m0222l  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    New England Biolabs m0222l
    M0222l, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m0222l/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m0222l - by Bioz Stars, 2024-05
    93/100 stars

    Images

    m0222l  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    New England Biolabs m0222l
    M0222l, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m0222l/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m0222l - by Bioz Stars, 2024-05
    93/100 stars

    Images

    dna adenine methyltransferase  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    New England Biolabs dna adenine methyltransferase
    Dna Adenine Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna adenine methyltransferase/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna adenine methyltransferase - by Bioz Stars, 2024-05
    93/100 stars

    Images

    dam methyltransferase reaction buffer  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    New England Biolabs dam methyltransferase reaction buffer
    Dam Methyltransferase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dam methyltransferase reaction buffer/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dam methyltransferase reaction buffer - by Bioz Stars, 2024-05
    93/100 stars

    Images

    e coli dam methyltransferase  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    New England Biolabs e coli dam methyltransferase
    E Coli Dam Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli dam methyltransferase/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli dam methyltransferase - by Bioz Stars, 2024-05
    93/100 stars

    Images

    m0222l  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    New England Biolabs m0222l
    M0222l, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m0222l/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m0222l - by Bioz Stars, 2024-05
    93/100 stars

    Images

    1x dam methyltransferase buffer  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    New England Biolabs 1x dam methyltransferase buffer
    R-M systems associated with S. enterica LT2. MTases in gray are considered orphan MTases that are not associated with REases. b, Number of <t>MTase</t> sites present in the two tested plasmids. c, Electroporation efficiency of plasmids pJV400 (top) and pJV414 (bottom) subjected to IMPRINT with different MTase combinations into S. enterica LT2. All transformation efficiencies are normalized to that of unmethylated DNA. Error bars represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05. HM: host-methylated plasmid DNA extracted from E. coli MG1655 and subjected to IMPRINT without any added MTases.
    1x Dam Methyltransferase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x dam methyltransferase buffer/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x dam methyltransferase buffer - by Bioz Stars, 2024-05
    93/100 stars

    Images

    1) Product Images from "A cell-free transcription-translation pipeline for recreating methylation patterns boosts DNA transformation in bacteria"

    Article Title: A cell-free transcription-translation pipeline for recreating methylation patterns boosts DNA transformation in bacteria

    Journal: bioRxiv

    doi: 10.1101/2023.09.16.557782

    R-M systems associated with S. enterica LT2. MTases in gray are considered orphan MTases that are not associated with REases. b, Number of MTase sites present in the two tested plasmids. c, Electroporation efficiency of plasmids pJV400 (top) and pJV414 (bottom) subjected to IMPRINT with different MTase combinations into S. enterica LT2. All transformation efficiencies are normalized to that of unmethylated DNA. Error bars represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05. HM: host-methylated plasmid DNA extracted from E. coli MG1655 and subjected to IMPRINT without any added MTases.
    Figure Legend Snippet: R-M systems associated with S. enterica LT2. MTases in gray are considered orphan MTases that are not associated with REases. b, Number of MTase sites present in the two tested plasmids. c, Electroporation efficiency of plasmids pJV400 (top) and pJV414 (bottom) subjected to IMPRINT with different MTase combinations into S. enterica LT2. All transformation efficiencies are normalized to that of unmethylated DNA. Error bars represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05. HM: host-methylated plasmid DNA extracted from E. coli MG1655 and subjected to IMPRINT without any added MTases.

    Techniques Used: Electroporation, Transformation Assay, Standard Deviation, Methylation, Plasmid Preparation

