE: p.Val1090Met and p.Thr1101Met) for TERT and pink (P1a stem: n.23G>C), purple (t/PK: n.96_97delCT and n.98G>A), dark blue (CR4/5: n.269G>A and n.325G>T for TERC . Graph bars represent mean values ± SEM of eight technical replicates from two independent experiments. Statistical significance between pBABE-WT TERT/TERC and each TERT or TERC mutant was calculated using two-tailed unpaired t-test ( * P < 0.05; * * P < 0.01; * * * P < 0.001).
    Fpg Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    <t>Oxidative</t> <t>DNA</t> damage at telomeres in TERT/TERC mutants. Oxidative DNA damage at telomeres was analyzed by qPCR in VA-13 cells transfected with pBABE empty vector (transfection control), pBABE-WT TERT/TERC (WT telomerase) or the different TERT (panel A) and TERC (panel B) mutants. Potassium bromate (KBrO 3 ) was used as a positive control. The differences in PCR kinetics (Ct) between <t>FPG-digested</t> vs undigested DNA are represented for each sample as ΔCt Telomere (FPG/Buffer) and normalized to pBABE-WT TERT/TERC . Colors of the bars represent the different gene domains in which the mutations are found: yellow (controls: pBABE and pBABE-WT TERT/TERC ), light green (TEN: p.Leu55Gln to p.Ala202Thr), light blue (RT: p.Val694Met to p.Arg865Cys), orange (CTE: p.Val1090Met and p.Thr1101Met) for TERT and pink (P1a stem: n.23G>C), purple (t/PK: n.96_97delCT and n.98G>A), dark blue (CR4/5: n.269G>A and n.325G>T for TERC . Graph bars represent mean values ± SEM of eight technical replicates from two independent experiments. Statistical significance between pBABE-WT TERT/TERC and each TERT or TERC mutant was calculated using two-tailed unpaired t-test ( * P < 0.05; * * P < 0.01; * * * P < 0.001).
    Fpg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    <t>Oxidative</t> <t>DNA</t> damage at telomeres in TERT/TERC mutants. Oxidative DNA damage at telomeres was analyzed by qPCR in VA-13 cells transfected with pBABE empty vector (transfection control), pBABE-WT TERT/TERC (WT telomerase) or the different TERT (panel A) and TERC (panel B) mutants. Potassium bromate (KBrO 3 ) was used as a positive control. The differences in PCR kinetics (Ct) between <t>FPG-digested</t> vs undigested DNA are represented for each sample as ΔCt Telomere (FPG/Buffer) and normalized to pBABE-WT TERT/TERC . Colors of the bars represent the different gene domains in which the mutations are found: yellow (controls: pBABE and pBABE-WT TERT/TERC ), light green (TEN: p.Leu55Gln to p.Ala202Thr), light blue (RT: p.Val694Met to p.Arg865Cys), orange (CTE: p.Val1090Met and p.Thr1101Met) for TERT and pink (P1a stem: n.23G>C), purple (t/PK: n.96_97delCT and n.98G>A), dark blue (CR4/5: n.269G>A and n.325G>T for TERC . Graph bars represent mean values ± SEM of eight technical replicates from two independent experiments. Statistical significance between pBABE-WT TERT/TERC and each TERT or TERC mutant was calculated using two-tailed unpaired t-test ( * P < 0.05; * * P < 0.01; * * * P < 0.001).
    Formamidopyrimidine Glycosylase Fpg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs fpg neb cat
    <t>Oxidative</t> <t>DNA</t> damage at telomeres in TERT/TERC mutants. Oxidative DNA damage at telomeres was analyzed by qPCR in VA-13 cells transfected with pBABE empty vector (transfection control), pBABE-WT TERT/TERC (WT telomerase) or the different TERT (panel A) and TERC (panel B) mutants. Potassium bromate (KBrO 3 ) was used as a positive control. The differences in PCR kinetics (Ct) between <t>FPG-digested</t> vs undigested DNA are represented for each sample as ΔCt Telomere (FPG/Buffer) and normalized to pBABE-WT TERT/TERC . Colors of the bars represent the different gene domains in which the mutations are found: yellow (controls: pBABE and pBABE-WT TERT/TERC ), light green (TEN: p.Leu55Gln to p.Ala202Thr), light blue (RT: p.Val694Met to p.Arg865Cys), orange (CTE: p.Val1090Met and p.Thr1101Met) for TERT and pink (P1a stem: n.23G>C), purple (t/PK: n.96_97delCT and n.98G>A), dark blue (CR4/5: n.269G>A and n.325G>T for TERC . Graph bars represent mean values ± SEM of eight technical replicates from two independent experiments. Statistical significance between pBABE-WT TERT/TERC and each TERT or TERC mutant was calculated using two-tailed unpaired t-test ( * P < 0.05; * * P < 0.01; * * * P < 0.001).
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    Oxidative DNA damage at telomeres in TERT/TERC mutants. Oxidative DNA damage at telomeres was analyzed by qPCR in VA-13 cells transfected with pBABE empty vector (transfection control), pBABE-WT TERT/TERC (WT telomerase) or the different TERT (panel A) and TERC (panel B) mutants. Potassium bromate (KBrO 3 ) was used as a positive control. The differences in PCR kinetics (Ct) between FPG-digested vs undigested DNA are represented for each sample as ΔCt Telomere (FPG/Buffer) and normalized to pBABE-WT TERT/TERC . Colors of the bars represent the different gene domains in which the mutations are found: yellow (controls: pBABE and pBABE-WT TERT/TERC ), light green (TEN: p.Leu55Gln to p.Ala202Thr), light blue (RT: p.Val694Met to p.Arg865Cys), orange (CTE: p.Val1090Met and p.Thr1101Met) for TERT and pink (P1a stem: n.23G>C), purple (t/PK: n.96_97delCT and n.98G>A), dark blue (CR4/5: n.269G>A and n.325G>T for TERC . Graph bars represent mean values ± SEM of eight technical replicates from two independent experiments. Statistical significance between pBABE-WT TERT/TERC and each TERT or TERC mutant was calculated using two-tailed unpaired t-test ( * P < 0.05; * * P < 0.01; * * * P < 0.001).

