n m cntf  (Alomone Labs)

Journal: The Journal of Biological Chemistry

Article Title: Identification of a Linear Epitope in Sortilin That Partakes in Pro-neurotrophin Binding *

doi: 10.1074/jbc.M109.062364

Figure Lengend Snippet: Differential binding of pro-BDNF and neurotension to sortilin and pro-sortilin. A , SPR analysis showing that unprocessed pro-BDNF (50 n m ) binds nearly as efficiently to the receptor in the presence (pro-sortilin) as in the absence (sortilin) of the receptor

Article Snippet: Internalization of bound ligands was studied by the incubation of cells for 30 min at 37 °C with anti-sortilin polyclonal antibodies (10 μg/ml), GST-NGFpro (1 μg/ml), or 100 n m BDNF pro-domain (Alomone) diluted in culture medium.

Techniques: Binding Assay, SPR Assay

Journal: The Journal of Biological Chemistry

Article Title: Identification of a Linear Epitope in Sortilin That Partakes in Pro-neurotrophin Binding *

doi: 10.1074/jbc.M109.062364

Figure Lengend Snippet: Selective competition of ligands by sortilin-derived peptide antagonist. SPR binding analysis of 50 n m unprocessed pro-BDNF ( A ), 50 n m unprocessed pro-NGF ( B ), and 90 n m RAP ( C ) to immobilized sortilin in the absence and presence of the sort166–181

Article Snippet: Internalization of bound ligands was studied by the incubation of cells for 30 min at 37 °C with anti-sortilin polyclonal antibodies (10 μg/ml), GST-NGFpro (1 μg/ml), or 100 n m BDNF pro-domain (Alomone) diluted in culture medium.

Techniques: Derivative Assay, SPR Assay, Binding Assay

Journal: The Journal of Biological Chemistry

Article Title: Identification of a Linear Epitope in Sortilin That Partakes in Pro-neurotrophin Binding *

doi: 10.1074/jbc.M109.062364

Figure Lengend Snippet: Mutation of the linear binding site specifically impairs binding of both the NGF and the BDNF pro-domains. SPR analysis showing reduced binding of equal amounts (analyte concentration: 200 n m ) of the soluble extracellular domains of sortilin-4A compared

Article Snippet: Internalization of bound ligands was studied by the incubation of cells for 30 min at 37 °C with anti-sortilin polyclonal antibodies (10 μg/ml), GST-NGFpro (1 μg/ml), or 100 n m BDNF pro-domain (Alomone) diluted in culture medium.

Techniques: Mutagenesis, Binding Assay, SPR Assay, Concentration Assay