    Conserved Type IA R-M methyltransferase mutations promote methylation of unmethylated DNA to achieve near-complete protection in S. enterica LT2. a, Aligned type IA MTases between E. coli and S. enterica . Mutations previously shown to enable efficient methylation of unmethylated DNA are shown in red. IA MTases normally prefer hemimethylated DNA as substrates. b, Restriction digestion of plasmid pJV412 methylated using IMPRINT. A HinFI restriction site was inserted to be methylated through one of the MS.SenLT2II recognition sites. HinFI digests the plasmid in six other locations, explaining the consistent lower bands on the gel. c, Electroporation efficiency of plasmid pJV400 subjected to IMPRINT with different MTase combinations into S. enterica LT2. All transformation efficiencies are normalized to that of unmethylated DNA. Error bars represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05. HM: host-methylated plasmid DNA extracted from E. coli MG1655 and subjected to IMPRINT without any added MTases.
    Figure Legend Snippet: Conserved Type IA R-M methyltransferase mutations promote methylation of unmethylated DNA to achieve near-complete protection in S. enterica LT2. a, Aligned type IA MTases between E. coli and S. enterica . Mutations previously shown to enable efficient methylation of unmethylated DNA are shown in red. IA MTases normally prefer hemimethylated DNA as substrates. b, Restriction digestion of plasmid pJV412 methylated using IMPRINT. A HinFI restriction site was inserted to be methylated through one of the MS.SenLT2II recognition sites. HinFI digests the plasmid in six other locations, explaining the consistent lower bands on the gel. c, Electroporation efficiency of plasmid pJV400 subjected to IMPRINT with different MTase combinations into S. enterica LT2. All transformation efficiencies are normalized to that of unmethylated DNA. Error bars represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05. HM: host-methylated plasmid DNA extracted from E. coli MG1655 and subjected to IMPRINT without any added MTases.

    Techniques Used: Methylation, Plasmid Preparation, Electroporation, Transformation Assay, Standard Deviation

    Overview of High-throughput IMPRINT (HT-IMPRINT). Barcodes are associated with each MTase combination to track how that combination impacts the transformation efficiency. b, R-M systems associated with B. breve UCC2003. c, Heat map of the relative impact of different MTase combinations using HT-IMPRINT with pJV420 in B. breve UCC2003. Three barcodes were associated with each MTase combination, where the reported values are the median relative abundance normalized to the untransformed library. d, Electroporation efficiency of plasmid pJV420 subjected to IMPRINT with different MTase combinations into B. breve UCC2003. e, R-M systems associated with B. longum ATCC 15707. f, Heat map of the relative impact of different MTase combinations using HT-IMPRINT with pJV420 in B. longum ATCC 15707. Two barcodes were associated with each MTase combination, where the reported values are the number of colonies possessing a barcode associated with the indicated MTase combination. g, Electroporation efficiency of plasmid pJV420 subjected to IMPRINT with different MTase combinations into B. longum ATCC 15707.
    Figure Legend Snippet: Overview of High-throughput IMPRINT (HT-IMPRINT). Barcodes are associated with each MTase combination to track how that combination impacts the transformation efficiency. b, R-M systems associated with B. breve UCC2003. c, Heat map of the relative impact of different MTase combinations using HT-IMPRINT with pJV420 in B. breve UCC2003. Three barcodes were associated with each MTase combination, where the reported values are the median relative abundance normalized to the untransformed library. d, Electroporation efficiency of plasmid pJV420 subjected to IMPRINT with different MTase combinations into B. breve UCC2003. e, R-M systems associated with B. longum ATCC 15707. f, Heat map of the relative impact of different MTase combinations using HT-IMPRINT with pJV420 in B. longum ATCC 15707. Two barcodes were associated with each MTase combination, where the reported values are the number of colonies possessing a barcode associated with the indicated MTase combination. g, Electroporation efficiency of plasmid pJV420 subjected to IMPRINT with different MTase combinations into B. longum ATCC 15707.

    Techniques Used: High Throughput Screening Assay, Transformation Assay, Electroporation, Plasmid Preparation

    Compatibility between optimal MTase combinations and transformation efficiency across bacterial strains. The plasmid pJV420 was methylated with optimal patterns for each strain using IMPRINT and transformed into the different strains. E. coli EC135 lacks any R-M systems and thus serves as a transformation control. b, Electroporation efficiency of pJV420 with the different methylation patterns into the different bacterial strains. All transformation efficiencies for a given strain are normalized to that of unmethylated DNA. Heat maps represent the geometric mean of triplicate transformations. See Fig. S7 for individual measurements. Error bars in d and g represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05.
    Figure Legend Snippet: Compatibility between optimal MTase combinations and transformation efficiency across bacterial strains. The plasmid pJV420 was methylated with optimal patterns for each strain using IMPRINT and transformed into the different strains. E. coli EC135 lacks any R-M systems and thus serves as a transformation control. b, Electroporation efficiency of pJV420 with the different methylation patterns into the different bacterial strains. All transformation efficiencies for a given strain are normalized to that of unmethylated DNA. Heat maps represent the geometric mean of triplicate transformations. See Fig. S7 for individual measurements. Error bars in d and g represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05.