    Journal: Human Molecular Genetics

    Article Title: Clinical mutations in the TERT and TERC genes coding for telomerase components induced oxidative stress, DNA damage at telomeres and cell apoptosis besides decreased telomerase activity

    doi: 10.1093/hmg/ddae015

    Figure Lengend Snippet: Oxidative DNA damage at telomeres in TERT/TERC mutants. Oxidative DNA damage at telomeres was analyzed by qPCR in VA-13 cells transfected with pBABE empty vector (transfection control), pBABE-WT TERT/TERC (WT telomerase) or the different TERT (panel A) and TERC (panel B) mutants. Potassium bromate (KBrO 3 ) was used as a positive control. The differences in PCR kinetics (Ct) between FPG-digested vs undigested DNA are represented for each sample as ΔCt Telomere (FPG/Buffer) and normalized to pBABE-WT TERT/TERC . Colors of the bars represent the different gene domains in which the mutations are found: yellow (controls: pBABE and pBABE-WT TERT/TERC ), light green (TEN: p.Leu55Gln to p.Ala202Thr), light blue (RT: p.Val694Met to p.Arg865Cys), orange (CTE: p.Val1090Met and p.Thr1101Met) for TERT and pink (P1a stem: n.23G>C), purple (t/PK: n.96_97delCT and n.98G>A), dark blue (CR4/5: n.269G>A and n.325G>T for TERC . Graph bars represent mean values ± SEM of eight technical replicates from two independent experiments. Statistical significance between pBABE-WT TERT/TERC and each TERT or TERC mutant was calculated using two-tailed unpaired t-test ( * P < 0.05; * * P < 0.01; * * * P < 0.001).

    Article Snippet: We have adapted the telomere oxidation protocol previously described [ ] to quantify the relative accumulation of oxidized bases at specific genomic regions by incubating the DNA with the FPG enzyme from NEB (M0240; New England Biolabs, Ipswich, MA, USA) as previously reported.

    Techniques: Transfection, Plasmid Preparation, Positive Control, Mutagenesis, Two Tailed Test