nf kb1  (Boster Bio)

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice

doi: 10.1155/2016/2715718

Figure Lengend Snippet: Effect of H 2 S on the protein level of NF- κ B in the kidney of mice. (a) The expression levels of p50, p65, and p-p65 were measured by Western blot. β -actin was used as an internal control. Bar graphs showed the quantification of p50 (b), p65 (c), p-p65 (d), and p-p65/p65 (e). Values were presented as mean ± SEM ( n = 3); ∗∗ P < 0.01 compared with the LFD group; ## P < 0.01 compared with the HFD group.

Article Snippet: After blocking, the membranes were incubated with anti-TNF- α antibody (Beyotime Institute of Biotechnology, Shanghai, China), antinuclear factor-kappa B (NF- κ B), p65 antibody (Wuhan Boster Biotech Co., Ltd., Hubei, China), anti-NF- κ B p50 antibody (Wuhan Boster Biotech Co., Ltd., Hubei, China), anti-phospho-NF- κ B p65 (Ser536) antibody (Beyotime Institute of Biotechnology, Shanghai, China), and anti- β -actin antibody (Proteintech, Hubei, China).

Techniques: Expressing, Western Blot, Control

Journal: Neoplasma

Article Title: Cellular stress response in Eca-109 cells inhibits apoptosis during early exposure to isorhamnetin.

doi: 10.4149/neo_2012_047

Figure Lengend Snippet: Figure 2. Effect of isorhamnetin on levels of NF-κB/p50, NF-κB/p65 and IκBα in Eca-109 cells. After treatment of cells with 130 µM isorhamnetin for 24, 48, 72, 96 and 120 h, cytoplasmic and nuclear proteins were isolated and analyzed by Western blot. During 0 to 72 h of treatment, nuclear levels of NF-κB/p50 and NF-κB/p65 increased dramatically, while the cytoplasmic level of IκBα decreased significantly. However, during 72 to 120 h of treatment, nuclear levels of NF-κB/p50 and NF-κB/p65 decreased and the cytoplasmic level of IκBα increased. Equal loading was confirmed by stripping the blot and reprobing for β-actin. Significant changes were determined based on three independent experiments. *p<0.05 and ** p<0.01 compared with control (0 h of treatment).

Article Snippet: Antibodies used for Western blotting were purchased from the following manufacturers: anti-p53 (sc-99), anti-Bcl-2 (sc-492), anti-Id-1 (sc-488) and anti-NF-κB/p65 (sc-8008) antibodies were from Santa Cruz Biotechnology (USA); anti-COX-2 (ZA0243), anti-Mcl1(ZA0569), anti-IκBα (ZA0509), anti-Akt(T308) (ZP0026) and anti-Akt(S473) (ZP0024) antibodies were from ABZoom (Chengdu, China); and anti-β-actin, anti-NF-κB/p50 and anti-Bax antibodies were from BOSTER (Chengdu, China).

Techniques: Isolation, Western Blot, Stripping Membranes, Control

Journal: Neoplasma

Article Title: Cellular stress response in Eca-109 cells inhibits apoptosis during early exposure to isorhamnetin.

doi: 10.4149/neo_2012_047

Figure Lengend Snippet: Figure 4. Relationship among NF-κB, p-Akt, Id-1, p53and COX-2 expression in Eca-109 cells during the first 48 h of exposure to isorhamnetin. Cells were treated with 130 µM isorhamnetin for 24 or 48 h and whole-cell extracts were analyzed by Western blot. (A) Phospho-Akt(T308)/(S473) was not detected under these conditions, whereas the level of NF-κB peaked at 48 h and the level of COX-2 increased sharply from 0 to 48 h. (B) The level of Id-1 decreased significantly from 0 to 48 h, while the nuclear levels of NF-κB/p50 and NF-κB/p65 increased. No significant change in the levels of p53 was observed. Equal loading was confirmed by stripping the blot and reprobing for β-actin. Significant changes were determined based on three independent experiments. *p<0.05 and ** p<0.01 compared with control (0 h of treatment).

Article Snippet: Antibodies used for Western blotting were purchased from the following manufacturers: anti-p53 (sc-99), anti-Bcl-2 (sc-492), anti-Id-1 (sc-488) and anti-NF-κB/p65 (sc-8008) antibodies were from Santa Cruz Biotechnology (USA); anti-COX-2 (ZA0243), anti-Mcl1(ZA0569), anti-IκBα (ZA0509), anti-Akt(T308) (ZP0026) and anti-Akt(S473) (ZP0024) antibodies were from ABZoom (Chengdu, China); and anti-β-actin, anti-NF-κB/p50 and anti-Bax antibodies were from BOSTER (Chengdu, China).

Techniques: Expressing, Western Blot, Stripping Membranes, Control

Journal: Neoplasma

Article Title: Cellular stress response in Eca-109 cells inhibits apoptosis during early exposure to isorhamnetin.

doi: 10.4149/neo_2012_047

Figure Lengend Snippet: Figure 6. Inhibition by MG132 of isorhamnetin-induced activation of NF-κB. Eca-109 cells were pretreated with MG132 or DMSO vehicle and then treated with isorhamnetin as described in Fig. 5. Nuclear levels of NF-κB/p50 and NF-κB/p65 were significantlylowerincellspretreatedwith MG132 than in those treated with isorhamnetin alone. Results shown are from three independent experiments. ** p<0.01 compared with control cells (no pretreatment)

Article Snippet: Antibodies used for Western blotting were purchased from the following manufacturers: anti-p53 (sc-99), anti-Bcl-2 (sc-492), anti-Id-1 (sc-488) and anti-NF-κB/p65 (sc-8008) antibodies were from Santa Cruz Biotechnology (USA); anti-COX-2 (ZA0243), anti-Mcl1(ZA0569), anti-IκBα (ZA0509), anti-Akt(T308) (ZP0026) and anti-Akt(S473) (ZP0024) antibodies were from ABZoom (Chengdu, China); and anti-β-actin, anti-NF-κB/p50 and anti-Bax antibodies were from BOSTER (Chengdu, China).

Techniques: Inhibition, Activation Assay, Control