    Techniques Used: Transformation Assay, Plasmid Preparation, Methylation, Electroporation, Standard Deviation

    methyltransferase  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    New England Biolabs methyltransferase
    Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/methyltransferase/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    methyltransferase - by Bioz Stars, 2024-05
    93/100 stars

    Images

    dam methyltransferase  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    New England Biolabs dam methyltransferase
    Dam Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dam methyltransferase/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dam methyltransferase - by Bioz Stars, 2024-05
    93/100 stars

    Images

    dam methyltransferase  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    New England Biolabs dam methyltransferase
    Dam Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dam methyltransferase/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dam methyltransferase - by Bioz Stars, 2024-05
    93/100 stars

    Images

    dam gatc  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    New England Biolabs dam gatc
    Dam Gatc, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dam gatc/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dam gatc - by Bioz Stars, 2024-05
    93/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    New England Biolabs m0222l
    M0222l, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m0222l/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m0222l - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    New England Biolabs dna adenine methyltransferase
    Dna Adenine Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna adenine methyltransferase/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna adenine methyltransferase - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    New England Biolabs dam methyltransferase reaction buffer
    Dam Methyltransferase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dam methyltransferase reaction buffer/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dam methyltransferase reaction buffer - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    New England Biolabs e coli dam methyltransferase
    E Coli Dam Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli dam methyltransferase/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli dam methyltransferase - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    New England Biolabs 1x dam methyltransferase buffer
    R-M systems associated with S. enterica LT2. MTases in gray are considered orphan MTases that are not associated with REases. b, Number of <t>MTase</t> sites present in the two tested plasmids. c, Electroporation efficiency of plasmids pJV400 (top) and pJV414 (bottom) subjected to IMPRINT with different MTase combinations into S. enterica LT2. All transformation efficiencies are normalized to that of unmethylated DNA. Error bars represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05. HM: host-methylated plasmid DNA extracted from E. coli MG1655 and subjected to IMPRINT without any added MTases.
    1x Dam Methyltransferase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x dam methyltransferase buffer/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x dam methyltransferase buffer - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    New England Biolabs methyltransferase
    R-M systems associated with S. enterica LT2. MTases in gray are considered orphan MTases that are not associated with REases. b, Number of <t>MTase</t> sites present in the two tested plasmids. c, Electroporation efficiency of plasmids pJV400 (top) and pJV414 (bottom) subjected to IMPRINT with different MTase combinations into S. enterica LT2. All transformation efficiencies are normalized to that of unmethylated DNA. Error bars represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05. HM: host-methylated plasmid DNA extracted from E. coli MG1655 and subjected to IMPRINT without any added MTases.
    Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/methyltransferase/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    methyltransferase - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    New England Biolabs dam methyltransferase
    R-M systems associated with S. enterica LT2. MTases in gray are considered orphan MTases that are not associated with REases. b, Number of <t>MTase</t> sites present in the two tested plasmids. c, Electroporation efficiency of plasmids pJV400 (top) and pJV414 (bottom) subjected to IMPRINT with different MTase combinations into S. enterica LT2. All transformation efficiencies are normalized to that of unmethylated DNA. Error bars represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05. HM: host-methylated plasmid DNA extracted from E. coli MG1655 and subjected to IMPRINT without any added MTases.
    Dam Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dam methyltransferase/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dam methyltransferase - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    New England Biolabs dam gatc
    R-M systems associated with S. enterica LT2. MTases in gray are considered orphan MTases that are not associated with REases. b, Number of <t>MTase</t> sites present in the two tested plasmids. c, Electroporation efficiency of plasmids pJV400 (top) and pJV414 (bottom) subjected to IMPRINT with different MTase combinations into S. enterica LT2. All transformation efficiencies are normalized to that of unmethylated DNA. Error bars represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05. HM: host-methylated plasmid DNA extracted from E. coli MG1655 and subjected to IMPRINT without any added MTases.
    Dam Gatc, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dam gatc/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dam gatc - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    R-M systems associated with S. enterica LT2. MTases in gray are considered orphan MTases that are not associated with REases. b, Number of MTase sites present in the two tested plasmids. c, Electroporation efficiency of plasmids pJV400 (top) and pJV414 (bottom) subjected to IMPRINT with different MTase combinations into S. enterica LT2. All transformation efficiencies are normalized to that of unmethylated DNA. Error bars represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05. HM: host-methylated plasmid DNA extracted from E. coli MG1655 and subjected to IMPRINT without any added MTases.

    Journal: bioRxiv

    Article Title: A cell-free transcription-translation pipeline for recreating methylation patterns boosts DNA transformation in bacteria

    doi: 10.1101/2023.09.16.557782

    Figure Lengend Snippet: R-M systems associated with S. enterica LT2. MTases in gray are considered orphan MTases that are not associated with REases. b, Number of MTase sites present in the two tested plasmids. c, Electroporation efficiency of plasmids pJV400 (top) and pJV414 (bottom) subjected to IMPRINT with different MTase combinations into S. enterica LT2. All transformation efficiencies are normalized to that of unmethylated DNA. Error bars represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05. HM: host-methylated plasmid DNA extracted from E. coli MG1655 and subjected to IMPRINT without any added MTases.

    Article Snippet: Then, a methylation reaction was set up (50 µL) by mixing a total of 1 µL of the TXTL reaction(s) with 1 - 2 μg of the shuttle vector or 1 μg of unmethylated linear DNA, 1x Dam methyltransferase buffer (NEB CN# M0222) and 640 µM AdoMet (NEB CN# B9003).

    Techniques: Electroporation, Transformation Assay, Standard Deviation, Methylation, Plasmid Preparation

    Conserved Type IA R-M methyltransferase mutations promote methylation of unmethylated DNA to achieve near-complete protection in S. enterica LT2. a, Aligned type IA MTases between E. coli and S. enterica . Mutations previously shown to enable efficient methylation of unmethylated DNA are shown in red. IA MTases normally prefer hemimethylated DNA as substrates. b, Restriction digestion of plasmid pJV412 methylated using IMPRINT. A HinFI restriction site was inserted to be methylated through one of the MS.SenLT2II recognition sites. HinFI digests the plasmid in six other locations, explaining the consistent lower bands on the gel. c, Electroporation efficiency of plasmid pJV400 subjected to IMPRINT with different MTase combinations into S. enterica LT2. All transformation efficiencies are normalized to that of unmethylated DNA. Error bars represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05. HM: host-methylated plasmid DNA extracted from E. coli MG1655 and subjected to IMPRINT without any added MTases.

    Journal: bioRxiv

    Article Title: A cell-free transcription-translation pipeline for recreating methylation patterns boosts DNA transformation in bacteria

    doi: 10.1101/2023.09.16.557782

    Figure Lengend Snippet: Conserved Type IA R-M methyltransferase mutations promote methylation of unmethylated DNA to achieve near-complete protection in S. enterica LT2. a, Aligned type IA MTases between E. coli and S. enterica . Mutations previously shown to enable efficient methylation of unmethylated DNA are shown in red. IA MTases normally prefer hemimethylated DNA as substrates. b, Restriction digestion of plasmid pJV412 methylated using IMPRINT. A HinFI restriction site was inserted to be methylated through one of the MS.SenLT2II recognition sites. HinFI digests the plasmid in six other locations, explaining the consistent lower bands on the gel. c, Electroporation efficiency of plasmid pJV400 subjected to IMPRINT with different MTase combinations into S. enterica LT2. All transformation efficiencies are normalized to that of unmethylated DNA. Error bars represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05. HM: host-methylated plasmid DNA extracted from E. coli MG1655 and subjected to IMPRINT without any added MTases.

    Article Snippet: Then, a methylation reaction was set up (50 µL) by mixing a total of 1 µL of the TXTL reaction(s) with 1 - 2 μg of the shuttle vector or 1 μg of unmethylated linear DNA, 1x Dam methyltransferase buffer (NEB CN# M0222) and 640 µM AdoMet (NEB CN# B9003).

    Techniques: Methylation, Plasmid Preparation, Electroporation, Transformation Assay, Standard Deviation

    Overview of High-throughput IMPRINT (HT-IMPRINT). Barcodes are associated with each MTase combination to track how that combination impacts the transformation efficiency. b, R-M systems associated with B. breve UCC2003. c, Heat map of the relative impact of different MTase combinations using HT-IMPRINT with pJV420 in B. breve UCC2003. Three barcodes were associated with each MTase combination, where the reported values are the median relative abundance normalized to the untransformed library. d, Electroporation efficiency of plasmid pJV420 subjected to IMPRINT with different MTase combinations into B. breve UCC2003. e, R-M systems associated with B. longum ATCC 15707. f, Heat map of the relative impact of different MTase combinations using HT-IMPRINT with pJV420 in B. longum ATCC 15707. Two barcodes were associated with each MTase combination, where the reported values are the number of colonies possessing a barcode associated with the indicated MTase combination. g, Electroporation efficiency of plasmid pJV420 subjected to IMPRINT with different MTase combinations into B. longum ATCC 15707.

    Journal: bioRxiv

    Article Title: A cell-free transcription-translation pipeline for recreating methylation patterns boosts DNA transformation in bacteria

    doi: 10.1101/2023.09.16.557782

    Figure Lengend Snippet: Overview of High-throughput IMPRINT (HT-IMPRINT). Barcodes are associated with each MTase combination to track how that combination impacts the transformation efficiency. b, R-M systems associated with B. breve UCC2003. c, Heat map of the relative impact of different MTase combinations using HT-IMPRINT with pJV420 in B. breve UCC2003. Three barcodes were associated with each MTase combination, where the reported values are the median relative abundance normalized to the untransformed library. d, Electroporation efficiency of plasmid pJV420 subjected to IMPRINT with different MTase combinations into B. breve UCC2003. e, R-M systems associated with B. longum ATCC 15707. f, Heat map of the relative impact of different MTase combinations using HT-IMPRINT with pJV420 in B. longum ATCC 15707. Two barcodes were associated with each MTase combination, where the reported values are the number of colonies possessing a barcode associated with the indicated MTase combination. g, Electroporation efficiency of plasmid pJV420 subjected to IMPRINT with different MTase combinations into B. longum ATCC 15707.

    Article Snippet: Then, a methylation reaction was set up (50 µL) by mixing a total of 1 µL of the TXTL reaction(s) with 1 - 2 μg of the shuttle vector or 1 μg of unmethylated linear DNA, 1x Dam methyltransferase buffer (NEB CN# M0222) and 640 µM AdoMet (NEB CN# B9003).

    Techniques: High Throughput Screening Assay, Transformation Assay, Electroporation, Plasmid Preparation

    Compatibility between optimal MTase combinations and transformation efficiency across bacterial strains. The plasmid pJV420 was methylated with optimal patterns for each strain using IMPRINT and transformed into the different strains. E. coli EC135 lacks any R-M systems and thus serves as a transformation control. b, Electroporation efficiency of pJV420 with the different methylation patterns into the different bacterial strains. All transformation efficiencies for a given strain are normalized to that of unmethylated DNA. Heat maps represent the geometric mean of triplicate transformations. See Fig. S7 for individual measurements. Error bars in d and g represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05.

    Journal: bioRxiv

    Article Title: A cell-free transcription-translation pipeline for recreating methylation patterns boosts DNA transformation in bacteria

    doi: 10.1101/2023.09.16.557782

    Figure Lengend Snippet: Compatibility between optimal MTase combinations and transformation efficiency across bacterial strains. The plasmid pJV420 was methylated with optimal patterns for each strain using IMPRINT and transformed into the different strains. E. coli EC135 lacks any R-M systems and thus serves as a transformation control. b, Electroporation efficiency of pJV420 with the different methylation patterns into the different bacterial strains. All transformation efficiencies for a given strain are normalized to that of unmethylated DNA. Heat maps represent the geometric mean of triplicate transformations. See Fig. S7 for individual measurements. Error bars in d and g represent the geometric mean and standard deviation of triplicate independent experiments starting from separate TXTL reactions. The bottom of the bar marks the reference for statistical analysis. **: p < 0.01. *: p < 0.05. ns: p > 0.05.

    Article Snippet: Then, a methylation reaction was set up (50 µL) by mixing a total of 1 µL of the TXTL reaction(s) with 1 - 2 μg of the shuttle vector or 1 μg of unmethylated linear DNA, 1x Dam methyltransferase buffer (NEB CN# M0222) and 640 µM AdoMet (NEB CN# B9003).

    Techniques: Transformation Assay, Plasmid Preparation, Methylation, Electroporation, Standard Deviation